肽片段 的英文怎麼說

中文拼音 [tàipiānduàn]
肽片段 英文
peptide fragment
  • 肽 : 名詞[化學] （有機化合物） peptide
  • 片 : 片構詞成分。
  • 段 : Ⅰ量詞（部分） section; segment; part; paragraph; passage Ⅱ名詞（姓氏） a surname
  • 片段 : part; passage; fragment; extract; segment; bit; episode; snatch; section
  1. Main methods and results are as followed : 1 epitope analysis of agonist - binding region of nrla physicochemical properties and antigenicity of two agonist - binding regions of nrla were analyzed through bioinformatics : domain p1 containing 151 amino acid residues preceding the first transmembrane domain of the human nrla, domain p2 with 144 residues following the third transmembrane domain. four parameters including hopp - woods and kyte hydrophilicityjanin accessibility, karplus - schulz flexibility, and welling antigenicity were used to determine the antigenic sites, and prosite programme and chou - fasman method were employed to analyze their related sequence motif and the secondary structures

    用goldkey軟體分別選取公認的hopp等與kyte等親水性參數、 jain表面可及性參數、 karplus - schulz主鏈柔韌性參數及welling抗原性參數對p1 、 p2兩個多肽片段進行參數分析。並採用通用的prosite程序與chou - fasman方法比較分析p1 、 p2多肽片段的氨基酸位點與二級結構特徵。綜合判定兩個多肽片段的抗原性及其位點，結果認為p2抗原性強于p1 。
  2. Five analogues and five segments were designed and synthesized by using solid phase synthesis method according to separated papaver somniferum pollen tridecapeptide with antitumor activities as leading peptide. their primary secondary structures in solution were determined by cd spectra and their inhibitive activities to human liver and mammary gland cancer cells were assayed by mtt method. the relationship of structure - activity was studied and discussed

    罌粟花粉十三肽對人肝癌和人乳腺癌腫瘤細胞具有明顯的抑制作用，以其為先導化合物，設計併合成了5個類似物和5個片段，結合cd譜測定的二級結構及它們對人肝癌和人乳腺癌腫瘤細胞的抑制作用測定結果，研究並討論了該肽結構與抗腫瘤活性的關系
  3. Proteins were identified with peptide mass fingerprinting using matrix - assisted laser desorption ionization time of flight mass spectrometry ( maldi - tof ms ) after tryptic in - gel digestion

    差異蛋白經胰蛋白酶酶切后產生肽片段，再利用基質輔助激光解吸電離飛行時間質譜得到肽指紋圖譜來鑒定。
  4. A approximately 460bp dna fragment was amplified by pcr from lactococcus lactis nizo r5 genome. the primers used in this study comprised the following nucleotide sequences : 5 " - cgcgaattcgatataggtttattgagt - 3 " and 5 " - atgaagcttatccatgtcagaactaa - 3 ". conditions used for the pcr consisted of 30 cycles of 94 ? for0. 5min, 45 ? for imin and 72 ? for 1. 5min, plus one additional cycle of 72 for 10min

    根據已發表的乳鏈菌肽前體基因的dna序列，設計兩條引物並引入酶切位點。以lactococcuslactisr5基因組dna為模板， pcr反應條件： 94變性30s ， 45退火60s ， 72延伸90s ，反應進行30個循環。成功擴出一條約460bp的dna片段。
  5. These findings of the specific ctls epitopes in irbp and pedf may lead to improved understanding of the pathogenesis of uveitis. our study also provides a strategy to identify specific ctls epitopes within tumour antigen, which is helpful to make tumour vaccine for the patients

    本研究也為尋找腫瘤抗原中具有誘導特異性ctls能力的肽片段提供了一種策略，為制備肽片段腫瘤疫苗提供了理論基礎。
  6. In this article, the author amplified dna sequences encoding the mature peptides of human intact and truncated igf - 1 gene from human genomic dna by the new way of alternative pcr established and named as exons - piecing together method by this laboratory. the igf - 1 and des ( l - 3 ) igf - l gene were cloned in pgem - t vector and then subcloned in expression plasmid pgex - 3x. two recombinant constructs known as pgex - 3x - igf - l and pgex - 3x - des ( l - 3 ) igf - l were transformed to e. coli dh5 a respectively

    我們利用外顯子拼接法從人的基因組dna中擴增了編碼完整型及截短型人igf - 1基因成熟肽的dna片段，並將其克隆至pgem - t載體中；經測序證明正確后，又構建了表達載體pgex - 3x - igf - 1及pgex - 3x - des （ 1 - 3 ） igf - 1 ，在大腸桿菌中進行了表達研究。
  7. The gene of amoa in ammonia - oxidizing encodes the active - site polypeptide of ammonia monooxygenase which catalyzes the oxidation of ammonia to hydroxylamine. we designed a pair of primers special for the amoa gene by comparing the known amoa gene sequences and used pcr to amplify the amoa gene fragments

    Amoa基因是編碼氨單加氧酶活性多肽位點基因，我們通過引物篩選合成了對氨氧化細菌amoa基因特異結合的引物序列，利用pcr技術對活性污泥中的amoa基因片段進行特異擴增，得到的dna片段大約為490bp 。
  8. 57 protein spots out of about 1000 detectable spots on the 2 - d gels were indentified by the following two methods : l ) n ~ terminal edman degradation microsequencing after the protein spots were electro - transferred to pvdf membrane. 2 ) maldi - tof - ms peptide fingerprint analysis of the protein spots and protein database searching. the 2d protein maps of the rice spikelet during the sterile and fertile stages were compared

    二是通過原位酶解，抽提蛋白質片段進行maldi - tof質譜分析，利用肽質量指紋圖數據在數據庫中進行檢索，在雙向電泳凝膠上取了57個點進行分析，有34個蛋白質點在數據庫得到歸屬鑒定。
  9. The result of sequencing shows that this fragment contains an open reading frame of 1106 nucleotides and encodes 369 amino acids residues, which is part of a alkaline serine protease, with 98. 3 % homogeneity to a reported gene from bacillus pumilus

    序列分析顯示，該片段是一個全長1106bp的orf ，可編碼369個氨基酸殘基的多肽，與短小芽孢桿菌堿性蛋白酶基因編碼區高度同源。
  10. C57bl / 6 ( h - 2b background ) mice were subcutaneously immunized with selected peptides in ifa ( incomplete freund ' s adjuvant ) respectively. 8 days later after immunization, spleens were removed to prepare splenocytes suspension

    將這些肽片段分別與不完全弗氏佐劑制備成乳濁液，皮下注射免疫c57bl / 6小鼠， 8天後，處死小鼠，取出脾臟制備脾細胞懸液。
  11. In he - 3 and de - 3, 16 protein spots that were absolutely different ( only expressed in diploid embryos but not in haploid embryos or vice versa ) and 16 protein spots that were up - and down - r gulated were identified unambiguously. in he - 2 and de - 2, 20 protein spots that were absolutely different ( only expressed in diploid embryos but not in haploid embryos or vice versa ) and 5 protein spots that were up - and down - regulated were identified unambiguously, some of these different proteins are correlative with eyes development, nerve development, development regulation, cell differentiation and maintenance, arthromere formation and signal transduction. it shown that gene expression have significant difference between diploid and haploid during embryos developing

    分別選取其中70個分辨好的差異蛋白質點進行原位胰蛋白酶酶解，酶解的肽片段用基質輔助激光解析電離分行時間質譜（ maldi - tofms ）得到肽質指紋圖譜，再在網上peptident軟體中搜索swiss - prot和trembl數據庫，初步鑒定了he - 3和de - 3上32個差異蛋白質點，其中包括16個表達量上有差異的蛋白質點（ p 0 . 05 ）和16個有無表達的差異蛋白質點，並且初步鑒定了he - 2和de - 2上25個差異蛋白質點，其中包括5個表達量上有差異的蛋白質點（ p 0 . 05 ）和20個有無表達的差異蛋白質點。
  12. The full - length sequence, the 3 ' - deletion fragment, the sequence encoding the mature protein and the sequence encoding the conservative domain, were cloned using synthesized primers. recombinant expression vectors were constructed through directional cloning and then host e. coli were transformed by the vectors

    設計特異引物克隆得到毒蛋白基因的4個片段，即基因全長、末端缺失片段、編碼成熟肽的片段及編碼活性區域的片段。
  13. The former expresses recombinant proteins with a 6xhis tag at n - terminal. the fore mentioned four fragments were all used for expression in this system and the mature protein and the conservative domain were effectively expressed while the expression of the other two was unobvious

    前者表達n ?末端加6 his標記的融合蛋白，克隆到的4個基因片段均進行了表達，其中成熟肽和活性區域得到了大量表達，蛋白全長和末端缺失片段表達不明顯。
  14. The human total rna was extracted from the foetal liver tissue sample of spontaneous abortion. mrna was separated and purified from total rna. apoe3 cdna was amplified by rt - pcr with a signal or without a signal sequence

    從正常流產胎幾肝組織中提取總rna ，分離純化mrna ，以mrna為模板， rt pcr特異擴增帶信號肽和不帶信號肽的人apoescdna片段，將apoe
  15. The maldi peaks corresponding to the c - terminal peptide fragments of proteins were specifically enhanced, discriminating against those from internal peptides that were not tagged with a positive charge

    與那些沒有正電荷標記的中間肽段的maldi譜圖峰明顯不同的是，蛋白質c端肽片段的maldi譜圖峰明顯增強。
  16. Angiostatin ( as ) is a kind of polypeptide identified as a product of plasminogen cleavaged by protease. it blocks neovascularization by directly inhibiting endothelial cell proliferation and migration

    血管抑素（ angiostatin ， as ）是血漿纖溶酶原（ plasminogen ， plg ）經蛋白水解酶降解生成的多肽片段，是直接作用於血管內皮細胞的血管生成抑制劑。
  17. The study of in - situ construction of cytocompatible surface on pdl - la matrix via amphiphile - amino acid ( rgd ) hybrid self - segregation - the amphiphilic diblock copolymer, poly ( dl - lactide ) - poly ( ethylene oxide ) ( pla - peo ) copolymer, containing hydrophobic pla block and hydrophilic peo block was synthesized via coupling method in this dissertation. cell - adhesion - promoting amino acids and integrin receptor peptide rgd were then immobilized at the end of peo chain of pla - peo copolymer via hydroxyl group activation technique. the solvent blending and casting method was then used to obtain the amphiphile modified pdl - la membranes

    兩親共聚物-氨基酸（ rgd ）雜化體原位自修飾構建聚乳酸細胞相容性表面的研究一本論文首先設計併合成了一類含疏水聚乳酸（ pla ）鏈段和親水聚氧乙烯（ peo ）鏈段的兩親嵌段共聚物材料（ pla - peo ） ，利用peo鏈端的活性官能團羥基固定了促細胞粘附的氨基酸及整合素配體多肽片段rgd 。
  18. In the research ts containing antigenic sites b and c was expressed with high efficiency

    本研究高效表達了tgev含有b和c抗原位點的多肽片段（ ts ） 。
  19. Based on the " self - migrating and surface - segregation " behavior, the amphiphile segregation surface was therefore constructed on hydrophobic pdl - la matrix, which containing the structure of peo spacer combining cell adhesion ligands to mimic the extracellular matrix ( ecm )

    基於兩親共聚物在疏水高聚物材料內的自遷移和表面富集行為，在聚乳酸材料上構建了以peo橋聯氨基酸或整合素配體多肽片段的類細胞外基質表面。
  20. While there are some difference in amino acid with other group ( except tw 95 ), the amino acid in 65 - 75 of hn of ndv group are conserved, while he in 81 is conserved. based on epitope prediction, five pairs primers are designed with enzyme sites. five segments of f1, f2, f3, f4 and hn1 coding epitope was amplified and cloned to prokaryotic expression vector, respectively

    在表位預測的基礎上，將臨近的表位合併，設計引物並引入酶切位點，以fa 、 fb 、 hna為模板擴增含抗原表位的多肽片段f1 、 f2 、 f3 、 f4 、 hn1 ，克隆到原核表達載體ppro ~ （ ex ） ht上，成功地構建了五個表達載體。
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