終止碼 的英文怎麼說

中文拼音 [zhōngzhǐmǎ]
終止碼 英文
stop code
  • 終 : Ⅰ名詞1 （最後; 末了） end; ending; finish 2 （指人死） death; end 3 （姓氏） a surname Ⅱ形容詞（...
  • 止 : Ⅰ動詞1. （停止; 攔阻） stop; cut out 2. （截止） close; end Ⅱ副詞（僅; 只） only; just Ⅲ名詞（姓氏） a surname
  • 碼 : Ⅰ名詞（表示數目的符號或用具） a sign or object indicating number; code Ⅱ量詞1 （指一件事或一類的...
  • 終止 : 1 （結束） stop; end; suspend 2 （停止） termination; annulment; abrogation 3 [音樂] cadence; 終...
  1. Methods and applications of inserting a stop codon into a cloned gene with pcr

    在已克隆基因末端添加終止密碼的方法與應用
  2. Chain termination codon

    鏈終止密碼子
  3. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  4. The start reading framae and stop codons, base composition in protein - coding genes and the codon usage of amino acids in scolopendra multilane were compared with the three other myriapods

    本研究在蛋白質編碼基因起始閱讀框和終止密碼子、蛋白質編碼區的堿基組中文摘要成、氨基酸及密碼子的利用等方面把少棘蜈蚣與另三種多足類進行了比較。
  5. J mol biol., 1992, 224 : 53 - 63. 26 abe h, aiba h. differential contributions of two elements of rho - independent terminator to transcription termination and mrna stabilization. biochimie, 1996, 78 : 1035 - 1042

    通過計算，我們預測到266個不依賴終止子，其中包括232個蛋白編碼基因， 12個trna基因和3個rrna基因，約17 %不依賴終止子位於操縱子的末端。
  6. Sv40 polya signal and two loxp sites was added consecutively after the termination codon taa, and then a gfp gene was inserted between the two loxp sites

    在三個終止密碼子taa之後，連上sv40polya 。 sv40polya之後，又連上兩個loxp位點。在兩個loxp位點，插入標記基因gfp 。
  7. A late baculoviral transcription initiation motif ataag was found 65 nt upstream of the putative translational start site and a polyadenylation signal aataaa was identified 14 nt downstream of the taa stop codon

    在其起始密碼子上游- 65nt發現桿狀病毒晚期啟動子轉錄起始信號ataag ，在其終止密碼子下游- 14nt發現polya加尾信號aataaa 。
  8. Pcr method was used to identify candidate ms 188. sequence analysis indicated that a point mutation occurred in the second exon of the atmyb103 gene in male sterile mutant with caa ( gln ) replaced by a stop codon taa

    利用pcr的方法從突變體中擴增atmyb103基因並進行序列分析，結果表明突變體中atmyb103基因第二個外顯子上發生了點突變，由原來編碼谷氨酰胺的caa密碼子突變為終止密碼子taa 。
  9. Analysis of the sequence variation of cytochrome b gene indicated that there is no evidence of insertions or deletions, i. e., they are all of identical length of 1143 bp in all the sequences of cytochrome b gene. further, the sequences can be fully translated into amino acid using chicken mitochondrial codon without nonsense mutations or intervening stop codons. the 1143 bp cytochrome b alignment contained 416 variable sites, of which 306 were parsimony informative sites with the strongest variable in third codon positions and less variable in first and second codon positions

    細胞色素b基因序列變異分析表明： 1 ）雁形目鳥類細胞色素b基因全序列長度一致，無插入和缺失：對照雞線粒體密碼子系統全序列能全部翻譯成氨基酸序列，無無義突變，全序列內部無終止密碼子； 2 ）序列比對后1143加，含416個核著酸變異位點， 306個簡約信息位點，其中處於密碼子第三位的變異最大，第一位和第二位堿基的變異相對較小。
  10. Compared hasnpv helicase with the helicases of autographa colifornica mnpv ( acmnpv ), ( bombyx bori npv ( bmnpv ), lymantria dispar mnpv ( ldmnpv ) spodoptera exigue mnpv ( semnpv ), orgyia pseudotsugata mnpv ( opmnpv ), and xestia c - nigrum granuloviurs ( xcgv ), only 5 motifs ( i, la, ii, iii, iv ) were found conserved in baculovirus

    其在終止密碼子下游第12位有? polya終止信號aataaa 。 hasnpv解螺旋酶與其它5種桿狀病毒解螺旋酶相比，有5個基元序列（ 、 a 、 、 、 ）保守，另外兩個（和）完全不保守。
  11. To prevent the finalization code from executing a second time

    以防止終止代碼執行第二次。
  12. Besides, vgb gene expression also increased the chitinase secretion. in order to make the vitreoscilla hemoglobin gene express in bacillus subtilis, an inducible promoter ( levansucrase ) was cloned from b. subtilis wb600 and ligated with a promoter - less vgb gene. the resulted gene is called sacvgb and was demonstrated to express in e, coli by sds - page and carbon monoxide binding assay

    由於vgb基因啟動子不能在枯草桿菌中啟動表達，因此，根據已發表的果聚糖蔗糖酶基因（ sacb ）序列設計引物，從枯草桿菌wb600總dna中擴增出該基因的啟動子片段，然後將其與vgb基因編碼區及終止子序列相連，成功地組建了sacvgb融合基因。
  13. If a reconnection request is not made before date of termination, we shall have the right to dispose of your e - mail address ( es ), user id ( s ) andor password ( s ) at our sole discretion without notice and without liability to you

    倘閣下並無于終止日期前要求重新連接，吾等有權處理閣下之電郵地址、用戶編號及或密碼，而毋須通知閣下或向閣下負責。
  14. The high - enzyme activity has 2 base changes, resulting in long amino acid sequence with native amylase. this inducing method resolved the problem of non - effective induction as in base analogue induction. and the method we used provide a new measure for this kind of work

    高酶活編碼區位點突變導致c -端序列變化和終止子的后移本誘變方法克服了用堿基類似物在體內誘變由於核酸復制酶等的校正作用而造成誘變無效的難題，為基因的誘變找到了一條新途經。
  15. " we ( atv and tvb ) believe that it would be better to have a cautious start than rushing into a decision that we might regret later. as the saying goes, haste makes wastes, " said mr. kwong. " what would most benefit the consumers, competition, investors and hong kong s reputation would not be who started dtt first, but who has the best possible plan to start with, and could maximise the potentials of digital tv in the end

    我們相信，謹慎的開始較匆忙決定而引起將來後悔為佳，所謂欲速不達。況且達者為先，對于消費者、投資者及香港的聲譽而言，最有利的不是誰先開始，而是誰有較佳的計劃開始數碼地面電視，最終能充份發揮數碼電視之潛在力。簡而言之，最為重要的是誰能率先終止模擬制式廣播。
  16. In the decoder, an end - of - block ( eob ) position of an incoming block received by the decoder is determined and a discrete cosine transform ( dct ) block type is determined based on the determined eob position

    在該解碼器部分，將收到的輸入區塊中決定一區塊終止的位置，並根據此區塊終止位置決定一種離散餘弦轉換區塊形式。
  17. Using 3 ' race the transcription stop site mapped 27nt downstream of the putative translation stop codon

    3 』 race分析結果表明轉錄的終止位點在翻譯終止密碼子下游的27個堿基處。
  18. Using an 8 - depth async fifo solves the synchronization and exchange of data be - tween different clock domains. the data transaction protocol comes from the most basic work way of uart. when the master clock is 16. 7mhz, the pcm side and adpcm side clocks both are 2. 38mhz, the results of simulation show that the latency from the start - bit of pcm data inputting uart receiver to the stop - bit of adpcm data outputted uart transmitter is 14. 3 us and the latency from the start - bit of adpcm data inputting uart receiver to the stop - bit of pcm data outputted uart transmitter is 14. 7 us

    在主時鐘為16 . 7mhz ， pcm數據端與adpcm數據端時鐘均為2 . 38mhz時，模擬結果表明從pcm的起始位輸入uart接收器到adpcm終止位輸出uart發送器的最大延遲為14 . 3 s ，從adpcm的起始位輸入uart的接收器到pcm終止位輸出uart發送器的最大延遲為14 . 7 s ，設計時盡可能的使編碼與解碼的時間相差不多，從結果看出基本達到這個要求。
  19. Conclusion we have constructed the expression plasmid pbv220 - hpf4 successfully. 3 ' - utr of h pf4 c dna was deleted and tag was mutated to taataa by pcr. after sds - page and densitometric scan analysis, the expression level of r hpf4 is 25 - 30 %

    結論本研究運用pcr定點突變技術，完全去除了hpf4cdna基因3 」端utrat富含區：改用大腸桿菌強串聯終止密碼子taaaataa ，成功構建高效表達克隆pbv220 rhpp4 。
  20. Code, end - of - loop

    環終止碼
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