肽間插序列 的英文怎麼說
中文拼音 [tàijiānchāxùliè]
肽間插序列
英文
peptide intervening sequence-
A gus report gene, gained by the method of pcr, was inserted into the pet22b vector between the sites of hindiii and xho i to construct a transitional vector named pet22g then the signal peptide ( pelb leader ) in the pet22g vector was replaced by a containing grb - ast signal peptide fragment to construct expression vector pet22asg which had been transformed into expression host e. coli bl21 ( de3 ) plyss
先將pcr獲得的gus報告基因插入到經hind和xho雙酶切的pet22b中,構建了中間載體pet22g ;然後再將pcr克隆的一含ast信號肽的片段as取代pet22g上的信號肽pelbleader序列,構建了大腸桿菌分泌表達載體pet22asg 。
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