細菌轉化 的英文怎麼說

中文拼音 [xìjūnzhuǎnhuà]
細菌轉化 英文
bacterial conversion
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 轉 : 轉構詞成分。
  • 細菌 : germ; bacterium （pl. bacteria）; fungus （pl. fungi）
  • 轉化 : 1. （轉變） change; transform 2. [化學] inversion; conversion
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. Construction and expression of yeast engineering yaccine : s14 / gnsag " as transformed to yeast host straln x33 by means of electroporation after ppiczaa s, . aresag " as l ined by saci enzyme. the single fungus, as choose and dibble inocu1ating in we and am plate, the positive fungus was gro ' ing in rm but not in w, and was 6 inoculated in ypd which included zeocine 500ug / ml and 1000ug / ml. 5 transformers were ampl if ied by pcr, three is same with positive control

    選取單個菌落分別點種到刪平板和md平板，找出在回d上生長正常， w上生長緩慢或不生長的菌落，即陽性菌落，再以陽性菌落分別塗布zeocine含量500ug加， 1000ug砌l的ypd平板，以高濃度的抗生素篩選高拷貝的酵母工程菌，在含500ug ml高濃度抗生素平板上獲得了15個轉化子，取其中5個進行pcr擴增，有3個擴增產物與陽性對照相同，說明此酵母細胞中已含有s hbsag融合片段，其中之一命名為p
  3. Sulfid also can be regarded as a marker of the action of sulfur bacteria. 8 ) based on research results, author postulated that early generation of hydrocarbons is closely related to the action of sulfur bacteria. many kind of algae such as dinoflagellates, diatom, prynesiophytes etc have rich biological lipids which has lower polymerization

    6 、從未熟一低熟源巖生烴組分及其演化、可溶有機質轉化生烴等方面，探討了未熟一低熟油的形成機制，提出本區未熟一低熟油氣的形成是低活化能的富氫腐泥組分受到硫細菌早期低溫降解作用的結果。
  4. A new transgenic system of gerbera mediated by agrobacterium tumefaciens

    小細胞團為受體的根癌農桿菌介導的非洲菊基因轉化體系
  5. Washed air purifier working principle : siphon and using centrifugal principle will be mixed in water pure plant essential oils inhaled through its siphon principle the motor base coaxial centrifugal turbines in the bottom of straw through exchanges cover a very high - speed rotary motor, reuse centrifugal principle, will be mixed in water pure plant essential oil spray bottle in the form within a water film bile, the dust in the air and inhaled bacteria in water purification at the same time after the indoor air insufflation, quickly and efficiently by removing indoor toxin biological, dust, cigarette smoke, the smell, virus

    水洗空氣清新機工作原理：是利用虹吸以及離心原理；將混合於水的純植物精油通過虹吸原理吸入其電機底座的同軸離心渦輪下部的吸管中，通過交流罩極電機高速旋轉，再利用離心原理，將混合於水的純植物精油噴在瓶膽內形成一層水膜，將空氣中的灰塵以及細菌吸入水中，同時將經過凈化的空氣吹入室內，快速有效地去除室內的有毒素生物、灰塵、煙味、臭味、病毒等。
  6. Sub - - clone of s, . / hbsag fusion gene : pbuescripts, . / hbsag and ppiczaa were digested separately by xhoi and xbai enzyme, and were linked under t4 dna ligase, ppiczaa s, / hbsag was constructed and transformed to e. coli

    Hbsag質粒與ppiczaa載體分別經xhol和xbaln切，再在t4dna連接酶作用下進行連接，獲得工程菌表達型ppiczaas ； hbsag質粒，轉化大腸桿菌t0p10細胞，經xhol和xbal與sacll和xbal酶切電泳，證實s ； 。
  7. The recombinant plasmid puge dna and transfer vector pfastbacl dna were treated again in the same enzyme, were linked by means of t4 dna ligase and transformed into e. coli jm109 permissive cells, yielding recombinant transfer vector plasmid pfastbac - ge dna and were transformed into dhlobac containing vector bacmid

    將重組質粒pugedna與轉移載體pfastbacldna用bamhi和ecori雙酶切處理， t _ 4dna連接酶連接，用連接產物轉化大腸桿菌jm109感受態細胞，得到重組轉移載體質粒pfastbac - gedna 。
  8. Inhibitors of cyp51, such as azalanstat ( rs - 2i607 ) and rs - 2i745, could inhibit the synthesis of ff - mas to decrease the accumulation of ff - mas. inhibitor of a14 - reductase, such as ay9944 - a - 7, could inhibit the metabolism of ff - mas to increase the accumulation of ff - mas ; and some other reagents, such as nystatin, could combine with the downstream intermediate in the cholesterol biosynthesis pathway to accumulate mas. in this study, we investigated the role of mas by using these reagents to change the level of endogenous ff - mas

    抑制cyp51酶的抑制劑，如azalanstat （ rs - 21607 ）和rs - 21745等，均能抑制mas的合成，降低mas的積累；而抑制14 -還原酶的抑制劑，如ay9944 - a - 7等，能抑制ff - mas向t - mas的轉化而造成ff - mas的積累；還有一些物質，如制黴菌素，能阻止mas向下游的代謝而造成其積累，本文主要通過應用這些物質降低、或增加組織細胞中內源性mas的積累，來研究mas的作用。
  9. In the stomach, this acid functions to kill bacteria in foods, to soften foods and to convert the inactive enzyme pepsinate into its active from pepsin, to begin the digestion of protein

    在胃裡，這種酸的作用是殺司食品的細菌，使食物軟化，把鈍化的胃蛋白酶原轉化為有活性的能消化蛋白質的胃蛋白酶。
  10. Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115. mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting

    本研究將含有n末端信號肽和c末端毒性區缺失的pap基因的表達載體ppic9k - p用sali酶切線性化后，通過電擊轉化整合p . pastorisgs115菌株細胞中。
  11. The recombinant expression plasmids prt - p450nor and pet - p450nor were constructed by inserting p450nor gene into the bamh i / hind iii site of the prokaryotic expression vector prset and pet28, then they were transformed into e. coli bl21

    本研究將已克隆的真菌細胞色素p450nor基因插入原核表達質粒載體prset和pet28的bamhi / hind位點，成功構建重組表達質粒prt - p450nor和pet - p450nor ，並轉化到e . colibl21 。
  12. Liquid and pore detailed to absorb function through human mouth, change human difference in temperature and body supersession chen, etc. function transform, promote beneficial bacterium activate and get rid of body the dying and positive germ

    經人體口液及毛孔細吸收功能，並受人體溫差變化及身陳代謝等功能轉化，促進有益菌活化並排除體內死化及陽性病菌。
  13. After the protein was electrophoresised and purified, the protein activity was detected by elisa, the protein activity of vp1 is higher than vp0 vp3. at last, the activity of vp1 made in our lab was detected with the agentia made in our lab

    將陽性重組子轉化到大腸桿菌er2566細菌內，用ipig進行誘導表達蛋白，蛋白經電泳、純化，然後用elisa方法檢測蛋白活性， vp1蛋白活性相對高於vp0 、 vp3 。
  14. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。
  15. Producers are those organisms that can build up foods from inorganic mateials, i. e. green plants, algae, and photosynthetic and chemosynthetic bacteia

    生產者是指那些把無機物轉變成有機物的各種生物，包括綠色植物、藻類、光能以及化能合成細菌。
  16. Gfral was high expressed in e. coli after iptg induction. by ni2 + chelation affinity chromatography, the purified recombinant gfral protein were obtained. based on the evolutionary trace method, multiple sequence alignment and dendrogram construction were then carried out by the clustalx program and constructed a phylogenetic tree from a multiple sequence alignment for a protein family

    2 . gdnf的表達及其對pc12基因工程細胞的影響用本教研室已構建好的表達質粒pet一gdnf轉化大腸桿菌bl21 （ de3 ） ，經lmmipto誘導gdnf表達，並在niz氣nta柱上進行純化，稀釋復性后，純度達90 %以上。
  17. The expression vector pse380 - / iy / was constructed and transformed into e. coli dh5a, expressing hyl gene by adding iptg into the broth. the expression of hyl gene showed a 120kda protein band on sds - page gel and was found to have capability to degrade ha molecules derived from a microorganism dissolved in 0. 1 m acetate buffer solution ( ph4. 0 )

    經轉化大腸桿菌dh5a和iptg誘導表達後用sds - page電泳分析，獲得一條約120kda的表達條帶； iptg誘導表達后提取原生質膜測定透明質酸分解酶活力，表明該hyl片段的產物能夠在體外分解細菌來源的ha 。採用兩種策略滅活hyl基因。
  18. But polyadenylation in bacteria needs no specific consensus sequence or there is no such sequence signals found. the sites of polyadenylation of bacterial mrna are diverse, including the 3 ' ends of primary transcripts, the sites of endonucleolytic processing in the 3 ' untranslatd and intercistronic regions, and sites within the coding regions of mrna degradation products

    細菌mrna多聚腺苷酸化的位點多種多樣，包括初級轉錄產物的3 』末端， 3 』端非翻譯區和順反子間區的內切酶加工位點及mrna降解產物的編碼區內，其腺苷酸化相對無特異性、無選擇性。
  19. The results showed that quantity of bacterium and four bacterial physiology groups was positively correlated with quality of illumination ; their quantity showed a reduced tendency with the reducing of the illumination condition, but quantity of fungi was negatively correlated, it was increased gradually with the reducing of the illumination condition ; rhizosphere soil of kentucky bluegrass turned into fungi type from bacterium type ; the rhizosphere effect of various bacterial physiological group of kentucky bluegrass is obvious under different quality of illumination

    結果表明，草地早熟禾根際細菌及四類細菌生理群數量與光照條件呈正相關，隨著光照條件的減弱，其數量呈降低趨勢；根際放線菌數量隨光照的減弱呈先下降後上升的趨勢；而真菌數量與光照條件呈負相關，隨著光照條件的減弱，根際真菌的數量逐漸增加；草地早熟禾根際土壤由「細菌型」向「真菌型」轉化；不同光照條件下，根際各微生物類群都表現出明顯的根際效應。
  20. This paper discusses how the silicate bacteria affect potassium releasing from minerals, especially the function mechanism during the interaction between bacterial and minerals ; the paper emphasize the problem such as the utilization of silicate bacteria to release significant amounts of potassium from soil minerals in the karst area, and at the same time the utilization of the silicate bacteria in the agriculture of karst area is discussed

    主要探討矽酸鹽細菌的解鉀作用，以及使難溶性礦物態鉀轉化為速效性鉀的作用機理；同時在研究矽酸鹽細菌解鉀作用機理問題的基礎上，重點探討了喀斯特環境中利用矽酸鹽細菌活化土壤中的礦物鉀元素的問題，特別是矽酸鹽細菌在喀斯特環境中農業上的利用。
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