菌斑基質 的英文怎麼說
中文拼音 [jūnbānjīzhí]
菌斑基質
英文
matrix of bacterial plague-
In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported
在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。The 1. 488kb dna fragment was sequenced and ligated to pbi121 vector and transformed into otsa deficient of e. coli strains ( ff4169 ). otsa gene is in charge of trehalose - 6 - phosphate synthesis in e. coli. in growth curve experiment, the transformants that carried the 1. 488kb dna fragment grew well in minimum medium, which contains 0. 5mol / l nacl, while control strains could n ' t endure it
提取釀酒酵母的總dna ，以此為模板，採用pcr的方法從釀酒酵母中克隆出了1 . 488kb的海藻糖合成酶基因tps1片段，通過xba和sma雙酶切，與同樣經過xba和sma雙酶切的puc118載體質粒連接，轉入大腸桿菌dh5中，通過藍白斑篩選重組子。In order to construct the recombinant plasmid pcdna3. 1 / ts87, first, the ts87gene fragment was repcred using the redesigned primers to introduce re sites of hindld and bamh i, and kozak sequence. then the rebuilt ts87 fragment was cloned into t - vector and transformed into e. coli dh5 a
通過重新設計引物在ts87兩端加上用於構建重組質粒的酶切位點hind和bamh和用於真核表達的kozak序列。將改造后的基因片段克隆入t - vector ，轉化大腸桿菌dh5 ，通過藍白斑篩選獲得陽性克隆后測序鑒定。
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