脫氫奎尼酸酶 的英文怎麼說
中文拼音 [tuōqīngkuínísuān]
脫氫奎尼酸酶
英文
dehydroquinase-
4. engineering dhqase ( arod ) - deficient e. coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e. coli chromosome. the mutant 31bk was engineered, in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e. coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system. the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene, so it improved carbon flow into the quinic acid biosynthesis direction
構建宿主菌基因精確定位突變株31bk （ arod : : arobkan ~ r ）為了改變代謝途徑脫氫奎尼酸（ dhq ）分支點上的代謝流量，使之充分流向目的產物奎尼酸合成方向，利用基因打靶技術構建了31884宿主菌arod基因精確定位插入突變體，使dhq脫水酶（ dhqase ）失活，阻斷了碳代謝流流向芳香氨基酸生成的方向，同時用同源重組的方法將arob基因定位整合入染色體上，解除了限速酶對碳代謝流通過共同途徑到達dhq的阻遏影響，並減輕代謝負擔。2. cloning and functional expression of a heterogenous gene from a. nidulans in e. coli strain a. nidulans chromosome dna was used as template to amplfy gene qutb by pcr, the dna sequencing showed that the cloned gene was in agreement with the reported sequence
異源奎尼酸脫氫酶（ qdhase ）在大腸桿菌中功能性表達以構巢麴黴菌（ a . nidulans ）基因組dna為模板，用pcr方法擴增得到了qutb基因，序列測定與文獻報道一致。
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