組織化蛋白質 的英文怎麼說
中文拼音 [zǔzhīhuàdànbáizhí]
組織化蛋白質
英文
texture protein- 組 : Ⅰ名詞1 (由不多的人員組成的單位) group 2 (姓氏) a surname Ⅱ動詞(組織) organize; form Ⅲ量詞(...
- 織 : 動詞(編織) knit; weave
- 蛋 : 名詞1. (鳥類或龜、蛇類所產的卵) egg 2. (像蛋形的東西) an egg-shaped thing 3. (辱罵之詞)
- 白 : Ⅰ形容詞1 (似雪的顏色) white 2 (清楚; 明白; 弄明白) clear 3 (空的; 沒加他物的) pure; clear; ...
- 質 : Ⅰ名詞1 (性質; 本質) nature; character; essence 2 (質量) quality 3 (物質) matter; substance;...
- 組織化 : organization
- 組織 : 1 (組織系統) organization; organized system 2 (組成) organize; form 3 [紡織] weave 4 [醫學] [...
- 蛋白質 : [生物化學] protein; proteide (舊稱「朊」, 由多種氨基酸結合而成的高分子化合物)
- 蛋白 : 1. (卵中透明的膠狀物質) egg white; albumen; gary2. [生物化學] (蛋白質) protein
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Female mice serum and vaginal secretion antibodies, their fertility and the pathological changes were determined. on the other hand, the effects of the anti - serum against synthetic peptide on the sperm - egg interaction during ivf and the location of p3 peptide in sperm were watched. the main results and conclusions were as follows : 1 ) the new antigen p3 peptide which harvested from the 430a peptide synthesizer were verified by the mass spectrograph and hplc, that the ratio of mass / charge of the synthetic peptide was equal to the molecular weight in theory and its purity was above 95 %
本研究的主要結果和結論如下:一、經抗原分子設計,在430a自動肽合成儀上合成的新抗原p3 ,經質譜儀分析證明其荷質比與理論一致;純化后,其純度大於95 ;將新抗原與klh偶連后免疫小鼠,經westernblot證實其誘導的特異性抗體可識別小鼠、大鼠、人睪丸組織中分子量約為22kd和55kd左右的蛋白質。Conclusion : phosphoprotein enrichment technique combined with two - dimensional gel electrophoresis is an effective approach to study phosphoproteome, laying a foundation for further investigation of human kidney phosphoproteins
結論:磷酸化蛋白富集技術與二維凝膠電泳技術的結合是研究磷酸化蛋白質組的有效方法,為進一步研究人腎組織磷酸化蛋白奠定基礎。O - quinones then produce the well documented brown color by reacting to form compounds with amino acids or proteins, or they self - assemble to make polymers
然後,這些無色化合物就會自我組合為聚合物,或與氨基酸或蛋白質發生反應,在蘋果組織內生成一些深色的次級產物。This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected
本研究以蛋白質分子設計的理論和方法研究避孕疫苗,將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合,獲得新抗原- 35肽序列;並在合成、純化後分別與弗氏佐劑、免疫刺激復合物( iscoms )混合后免疫不同遺傳背景的雌性小鼠,觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變:以及在ivf下,新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。Ceviar living cells, which undergo devision 200 times faster than normal cells, contain a great variety of activated factors that stimulate fission of both organism and cells, and regeneration of cells, improving micro circulation and supply of nutrients, reducing melanin, and thus growing vitality and skin springiness, resulting from less wrinkles and shrinking pores
魚子活細胞有旺盛的生命力,它的細胞分裂頻率比成熟細胞強200倍,內含多種活性因子,可啟動人體組織細胞的分裂和活化,刺激老化細胞更新再生,改善微循環,淡化黑色素,其高分子蛋白質有效的補充人體細胞和皮膚細胞的養份,增強機體活力和皮膚的彈性,修護鞏固基底層細胞組織達到去除皺紋、收緊毛孔、提升皮膚的顯著美容效果。This thesis is consists of three parts : 1. the purification of ucp - l ( uncoupling protein - 1 ) from bat ( brown adipose tissue ) of the tree shrew. 2
本論文主要內容有三個部分: 1 、中緬樹?褐色脂肪組織解偶聯蛋白質- 1的純化。Methods : hyperosmotic pressure animal model was established by administering 3 % sodium chloride as drinking water to rats or increasing osmotic pressure of the culture medium. osmoregulation positions in the brain, reciprocal projection pathways between the medullary visceral zone ( mvz ) and supraoptic nucleus ( son ) or hypothalamic paraventricular nucleus ( pvn ), oscillation of intracellular calcium in cultured neurons and astrocytes were studied by means of anti - fos, glial fibrillary acidic protein ( gfap ), tyrosine hydroxylase ( th ) or vasopressin ( vp ) multiple imrnunohistochemical staining, immuno - electronic microscope, wga - hrp retrogradely tracing and cell culture methods. results : ( 1 ) fos positive neurons within the mvz, parabrachial nuclei, locus ceruleus, pvn, son, subfomical organ increased markedly
方法:通過給予大鼠飲用3氯化鈉或提高培養基滲透壓濃度的方法復制高滲刺激模型,主要採用抗fos 、膠質原纖維酸性蛋白( gfap )和酪氨酸羥化酶( th ) (或加壓素? vp )免疫組織化學多重染色、免疫電鏡、 wga - hrp束路追蹤結合免疫組織化學多重染色、細胞培養等實驗方法,系統觀察了中樞參與滲透壓反射的調控部位、下丘腦視上核( son )神經元? ast超微結構的變化、延髓內臟帶( mvz )和son及下丘腦室旁核( pvn )之間往返投射通路和神經元的性質及其與ast的關系、培養神經元和ast內鈣波的變化。Add the bating agent and emulsifier de - fat agent into the same water bath after bating to get rid of the non - structured collagen and non - collagen proteins to enhance the softness of the leather and the elasticity of the surface. it may also increase the deliming effect, improve the degree of cleanness and smoothness of the leather face. the ferment is solved ( soften )
在脫灰后的同水浴中,添加酵解劑和乳化脫脂劑,以除去非組織膠原( nonstructuredcollagen )和非膠原蛋白質( noncollagenproteins ) ,以促進皮質的柔軟度和皮面的彈性,亦可增進脫灰的功效,改進皮面的清潔度與平滑。In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company
實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 ( 1 : 1 )混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑( upofectalnineplusreageni )和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 ( do一7 )蛋白(即用型) ;鼠單克隆抗體p21waf , (即用型) ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰(濃縮型) ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。Under the same condition, the tissue extract of earthworm and the tissue protein extract of earthworm could decompose the pbv220 - r - inf completely but the tissue extract of earthworm without protein could n ' t. 2
雙胸蚓組織粗提取液及雙胸蚓組織的蛋白提取液在37oc保溫1小時可以完全消化pbv220 p一inf質粒而雙胸蚓組織去蛋白提取液對pbv220 y一wf質粒無消化作用。Immunohistochemistry method was used to observe the temporal and spatial expression of nmdar2, signal molecules, skeleton proteins and connexins in son neurons and glias ( astrocytes and microglias ). radioimmunoassay was used to detect vasopressin ( vp ) content in plasma before and after hyperosmotic stimulation. ultrastructure between activated son astrocytes and neurons was observed by double immune - electron - microscopic staining method
應用免疫組織化學方法光鏡下觀察高滲刺激后,大鼠視上核膠質細胞(星形膠質細胞和小膠質細胞)受體( nmdar2 ) 、信號分子、骨架蛋白及縫隙連接蛋白的表達的時空變化;應用放免測定檢測高滲刺激前後血漿中vp含量。Correlation analysis was done using the % vol of matched spots from the two group, which is a useful function offered by the software. consequently, the correlation coefficients of the plc - 1 + / + group were 0. 858, 0. 867, 0. 875, yet the other were 0. 842, 0. 846, 0. 863. we cut out 7 bad - matched spots ( exist in one group and out of the other ) after analysis by auto matching and manual correction, three of which has been analyzed by pmf up to now. of the three analyzed, one has been idendified as vimentin definitely, while the other two can not be identified
經過軟體分析和手工修正,挑取了7個在兩組凝膠中不能匹配的蛋白質斑點(一組存在而另一組消失) ,目前已對其中3個進行了質譜鑒定,其中一個確切鑒定為vimentin ,而另外兩個不能得到鑒定。 vimentin是一種第三類中間纖維,與c - fos 、 c - jun和creb具有較高的同源性,在細胞分裂時常常大量地重新組織,發生顯著的磷酸化。Tissue sections from every animal were double - labeled with the antibodies of map - 2, cox - 2, gdnf, caspase - 3 and either the neuron - specific antibody neuronal nuclear protein ( neun ) or the astroglial - specific marker glial fibrillary acidic protein ( gfap ). we carried out a series of research to explore the effects and mechanism of map - 2, cox - 2, gdnf, caspase - 3 during tbi and trie d to provide some useful theory basis for both the treatment of tbi in the practice and forensic medicine
並通過上述指標分別與神經元特異性標志物神經元核蛋白( neuronalnuclearprotein , neun )和星形膠質細胞特異標志物膠質纖維酸性蛋白( glialfibrillaryacidicprotein , gfap )進行免疫組織化學雙染色,探討腦損傷后神經元及神經膠質細胞反應性變化情況及其分子生物學機制,以期為腦損傷研究提供有益的數據材料,也為以上指標在法醫學實際檢案的應用提供必需理論依據。After stimulation of insulin > epinephrine in 10 ~ 6m to the differentiated 3t3 - l1 cells for 48h, or incubated with 16. 8mm glucose for 48h, the crude membrane proteins were extracted, then investigated the effects of various factors on aqpap protein expression by western blotting. the subjects were divided into three groups according to body mass index : the normal, the overweight, the obese. the expression of adipose aqpap were detected with rt - pcr and western blotting
2 、利用半定量rt - pcr及westernblotting技術,從基因及蛋白質表達水平研究激素(胰島素、腎上腺素、地塞米松、 igf - 1 ) 、營養物質(葡萄糖、甘油) 、調脂藥(非諾貝特、洛伐他汀)對3t3 ? l1脂肪細胞aqpap表達的影響,並觀察不同體重研究對象脂肪組織aqpap表達量的變化。Neurotrophic factors ( ntfs ) are a group of cytokines, which all play important roles in the differentiation, development, growth and regeneration of nerve system
神經營養因子( neurotrophicfactors , ntfs )是一類由神經元、神經支配的靶組織或膠質細胞產生的能促進中樞和外周神經分化、生長和存活的活性蛋白質。The expression patterns of soluble proteins, peroxidase and esterase isozymes were analysed on globular embryogenesis in tissue cultures of alfalfa, it was concluded that from embryogenic callus to globular embryo genesis h peptides emerged and disappeared orderly, the patterns of peroxidase isozyme changed dramatically, esterase isozymes changed slightly while the total enzyme activity was important to keeping the potential of somatic embryogenesis
摘要試驗在苜蓿組織培養中,對球形胚形成過程中特異蛋白質表達的模式、過氧化物酶及酯酶同工酶酶譜變化進行研究,結果表明:苜蓿組織培養中從胚性愈傷組織到球形胚發育的進程中,順序消失和出現了11種中小分子量多肽;過氧化物酶同工酶酶譜發生了顯著的變化;酯酶同工酶酶譜變化不大,但其總活力對于維持體細胞胚胎發生是必須的。Interferon is gens protein, - interferon calls leucocyte interferon again, it is to treat the remedy with chronic second liver, third most effective liver at present, it is reached through restraining virus to duplicate adjust immune function, make serum transaminase returns to normal, improve liver histology to change, prevent the happening of liver cirrhosis and liver cancer, achieve the goal that raises patient life quality and survival rate
干擾素是一族蛋白質, -干擾素又稱白細胞干擾素,是目前治療慢性乙肝、丙肝最為有效的藥物,它通過抑制病毒復制及調節免疫功能,使血清轉氨酶恢復正常,改善肝組織學變,防止肝硬化和肝癌的發生,達到提高病人生活質量和生存率的目的。And after 28 day in the cold acclimation ( 4 ), the content of the ucp - 1 in the mitochondrion, the weight of the bat and the total content of the protein in the mitochondrion began to smooth
冷馴化后,中緬樹? ucp1含量、線粒體總蛋白質含量及褐色脂肪組織重量顯著增加。所以,冷馴化是影響哺乳動物ucp1含量變化的有效因子。Raw materials muscle tissue of amino acids, peptides, dissolved in broth, to increase fresh ; the resilience of the collagen in the connective tissue protein in the long heated complete hydrolysis into soluble gelatin, conducive to digestion ; bone tissue of calcium and vd, the reaction of organic acids, which will help absorb : fat group weaving in and cooking fatty acids can occur in the reaction of the alcohol esters, which will help increase incense
原料肌肉組織中的氨基酸、多肽等溶解于湯汁中,利於增鮮;結締組織中堅韌的膠原蛋白質在長時間加熱后完全水解成可溶的明膠,利於消化;骨骼組織中的鈣質與vd 、有機酸類發生反應,利於吸收:脂肪組織中的脂肪酸則可以與料酒中的乙醇發生反應生成酯類物質,利於增香。Non - model plant proteomics research progress is summarized, it includes non - model plant individual and population proteomics research, tissue and organ proteomics research, subcellular proteomics, plants respond to environment proteomics and biotic environment factor proteomics research, and different perspectives of plant proteomics are also discussed in this paper
概述了非模式植物蛋白質組學的研究進展,主要包括非模式植物個體及群體蛋白質組學,組織和器官蛋白質組學,亞細胞蛋白質組學,響應環境變化的蛋白質組學以及非模式植物生物環境因子的蛋白質組學的研究情況,同時對植物蛋白質組學的發展前景進行了展望。分享友人