蛋白感光液 的英文怎麼說
中文拼音 [dànbáigǎnguāngyè]
蛋白感光液
英文
albumen coating solution- 蛋 : 名詞1. (鳥類或龜、蛇類所產的卵) egg 2. (像蛋形的東西) an egg-shaped thing 3. (辱罵之詞)
- 白 : Ⅰ形容詞1 (似雪的顏色) white 2 (清楚; 明白; 弄明白) clear 3 (空的; 沒加他物的) pure; clear; ...
- 感 : Ⅰ動詞1 (覺得) feel; sense 2 (懷有謝意) be grateful; be obliged; appreciate 3 (感動) move; t...
- 光 : Ⅰ名詞1 (照耀在物體上、使人能看見物體的一種物質) light; ray 2 (景物) scenery 3 (光彩; 榮譽) ...
- 液 : 名詞(液體) liquid; fluid; juice
- 蛋白 : 1. (卵中透明的膠狀物質) egg white; albumen; gary2. [生物化學] (蛋白質) protein
- 感光 : [攝影學] sensitization; photoreception; photosensitive; sensitive; sensitometric感光玻璃 photosen...
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After the incubation in three solutions, the membrane - bound gq a in 42kda was all extracted from photoreceptor cell. the percent of the membrane - bound gq a in photoreceptor cell in high calcium solution, physiological solution and low calcium solution was 4. 63 %, 4. 58 %, and 5. 05 % respectively on light adaptation and was 4. 56 %, 4. 94 % and 5. 13 % respectively on dark adaptation
高鈣溶液、生理溶液、低鈣溶液孵育后的感光細胞中膜結合gq蛋白亞基的含量,光適應組分別是4 . 63 、 4 . 58 、 5 . 05 ;暗適ca2 +濃度對光暗適應時羅氏沼蝦感光細胞中gq蛋白。二 、 effects of calcium concentration on the membrane - bound gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation. the membrane - bound gq a was extracted from the retina of the macrobrachium rosenbergi with protein extract and was electophoresised by sds - page. the percent of the membrane - bound gq a was analyzed by tanon gis gel image disposal system
二、鈣離子濃度對光暗適應羅氏沼蝦感光細胞膜結合gq蛋白亞基的影響用蛋白質提取液提取膜結合gq蛋白亞基並sds - page電泳,利用tanongis凝膠圖像處理系統分析其含量。In our experiment, after light and dark adaptation, the retina of the macrobrachium rosenbergi was respective incubated in high calcium solution, physiological solution and low calcium solution. we studied the effect of calcium concentration on the content and subcellular localization of gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation by sds - page technology and imunoelectron microscopy technology. our study results indicated : 一 、 effects of calcium concentration on the soluble gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation
而鈣離子對gq蛋白亞基活性有無影響還未見報道。我們以光適應和暗適應條件下的羅氏沼蝦復眼視網膜為材料,分別用高鈣溶液、生理溶液、低鈣溶液孵育后,通過sds ? page電泳技術及免疫膠體金電鏡技術,研究鈣離子濃度對光暗適應時羅氏沼蝦感光細胞gq蛋白亞基含量的影響及亞基亞細胞定位的影響。In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection
此外,為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況,我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中,與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒,將其感染sf9細胞制備p1種子液,同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定,確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。The soluble gq was extracted from the retina of the macrobrachium rosenbergi with protein extract and was electophoresised by sds - page
光暗條件下,在3種溶液孵育后的感光細胞中都提取到了42kda的可溶性gq蛋白亞基。分享友人