線性質粒 的英文怎麼說

中文拼音 [xiànxìngzhílì]
線性質粒 英文
linear plasmid
  • 線 : 名詞1 （用絲、棉、金屬等製成的細長的東西） thread; string; wire 2 [數學] （一個點任意移動所構成的...
  • 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
  • 質 : Ⅰ名詞1 （性質; 本質） nature; character; essence 2 （質量） quality 3 （物質） matter; substance;...
  • 粒 : Ⅰ名 （小圓珠形或小碎塊形物） small particles; grain; granule; pellet Ⅱ量詞（用於粒狀物）
  1. In the end, influence of grain size on the magnetic properties of the samples in the room temperature is studied by the magnetic hysteresis loop

    最後，還研究了幾種納米晶微粒的晶粒線度對其磁學性質的影響。
  2. On the other hand, - 6 fatty acid desaturase injection assay will be performed to check whether a metabolic pathway is established. methords of research three plasmids vector with expression elements are used to establish a eucaryotic expression vector by restriction enzyme cutting and ligation. this vector is used to pronucleus microinjection

    本實驗以pegfp - n1質粒為骨架載體，用酶切連接的方法構建一個順序含有- actin啟動子、 fad2cdna 、 sv40polya加尾信號的真核表達載體，雙切線性化后回收，使用回收的表達載體經原核顯微注射生產轉基因小鼠。
  3. The retention tune of each of the three main peaks of the antibiotic fr - 008 is entirety identical to candicidin, except for one peak ( fr - 008d ), which can also be seen as a very weak signal on the hplc profile of candicidin with the same rt

    因此可以推測，這兩個菌株在表型上的差別有可能與鏈黴菌fr - 008中的線性質粒有直接的關系，但也不能排除染色體基因之間的差異。
  4. The powder was then applied to a silica gel column chromatography charged with 80 % acetone in water and eluted with the same solvent. after concentrating and drying, relatively pure fr - 008 was obtained. hplc assay a comparison between antibiotic fr - 008 and candicidin has been made by studying the hplc separation profiles of antibiotic fr - 008, candicidin and a mixture of both

    對鏈黴菌fr - 008和灰色鏈黴菌imru3570的rflp分析發現，它們的染色體具有很高同源性； pfge的比較研究發現，這兩個菌株的明顯區別是鏈黴菌fr - 008攜帶有兩個線性質粒，而在灰色鏈黴菌imru3570中則沒有這兩個線性質粒。
  5. After linerization, the recombinant plasmid was transformed into pic hia pastoris by electroporation, which then were cultured in md plate free of histidine, from which the positive colones were propagated

    重組質粒線性化后，用電擊法將重組質粒轉化入巴氏畢赤酵母，在缺組氨酸的md板上篩選陽性菌落，然後用不同濃度的g418 ? ypd板篩選多拷貝插入單菌落。
  6. We have summarized a set of theoretical approach to this problem and discussed the instability of the gas cloud and the systems composed of collisionless particles in the background of dark matter. linear perturbation theory is the method to solve this problem, in which, we add the perturbation into the equilibrium state and discussed its evolution

    本文研究了自引力系統的穩定性問題，總結了解決該問題的一套理論方法。並具體研究了存在暗物質背景時氣體雲和無碰撞粒子系統中擾動的演化情況。解決穩定性問題的方法用線性擾動理論，即在平衡態中加入擾動，研究擾動在系統中的演化情況。
  7. The basic principle, main properties, typical parameters, technical characteristics and general situation of klystron are introduced. the electron beam prebunching in the modulated cavity and shift tube of relativistic klystron amplifer ( rka ) is studied analytically, a self - consistent equation of radiation generated by the prebunched electron beam in the radiation cavity is derived using the field method of particle ? wave interaction instead of the electrical circuit method, and in terms of it, the gain in the linear regime calculated, a field analysis method is proposed. the theory analysis shows that the characteristic parameters, such as resonance frequency, real part of gap - impedance, external quality fadtor in all kinds of klystron output circuits including single - beam, multi - beam, single - gap, multi - gap, single - beammulti - gap, multi - beam multi - gap klystron output circuit, can be calculated by the field analysis method

    本文系統的介紹了速調管的工作原理、主要特點、發展概況、主要性能指標和技術特點，解析的研究了電子束在相對論速調管放大器的調制腔和漂移管中的預群聚;用粒子波互作用的場方法導出了在輻射腔中預群聚電子束產生輻射的自洽方程，同時對線性區的增益進行了計算。理論分析表明，場分析法可用於計算單注單間隙、多注多間隙、單注多間隙和多注多間隙速調管輸出迴路的諧振頻率、間隙阻抗實部和外觀品質因數等特性參數。
  8. The ligation products were transfected to jm109 competent cells. the positive clones were confirmed by pcr, then the positive plasmids were minipreped, digested and sequenced

    線性化質粒，分別用竹和sp6rna多聚酶，摻人3 ， s一dutp ，體外轉錄正義和反義探針。
  9. Powders properties of different phases such as the granularity, the shape and the component have been analyzed by the methods of sieving, gravity sedimentation, spectrophotometry, scan electronic microscope ( sem ), transmission electronic microscope ( tem ) and x - ray diffraction ( xrd ). the results show that technic of ball milling and parameters of heat treatments are important influencing factors to properties of cu - zn powders

    採用篩分法、重力沉降法、分光光度法、掃描電鏡法、透射電鏡法和x射線衍射法對不同階段的銅鋅粉末的粒度、形貌、成分等性質進行了分析，結果表明：球磨工藝及熱處理參數對銅鋅粉的性能有重要影響。
  10. The study solves engineering problem as follows : ( 1 ) the systematic study on the historical course of xigeda strata deposit and geologic environment includes strata, lithologic characters and constitution et in the liangshan and panzhihua region. the results show that the xichang - panzhihua express way is controlled by the anninghe fault and the xigeda fault, the earthquake frequently happen in the region of pass, the different sedimentation number is more great, they effect the road building, safety in operation and structure belong road ( 2 ) because in some place the xigeda strata is foundation and roadbed, the study on the basic properties of the xigeda strata include density, moisture content plastic and liquid limit graduation. the results show that the xigeda strata is deposition in lake, the main component is mudstone and sandstone, the fine particle is main, it has some viscosity, the moisture content of mudstone is different the it of sandstone, the other property are likeness. the xigeda strata has no dilatation on total, some claystone have low and medium dilatation ; ( 3 ) the study on the basic properties of xigeda filler include the composition of matter the biggest standard dry density, the optimum moisture content the results show that xigeda filler can use as road material, it is well grade filler and admixture filler on essence, its compaction index should change in different place because the property is controlled by mudstone and sandstone that change is great in different place ; ( 4 ) the study on the xigeda filler craft used as express way roadbed, includes suitable thickness, compaction numbers and methods and equipment choice et

    公路沿線廣泛分佈有昔格達地層，昔格達地層能否用作高速公路路基填料，國內沒有先例，本論文就是結合導師的科研項目，在非典期間，現場長達5個月的工作，完成了從試驗、現場施工工藝到路堤分層沉降監測等工作，是面對生產實際，認識昔格達地層的性質和其作為地基、路基和填料應用中面臨的問題進行的研究。主要的研究內容有：對攀西地區地層巖性、構造等地質環境和昔格達地層的沉積歷史過程作了系統的分析，研究表明西攀高速公路主要受安寧河斷裂和昔格達斷裂影響，通過地區地震活動頻繁，差異沉降較大，對公路建設、安全運行和沿線構築物設計有較大影響；由於昔格達地層在有些路段作為地基、有些作為路基，對其基本性質進行了研究：包括天然密度、含水量、塑液限、顆粒級配等指標的試驗研究。研究成果表明：昔格達地層為湖相沉積，主要為砂巖和泥巖，它以細粒組為主，同時粘粒的存在，使其具有一定粘性。
  11. A bt - e. coli shuttle vector pht315 was deleted its replication region of bt, then constructed a novel vector named pht315 - 1 which composed a multiple cloning site, erythromycin and ampicillin - resistance marker and could only replicated in e. coli. used pht315 - 1, a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324. sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501, 333, 183aas. orfl had 98 % identities with replicating related protein ori43 of bt strain hd263. the others were no homology to any published bt replicating related protein. after continuous cultured for 70h at 30 c without antibiotic selecting press. the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 %. and growth curve also showed that the novel replicon was stable and could replicate normally

    進一步序列分析表明該復制區至少有3個較大的orf ，分別編碼501 ， 333 ， 183個氨基酸。其中orf1蛋白序列與hd263復制蛋白ori43的同源性為98 ，而另外兩個orf和genbank己公布的bt復制相關蛋白無同源性。 30連續培養72h ，復制區質粒在bt無晶體突變株hd73cry ~ -中穩定性達98以上， 30h生長曲線也表明該復制區能夠在bt中穩定復制和遺傳，對受體菌株無明顯不良影響。
  12. Drawing the following concludes : ( 1 ) for the different metallic small particles with even number or odd number, the heat capacity is varied. the result is different from the bulk metal in essence. at the low temperature, the distribution of the discrete energy levels has important effects on the heat capacity ; at the high temperature, the distribution of the discrete energy levels has little effects because of the involving of mass electrons into the energy distribution

    得到如下結論： （ 1 ）金屬小粒子所含的電子數奇偶性不同，其電子熱容是不同的，這與大塊金屬有本質區別；低溫極限時電子熱容受能級分佈的影響很大；而在高溫時，有大量的電子參與能量配位，比熱遵循大塊金屬的線性規律，能級分佈的影響小時。
  13. The vertical distribution of particle concentration is nearly linear, and the concentration at the bottom is 3 times larger than that at the top, which indicates that ca, the reference concentration of suspended particles in einstein ' s equation, is larger

    2u * ；顆粒的濃度沿垂線基本為線性分佈，底部比上部大3倍左右，說明einstein取的懸移質參考濃度ca會有所偏大。
  14. The results showed that drought stress at flowering stage delayed the date of the max filling - speed ( dmfs ), and weakened the rate of average growth ( rag ) and the max filling - speed ( mfs ), and then shorten linear dry matter accumulation phase and leveling - off of dry matter accumulation phase

    結果表明：花期乾旱導致玉米最大灌槳速度出現時間推遲、籽粒相對生長率和最大灌裝速度減弱、干物質線性積累期和干物質穩定增長期顯著縮短，乾旱結束后植株通過提高幹物質線性積累期的持續時間和干重，以彌補前期乾旱的損失。
  15. Considering the mass balance law for solute, and combining with the populance balance model and the mccabe ' s l law, a linear crystal growth rate model was presented for potassium nitrate aqueous system. the test variables of both the liquid and solid for kinetic parameter estimations were given on this model, and the experimental apparatus were also set up

    基於粒數衡算模型，以kno _ 3 - h _ 2o為模型研究體系，根據結晶過程中溶質質量守恆和mccabe定律，首先建立了晶體線性生長動力學模型，並確定出動力學參數估計所需要表徵的固液兩相物理參數，進而建立了實驗表徵方法和測定裝置。
  16. There were troubles in the continuity of the function and of its - derivative divided by band - mass on the boundary. in the theoretical calculation, the wave function is relative to the physical properties of the impurity greatly, the envelop function f ( x, y ) is expanded in terms of the one - dimensional linear harmonic oscillator function in this paper. it satisfies the continuity of the function and of its - derivative divided by the band - mass, so it improves the precision of the function and binding energy

    與以往工作不同的是，以前選用的x ， y方向電子的包絡函數f （ x ， y ）是一維有限深量子阱中波函數的乘積，在邊界上波函數的連續性和粒子流的守恆條件存在問題；而在理論計算中，波函數的選取與雜質的物理性質有密切關系，本文選取的電子的包絡函數是用一維線性諧振子的波函數展開而成的，在邊界上能夠同時滿足波函數的連續性及粒子流（ 1 / m ~ * ） f ' （ x ， y ）的守恆條件，從而使得波函數和束縛能的精確度得到了改進。
  17. Applied the above theory and the dem program trubal, the unilateral compress problems for the regular generated particles with an interstitial fluid are studied, and the macro - strain - stress curves varied with the fluid properties and the velocity of the wall are gotten. then, he unilateral compress problems for the random generated particles are numerically simulated, and the macro - strain - stress curves are obtained. moreover, the numerical simulation results for the dry and moist cases are compared

    利用上述理論以及demtrubal程序，研究了存在填隙流體時顆粒規則排列單向壓縮問題，得到了宏觀應力應變曲線隨流體性質、壁的運動速度等的變化規律；並對隨機排列單向壓縮問題進行了數值模擬，得到了宏觀應力應變平均曲線；並對干、濕兩種情況下顆粒的模擬結果進行了對比。
  18. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  19. The effective method of nucleation and growth of nanoparticles as applied to synthesize w - and mo - containing polyoxometalates nanoparticles in precursor films by making use of their acidity or oxidative property. the controllable synthesis of nanoparticles can be achieved by increasing synthetic cycle of polyoxometalates. the composition, structure, and properties of the as prepared composite films have been characterized in detail by uv - vis, ftir, x - ray photoelectron spectra, scanning electron microscopy, atomic force microscopy, transmission electron microscopy and cycle voltammograms

    利用有效的在前體膜中成核、生長納米粒子的方法，使用keggin型鎢系、鉬系多酸，有目的地利用其酸性，強氧化性，在膜中發生反應，從合成出基於多酸的納米粒子，通過增加多酸的反應循環數，我們可以實現了納米粒子的可控合成，採用uv - vis光譜、 ftir光譜、 x -射線光電子能譜、掃描電子顯微鏡、原子力顯微鏡、透射電子顯微鏡、循環伏安對所制備的納米復合膜進行了組成、結構和性質表徵。
  20. The study also shows that the increase of tio2 content lead to the ascent of particle density but had few effects on the pore structure, confirming that superfine tio2 had been successfully entrapped in the regenerated cellulose matrix

    研究表明，鈦白粉加量的增加會使基質的密度線性增加，但對基質的孔結構影響不大，說明超細顆粒已被有效地包埋進再生纖維素骨架之中。
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