組分濃度梯度 的英文怎麼說

中文拼音 [zǔfēnnóngdùtīdù]
組分濃度梯度 英文
compositional gradient
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  • 濃 : 形容詞1. （液體或氣體中所含的某種成分多; 稠密） dense; thick; concentrated 2. （程度深） （of degree or extent） great; strong
  • 度 : 度動詞[書面語] （推測; 估計） surmise; estimate
  • 梯 : Ⅰ名詞1 （梯子; 樓梯） ladder; stairs; steps; staircase 2 （姓氏） a surname Ⅱ形容詞（形狀像樓梯的...
  • 濃度 : potency; thickness; concentration; consistence; strength; consistency; density
  1. In this paper, kandelia candel ( l. ) druce hypocotyls were cultivated in sand and treated with 15 % seawater for 60 days under laboratory conditions. the influence of increasing concentrations of napthalene and pyrene ( 0, 0. 1, 1 and 10mg / l ) on hypocotyl germination and growth, photosynthesis metabolism, water metabolism and membrane protection system were observed to inquire into the ecophysiological responses of mangrove k. candel to pahs phytotoxicity. moreover, the concentration and distribution of parent polycyclic aromatic hydrocarbons ( pah ' s ) in surface sediment, underground root and leaf residue of mangroves in jiulong river estuary ( fugong, north and south shores of haimen island, baijiao ), neighbouring xiamen western harbour ( dong islet ) and dongzui harbour ( fenglin ) were examined

    在實驗室條件下，分別以0 . 1 、 1和10mg l的萘（ nap ）和芘（ pyr ） 3個梯度濃度組級砂基培養秋茄（ kandeliacandel （ l . ） druce ）幼苗，培養基鹽度15 ，培養期60d ，以不加pahs為對照，分析了nap和pyr對紅樹植物秋茄幼苗的生長、光合代謝、水分代謝以及膜保護系統的影響，探討pahs對紅樹植物秋茄的的生理生態效應及植物性毒害（ phytotoxicity ）的機理。
  2. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  3. This study we acquired the coding region of hcv ns5b gene by pcr of hcv full length genome and construct the recombinant plasmid pegep n3 - ns5b ; with the different concentration of g418 in the culture medium, we think the selection concentration of g418 for hepg2 cell is 800 g / ml ; the recombinant plasmid was transfected into hepg2 cell by lipofectamine2000 cells containing stable transformants were selected by the ability of resistance to g418 and isolated with the limited dilution. the stably transfected cell line expressing ns5b - egfp fusion protein was obtained by the detection under fluorescence microscope and rt - pcr

    本研究首先從hcv基因組中擴增出nssb基因，構建了nssb基因與報告分子egfp （增強型綠色熒光蛋白）基因的融合基因真核表達質粒pegfpn30 ；通過含有不同g418濃度梯度的培養液培養hepgz細胞，確定了篩選用g4181作濃度為800pg ml ；利用脂質體法將該重組質粒轉染hepgz細胞，經過有限稀釋法和g4壓力選擇，應用熒光顯微鏡和rtpcr檢測，獲得可穩定表達nssbegfp融合蛋白的hepgz細胞克隆。
  4. Because drug molecules are rapidly removed by the systemic circulation and distributed into a large volume of body fluids and tissues, drug concentration in blood is initially low compared with that at the administration site, producing a large gradient

    由於藥物分子是經體循環快速轉運並分佈到大容積體液和組織中去的，所以開始時，血液中的藥物濃度低於給藥部位的藥物濃度，形成大的濃度梯度。
  5. One group ( the soft water group ) of fish were exposed to the cd2 + concentration of 0. 00 mg / l 0. 06 mg / l 0. 30 mg / l1. 50 mg / l, the other group ( the hard water group ) were exposed to the same cd2 + concentration but increase the hardness of water to 16 degree ( germany degree ) by add calcium chloride into the solution

    實驗中將草魚置於鎘濃度分別為0 . 06mg l 、 0 . 3mg l 、 1 . 5mg l的水溶液中，每個梯度又分成軟水組和硬水組，同時設軟水對照和硬水對照，共8個組。軟水組為自來水，硬水組為通過向水中加入cacl _ 2 ，使其硬度為16度（德國度） 。
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