繼代 的英文怎麼說

中文拼音 [jìdài]
繼代 英文
passage
  • 繼 : Ⅰ動詞（繼續; 接續; 接著） continue; succeed; follow Ⅱ副詞（繼而） then; afterwards
  • 代 : Ⅰ動詞1 （代替） take the place of; be in place of 2 （代理） act on behalf of; acting Ⅱ名詞1 （歷...
  1. Proteins associated with callus proliferation and adventitious bud differentiation of populus euphratica

    胡楊愈傷組織繼代增殖和器官發生中蛋白分子標記的研究
  2. Relative physiological and biochemical features of redifferentiation difference in three types of calli subculture in anthurium andraeanum

    花燭愈傷組織不同繼代培養的再分化差異
  3. With 24 c or 4 c, the change trends of the content of the salidroside was basically consentaneous in the consecutive cultural eras of the callus. in the callus from the different explants, the influence was maximal to the the activity of pal enzyme and the influence was lowest to the the activity of ca4h enzyme, and the influence to the the activity of tal enzyme was ascertained according to the explant of the callus. so in the same explant with the different temperature or in the different explant with the same temperature, there was no incident between the content of the salidroside and the activity of enzymes pal, ca4h and tal, and we presumed that there may be emphasized particularly on different metabolic pathway of salidroside

    （ 2 ） 、愈傷組織的外植體來源、培養溫度條件和不同的繼代培養數都影響著其中紅景天甙的含量和苯丙氨酸解氨酶（ pal ） 、肉桂酸解氨酶（ ca4h ）和酪氨酸解氨酶（ tal ）這3種酶的活性；不管是葉來源還是莖來源，不管是24培養還是4培養的愈傷組織，在連續繼代培養中紅景天甙含量的變化趨勢基本上是一致的；無論是葉來源的愈傷組織還是莖來源的愈傷組織，培養溫度對pal酶的酶活性影響最大，對ca4h酶的酶活性影響最小，對tal酶的酶活性影響視不同的外植體來源而定；在相同外植體來源的愈傷組織中及不同的培養溫度的條件下，或是在不同外植體來源的愈傷組織中及相同溫度的培養條件下，其紅景天甙含量與pal酶、 ca4h酶和tal酶的酶活性之間沒有完全一致的對應伴隨關系。
  4. The results of biological tests have demonstrated that allantoic fluid of the first passage virus did n ' t produce macroscopic pathogenic role to chicken embryos and after passaged for four times, gross lesions were observed in chicken embryo. the virus showed typical coronavirus under electron - microscope and it could n ' t form plaque in cef cells and could hemagglutinates chicken red blood cells after treatment with 1 % trypsin. to surprise, the virus replicated in cef cells also showed hemagglutination activity to chicken red blood cells. in addition, the spf chickens which inoculated with the virus isolated from the chicken damaged tissue showed clinical sign and grow lesion, but it ' s gross lesion did n ' t resemble to those of field outbreaks

    生物學特性：雞胚尿囊液經離心、磷鎢酸負染后，電鏡觀察該病毒為典型的冠狀病毒；該毒株的第一代尿囊液對雞胚無肉眼可見的致病作用，當繼代到第5代后，胚體嚴重病變；病毒在雞胚中隨著接種時間的延長，其效價增高， 96h可達到48h的2倍；該毒株可在cef上生長，但不能形成明顯的蝕斑；經1胰酶處理后可凝集雞紅細胞；雞胚的第四代尿囊液病毒回歸動物體，病死雞腎臟呈典型的花斑腎，腺胃則未見肉眼可見的病變。
  5. Carrot tissue culture and plant regeneration factors including explants, medium and culture condition are combined together to study the most efficient protocol of carrot tissue culture and plant regeneration thereof. the most suitable explant is fresh hypocotyls segment and precultured hypocotyls derived from 7 - 10 day old aseptic plantlets generating in dark or in dim light, the best recipe for cullus induction and subculture is b5c ( 85 with 0. 5mg / l 6ba and 0. 5mg / l 2, 4 - d ), the ideal recipe for plant regeneration is 65 or ms free of hormone. a phytotron with a 16 / 8 h day / night cycle, at 25 is feasible for plant regeneration, and occasional exposure to sun light dramatically stimulates plant growth

    建立了高效的胡蘿卜組織培養及再生體系以適于生產飲料的胡蘿卜「新黑田五寸人參」為材料，研究不同外植體、不同培養基，不同培養條件對胡蘿卜愈傷誘導及再生的影響，建立一套高效的胡蘿卜組織培養再生體系：最適于誘導愈傷的外植體是弱光或黑暗下發芽7 - 10d無菌苗下胚軸，最適合的愈傷誘導培養基和繼代培養是b _ 5c （ b _ 5 + 0 . 5mg l6ba + 0 . 5mg l2 ， 4 - d ） ，最適于植株再生的培養基為不添加任何激素的b _ 5或ms ，組織培養條件為25 、光照周期為16hr 8hr 。
  6. There are also different in inducing cluster buds from callus of clone gingkgo and its other tissues and organs, some callus can induce cluster buds, but others can not. differentiation rate from the callus of cotyledon can be up to 36 % and the number of cluster buds is positive related to the times of subculturing

    但是不同器官誘導的愈傷組織對于叢生芽的誘導也是不同的，子葉誘導的愈傷組織的分化率較高，有36左右，並且繼代次數的增加也能夠增加分化的幾率
  7. Many researchers have conducted experimellts about it, but not succeeded. culture media were compared to find the best medium of gingkgo culture for controlling callus browning by different sugars, antioxidants and sorbents. the result showed that the medium with ms + zt 1. 0mg / l + naa 1. 0mg / l + sucrose 50g / l + agar 8g / l + ac 2g / l was the best medium, at l5 days subculturing intervals

    銀杏組織培養過程中，尤其是在愈傷組織的繼代培養中，褐變現象特別嚴重，曾有不少的人做過這方面的研究，但都沒有成功，而本研究通過對不同糖類物質、抗氧化劑、吸附劑以及不同的培養基對褐變的影響和控制效果，探索出有效控制褐化現象發生的最佳培養條件，試驗結果表明： ms + zt1 . 0mg / l + naa1 . 0mg / l +蔗糖50g / l +瓊脂8g / l + ac2g / l培養基上的繼代效果最好，繼代時間最好在15d左右。
  8. Subculture conditions and growth characteristics of arabidopsis suspension culture

    擬南芥懸浮細胞生長特性及其繼代培養條件
  9. Pre - differentiation and proper partial desiccation of calli before transferred to regeneration medium was found to apparently improve the frequency and quality of plant differentiation. with our optimized culture condition and treatment style, induction frequency of pei ' ai64s and 9311 can be reached 62. 45 % and 85. 30 % respectively and after 3 months of subculture calli can remain high - quality embryogenic state, when high - quality embryogenic calli after two times of subculture were used as acceptor, callus differentiation frequency can arrive at 85. 5 % and 87. 7 % respectively

    採用我們優化后的培養條件與處理方式，培矮64s和9311愈傷組織的誘導頻率分別可達到62 . 45和85 . 30 ；繼代培養時間達三個月左右仍能保持較好的胚性生長狀態；對于繼代兩次胚性生長狀態良好的愈傷組織分化頻率分別可達到85 . 5和87 . 7 。
  10. The results of stem culture were as follow : b5 medium had faster bud germination and low browning. when subculture medium was b5 + ba2mg / l + naa0. 2mg / l, the numbers of propagation were 3. 2 times of explants, what ' s more, the seedlings were robust

    蝟實繼代培養基b _ 5 + ba2mg l + naa0 . 2mg l最為有利於蝟實芽的增殖，其芽的增殖數為接種數的3 . 2倍，小苗生長健壯。
  11. Study on the suitable conditions for shoot propagation and rhizogenesis of robinia pseudoacacia cv idaho

    香花槐試管苗的繼代培養和生根移栽條件研究
  12. Discussion of the subculture of parent eggs from the perspective of the abnormalities in the grandparent eggs of f

    從原原種中的異常現象看母種繼代
  13. The optimum concentration of kanamycin was different at different stage of culture, which was 50mg / l at calli induction stage, 100mg / l at embryogenic calli selecting stage, and 50mg / l at plantlet root elongating stage, respectively

    把愈傷組織誘導階段的kan濃度定為somg / l ;愈傷組織繼代的kan濃度定為100m叭;小植株長根的培養基的kan濃度定為som叭。
  14. The main results are as follows : ( 1 ) plant regeneration system of tall fescue embryogenic calli was established according to studies on impacts of manifold factors on callus induction from mature seeds and subculture production and differentiation of embryogenic calli

    主要研究結果如下： （ 1 ）通過對成熟種子愈傷組織誘導、胚性愈傷組織繼代發生和分化的多種影響因素的研究，建立了高羊茅胚性愈傷組織植株再生體系。
  15. At primary culture, pgcs co - cultured with their gonadal stromall cells were well grown. when subculture, we used primary chicken embryonic fibroblast ( pcef ), primary mice embryonic fibroblast ( pmef ) and snl cells to make feeder cells. forward research founded that the pcef cells were the most suitable for the growth of putative eg cells when having various cytokines

    繼代培養時，經過反復實驗比較雞胚原代成纖維細胞飼養層（ pcef ） 、小鼠原代成纖維細胞飼養層（ pmef ） 、 snl細胞飼養層等三種不同飼養層的作用效果，最終發現以雞胚原代成纖維細胞製作飼養層同時添加各種生長因子最適合雞類eg細胞的生長，但pcef與pmef之間的差異不顯著。
  16. 2. the resistence of transformators were selected by g418 after co - culture with agrobactrium tumeflien, which was 40 mg / l in subcultured medium and 50mg / l in differeniated medium. in the process of killing agrobactrium, cef, amp or carb were also useful and the time of inhibition was long too

    經與農桿菌共培養后轉化子的鑒定選用g418 ，在繼代篩選培養基中添加40mg l ，在分化篩選培養基中添加50mg l ；在除菌過程中，頭孢噻肟鈉、羧芐青霉素、氨芐青霉素抑菌效果較好，抑菌時間較長。
  17. It was also noted that irradiation treatment with low dose ( 10gy ) of y rays exerted slight stimulating effects on callus induction and formation of embryogenic calli in particular. production of embryogenic calli was obviously promoted by addition of 0. 1 ~ 0. 2mg / l bap or 2. 5mg / l cuso4 5h2o, or enhancement of sucrose concentration to 60g / l in subculture medium

    在繼代培養基中添加0 . 1 0 . 2mg lbap或2 . 5mg l硫酸銅，或將繼代培養基中蔗糖濃度提高到60g l能促進胚性愈傷組織發生，提高胚性愈傷組織頻率。
  18. Four green plants were regenerated form resistant calli of wen. 6 derived form 1500 implanted embryos. no green plant was regenerated form calli of 200 non - transformed embryos. pcr assays of 4 green plants showed that two of them attained the expected size of amplified dna fragment ( 1500bp )

    在含有20 - 60mg / l潮黴素的ms培養基上經誘導和繼代培養后，獲得了一批抗性愈傷組織，經過分化培養獲得4株再生苗，對照的200枚幼胚未獲得再生苗，對再生苗進行pcr檢測。
  19. Study on influence factors to dormancy during enrichment culture of double petals common sinningia speciosa benth. et hook

    重瓣大巖桐繼代過程中休眠現象的影響因素研究
  20. After 4 times selected culture by ppt, 347 resistant calli were obtained. the ppt resistant calli were transferred to ms medium with 2. 0 mg / l 6 - ba, 0. 1 mg / l naa for regeneration

    0mg l +蔗糖30g l的選擇培養基中進行選擇培養，每10天繼代1次，連續選擇4次，共得到347塊抗性愈傷組織。
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