蛋白抗體方法 的英文怎麼說

中文拼音 [dànbáikàngtǐfāngfǎ]
蛋白抗體方法 英文
protein antibody method
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 抗 : Ⅰ動詞1 （抵抗; 抵擋） resist; combat; fight 2 （拒絕; 抗拒） refuse; defy 3 （對等） contend with...
  • 體 : 體構詞成分。
  • 方 : Ⅰ名詞1 （方形; 方體） square 2 [數學] （乘方） involution; power 3 （方向） direction 4 （方面） ...
  • 法 : Ⅰ名詞1 （由國家制定或認可的行為規則的總稱） law 2 （方法; 方式） way; method; mode; means 3 （標...
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  • 方法 : method; means; way; technique; process; procedure; plan; device; recipe; fashion; tool; maneuver
  1. A novel protein immobilization approach for piezoelectric immunosensors based on the sam of derivative aa has been proposed, which is formed by coupling merca ptoprorionic acid ( mpa ) to alginic acid sodium salt via edc and nhs. ( 1 ) the tr antibody is immobilized via edc and nhs. comparing with the mpa sam method, this new approach shows greater frequency response and high sensitivity

    應用edc和nhs使褐藻酸鈉與胱胺偶合形成衍生褐藻酸鈉作新的自組裝材料，用於蛋白固定，應用這一新方法， （ 1 ）實現了轉鐵蛋白抗體分子的固定，並對轉鐵蛋白參考血清進行了測定，該方法可在0 . 08 25 . 7 g / ml范圍內對轉鐵蛋白進行測定。
  2. In the experiment, the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked. it was expressed in e. coli and its protein was determined. after having been properly modified, the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing, which is the prerequisite work for genetic transformation

    本實驗從抗除草劑轉基因bobwhite小麥中，利用pcr克隆的方法擴增出bar基因全長，並在原核表達系統中表達，鑒定表達蛋白的活性，將能夠正確編碼ppt乙酰轉移酶的bar基因片段，經過適當的修飾構建入真核表達載體。
  3. The antibody of ceruloplasmin is modified on the qcm by the approach above and the determination of ceruloplasmin is carried out in the buffer solution with 3. 5 % polyethylene glycol ( peg )

    採用上述固定化方法，將銅藍蛋白抗體吸附固定於石英晶體表面，在含有3 5的聚乙二醇（ peg ）緩沖溶液中測定人血清銅藍蛋白（抗原） 。
  4. After the protein refolding of denaturant inclusion body following dialysis, we got the pure recombinant gst - eo protein by gst affinity columns. using the purified protein as coating antigen, an indirect elisa were developed for detecting the anti - eo antibody in the csfv serum by exploring the concentration of coating an tigen and dilution degree of serum

    使用分步透析法對變性的包涵體進行復性，將復性蛋白過gst親和層析柱得到純化的gst - eo融合蛋白。以gst - eo融合蛋白為診斷抗原，初步建立了用間接elisa檢測豬瘟血清eo抗體的方法。
  5. However, it is necessary to acquire the antibody or the antiserum, which could specially react with the expression protein of die objective gene transferred into the transgenic plant according to the characteristics of high homology and immune cross - reaction among plant ferritin, using the special immune serum of pea ferritin, the content of plant ferritin could be detected for studing the ferritin expression of transgenic plant by the technique of immunoassay such as immunoprecipitation, eljsa and western blotting

    利用免疫檢測技術進行植物轉基因的表達檢測是一種簡單、靈敏、快速、可靠的方法，但其前提條件是要有與轉基因植物目的基因表達的蛋白質發生特異性免疫反應的抗體或抗血清。根據植物鐵蛋白之間有高度同源性和交叉免疫反應的特性，利用特異性的豌豆鐵蛋白抗血清，就可通過免疫沉澱、 elisa或western雜交等免疫檢測方法進行植物鐵蛋白含量等的檢測，從而更好地進行轉基因方面的研究。
  6. Porcine transmissible gastroenteristis is an importan contagious disease endangering the development of swine. in other to establish a rapid diagnosis method and provide effective immunogenic products, the nucleoprotein ( n ) gene of porcine transmissible gastroenteristis virus ( tgev ) was cloned. expressed and its expressed product was purified

    為建立對豬傳染性胃腸炎快速有效的診斷方法，並試圖在預防上提供有效的免疫制劑，本論文首次在我國對豬傳染性胃腸炎病毒核衣殼蛋白基因進行了克隆、鑒定、表達及重組核蛋白的純化；並在細胞上對重組核衣殼蛋白抗體的中和效力進行了測定。
  7. Recovery of this photoinhibition is a complicate but orderly course, including degradation of photodamaged d1, synthesis and assembly of new one, etc. using lincomycin to block the replacement of new synthetic dl protein into photodamaged one, the spinach leaves was exposed to highlight, giving rise to photoinhibition before the thylakiod membranes were isolated

    解除光抑制后， ps活性恢復是一個復雜而有序的過程，需要d1蛋白降解、新合成d1蛋白和重組裝ps等。實驗首先進行菠菜葉片光抑制處理，加入林可黴素阻斷葉綠體蛋白質合成，利用尿素sds變性電泳分離類囊體膜蛋白，藉助d1蛋白抗體westen免疫印跡、磷酸化蛋白快速檢測方法分析d1蛋白存在形式，並進行定量分析。
  8. Ip3 - ip3 receptor ( ip3r ) interaction mediates the release of ca2 + from the endoplasmic reticulum in response to many different extracellular stimulus. for higher plants, however, though it is now generally accepted that ip3 participates in signal transduction in many important cellular processes, only limited evidence is available for the presence and properties of the ip3r - like protein so far. here, using the immunological methods with an antibody raised against a mammalian inositol 1, 4, 5 - triphophate receptor ( type 1 ), we found that, 1 ) the antibody across - reacted the proteins with about 200kd in microsomes from oryza sativa and about 200kd from arabidopsis thaliana respectively

    本實驗用sds - page電泳和免疫印跡的方法，用哺乳動物大鼠三磷酸肌醇受體的多肽做抗體對類三磷酸肌醇受體蛋白鑒定，結果表明：抗體與水稻和擬南芥微粒體蛋白分子量大約為200kd的蛋白交叉反應，同時還發現在水稻微粒體蛋白62kd和擬南芥微粒體蛋白45kd處有交叉反應的蛋白條帶存在，表明在植物中有類三磷酸肌醇受體蛋白的存在；用免疫膠體金方法，發現類三磷酸肌醇受體蛋白主要分佈於液泡膜和細胞質膜上。
  9. Among antihypertensive measures, blockade of the raas by means of ace inhibitors or angiotensin ii receptor antagonists recently emerged as a potentially polyvalent approach, not only for treating hypertension and reducing cardiovascular events, but also to prevent or reduce albuminuria, counteract diabetic nephropathy and lower the occurrence of new type 2 diabetes in individuals at risk

    在抗高血壓藥物治療中，通過用ace抑制劑或血管緊張素受體拮抗劑可阻滯腎素-血管緊張素-醛固酮系統激活，最近其可作為一種潛在多能的治療方法，不僅可治療高血壓和減少心血管事件，也能夠預防或減少蛋白尿，阻止糖尿病性腎病變，並使新糖尿病患者發生風險性降得更低。
  10. Purified fusion protein gst - hnadc3 was used as an immunogen to inoculate rabbits and the antibody against the gst - hnadc3 fusion protein was raised, and was purified by gst sepharose 4b affinity chromatography to remove the antibody against gst

    Ptg誘導表達，超聲破碎細胞后，採用親和層析方法純化融合蛋白gst十nadc3 ，並以此為抗原免疫紐西蘭株白兔制備融合蛋白抗體。應用親和層析的方法對gst十nadc3融合蛋白抗體進行純化，以去除抗gst抗體。
  11. This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected

    本研究以蛋白質分子設計的理論和方法研究避孕疫苗，將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合，獲得新抗原- 35肽序列；並在合成、純化後分別與弗氏佐劑、免疫刺激復合物（ iscoms ）混合后免疫不同遺傳背景的雌性小鼠，觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變：以及在ivf下，新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。
  12. In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company

    實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。
  13. With the base of related research on nucleic acid immunization, the technology was used to develop th e research and application of cpti transgenic plant. a new antibody preparation method by nucleic acid immunization to assay the expression of the transgenic gene was explored and compared with the traditional one which immune animal by protein

    在國內外大量核酸免疫的研究基礎上，本研究首次將核酸免疫技術應用於轉基因植物檢測研究中，探討一種核酸免疫法制備抗cpti抗體來檢測基因表達的方法，並與傳統的蛋白免疫方式制備抗體進行比較。
  14. So piezoelectric immunnosensors is gradually applied to some fields such as biochemical research, clinical analyze, environment and industry process assay. according to different method of immobilized antigens or antibodies, developed some new piezoelectric immunnosensors to determine proteins in consult serum

    湖南大學分析化學碩士論文李丹200年6月本文結合壓電免疫傳感器抗體（抗原）固定化方法的不同，研製了一些新的壓電免疫傳感器用於參考血清中的一些蛋白的測定。
  15. In order to reveal the mechanisms of extreme radioresistance and dna repair in deinococcus radiodurans, we examined proteome changes in a wild type strain following y - irradiation using 2 - dimensional polyacrylamide gel electrophoresis and silver staining

    為了研究耐輻射球菌極強的輻射抗性與dna修復機理，我們應用雙向電泳結合銀染的方法考察了野生型菌株kd8301在射線照射前後細胞體內總蛋白組的變化情況。
  16. The purpose of this study was to elucidate the biological function of hnadcs and its relationship to renal disorders. methods : the biochemophysical characteristics and secondary structure of amino acid sequence of hnadcs were analysed via software on - line ( http / / expasy. pku. edu. cn / cgi - bin / ? protparam ), and the antigen epitope was predicated via software dnastar

    鄭州大學2002年碩士畢業論文人鈉h被匹協同轉運目白zk合蛋白抗體的制擊皿其在矚臟的這達研究方法：首先利用網際網路上軟體mp ： llexpasypku ? edu ? cn7cgl七inlprotparam ，分析蛋白理化性質和二級結構，應用生物技術軟體dnastar預測hnadc3抗原表位。
  17. A novel protein immobilization approach based on mercapto - pha sam for piezoelectric immunosensors has been proposed, which is formed by coupling mercaptoprorionic acid ( mpa ) to poly ( allylamine hydrochloride ) ( pha ) via 1 - ethyl - 3 - ( 3 - dimethylamino - propl ) carbodiimide ( edc ) and n - hydroxysuccinimide ( nhs ). after self - assembling a mercapto - pha sam on the gold electrode of the quartz crystal, the goat - anti - igm antibody is immobilized via cross - linking with glutaraldehyde. the detecting linear range of the piezoelectric immunosensor employing mercapto - pha sam is 0. 66 - 26. 4 ug / ml

    應用偶聯試劑碳二亞胺（ edc ）和n -羥基琥珀酰亞胺（ nhs ）使聚丙烯胺（ pha ）與巰基丙酸（ mpa ）偶合形成巰基化的聚丙烯胺，應用這一新方法，實現了羊抗人igm抗體分子的固定，並在0 . 66 26 . 4 g / ml范圍內對人血清免疫球蛋白igm進行了測定。
  18. Method to collect respectively 180 unrelated males " venous blood 500ul, who lived in shanxi province, 120 unrelated mongolians " venous blood 500ul, who lived in the inner mongolia autonomous region, and the blood is anticoagulant with edta, then to extract dna by using the method of phenol - chloroform after ingested by proteinase k at 56 and amplify the dys413 site by using pcr

    方法採集180例山西漢族和120例內蒙古蒙古族男性無關個體靜脈血各500ul ， edta抗凝，用tkml液反復洗滌至無色，加入2蛋白酶k緩沖液180ul ，蛋白酶k （ 20mg ml ） 20ul ，在56消化至液體清亮為止，用酚-氯仿法抽提dna ， pcr擴增dys413位點， 6非變性聚丙烯酰胺凝膠電泳， 1硝酸銀染色分型。
  19. 2. the putative epitopes that displayed on phages were identified by indirect elisa using swine antisera against prrsv and mouse antisera to recombinant structural protein of prrsv. seven putative epitopes could be recognized by antisera

    利用prrsvbj - 4陽性血清和鼠源抗重組結構蛋白抗體，採用間接elisa方法對噬菌體展示的表位進行了鑒定。
  20. A dot - ppa - elisa method has been developed to detect the antibody in swine serum against pasteurellosis. the capsule polysaccharide and outer membrane protein of pasteurella multocida was prepared as the diaphragm antigen

    本研究提取豬巴氏桿菌菌株的莢膜和外膜蛋白抗原，首次建立了檢測豬巴氏桿菌病血清抗體的dot - ppa - elisa方法。
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