藍白斑篩選 的英文怎麼說

中文拼音 [labáibānshāixuǎn]
藍白斑篩選 英文
blue-white selection
  • 藍 : 藍Ⅰ形 （像晴天天空的顏色） blue Ⅱ名詞1. [植物學] （蓼藍） indigo plant2. （姓氏） a surname
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 斑 : Ⅰ名詞（斑點; 斑紋; 污點; 瑕疵） spot; speck; speckle; stripe; stain Ⅱ形容詞（有斑點或斑紋的） spo...
  • 篩 : 名詞[書面語] （植物名） sedge
  • 選 : Ⅰ動詞1. （挑選） select; choose; pick 2. （選舉） elect Ⅱ名詞（挑選出來編在一起的作品） selections; anthology
  • 白斑 : hickie; leukasmus; vitiligo; floccosoids （如鐵杉）白斑病 leucoplakia; leucoderma; leucodermia; m...
  • 篩選 : dressing by screening; screen; preparation by screening; preparation; choose by means of a sift; ...
  1. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  2. After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence

    F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。
  3. The e2 gene was amplified by rt - pcr, then examined the fragment by electrophoresis. after purification and insertion into pucm - t vecter, the recombinant plasmid pbne2pi and pbne2pii were obtained. then they were transfected e. coli jm109 and screened positive clones by blue or white plaques. the recombinant plasmids were extracted and the inserted fragments were identificated by electrophoresis

    通過rt - pcr擴增e _ 2基因，電泳測定擴增片段的大小，經純化后，連接于pucm - t載體，獲得重組質粒pbne2p 、 pbne2p ，轉化e ? colijm109 ，經藍白斑篩選，挑取陽性克隆，提取質粒，直接電泳鑒定和酶切鑒定。
  4. The immunization effects of b95 can be disturbed by hi20 if the immunized ? interval less than 10 days with the normal dose

    將此片段與pbluescriptsk載體連接，轉化大腸桿菌tgi ，經藍白斑篩選，鑒定陽性克隆后測序。
  5. 713bp and 700bp specific fragments were amplified by pcr and ligated into pgem - t easy vector. it was identified by restriction endonuclease digest analysis, pcr and sequencing that this fragment contained the complete open reading frame ( orf ) of the hc and ha gene

    擴增產物連接到pgem - teasy載體上，轉化入大腸桿菌jm109中進行藍白斑篩選后，用酶切、 pcr鑒定和測序的方法鑒定出重組陽性質粒（ pgem - hc和pgem - ha ） 。
  6. Therefore, a - amylase has been used widely in many industrial fields, such as glucose production, beer brewing, fermentation trade, textile industry, and so on. the study on a - amylase is one of the most active fields in enzyme industrial fields. with the development of biotechnology, more and more scientists and researchers attempt to use dna shuffling technology to breed, screen and even " create " new a - amylase genes with higher activity and other new characters

    設計引物時，上下游引物5 』端分別添加了kpn和bamh酶切位點，克隆得到的基因片段和質粒載體都用kpn和bamh進行雙酶切后，進行酶連、轉化、篩選得到陽性重組子，經過藍白斑驗證、單酶切驗證、雙酶切驗證、 pcr驗證等一系列的驗證后進行測序。
  7. The 1. 488kb dna fragment was sequenced and ligated to pbi121 vector and transformed into otsa deficient of e. coli strains ( ff4169 ). otsa gene is in charge of trehalose - 6 - phosphate synthesis in e. coli. in growth curve experiment, the transformants that carried the 1. 488kb dna fragment grew well in minimum medium, which contains 0. 5mol / l nacl, while control strains could n ' t endure it

    提取釀酒酵母的總dna ，以此為模板，採用pcr的方法從釀酒酵母中克隆出了1 . 488kb的海藻糖合成酶基因tps1片段，通過xba和sma雙酶切，與同樣經過xba和sma雙酶切的puc118載體質粒連接，轉入大腸桿菌dh5中，通過藍白斑篩選重組子。
  8. In order to construct the recombinant plasmid pcdna3. 1 / ts87, first, the ts87gene fragment was repcred using the redesigned primers to introduce re sites of hindld and bamh i, and kozak sequence. then the rebuilt ts87 fragment was cloned into t - vector and transformed into e. coli dh5 a

    通過重新設計引物在ts87兩端加上用於構建重組質粒的酶切位點hind和bamh和用於真核表達的kozak序列。將改造后的基因片段克隆入t - vector ，轉化大腸桿菌dh5 ，通過藍白斑篩選獲得陽性克隆后測序鑒定。
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