蛋清液 的英文怎麼說

中文拼音 [dànqīngyè]
蛋清液 英文
albumin solution
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 清 : Ⅰ形容詞1 （純凈） unmixed; clear 2 （寂靜） quiet 3 （清楚） distinct; clarified 4 （一點不留） w...
  • 液 : 名詞（液體） liquid; fluid; juice
  1. Study on treatment of soybean albumen whey wastewater using pre - oxidation - coagulating sedimentation method

    預氧化混凝處理大豆蛋白乳清廢液技術探討
  2. The antibody of ceruloplasmin is modified on the qcm by the approach above and the determination of ceruloplasmin is carried out in the buffer solution with 3. 5 % polyethylene glycol ( peg )

    採用上述固定化方法，將銅藍蛋白抗體吸附固定於石英晶體表面，在含有3 5的聚乙二醇（ peg ）緩沖溶液中測定人血清銅藍蛋白（抗原） 。
  3. The supernatants were dialyzed against 50 mm phosphate buffer ( pb ) ( ph 6. 5 ), and passed through a blue - sepharose 6b column ( 3x30 cm )

    Iptg誘導表達目的蛋白，上清液經sds一page電泳后看到約32kd的目的條帶。
  4. Transudate : extravascular fluid collection that is basically an ultrafiltrate of plasma with little protein and few or no cells. fluid appears grossly clear

    漏出：屬于血漿的超濾液，含有少量蛋白，極少量細胞，液體澄清。
  5. Human serum albumin ( hsa ) is the most abundant protein in huaman blood plasma, accouting for 60 % of the total serum proteins. it also exists in tissue, body liquid, skin and lymph chamber, and forms the extravascular pool

    人血清白蛋白（ humanserumalbumin ， hsa ）是人血漿中最豐富的蛋白質，約占血漿總蛋白的60 ，還存在於組織、體液、皮膚和淋巴腔中，構成血管外池。
  6. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  7. In this paper, the application of proteomic techniques in cell lines, tissues, serum and pancreatic juice from patients with pancreatic cancer is reviewed briefly

    本文就蛋白質組學技術在胰腺癌細胞系、組織、血清和胰液等方面的應用作一綜述。
  8. This is the land of the elec - tric salad dryer, the electric can opener, the electric soap dispenser, the electric air freshener, the electric hair curler, the in - the - shell egg scrambler, and the electric tweezers

    這是電沙拉乾燥器、電罐頭起子、電皂液器、電空氣清新器、電卷發器、炒蛋器、電拔毛器的國土。
  9. Lysozyme chloride powder has a wide area of application, such as food preserving ( cheese, low degree wine ), pharmaceutical industry ( tablets, capsules, eyedrops, throat lozenges ), daily chemicals ( tooth paste, cosmetics ), baby food ( milk powder ) and scientific research

    蛋清溶菌酶在食品保鮮（特別是乳酪，清酒生產） ，醫藥（藥片，膠囊，眼藥水，潤喉液） ，日化（牙膏，化妝品） ，嬰兒食品（母乳化奶粉）及科學研究中有著廣泛的應用。
  10. The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku

    將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體，然後導入畢赤酵母（ p8chianastoris ）菌株gslls細胞中，在甲醇的誘導下，經過酵母高密度發酵進行pap的表達，經sds page分析，結果表明，在培養基上清液中含有一明顯的特異性蛋臼條帶，大小為34ku ，經western blotting分析，該蛋白與法國pap抗血清有特異性反應，體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性，說明該pap基因在畢赤酵母gs中也得到了正確表達。
  11. The proteins and oligosaccharides contents of soy whey, which originated from the preparation of soy protein isolates prepared with aqueous alcohol washed soy meals, were much lower than that by traditional production technology

    摘要與傳統的大豆分離蛋白生產工藝相比，豆粕經乙醇溶液處理后在大豆分離蛋白制備過程中產生的乳清，其蛋白質和低聚糖的含量明顯降低。
  12. In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company

    實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。
  13. The results of lauryl sodium sulfate - polyacrylamide gel electrophoreses ( sds - page ) of the aggregate precipitate and supernatant and the result of high - performance size - exclusion chromatography of the supernatant indicated that, by wrongly linked intermolecular disulfide bonds soluble bi - molecular and tri - molecular egg white lysozyme aggregate could be simultaneously formed except being renatured to native and active egg white lysozymes during the refolding procedure of denatured - reduced egg white lysozyme ; the aggregate precipitate could be further formed by the non - covalent bonds interaction between the soluble hi - molecular egg white lysozyme aggregates, and the soluble tri - molecular egg white lysozyme aggregate could still stay at the supernatant

    沉澱和上清液的不連續十二烷基硫酸鈉聚丙烯酰胺凝膠電泳（ sds - page ）和高效凝膠排阻層析分析結果表明，還原脲變性蛋白溶菌酶在稀釋復性過程中除了能夠復性成天然態蛋白溶菌酶分子外，還會形成可溶的蛋白溶菌酶分子二聚體和三聚體，二聚體和三聚體主要是靠分子間二硫鍵的錯配連接而成的；可溶的蛋白溶菌酶分子二聚體之間通過非共價鍵相互作用而形成集聚體沉澱，而可溶的三聚體溶菌酶分子則仍處于復性液上清液中。
  14. Biological activity of this fusion protein was detected by vsv / wish system, the result was 4. 104. 1 104iu / m =

    將篩選出來的重組菌株誘導144小時，取菌液上清液用vsv wish系統檢測重組蛋白的抗病毒活性為4
  15. After induced by 0. 1 m iptg, the pellet was resuspended in phosphate - buffered saline ( pbs ) / 5 mm edta, and sonicated three times for 20 s at maximal output

    上清液過blue一sepharose6b柱，進行一步親和層析，得到純化的rta和pj人~ kdel重組蛋白。
  16. Absorbency and amino - nitrogen in soy sauce were determined after treatment with gelatin and egg white

    結果表明，蛋清液是較好的澄清劑，其澄清度高且用量適中；明膠的處理效果稍差，且用量較少。
  17. After ultrafiltration and rough separation with ammonium sulfate and anion - exchange chromatography, the recombination protein of ctla4 extracelluar domain was rectified from the ferment supernatants

    表達菌甲醇誘導發酵；發酵上清液經超濾、硫酸胺粗分級分離以及陰離于交換層析，純化出ctla4胞外區蛋白。
  18. The results of sds - page and western blot showed that the culture supernatant of transforments contained the correct glycosylated e2 protein and the recombinants could still secretedly express the specific proteins after culturing for 8 generations

    經甲醇誘導表達后， sds - page和westernblot結果證明了p . pastoris培養上清液中含有正確糖基化的e2蛋白，表達量約250mg / l 。
  19. After washing with reagent ( 50mmol / l ph8. 0 tris - hcl, 100mmol / l nacl, 0. 5mmol / l edta, 2mol / l carbamide, 0. 2 % triton x - 100, 0. 2 % doc ), ultrasound crushing ( 200 w, 150 times, 3 seconds per time, spacing 3 seconds ) and freezing - melting methods, we got hng fusion protein and m - insulin fusion protein with purity of above 80 %. 4. radioimmunoassay result shows that the radioimmune activity of of m - insulin fusion protein is 0. 5 unit per litre bacterial liquid

    3 .表達產物初步純化仁gly 」一flumanin融合蛋白和人胰島素突變體融合蛋白包涵體的洗滌方案為: lj ]體沉澱溶於含zmol ， / l尿索洗滌液，超聲（ 200w 、巧o次、 3秒/次、間隔3秒） ， 4 、 i000orpm離心15min后沉澱用洗滌液充分重懸，一2 （ ）凍存過夜，次日融化后4 、 10000rpm離心15min ，取上清，即得到初步純化的融合蛋白，可去除土要的雜蛋白，融合蛋白溶解于上清液中，純度達到80 %以_ 1 : 。
  20. The large - scale expression product is concentrated and desalted by ultra - filter, and the target protein is separated by affinity chromatography with chitin, and then cut by cnbr. the purified product appears evident antibacterial activity with method of agar diffusion

    採用優化的表達條件進行大量發酵，對上清液超濾濃縮脫鹽后，採用幾丁質基質進行親和層析分離目的蛋白，溴化氰切割，瓊脂孔穴法測抗菌活性。
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