胰島培養 的英文怎麼說
中文拼音 [yídǎopéiyǎng]
胰島培養
英文
islet culture- 胰 : 名詞[生理學] (胰腺) pancreas
- 培 : 動詞1. (在根基部分堆上土) bank up with earth; earth up 2. (有目的地使成長、壯大) cultivate; foster; train
- 養 : Ⅰ動詞1 (供養) support; provide for 2 (飼養; 培植) raise; keep; grow 3 (生育) give birth to ...
- 胰島 : [解剖學] langerhans islet; islands of langerhans; pancreatic island; langerhan s island; pancreas...
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Further study on the monolayer culture method of human fetal islet cells
人胎胰島細胞單層培養方法的進一步探討2. morphologic studies of hepatocytes in different nutritive elements medium. objective : this study was designed to determine if different nutritive elements, such as transferrin, insulin, nicotinamide, p - mercaptoethanol ( - me ) and hepatocytes growth factor ( hgf ) affected the morphology of the primary hepatocytes in short term culture
目的:本研究在普通培養液中添加轉鐵蛋白、牛胰島素、煙酷胺、 p琉基乙醇和促肝細胞生長因子,觀察這些營養成分對原代短期培養的小鼠肝細胞的形態學的影響,旨在探討一種適用於原代肝細胞的培養方案。Methods : hepatocytes obtained from mice with in situ portal vein collagenase perfusion were inoculated on two types of medium, which one was 10 % pcs rpmi 1640 medium ( common medium ), the other was rpmi 1640 medium supplemented with 5 u g / ml tranferrin, 5 u g / ml insulin, 10nm nicotinamide, 5mm - me, 40 u g / ml hgf ( special medium )
一種是在rpmi1640培養液中加入了511iml轉鐵蛋白、 5pg ml牛胰島素、 10nm煙酚胺、 smm卜琉基乙醇, 4011g ml促肝細胞生長因子,簡稱為肝細胞培養液。In this experiment, radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process. methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0. 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al. thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum, and added to 50ml cell culture flasks
方法:胰島的分離參照王玲等的方法,每次實驗取新生3 - 5天sd大鼠,無菌條件下剖腹取出胰腺,剪切為0 . 5mm ~ 3的組織塊, v型膠原酶消化30min后,離心洗滌,懸浮於完全培養基,接種入50ml培養瓶,於5 co _ 2 、 95空氣條件下培養20h ,轉板純化,接種於96孔培養板培養24h ,按實驗要求進行實驗。Prolonged the culture term, hepatocytes both of experimental groups and control groups became pyknotic and were detached from the wall or fibroblasts became prominent in the culture pl ate. it was important to note that none of the condition caused an increase in the number of cell in the whole experimental process. conclusion : the primary hepatocytes in medium of additional special nutritive may benefit in comparison with common medium
結論:在培養液中添加轉鐵蛋白、牛胰島素、煙酚胺、 p琉基乙醇以及促肝細胞生長因子,可以促進原代小鼠肝細胞的貼壁生長,並且可以改善肝細胞在體外的代謝,使之維持良好的生活狀態並且延長體外生存時間。Using arious cell and molecular techniques, the researchers discoered that insulin stimulates human keratinocytes in culture to proliferate and migrate
通過各種細胞分子技術,研究者發現胰島素對培養的人角質化細胞有促進增殖和擴散的作用。Using various cell and molecular techniques, the researchers discovered that insulin stimulates human keratinocytes in culture to proliferate and migrate
通過各種細胞分子技術,研究者發現胰島素對培養的人角質化細胞有促進增殖和擴散的作用。The effects of glp - 1 ( 7 - 36 ) nh2 on insulin secretion glp - 1 ( 7 - 36 ) nh2 with the concentration of 2. 5nmol / l, 5. 0nmol / l, l0. 0nmol / l, 20. 0nmol / l, 40. 0nmol / l respectively were added to the medium as different experimental groups, 24 hours later, insulin amount are 68. 76 ? 1. 71 72. 30 ? 3. 13 104. 16 ? 5. 57 110. 98 ?. 29 111. 58 ? 0. 65miu / l respectively, and the insulin account is 55. 53 ?. 63miu / lin the control group. there was no significant difference between the groups with 2. 5nmol / land 5. 0nmol / l glp - 1 ( 7 - 36 ) nh2 respectively ; and there was not significant difference among the groups with lo. onmol / l, 20. 0nmol / l and 40. 0nmol / l glp - 1 ( 7 - 36 ) nh2 respectively. but the difference is significant between experimental groups and control group ( p < 0. 05 ). the data show that with the rising concentration of glp - 1 ( 7 - 36 ) nh2, there is an increasing amount of insulin
對照組培養液中不含g廿一1 ( 7一36 ) nhz ,實驗組培養液中含有20nmol / lglp一1 ( 7一36 ) nhz ,培養24h后,用0 . 25 %胰蛋白酶消化胰島分散細胞,塗片后利用針對胰島素mrna的寡核甘酸探針進行細胞原位雜交, dab顯色,高清晰度病理圖文分析系統( highpathologiealimageanalysissystem , hp認s )對細胞著色的平均光密度( mean即tiealdensity , mod )量化分析,觀察實驗組和對照組胰島素mrna的表達情況。Establishment and comparison of two different methods of cat islet cell culture
貓胰島細胞二種培養方法的建立及比較研究In cultured huma microascular endothelial cells, the insulin stimulates only migration into the wound tissue
在培養的人微血管內皮細胞中,胰島素僅僅對傷口處細胞有促進作用。Experimental condition optimization study on inducing mouse embryonic stem cells differentiation into insulin producing cells by serum - free culture in vitro
無血清培養法誘導小鼠胚胎幹細胞分化為胰島素分泌細胞的實驗條件優化選擇In cultured huma microvascular endothelial cells, the insulin stimulates only migration into the wound tissue
在培養的人微血管內皮細胞中,胰島素僅僅對傷口處細胞有促進作用。分享友人