膜誘導抗原 的英文怎麼說
中文拼音 [móyòudǎokàngyuán]
膜誘導抗原
英文
membrane-induced antigen- 膜 : 名詞1. [生物學] (像薄皮的組織) membrane 2. (像膜的薄皮) film; thin coating
- 誘 : 動詞1. (誘導) guide; lead; induce 2. (使用手段引人隨從自己的意願) lure; seduce; entice
- 導 : 動詞1. (引導) lead; guide 2. (傳導) transmit; conduct 3. (開導) instruct; teach; give guidance to
- 抗 : Ⅰ動詞1 (抵抗; 抵擋) resist; combat; fight 2 (拒絕; 抗拒) refuse; defy 3 (對等) contend with...
- 原 : Ⅰ形容詞1 (最初的; 原來的) primary; original; former 2 (沒有加工的) unprocessed; raw Ⅱ動詞(原...
- 誘導 : guide; lead; induce; guidance; induction
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So it can induce stronger and earlier iga immune responses at almost all mucosal sites than oral route. the way induced by nasal route is similar to which gut associated lymphoid tissue ( galt ) induced
鼻腔含有的蛋白水解酶較少,抗原不易被破壞,鼻腔免疫可以在多個粘膜部位產生siga反應,誘導與胃腸相關淋巴組織( galt )形式上相似的粘膜免疫反應。Conclusion : a 496 bp fragment of the gene encoding t gondii p22 surface protein was successfully cloned into the plasmid pthiohisa, b, c and expressed in the e. coli top 10, and the fusion proteins can be purificated effectively
結論:成功構建弓形蟲zs株表膜抗原p22編碼是因pthiohisa , b , c p22表達質粒,誘導表達弓形蟲農牧抗原p22蛋白,兒g 。The homologious comparison proved the cloned gene had 96 % homology to the sequence of the omp gene, and the alignment of the amino acid sequence was 98 %. the recombinant plasmid was constructed with the target gene and the expressing vector pgex - 4t - l and then was transformed into e. coli bl21 ( de3 ) the fusion protein was expressed under the iptg inducing condition, and exhibited about 62kda in size, very close to the predicted molecular weight of gst - momp. furthermore, the fusion protein was specifically recognized by anti - serum which raised against the major outer membrane protein of ahl316
Sds - page電泳分析顯示誘導表達的基因產物分子量約為62kda ,與預測的gst -外膜蛋白重組融合蛋白的分子量極為相似, western - blot進一步證實,表達產物能被嗜水氣單胞菌l316主要外膜蛋白特異性抗血清所識別,產生明顯的染色條帶,說明所表達的基因產物與天然的外膜蛋白抗原性一致。The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2, ha order to prepare ndrg2 antibody, the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector. after induced by iptg, the fusion proteins were expressed in e. coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization, rabbits produced high titer antiserum against ndrg2. then antisemm was absorbed using ndrg2 antigen immobilized on nc filters, the purified product of antiserum shows high special to ndrg2 protein, and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach
為制備高效價的ndrgz抗體,分別構建了prset a雌、 pgex4t d唾倉和pgex4tl七三種原核重組表達質粒,並在大腸桿菌中誘導表達出相應的融合蛋白;用全長gstjqdrgz蛋白免疫兔,然後用gst ndrgz人和gstjqdrgze片段加強免疫,經免疫得到了較高效價的兔抗人ndrz多克隆抗血清,利用固定於硝酸纖維素膜上的ndrgz抗原親和吸附純化抗血清,提高了ndrgz抗體的特異性;並對包涵體形式表達的6his ndrgz進行初步的分離純化。分享友人