終止序列 的英文怎麼說

中文拼音 [zhōngzhǐxùliè]
終止序列 英文
terminator sequence
  • 終 : Ⅰ名詞1 （最後; 末了） end; ending; finish 2 （指人死） death; end 3 （姓氏） a surname Ⅱ形容詞（...
  • 止 : Ⅰ動詞1. （停止; 攔阻） stop; cut out 2. （截止） close; end Ⅱ副詞（僅; 只） only; just Ⅲ名詞（姓氏） a surname
  • 列 : Ⅰ動1 （排列） arrange; form a line; line up 2 （安排到某類事物之中） list; enter in a list Ⅱ名詞1...
  • 終止 : 1 （結束） stop; end; suspend 2 （停止） termination; annulment; abrogation 3 [音樂] cadence; 終...
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. The ipcc endorsed 6, 103 allegations, of which almost 39 per cent were classified as withdrawn, not pursuable or curtailment, and some 24 per cent were minor or trivial complaints settled by informal resolution

    在警監會批簽通過的6 ， 103宗指控中，約有39 %列為撤回、無法追查或終止調查，另有約24 %性質輕微的投訴得以循簡易程序解決。
  3. The ipcc endorsed 6 262 allegations, of which almost 44 per cent were classified as " withdrawn ", " not pursuable " or " curtailed ", and some 25 per cent were minor complaints settled by informal resolution

    在警監會批簽通過的6262宗指控中，約44 %個案列為撤回、無法追查或終止調查，另有約25 %屬性質輕微的投訴，已循簡易程序得以解決。
  4. The ipcc endorsed 6, 213 allegations, of which almost 41 per cent were classified as withdrawn, not pursuable or curtailed, and some 27 per cent were minor or trivial complaints settled by informal resolution

    在警監會批簽通過的6 ， 213宗指控中，約41 %被列為撤回、無法追查或終止調查，另約27 %屬性質輕微的投訴，則得以循簡易程序解決。
  5. Pcr method was used to identify candidate ms 188. sequence analysis indicated that a point mutation occurred in the second exon of the atmyb103 gene in male sterile mutant with caa ( gln ) replaced by a stop codon taa

    利用pcr的方法從突變體中擴增atmyb103基因並進行序列分析，結果表明突變體中atmyb103基因第二個外顯子上發生了點突變，由原來編碼谷氨酰胺的caa密碼子突變為終止密碼子taa 。
  6. Analysis of the sequence variation of cytochrome b gene indicated that there is no evidence of insertions or deletions, i. e., they are all of identical length of 1143 bp in all the sequences of cytochrome b gene. further, the sequences can be fully translated into amino acid using chicken mitochondrial codon without nonsense mutations or intervening stop codons. the 1143 bp cytochrome b alignment contained 416 variable sites, of which 306 were parsimony informative sites with the strongest variable in third codon positions and less variable in first and second codon positions

    細胞色素b基因序列變異分析表明： 1 ）雁形目鳥類細胞色素b基因全序列長度一致，無插入和缺失：對照雞線粒體密碼子系統全序列能全部翻譯成氨基酸序列，無無義突變，全序列內部無終止密碼子； 2 ）序列比對后1143加，含416個核著酸變異位點， 306個簡約信息位點，其中處於密碼子第三位的變異最大，第一位和第二位堿基的變異相對較小。
  7. To inform the exception - handler object that a message is about to be placed on the final resting or dead letter queue

    的方法來通知異常處理程序對象：一條消息被放至最終靜止隊列或死信隊列。
  8. Compared hasnpv helicase with the helicases of autographa colifornica mnpv ( acmnpv ), ( bombyx bori npv ( bmnpv ), lymantria dispar mnpv ( ldmnpv ) spodoptera exigue mnpv ( semnpv ), orgyia pseudotsugata mnpv ( opmnpv ), and xestia c - nigrum granuloviurs ( xcgv ), only 5 motifs ( i, la, ii, iii, iv ) were found conserved in baculovirus

    其在終止密碼子下游第12位有? polya終止信號aataaa 。 hasnpv解螺旋酶與其它5種桿狀病毒解螺旋酶相比，有5個基元序列（ 、 a 、 、 、 ）保守，另外兩個（和）完全不保守。
  9. The amplification system was optimized so that the pcr with different primers can be carried out under the same condition. the pcr products were sequenced using sanger ' s terminator and fluorescent label techniques. sequences were analyzed and compared base on sequencing analysis3. 4 and seq / ede software

    方法根據mtdna控制區及其周圍區域的序列，設計多對引物，探索優化擴增體系，使擴增條件能夠同時滿足多對引物的需要，用sanger末端終止法及熒光標記技術對樣本進行dna測序， sequencinganalysis3 . 4和seq ede軟體進行序列分析和比對。
  10. Besides, vgb gene expression also increased the chitinase secretion. in order to make the vitreoscilla hemoglobin gene express in bacillus subtilis, an inducible promoter ( levansucrase ) was cloned from b. subtilis wb600 and ligated with a promoter - less vgb gene. the resulted gene is called sacvgb and was demonstrated to express in e, coli by sds - page and carbon monoxide binding assay

    由於vgb基因啟動子不能在枯草桿菌中啟動表達，因此，根據已發表的果聚糖蔗糖酶基因（ sacb ）序列設計引物，從枯草桿菌wb600總dna中擴增出該基因的啟動子片段，然後將其與vgb基因編碼區及終止子序列相連，成功地組建了sacvgb融合基因。
  11. The high - enzyme activity has 2 base changes, resulting in long amino acid sequence with native amylase. this inducing method resolved the problem of non - effective induction as in base analogue induction. and the method we used provide a new measure for this kind of work

    高酶活編碼區位點突變導致c -端序列變化和終止子的后移本誘變方法克服了用堿基類似物在體內誘變由於核酸復制酶等的校正作用而造成誘變無效的難題，為基因的誘變找到了一條新途經。
  12. While executing the program, we memorize all the values of each array variable. the analysis will stop if data dependence is found

    動態測試就是在解釋執行程序的同時，記錄串列程序對數組元素的訪問，如果發現數據相關就終止測試。
  13. The sequence encodes an open reading frame of 518 amino acids. there is a transit peptide of 74 amino acids in the n terminal of the putative amino acids sequence coded by the cdna

    該序列長為1758hp ，起始密碼子位於84 86hp ，終止密碼子為1658 166fop ，開放閱讀框長為1557hp ，編碼58個氨基酸序列，其中n末端含有74個氨基酸的轉運肽。
  14. In this research two full - length cdnas have been cloned by a combination of rt - pcr and 3 " - is1 - race with synthesized degenerate primers from young leaves of vicia faba l., pichia methanolica high - level expression systems of the genes have been constructed, and the milligram expressed protein was purified using probond resin purification system, which may result in further identification of the function of the aba binding protein. the full - length cdna of abp370 fragment is 3449 bp long and has an open reading frame of 2304 bp encoding 768 amino acids with 876 bp long 5 ' - utr, 369 bp long 3 ' - utr and poly ( a ) tail. the full - length cdna of abp640 fragment is 1012 bp long and has an open reading frame of 780 bp encoding 260 amino acids with 88 bp long 5 ' - utr, 144 bp long 3 " - utr and poly ( a ) tail

    3 - 5 - race擴增片段序列分析結果表明， abp370擴增片段的全長cdna為3449核苷酸，其中5非翻譯區為876個核苷酸， 3非翻譯區為369個核苷酸並末端帶poly （ a ）尾巴，從起始密碼子atg至終止密碼子tga ，含有一個編碼768個氨基酸殘基的開放閱讀框架（ 2304bp ） ； abp640擴增片段的全長cdna為1012核苷酸，其中5非翻譯區為88個核苷酸， 3非翻譯區為144個核苷酸並末端帶poly （ a ）尾巴，從起始密碼子atg至終止密碼子taa ，含有一個編碼260個氨基酸殘基的開放閱讀框架（ 780bp ） 。
  15. In these composites, the coupling termination content of pmma decreases while disproportionation content increases. the macromolecular ranging regularity of pmma also increases

    上述復合材料中， pmma大分子的終止方式發生偶合終止減少、歧化終止增加的變化，同時pmma整體排列有序度增加。
  16. 3. arabidopsis plants were transformed with the constructed plant binary expression vector. eighteen independent antibiotic - resistant arabidopsis transformants for dreb1c were obtained by using a vacuum infiltration method

    載體全長為13kb ，有一個煙草花葉病毒35s強啟動子和nos終止序列，利用bamhi和sacl做為酶切位點，構建載體。
  17. Then, 5. 5kb thrombiotin gene was amplified with the same technique from the genome of a baby ' s blood, which included the begining part of intronl to the teminator. in addition, 6. 0kb and 1. 8kb homlogous arms were also amplified from a cow with high yield. the 6. 0kb homologous arm contains the promotor, extron 1, extron2, extron3 and intron 1, intron2 and part of the intron3 fragment, while the 1. 8kb homologous right arms contains exon13, exon14 and part of intron 13, the whole intron14 and part intron 14 of asl - casein gene of bovine

    通過長片段pcr從高產奶牛的基因組中獲得了打靶所需的長、短同源臂序列，長度分別為6 . 0kb和1 . 8kb ，位於s1 -酪蛋白基因的5上游區到第三內含子和十二到十四內含子；從綿羊全血基因組克隆得到了綿羊的-酪蛋白基因啟動子區到第二內含子區4 . 1kb的5調控序列；利用同對引物克隆得到了水牛的同基因序列；從廣西當地一嬰兒臍血基因組中通過獲得了人血小板生成素基因，位於第1內含子到終止子後部分的序列，長達5 . 5kb 。
  18. Ln our experiment, a lot of regulator sequences inc1uding the 35s promoter with double enhancer e1ements and the terminator nos, the fl sequence and kozak sequence for improving the genetic statement in the level of translation, were appended on the two s ides of the artifically pseudopleuronectes americanus antifreeze protei n gene by a series of process, and the products were linked into pbil21

    本實驗中人工合成了美洲擬鰈抗凍蛋白基因，並在美洲擬鰈抗凍蛋白基因兩側添加了許多調控序列，如具有加倍增強子的35s啟動子和nos終止子，它們可以促進抗凍蛋白基因的轉錄，及可在翻譯水平上提高基因表達的序列和kozak序列，從而構建了抗凍蛋白基因的高效表達盒。
  19. After sequencing of 84 cdna clones and removing redundant cdnas, we obtained 36 cold - regulated unique cdna clones. 12 cdna clones were expected to be novel genes, because no sequence homology with any known sequences was found in genbank databases

    全長基因ej175共有603bp ，對其可讀閱讀框架進行分析，從57 - 515位核苷酸的一段序列，包含了起始密碼子和終止密碼子，編碼152個氨基酸的多肽。
  20. The low - enzyme activity gene has 2 base changes, resulting in short amino acid sequence with native enzyme

    其中低酶活基因編碼區內一突變位點導致氨基酸序列的提前終止。
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