終止密碼突變 的英文怎麼說
中文拼音 [zhōngzhǐmìmǎtūbiàn]
終止密碼突變
英文
termination codon mutation- 終 : Ⅰ名詞1 (最後; 末了) end; ending; finish 2 (指人死) death; end 3 (姓氏) a surname Ⅱ形容詞(...
- 止 : Ⅰ動詞1. (停止; 攔阻) stop; cut out 2. (截止) close; end Ⅱ副詞(僅; 只) only; just Ⅲ名詞(姓氏) a surname
- 密 : Ⅰ名詞1 (秘密) secret 2 [紡織] (密度) density 3 (姓氏) a surname Ⅱ形容詞1 (距離近; 空隙小)...
- 碼 : Ⅰ名詞(表示數目的符號或用具) a sign or object indicating number; code Ⅱ量詞1 (指一件事或一類的...
- 突 : Ⅰ動詞1 (猛沖) dash forward; shoot out 2 (高於周圍) protrude; bulgeⅡ副詞(突然) abruptly; sud...
- 終止 : 1 (結束) stop; end; suspend 2 (停止) termination; annulment; abrogation 3 [音樂] cadence; 終...
- 突變 : 1 (突然急劇的變化) sudden change; change suddenly; transilience; accident; saltation; revulsion...
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Pcr method was used to identify candidate ms 188. sequence analysis indicated that a point mutation occurred in the second exon of the atmyb103 gene in male sterile mutant with caa ( gln ) replaced by a stop codon taa
利用pcr的方法從突變體中擴增atmyb103基因並進行序列分析,結果表明突變體中atmyb103基因第二個外顯子上發生了點突變,由原來編碼谷氨酰胺的caa密碼子突變為終止密碼子taa 。Analysis of the sequence variation of cytochrome b gene indicated that there is no evidence of insertions or deletions, i. e., they are all of identical length of 1143 bp in all the sequences of cytochrome b gene. further, the sequences can be fully translated into amino acid using chicken mitochondrial codon without nonsense mutations or intervening stop codons. the 1143 bp cytochrome b alignment contained 416 variable sites, of which 306 were parsimony informative sites with the strongest variable in third codon positions and less variable in first and second codon positions
細胞色素b基因序列變異分析表明: 1 )雁形目鳥類細胞色素b基因全序列長度一致,無插入和缺失:對照雞線粒體密碼子系統全序列能全部翻譯成氨基酸序列,無無義突變,全序列內部無終止密碼子; 2 )序列比對后1143加,含416個核著酸變異位點, 306個簡約信息位點,其中處於密碼子第三位的變異最大,第一位和第二位堿基的變異相對較小。Conclusion we have constructed the expression plasmid pbv220 - hpf4 successfully. 3 ' - utr of h pf4 c dna was deleted and tag was mutated to taataa by pcr. after sds - page and densitometric scan analysis, the expression level of r hpf4 is 25 - 30 %
結論本研究運用pcr定點突變技術,完全去除了hpf4cdna基因3 」端utrat富含區:改用大腸桿菌強串聯終止密碼子taaaataa ,成功構建高效表達克隆pbv220 rhpp4 。
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