胞質變動 的英文怎麼說
中文拼音 [bāozhíbiàndòng]
胞質變動
英文
cytocinesis-
First, to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp, and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second, based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins, the concentration of the hab toxins could be detected
目的:構建一個含c - fos啟動子和egfp報告基因的pegfp - c - fos重組質粒載體。體外轉染膀胱癌biu - 87細胞后,利用赤潮毒素作用后細胞表達綠色熒光蛋白的變化來檢測赤潮毒素,初步建立一種以細胞為基礎受體水平的赤潮毒素檢測方法。The result shows that the life cycle of all the species comprises a unicellular amoeboid or swarm cell stage, a multinucleate plasmodium stage and a sporulation stage
結果表明,生活循環均包括單核的黏變形體或游動胞、多核的營養體原質團以及形成孢子階段。Glial cell line - derived neurotrophic factor ( gdnf ) was originally isolated based on its potent and specific ability to promote the survival and morphological differentiation of dopaminergic neurons and motoneurons in embryonic midbrain cultures. in addition, gdnf also support the survival and regulate the differentiation of many peripheral neurons, including sympathetic, parasympathetic, sensory and enteric neurons. gdnf also plays a crucial role outside the nervous system, as a morphogenetic factor in kidney development and as a regulator of spermatogonia differentiation
Gfr 1與gdnf結合位點的研究膠質細胞源性神經營養因子( gdnf )對多巴胺能神經元、運動神經元、感覺神經元、腸道神經元等多種神經元具有促進存活及保護作用,它還能促進腎臟的發育和精原細胞的成熟,因此,極有希望用於治療神經損傷和神經系統退行性病變。This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected
本研究以蛋白質分子設計的理論和方法研究避孕疫苗,將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合,獲得新抗原- 35肽序列;並在合成、純化後分別與弗氏佐劑、免疫刺激復合物( iscoms )混合后免疫不同遺傳背景的雌性小鼠,觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變:以及在ivf下,新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。The seven sisters that dr de grey wishes to slaughter with sens are cell loss, apoptosis - resistance ( the tendency of cells to refuse to die when they are supposed to ), gene mutations in the cell nucleus, gene mutations in the mitochondria ( the cell ' s power - packs ), the accumulation of junk inside cells, the accumulation of junk outside cells and the accumulation of inappropriate chemical links in the material that supports cells
德?戈瑞博士想用這一策略對付的七個因素是:細胞丟失,細胞凋亡阻抑(細胞拒絕程序死亡的傾向) ,細胞核內的基因突變,線粒體(細胞的「動力倉」 )中的基因突變,細胞內的垃圾堆積,細胞外的垃圾堆積和細胞支持性物質中不當化學鍵堆積。A temporary fingerlike projection or lobe on the body of an ameboid cell. it is formed by a flowing action of the cytoplasm and functions in locomotion and feeding
指某變形蟲細胞表面臨時形成的指狀突起或裂片。通過細胞質流動所形成,以完成運動和取食功能。To get in vivo evidences that apoplast calmodulin con 1d regulate plant growth and development process, a chimeric secretion form of calmodulin binding peptide, which contains a signal peptide, a calmodulin binding domain and a c - myc epitope was constructed. the chimeric gene was introduced into arabidopsis. it was expected that the overexpression of this chimeric protein could be secreted into cell wall and bound to apoplast calmodulin, which could reduce the apoplast calmoduin concentration to make an apoplast camodulin " antisense " plant. by observing the potential phenotype change of apoplast calmodulin " antisense " plant, the in vivo function of apoplast calmodulin on plant growth and developmental process could be speculated
但這些多是採用生理學手段和藥理學方法而得出的體外( invitro )實驗結果,為了取得質外體cam在植物生長發育過程中發揮重要作用的invivo實驗證據,根據動物中的一些研究方法,本實驗設計並構建了帶有信號肽、 cam結合肽( can小肽) 、 epitope ( c - myc )融合基因的載體,並將融合基因通過真空滲入法轉入擬南芥,預期過表達的融合蛋白將會被分泌到細胞外並與質外體cam相結合,這樣就會抑制質外體cam的功能,從而可以構建質外體cam的「反義」植株,通過觀察質外體cam 「反義植株」的表型改變,就可以推斷質外體cam在植物生長發育過程中的功能。The quality of feeder layer is affected by a lot of factors, such as animal breed, culture medium, passages in vitro and experiment condition, etc. as to the production of feeder layer, there are a few reports about morphological and histologic change when of embryonic body fibroblast when culturing in vitro and cryopreservation, so kunming mouse were chosen as experimental animals and morphological and histologic changes were studied in course of its embryonic body culturing. we expect to offer theoretical foundation to our laboratory for setting up feeder layer storehouse. at the same time, the feasibility of myocardium tissue culturing with fibroblast layer altogether was studied so that established foundation for studied the biological characteristic of heart outside body
小鼠胚體成纖維細胞的培養是制備飼養層的重要途徑,其制備、傳代及冷凍保存均有不同的研究報道,飼養層的質量受許多因素的影響,如動物的品種、培養液、所傳代數及實驗條件等,關于飼養層制備過程中的胚體細胞培養、傳代、冷凍后的細胞形態、組織學等方面的研究報道很少,故本實驗以昆明小白鼠為實驗動物,研究其胚體培養過程中細胞的形態學、組織學等方面的變化,以期為本實驗室建立飼養層細胞庫提供理論依據,同時探討心肌細胞和成纖維細胞層共培養的可行性,以期為心臟生物學特性的體外研究奠定基礎。Along with the development of the cytobiology and the molecular biology, and thoroughly research of the biophysics, the biochemistry, the genetics and immunology, it has cultivated the modem biological technology, such al genetic engineering, cellular engineering, enzyme engineering, fermentation engineering and so on, to change biology characteristic to carry on the material transformation, has formed the front biological examination technology : the dna probe, the pcr technology, the molecular mark, the bioluminescence technology, genechip technology and so on the widespread application of these advanced biotechnologies in dairy industry baa impelled the dairying technical transformation, and has been having vital significance to dairy production, research and dairy product security
摘要隨著細胞生物學和分子生物學的發展及對生物物理、生物化學、遺傳學和免疫學研究的深入,培育了基因工程、細胞工程、酶工程、發酵工程等改變生物特性進行物質轉化的現代生物技術,形成了dna探針、 pcr技術、分子標記、生物熒光技術、基因晶元技術等前沿性的生物檢測技術,其在乳品工業中的廣泛應用,推動了乳業的技術變革,對乳品生產、研究和乳品安全意義重大。Objective to investigate the mechanism of ear acupuncture by studying the adjustment of ear acupuncture on activity of the nucleus paragigantocellularis lateralis of rabbit
目的觀察耳針對家兔旁巨細胞外側核( pgl )活動改變的影響,嘗試從遞質水平上探討耳針機制。Under high osmotic shock, the size of the cells obviously shrink, so did the chloroplasts, mitochondria and golgi bodies, etc. the membranes of these organelles partially melt with that of endoplasmic reticulum, which makes its surface area increase
在高滲震動下,細胞體積明顯變小,胞內細胞器如葉綠體、線立體、高爾基體等的體積也相應變小,這些細胞器的膜部分融合至內質網膜中,使內質網的表面積增大。In our laboratory, a unique mutation detection system using a shuttle vector plasmid has been established to demonstrate that a low concentration of mnng ( 0. 2 m ) can induce nontargeted mutation in mammalian cells : the mammalian cells were exposed to 0. 2m mnng for 2. 5h, then a shuttle plasmid pz189 carrying supf trna gene was transfected into cells after 24h culture. we found a 5 - fold higher mutation frequency of the plasmid replicated in pretreated cells than the spontaneous mutation frequency of the plasmid replicated in control cells. this kind of mutation did not occur immediately after mnng exposure
我們實驗室曾用一特殊的突變檢測系統,直接證明dna損傷劑可在哺乳動物細胞誘發非定標性突變:首先用低濃度( 0 . 2 m )的短壽烷化劑mnng (半壽期為1 . 1hr )處理細胞2 . 5h后,繼續培養24h ,將重組有用作突變檢測的靶基因supftrna基因的穿梭質粒pz189轉入細胞復制,發現在未受致癌物直接攻擊的穿梭質粒中有較自發突變率高5倍以上的靶基因突變。Course contents ( containing practice period ) : introduction to the course ; basic function of cells ; breath ; blood ; blood circulation ; digesting and absorption ; energy metabolism and body temperature ; the excretion of kidney ; sensory organs ; nervous system ; endocrine system ; urogenital system ; physical constitution ; change of physiological function of human body in each sports stage ; lung ' s function measurement ; blood type test ; hemoglobin measurement ; auscultation of heart sounds ; measurement of blood pressure ; electrocardiogram measurement ; measurement of visual field ; measurement of body temperature ; cardiovascular function test before and after sport
課程內容(含實踐活動) :緒論、細胞的基本功能、呼吸、血液、血液循環、消化與吸收、能量代謝和體溫、腎臟的排泄、感覺器官、神經系統、內分泌、生殖、身體素質、運動各階段人體生理功能的變化、肺功能測定、血型鑒定、血紅蛋白測定、心音聽診、血壓測定、心電圖測定、視野測定、體溫測定、運動前後心血管功能變化。Lovrin 10 and cv. 5389. by using this system, following questions were investigated : the change of the pattern of microtubules when treated with iwf ; the change of cytosolic calcium levels in the protoplasts when treated with iwf and the influence of microtubule depolymerization prior to iwf treatment on the levels of cytosolic calcium
試驗的目的是以激發子?原生質體簡化試驗系統來模擬葉銹菌侵染小麥葉片的互作體系,探討激發子刺激后寄主植物微管骨架的動態變化及胞內ca ~ ( 2 + )水平的變化規律,為進一步探討激發子誘導防衛反應的信號轉導途徑奠定基礎。Be probably related with the change of gaba releasing in the brain. in order to testify the effect of nos inhibitor and gaba on the regulation of the sleep / waking cycle in adult rats implanted for chronic sleep recordings, the polysomnogram was recorded. we observed whether the sleep - suppressing action of nos inhibitor was affected after the administration of the agonists of gaba receptors by subcutaneous route
本研究採用多導睡眠描記技術和皮下給藥方式,觀察應用gaba受體激動劑后,對nos抑制劑引起的睡眠抑制效應有無影響,同時用免疫組化的方法,觀察應用nos抑制劑后大鼠額葉皮質2區( frz區) gaba免疫反應( gaba一ir )陽性細胞表達的變化,從而探討no和gaba在睡一醒周期調控中的作用。One is as signal transducers outside of the nucleus, named nongenomic action. the other is as transcription activators in the nucleus, named genomic action. the two ways may coexist, cooperate and coregulate in the development and maturation of oocyte
類固醇激素受體在卵母細胞中可能通過兩種途徑發揮作用,一種是存在於胞質中,作為信號轉導因子的非基因組調控機制;另一種是存在於核中,作為核轉錄活性因於,改變基因轉錄活動的基因組調控機制。The bacteria planococcus sp., pseudomonas sp. and xanthomonas sp. obtained from the dielectric oil of a scrapped large - size transformer were studied on their states and metabolism
摘要從報廢的大型變壓器介質油中分離得到動性球菌、假單胞菌、黃單胞菌等細菌,並對其形態和代謝進行研究。With bacterial cgc as main subject, the tests had been done to elucidate mechanism of self - organization for macroscopic rhythmic structure. the dynamics of cgc forming was observed by special techniques of waving culture and microscopic culture ; the differences in outer structure of cell wall and flagella number had been observed by atomic force microscope scanning ; integrity of cell wall was examined under tem ; outer membrane protein was analysed by sds - page and various substance and factors for cgc formation were determined
採用特殊的波動培養和顯微培養技術觀察潛生體形成動態;應用原子力顯微鏡掃描,比較細菌潛生體與繁殖體在細胞壁外層結構和鞭毛數量的差別;用透射電鏡觀察細胞壁完整性,以十二烷基硫酸鈉?聚丙烯酰胺凝膠電泳分析外膜蛋白的改變,並通過實驗分析多種物質和因素對潛生體形成的影響。Putney jw, jr broad lm, braun fj, et al. mechanisms of capacitative calcium entry j. t cell sci, 2001, 114 : 2223
薛慎伍,張兆巖,李瑋.皮質下動脈硬化腦病患者外周血紅細胞和血小板內鈣離子含量的變化j .中國動脈硬化雜志, 2004 , 12 : 713Non - homogenized dynamic method of cell ( nhdmoc ) is a method of investigating stress wave propagation in laminated materials, the formula and initial boundary conditions were derived for the numeric simulation of stress wave propagating with nhdmoc under the condition of one dimension strain, the corresponding program was designed and checked
非均質化動力學元胞法( nhdmoc )是研究層合材料中應力波的新方法,本文整理、推導了應用nhdmoc數值模擬一維應變條件下應力波的計算公式以及初值條件,編制了計算程序,並進行了驗算。分享友人