胞質基因 的英文怎麼說
中文拼音 [bāozhíjīyīn]
胞質基因
英文
cystomyxo-adenoma genes-
First, to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp, and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second, based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins, the concentration of the hab toxins could be detected
目的:構建一個含c - fos啟動子和egfp報告基因的pegfp - c - fos重組質粒載體。體外轉染膀胱癌biu - 87細胞后,利用赤潮毒素作用后細胞表達綠色熒光蛋白的變化來檢測赤潮毒素,初步建立一種以細胞為基礎受體水平的赤潮毒素檢測方法。The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中,通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中,然後採用葉盤法,在該農桿菌的介導下將pap基因導入普通煙草中,經過卡那黴素抗性篩選,最後獲得了轉pap基因的工程煙草植株,摩擦接種煙草花葉病毒( tmv ) ,與非轉基因煙草相比,能夠推遲癥狀表現達2月之久,說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。This paper introduced the application of biotechnology in rice genetics and breeding, including tissue culture, cell mutants selection, protoplast fusion, apomixis, molecular mark assisted breeding and genic transformation
簡要綜述了生物技術在水稻遺傳育種中的應用,主要包括組織培養、細胞突變體的篩選、原生質體融合、無融合生殖以及分子標記輔助育種和轉基因技術等方面。Methods : an artificial gene for fl extracellular domain cdna was synthesized by using favored genetic codons of pichia pastroris. by inserting human fl extracellular domain cdna coding 156 amino acid resi dues into pichia pastoris expression vector ppic9k containing aox1 promoter and the sequences of alpha secreting signal peptides, a recombinant expression plasmid ppic9k - fl was constructed, and integrated into the alcohol oxidase region of the host genome
為了提高外源基因的表達量,我們根據畢赤氏酵母偏愛密碼子人工合成了編碼fl胞外區156個氨基酸的cdna序列,目的序列被定向克隆到酵母分泌型表達載體ppic9k質粒上,構建ppic9k - fl表達質粒。Primary analysis of differential gene expression profile of type 1 and type 2 astrocytes
兩型星形膠質細胞基因表達譜差異的初步觀察These confirmed the successful transformation of the a78 - 3 and a78 - 4 on selection medium containing basta. microarray on membranes were fabricated from a set of 384 pinus taeda genes ( cdnas ) related to lignin synthesis, adaptation or primary metabolism for examination of gene expression in the sublines. the results showed : ( 1 ) the correlation coefficients between the transgenic sublines a78 - 3 and a78 - 4 and the untransformed control a95 : 88 : 22 were 0. 8028 - 0. 9028, while those of a78 - 5 are 0. 8897 - 0. 9302
選擇384個與木質素生物合成及植物生理代謝和環境適應性有關的基因或cdna片段構建尼龍晶元膜,並對轉基因細胞亞系a78 - 3 、 a78 - 4和a78 - 5和對照亞系a95 : 88 : 22等培養再生植株進行基因表達的微陣列檢測,結果表明: ( 1 )三個亞系與對照之間的pearson相關系數分別為0 . 8607 、 0 . 7975和0 . 9630 。Among the genes, there were genes directly related to liver regeneration : fetuin, cathepsin ; close related to liver function : cytoplamic aspartate aminotranferase, gutathion sulfur transferase ; related to substance and energy metabolism : atp synthetase, ribosomal protein, and related to stress response : haptoglobin, transferrin
這些基因中有和肝再生有直接關系的如:胎球蛋白、組織蛋白酶;和肝臟功能密切相關的如:胞質天冬氨酸轉氨酶、谷胱甘肽硫轉移酶;與物質能量代謝有關的如: atp合成酶、核糖體蛋白;以及與急相反應有關的如:觸珠蛋白、轉鐵蛋白。The development of a multicellular organism results from the selective expression of genes and the maturation of proteins is dependent on molecular chaperons
多細胞生物體發育的實質是基因的選擇表達性,表達蛋白的成熟則有賴于分子伴侶的協助。We showed here that axudl mrna expression was elevated by tgf - 1 treatment in a time and dose - dependent manner in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and also indicated that de novo protein synthesis was required for this response using the protein synthesis inhibitor cycloheximide, suggesting that axudl is a primary target of tgf - signalling
在hepg2和spc - a1細胞中, tgf - 1能明顯誘導這兩種細胞中的axud1基因的表達,而且表現出時間和濃度的依賴效應。同時,利用第一軍醫大學博士學位論文蛋白質合成的特異抑制劑還發現, tgf一pl誘導的axudl基因的表達過程需要新的蛋白質的合成,提示axudl基因是tgf一p信號通路的一個主要靶分子。By tagging the oxt gene with a gfp tag, it was found that this gene mainly expressed in the cytosol
通過把。 xt基因加上gfp標記,發現該基因主要在細胞質中表達。The majority of mitochondrial proteins are encoded by nuclear genes, synthesized in the cytosol and subsequently imported into mitochondria
摘要線粒體的大多數蛋白質是由核基因編碼、細胞質合成,而最終運輸到線粒體。The study results of this paper can serve the two scientific subjects of our teaching and research group as basic data calculation and elementary exploration. the two subjects are : constructing high activity - amylase genes by dna shuffling technology and studying on the evolution in vitro by mutational pcr ( with 5 - br - dutp as substitute partly ) and dna shuffling technology. - amylase ( ec 3. 2. 1. 1 ; 1, 4 - a - d - glucanohydrolase ) can catalyzes the hydrolysis of - 1, 4 - glycosidic bonds of starch from middle and liberates - maltose, - glucose and - limit dextrin stepwise
本試驗根據genbank已公布的黃單胞菌-澱粉酶基因的核苷酸序列由引物設計軟體premierprimer5 . 0輔助設計了一對引物( primer & primer ) ,以pbluescript ks +和puc18 / puc19質粒為載體,用常規的pcr方法從xanthomonascampestrispv . malvacearum ( smith ) dye等七株黃單胞菌( xanthomomasspp . )的基因組dna中克隆得到8個基因片段,分別命名為zhyf001 zhyf008 。The astrocyte marker gfap and oligodendrocyte marker mbp were not detected. nor the endoderm and mesoderm markers
膠質細胞標志基因gfap 、 mbp未檢測到,其他胚層的標志性基因也沒有表達。Roger kornberg won for his work illuminating the process in which genetic information in cells is translated into the proteins that control cellular structure and function
羅傑孔柏格則因其研究說明細胞的基因訊息如何轉錄到控制細胞結構和功能的蛋白質的過程而得獎。Gene therapy based on mesenchymal stem cells
間質幹細胞的基因治療Green fluorescent protein is widely applied in researches of modern life science, such as gene product moved - process in vivo, protein localization, drug screening and preliminary selection of transgenic individual as a molecular marker
綠色熒光蛋白這種獨特的生物學特性,使其作為報道基因在現代生命科學研究領域中,如細胞內基因產物的動態過程,蛋白質在細胞內的定位,藥物的篩選,以及轉基因個體的初步鑒定等等有著廣泛的應用。3. to study the expression of egfr and egfrmrna in jar choriocarcinoma cells and to explore the effect of egfr on the pathogenesis of choriocarcinoma
研究緘毛膜癌j皿細胞egfr基因和蛋白質的表達,與正常孕早期滋養層細胞比較,探討egfr與絨毛膜癌發病的關系。A new report says human embryonic stem cells kept for long periods of time develop changes in their genetic material
一份新報告指出,長期保存的人類胎兒幹細胞,其基因物質會逐漸產生變化。Those responsible for basic cellular housekeeping functions, such as the genes in the ribosomal protein family, are abundantly expressed, providing more opportunities to create processed pseudogenes
那些負責細胞基本功能的基因(像是核糖體蛋白質基因)活動頻繁,有比較多的機會產生加工型偽基因。Gene expression detection during neural cell differentiation of rat mesenchymal stem cells induced by astragale with gene chip
黃芪誘導大鼠骨髓間充質幹細胞分化為神經樣細胞的基因表達譜分享友人