蛋白印跡 的英文怎麼說

中文拼音 [dànbáiyìnjī]
蛋白印跡 英文
protein blot
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 印 : Ⅰ名詞1 （圖章） seal; stamp; chop 2 （痕跡） print; mark 3 （姓氏） a surname Ⅱ動詞1 （留下痕跡）...
  • 跡 : 名詞1 （留下的印子; 痕跡） mark; trace 2 （前人遺留的建築或器物等） remains; ruins; vestige 3 （...
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. Recovery of this photoinhibition is a complicate but orderly course, including degradation of photodamaged d1, synthesis and assembly of new one, etc. using lincomycin to block the replacement of new synthetic dl protein into photodamaged one, the spinach leaves was exposed to highlight, giving rise to photoinhibition before the thylakiod membranes were isolated

    解除光抑制后， ps活性恢復是一個復雜而有序的過程，需要d1蛋白降解、新合成d1蛋白和重組裝ps等。實驗首先進行菠菜葉片光抑制處理，加入林可黴素阻斷葉綠體蛋白質合成，利用尿素sds變性電泳分離類囊體膜蛋白，藉助d1蛋白抗體westen免疫印跡、磷酸化蛋白快速檢測方法分析d1蛋白存在形式，並進行定量分析。
  2. Ip3 - ip3 receptor ( ip3r ) interaction mediates the release of ca2 + from the endoplasmic reticulum in response to many different extracellular stimulus. for higher plants, however, though it is now generally accepted that ip3 participates in signal transduction in many important cellular processes, only limited evidence is available for the presence and properties of the ip3r - like protein so far. here, using the immunological methods with an antibody raised against a mammalian inositol 1, 4, 5 - triphophate receptor ( type 1 ), we found that, 1 ) the antibody across - reacted the proteins with about 200kd in microsomes from oryza sativa and about 200kd from arabidopsis thaliana respectively

    本實驗用sds - page電泳和免疫印跡的方法，用哺乳動物大鼠三磷酸肌醇受體的多肽做抗體對類三磷酸肌醇受體蛋白鑒定，結果表明：抗體與水稻和擬南芥微粒體蛋白分子量大約為200kd的蛋白交叉反應，同時還發現在水稻微粒體蛋白62kd和擬南芥微粒體蛋白45kd處有交叉反應的蛋白條帶存在，表明在植物中有類三磷酸肌醇受體蛋白的存在；用免疫膠體金方法，發現類三磷酸肌醇受體蛋白主要分佈於液泡膜和細胞質膜上。
  3. The protein - storing cells in swietenia macrophylla were found to be populus - type, i. e. ordinary parenchyma cells containing both vacuolar protein inclusions and starch grains

    用21kda蛋白質的抗血清進行兔疫印跡分析表明， 18kda蛋白質和21kda蛋白質有相同的抗原決定簇。
  4. The positive colonies that grew on the ampicillin ( amp ) plate ( lb agar medium contaning 100 g / ml amp ) were screened and identified. sds - page and western blot analysis were performed to study the expression profiles of target gene cein in e. coli

    從轉化的平板中篩選出陽性重組子，進行不同iptg濃度和不同誘導時間的表達研究，並利用sds - page電泳和westernblotting蛋白質印跡技術對外源基因cei _ （ 12 ）在大腸桿菌e
  5. Anti - if - protein antibodies were used as probes for immuno - fluorescence, and the reactions of them with different parts of the cell were detected, which suggested the possibility of the existence of the intermediate - like filaments in the cytoplasm. those proteins homologous to the antibodies distributed regularly in the protoplasm. to characterize the corresponding proteins, sds - page and immunoblots were utilized. the 21, 23, 33 and 68kd proteins were distinguished among the diverse protein constituents of the cell. some of these proteins also showed the cross - reactivities with anti - if - proteins antibodies derived from higher organisms. these two evidences both contributed to the homology of some proteins in

    以抗中間纖維蛋白抗體作為探針進行免疫熒光實驗，得到細胞內不同部位的陽性反應，暗示原生質中可能存在類中間纖維。這些同源蛋白的胞內分佈具有一定的規律性。進一步採用sds - page和免疫印跡技術研究它們的生化性質，發現4種主要蛋白明顯有別于胞內其他蛋白組分。
  6. By sds - page and immuno - blotting, the monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain could recognize the 67 kda protein in purified golgi apparatus fraction from lily pollen. subsequently by immuno - gold labeling and transmission electron microscopy, we found that the dynein intermediate chain - like protein bound mainly to the membranes of golgi - associated vesicles. statistics analysis of dynein intermediate chain - like protein on golgi - associated vesciles showed the nearly equal chance of distribution on either cis - or trans - golgi - associated vesciles

    對分離純化的百合花粉及花粉管中高爾基體組分進行sds -聚丙烯酰胺凝膠電泳和免疫印跡發現，抗雞腦細胞質力蛋白中間鏈單克隆抗體在67kda處有較強的免疫交叉反應；進而通過免疫金標結合電子顯微鏡觀察發現，大多數類細胞質力蛋白中間鏈存在於高爾基體附近的囊泡膜上；統計結果表明，類細胞質力蛋白中間鏈在順面和反面高爾基體附近囊泡膜上的分佈機率大致相等。
  7. Analysises of serum amyloid protein in senile dementia patients using immuno - slot blot

    老年期癡呆患者血清澱粉樣蛋白免疫印跡分析
  8. To investigate the functions of these factors, cells were treated with anti - c - fos, anti - c - jun and anti - ras antibodies and examined microscopically for the cell cycle progression. the results indicated that c - fos - like protein, c - jun - like protein and ras - like protein played important roles in checkpoint regulation in physarum polycephalum. western blot analysis showed that the c - fos - like protein and c - jun - like protein may act in cell cycle checkpoint by changing the cyclin b1 - like protein and p53 - like protein expression ; and the ras - like protein may act by changing the cyclin bl - like protein, p53 - like protein, c - fos - like protein and c - jun - like protein expression

    蛋白免疫印跡分析的結果表iv明，當s期、 gz期和前期細胞內具有功能活性的類ras蛋白的量減少時，細胞中的細胞周期調控因於類cyclinbi蛋白、類c jun蛋白、類c fos蛋白和類p53蛋白的表達水平，與未經抗體處理時相比發生了變化（或升高或下降） ；而山抗體處理引起的多頭絨泡菌細胞內具有功能活性的類c fos和類c jun蛋白的減少，使細胞內類cyclinbi蛋白和類p53蛋白的表達水平與抗體未處理時相比也發生了明顯的改變。
  9. Based on the data arising from the experiments in this thesis, a hypothesized model, which intends to explain the mechanisms and relationship between histone acetylation and the expression of important cell cycle regulating factors in physarum polycephalum. the main results and conclusions of this thesis are as follows. 1

    通過1 」 sa處理、 rt干cr和蛋白兔疫印跡實驗，發現多頭絨泡菌細胞中存在著哺乳動物細胞周期調控相關回于的同源物，包括細胞周期素類cyclinbi蛋白、癌基因產物類c foo和類c j 。
  10. Methods : 1 ) 12h after irradiation, the cell cycle of nih3t3 cells was determined by flow of cytometry and the ratio of alternations in p16 gene exon - 2 was evaluated through pcr - sscp. 2 ) the content of mda, the activities of the sod and gsh - px in the supernatant of nih3t3 cells and the cells were measured by detecting kits immediately after irradiation. 3 ) the level of matrix metalloproteinase - 2 ( mmp - 2 ) in hela cells was detected by western - blotting and dot - blotting 2h after irradiation

    具體方法為： （ 1 ）照射后12h ，收集nih3t3細胞，用流式細胞儀檢測各組細胞的細胞周期， pcr - sscp檢測抑癌基因p16的變化； （ 2 ） nih3t3細胞照射后立即收集細胞和細胞上清，用試劑盒測量mda含量和sod 、 gsh - px的活性並觀察其變化； （ 3 ） western免疫印跡和點雜交法檢測照射2h后的各組hela細胞中基質金屬蛋白酶- 2 （ mmp - 2 ）的表達變化。
  11. Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid, and induced with methanol to secrete interesting peptide. the supernate of pichia pastoris culture was analysed by sds - page and western blotting. a reactive band, which the apparent molecular weight is 36kd, can be detected with sheep anti - hcmv polyclonal antibodies

    重組質粒轉化巴氏畢赤酵母， g418篩選出多拷貝插入的單克隆，甲醇誘導多拷貝插入的單克隆酵母細胞分泌目的蛋白，培養液上清經sds - page電泳分析，在蛋白質印跡中檢測到培養液上清有一表觀分子量為36kd ，能與羊抗hcmv多克隆抗體發生發應的條帶。
  12. The results of sds - page and western blot showed that the product was recombinant poifn - a fusion protein

    印跡實驗，結果表明表達產物為重組豬口乾擾素融合蛋白。
  13. This understand of stored nitrogen compounds restricted seriously the progress in the investigation of vegetative storage proteins. in the dissertation, we studied more extensively the cytology, biochemical properties and biological roles of vegetative storage proteins in swietenia macrophylla and in hevea brasiliensis by light - and electron microscopy, sds - page, page, immuno - blotting, indirect immunohistochemical localization and colloidal gold labelling and cdna clone techniques

    採用光鏡和電鏡技術、 page 、 sds - page和免疫印跡技術、電泳凝膠過碘酸? schiff試劑染色、間接免疫熒光和電鏡免疫細胞化學定位技術以及cdna克隆技術，較深入地研究了大葉桃花心木和巴西橡膠樹的營養貯藏蛋白質的細胞學、生物化學性質和生物學功能。
  14. In this paper, the effect of nuclear actin on the process of chromosome construction has been studied by utilizing the precise natural synchrous plasmodium of physarum polycephalum, sds - polyacryl amide gel electrophoresis ( sds - page ), western blotting, the cell - free system and optics microscopy. the major results and conclusions are as follows : 1

    本實驗以多頭絨泡菌原質團為材料，採用同步化培養、細胞核提取、 sds - page 、免疫印跡、非細胞體系構建、光學顯微鏡觀察等方法，研究了有絲分裂前期核內肌動蛋白對染色體構建的影響。
  15. With ni2 + - lda - sepharose, the cbd tag was removed. according to the results of sds - page and western blot analysis, the product of outflow was the target protein lt a 27. the purity of lt a 27 was about 95 %

    運用niz +金屬鰲合親和層析柱進一步快速純化去除融合頭，上樣流出經sds一隊ge分析和western印跡分析ii1 :明得到的純化產物為淋巴毒素缺失體蛋白lt凸27 ， lt 2 :產物純度可達95 %以上。
  16. This review introduces the latest studies of surface functionalization of the poly ( vinylidene fluoride ) ( pvdf ) porous membranes, including synthesizing imprinting membranes using photo - grafting technique and environment - sensitive separation membranes, and immobilizing some proteins and enzymes on membranes

    摘要介紹了聚偏氟乙烯（ pvdf ）微孔膜的表面功能化的研究進展，包括合成分子印跡膜、環境敏感性分離膜和分離膜上蛋白和酶的固定化。
  17. Indentificatiort is also the first step towards studies on protein co - and post - translational modification, and ultimately, function. in the present study, the total proteins of the photo - thermo sensitive genie male - sterile rice ( oryza sativa, peiai64s ) spikelet at meiosis stage were used as the material. by optimizing crucial factors and procedures such as sample treatment, electrophoresis parameters, and gel concentration, 2 - d maps with high quality and reproducibility were obtained

    用兩種方法對經雙向電泳分離的凝膠上的蛋白質點進行了初步鑒定，一是通過電印跡轉移把蛋白質轉到pvdf膜上，再用edman降解的方法測得部分相對分子質量在10000 - 30000da的蛋白質點的n -端序列，通過網上搜索其同源性對其進行鑒定，並確定該點在凝膠上的位置。
  18. 3 ) western blotting of gas1p when mutant gpi17p overexpressed showed that there was accumulation of immature gas1p. this means that there is a defect in gpi - anchoing

    3 ）蛋白質印跡分析表明，在突變gpi17p存在下，有未成熟gas1p的積累，表明存在蛋白質階i化缺陷。
  19. 8kb cdna with 1362bp orf and codes 454 amino acids with only a sh2 domain. the gene was named as sh2a at chromosome 8p22. we study its structure by blast homologous analysis and its expression by rt - pcr and northern blot

    本文將通過蛋白質分析軟體研究其結構和同源性，利用rt - pcr和northern印跡雜交技術研究該基因的表達情況及該基因與腫瘤的關系。
  20. Amino acid molecules imprinted polymeric composite microspheres with magnetic susceptibility ( ms - smips ) were prepared by suspension polymerization ( sp ) and inverse emulsion - suspension polymerization ( iesp ), respectively. protein molecules imprinted soft - wet gel composite microspheres with magnetic susceptibility ( ms - pigms ) were prepared by inverse suspension polymerization ( isp ) and seeded inverse suspension polymerization ( sisp ), respectively. the resulting ms - smips and ms - pigms were characterized by scanning electron microscope ( sem ), thermogravimetric analyzer ( tg ), vibrating sample magnetometer ( vsm ), infrared spectrum ( ftir ), high performance liquid chromatography ( hplc ), and so on

    分別採用懸浮聚合法和反相乳液?懸浮聚合法制備了氨基酸分子印跡聚合物磁性復合微球（ ms - smips ） ；分別採用反相懸浮聚合法和種子反相懸浮聚合法制備了蛋白質分子印跡磁性軟濕凝膠復合微球（ ms - pigms ） ；用掃描電子顯微鏡、熱重分析儀、振動樣品磁強計、紅外光譜、高效液相色譜等對所制備的ms - smips和ms - pigms進行了表徵。
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