胍離子 的英文怎麼說
中文拼音 [guālízi]
胍離子
英文
guanidinium ion-
The effects of various mutagenic agents on the productivity of avermectin by slreplomyces avermilitis were evaluated in this work. three different mutagenic breeding methods were developed, such as ntg, uv, ion implatation
在育種研究中應用了離子注入、亞硝基胍和紫外線誘變方法，並對這幾種誘變方法進行了比較，發現離子注入的誘變效果最好。The total rna was purified from the germ in the liquid by the guanidine isothiocyantehod method, then the total rna digested by dnase that had not rnase was used for rt - pcr. i change the magnesium ion dencity in the pcr system in order to optimize the pcr condition. at the end i selected the magnesium ion density as 1. 25 mm. the production of rt - pcr was inserted directionally into pgem ? z ( ampr ). the pgem ? z ( ampr ) was used to transform e coli jm109. i got a positive clone through culling and identificatin. the dna sequence inserted into pgem ? z ( ampr ) was sequenced and blasted with the cdna sequence of the # - mannanase mature peptide that got from genbank
分取誘導培養液中的菌體，用異硫氰酸胍法提取總rna ，總rna再經無rna酶的dna酶處理後用于rt ? pcr 。在pcr擴增目的基因時，通過優選擴增體系，使鎂離子濃度為1 . 25mm時rt ? pcr可順利地獲得目的基因，並能定向克隆到載體pgem ? 3z （ amp ~ r ）中。用克隆載體轉化宿主大腸桿菌jm109 ，通過篩選獲取陽性克隆子，對陽性克隆子進行酶切與pcr鑒定，並對載體中插入的目的基因進行測序。Guanidinium ionic liquids are concerned mainly due to their high thermal stability, chemical stability and catalytic activity
摘要胍鹽離子液體由於具有較高的熱穩定性、化學穩定性和催化活性而受到關注。
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