色氨酸合酶 的英文怎麼說
中文拼音 [shǎiānsuāngě]
色氨酸合酶
英文
tryptophansynthase-
4. engineering dhqase ( arod ) - deficient e. coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e. coli chromosome. the mutant 31bk was engineered, in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e. coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system. the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene, so it improved carbon flow into the quinic acid biosynthesis direction
構建宿主菌基因精確定位突變株31bk （ arod : : arobkan ~ r ）為了改變代謝途徑脫氫奎尼酸（ dhq ）分支點上的代謝流量，使之充分流向目的產物奎尼酸合成方向，利用基因打靶技術構建了31884宿主菌arod基因精確定位插入突變體，使dhq脫水酶（ dhqase ）失活，阻斷了碳代謝流流向芳香氨基酸生成的方向，同時用同源重組的方法將arob基因定位整合入染色體上，解除了限速酶對碳代謝流通過共同途徑到達dhq的阻遏影響，並減輕代謝負擔。Methods : hyperosmotic pressure animal model was established by administering 3 % sodium chloride as drinking water to rats or increasing osmotic pressure of the culture medium. osmoregulation positions in the brain, reciprocal projection pathways between the medullary visceral zone ( mvz ) and supraoptic nucleus ( son ) or hypothalamic paraventricular nucleus ( pvn ), oscillation of intracellular calcium in cultured neurons and astrocytes were studied by means of anti - fos, glial fibrillary acidic protein ( gfap ), tyrosine hydroxylase ( th ) or vasopressin ( vp ) multiple imrnunohistochemical staining, immuno - electronic microscope, wga - hrp retrogradely tracing and cell culture methods. results : ( 1 ) fos positive neurons within the mvz, parabrachial nuclei, locus ceruleus, pvn, son, subfomical organ increased markedly
方法：通過給予大鼠飲用3氯化鈉或提高培養基滲透壓濃度的方法復制高滲刺激模型，主要採用抗fos 、膠質原纖維酸性蛋白（ gfap ）和酪氨酸羥化酶（ th ） （或加壓素? vp ）免疫組織化學多重染色、免疫電鏡、 wga - hrp束路追蹤結合免疫組織化學多重染色、細胞培養等實驗方法，系統觀察了中樞參與滲透壓反射的調控部位、下丘腦視上核（ son ）神經元? ast超微結構的變化、延髓內臟帶（ mvz ）和son及下丘腦室旁核（ pvn ）之間往返投射通路和神經元的性質及其與ast的關系、培養神經元和ast內鈣波的變化。The results showed mn and ni complexes possibly bind to dna by the mode of interaction, whereas zn complex possibly bind to dna by the modes of interaction and electrostatic binding. 5. in addition, we conjugated cleavage system with recognize system and analyzed joint products by hplc, which provide experimental basic for design of dual effects cleavage
此外，本文還選用咖啡酸純品來突破切割體系與識別體系（用氨基臂修飾的寡聚脫氧核苷酸）的連接，並用高效液相色譜法分析其偶聯產物，為今後設計併合成一種具有特異識別和高效切割雙重功能的人工核酸酶提供了實驗基礎。Sleep / waking cycle is a complex network modulation and many factors such as interleukin - 1 ( il - 1 ), tumor necrosis factor ( tnf ), growth hormone releasing hormone ( ghrh ), vasoactive intestina polypeptide ( vip ) and many conventional neurotransmitters such as serotonin ( 5 - ht ), acetylcholine ( ach ), norepinephrine ( ne ), dopamine ( da ) and gamma - aminobutyric acid ( gaba ) were involved in it. recent evidence has shown that no synthesized in neurons in several areas of the brain can induce the release of neurotransmitters. in the rat central nervous system, the anatomical distribution of nos - containing neurons is now well established, and it was reported that nos is co - localized with neurotransmitters well known for their involvement in sleep mechanisms, i. e. 5 - ht, ach, da and gaba
鄭州大學2003屆碩士畢業論文gaba受體激動劑消除no合成酶抑制劑對大鼠睡/醒周期的影響睡/醒周期的形成是一個復雜的網路調控的結果，體內許多因子都參與了這一調控網路，這些因子如白介素一1 （ il一1 ） 、腫瘤壞死因子（ tnf ） 、生長激素釋放激素（ ghrh ） 、血管活性腸膚（ vip ）以及經典的神經遞質如5一輕色氨（ 5一ht ） 、乙酞膽堿（ ach ） 、去甲腎上腺素（ ne ） 、多巴胺（ da ）和卜氨基丁酸（ gaba ）等，它們在睡眠的發生和調節中也發揮著重要作用。Induced either by iptg or l - tryptophan in the absence of easily metabolized carbon such as glucose the strain bl21 ( de3 ) / pet28c - tnaa can express tryptopanase. the fermentation conditions were optimized respectively. in the presence of glucose and iptg as induce agent, the concentration is the crucial factor for expression of active tryptophanase. if the iptg concentration is less than 0. 2mm, the optimum temperature is 37 lower temperature is necessary to obtain active tryptophanase in the case of higher concentration of iptg
用iptg誘導表達實驗結果表明：利用t7啟動子在胞內能表達出有活性的酶； iptg與溫度的合適組合可以得到較高活性的色氨酸酶，在0 . 2mm以下濃度的iptg誘導時， 37是最適溫度，而用較高濃度的iptg誘導，低溫是表達有活性的色氨酸酶的必要條件，而且誘導時間要短。Using pcr technology, a 2. 4kb dna fragment, part of tryptopanase operon, containing a promoter, a regulator gene tnac located downstream of the promoter and a desired tryptopanase gene tnaa which can be expressed by the promoter from e. coli k - 12, was cloned to pmd18 - t vector. the dna sequence is the same as which was published on science
為了證明質粒上的基因能表達出有活性的色氨酸酶，將這個dna片段克隆到pet28c質粒的bamhi和hind位點上，使該片段受t7rna聚合酶的啟動子控制，然後轉化噬菌體de3的溶源菌bl21 （ de3 ） 。Abstract : the diferrences in cell growth, composition and levels of anthocyanins and catechins, activity of phenylalanine ammonia - lyase and dynamics of production between anthocyanin accumulating and non - accumulating subclones of tea cultured cells were examined
文摘:從細胞生長、色素和兒茶素次生產物組成與含量、苯丙氨酸解氨酶活性以及產物合成動態分析幾方面，研究比較了積累色素與不積累色素的兩類茶樹培養細胞系之間的差異。分享友人