The results of biological tests have demonstrated that allantoic fluid of the first passage virus did n ' t produce macroscopic pathogenic role to chicken embryos and after passaged for four times, gross lesions were observed in chicken embryo. the virus showed typical coronavirus under electron - microscope and it could n ' t form plaque in cef cells and could hemagglutinates chicken red blood cells after treatment with 1 % trypsin. to surprise, the virus replicated in cef cells also showed hemagglutination activity to chicken red blood cells. in addition, the spf chickens which inoculated with the virus isolated from the chicken damaged tissue showed clinical sign and grow lesion, but it ' s gross lesion did n ' t resemble to those of field outbreaks
生物學特性:雞
胚尿囊液經離心、磷鎢酸負染后,電鏡觀察該病毒為典型的
冠狀病毒;該毒株的第一代尿囊液對雞
胚無肉眼可見的致病作用,當繼代到第5代后,
胚體嚴重病變;病毒在雞
胚中隨著接種時間的延長,其效價增高, 96h可達到48h的2倍;該毒株可在cef上生長,但不能形成明顯的蝕斑;經1胰酶處理后可凝集雞紅細胞;雞
胚的第四代尿囊液病毒回歸動物體,病死雞腎臟呈典型的花斑腎,腺胃則未見肉眼可見的病變。
The grinding machine grinds some facets of both front side and backside of the glassbeads, using the special roller type grind wheel. then polish the glass beads by polishing machine
用特製的滾筒式磨輪,將水鉆毛
胚前後及
冠頂面打磨成多個面,然後進行拋光。
The grinding machine grinds some facets of both front side and backside of the glass beads, using the special roller type grind wheel. then polish the glass beads by polishing machine
用特製的平面式磨輪,將水鉆毛
胚前後及
冠頂面打磨成多個面,然後進行拋光。
In order to understand the mechanism of settlement and metamorphosis in styela canopus primarily, experiments observated the embryo stage of s. canopus and studied some chemical and biological factors which affect the settlement and metamorphosis of s. canopus larvae
在實驗室條件下,對
冠瘤海鞘( styelacanopus )的
胚胎發育階段進行了觀察,研究了影響其附著變態的一些化學和生物因子,初步解釋
冠瘤海鞘幼體附著變態的機制。
Expression analysis the expression analysis of gharf in different tissues of cotton was carried out by rna dot blotting with ghakf probe labeled with dig. the result showed that the expression of arf gene was mainly in anthers of sterile and fertile, pollen of fertile, corolla rather than in leaves, radicel and ovule
棉花ari ; 』基因的表達分析用地高辛將棉花arf基因標記為探針,與棉花洞a的不育株花藥、司有株花藥、可育花粉粒、葉片、花
冠、
胚根和
胚珠的總rna進行斑點雜交。
All the 4 extractions showed obvious pharmacological effects by decreasing the area of myocardial infraction of the model rats, increasing the amount of blood stream of the isolated guinea - pig hearts and reducing the beating rate and amplitude of cultured myocardial sheets of mice
方法:採用
冠狀動脈結扎致大鼠急性心肌缺血模型,觀察革葉獼猴桃果實70 %乙醇提取物、正己烷部位、氯仿部位及正丁醇部位對急性心肌梗塞范圍的影響,同時觀察上述部位對豚鼠離體心臟
冠脈流量及小鼠
胚胎心肌培養細胞功能的影響。
Inhibitory effect of ifn - on tumor - induced angiogenesis
部分
冠心病常用中藥對雞
胚尿囊膜的促血管生成作用研究
A study on efficcient somatic embryogenesis and plant regeneration in crownvetch
小冠花高效體細胞胚胎發生與植株再生的研究