融合溶菌 的英文怎麼說

中文拼音 [rónggěróngjūn]
融合溶菌 英文
confluent lysis
  • 融 : Ⅰ動詞1 （融化） melt; thaw 2 （融合; 調和） blend; fuse; be in harmony Ⅱ形容詞[書面語]1 （長遠; ...
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 溶 : 動詞（溶化; 溶解） dissolve
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 融合 : fuse; mix together; anastomosing; reconcile; harmonize; compromise; amalgamate; coalesce; coalesc...
  1. The contents of this studies include : 1 ) according to the researches on the correlation between the function and structure of the cmiv from bombyx - moxi before by others, especially by lixinlal in naigin normal university of china, we have designed and sythesized the mutation i of the gene of cmiv that was different from the natural cmiv about 50 % in amino sequence, using the favorable condon of the ecoli. after cheked the result of synthesis by sequence, we have cloned the gene into 3 " of the gene of thioredoxin in the thio - fusion expression vector ( ptxfus ), and the fusion protein of thio - cmiv was highly expressed in soluble form

    本研究的內容包括：一、在前人對抗菌肽cmiv研究的基礎上，對n端和c端進行氨基酸保守變換，設計和合成了該基因，充分使用大腸桿菌偏愛的密碼子，並將該基因5端與硫氧還蛋白基因3端融合，通過ptxfus表達載體獲得較高可溶性表達（在15 sds - page膠上可見明顯的表達蛋白帶） 。
  2. Renaturation of thermolabile hemolysin inclusion bodies expressed by vibrio parahaemolyticus

    融合表達的副溶血弧菌不耐熱性溶血毒素包涵體復性的研究
  3. Its content was about 9. 8 % among total cell protein by gene genius bio imaging system. the fusion proteins were found largely in an insoluble inclusion bodies. the purified fusion proteins was obtained by his6 technique used to immunize rabbits to obtain polyclonal antiserum with titer of 1 * 105

    經工ptg誘導，重組質粒在點co力『 blzi中表達出了c端融合了6xhis的融合蛋白，過量表達的蛋白主要以不溶性蛋白形式存在，其表達量占菌體總蛋白的9 . 8 % 。
  4. The reults of sds - page and western blot dermonstrated that the insoulable component of the induced e. coli culture contained protein 3a. the results of the study indicated that protein 3a existed in the form of inclusion body. the content of the expressed protein in the induced bacteria protein was 29. 2 % and 22. 1 % respectively

    Sds - page和westernbolt結果證實，大腸桿菌菌體不可溶性蛋白中富含3a蛋白，且此融合蛋白的分子量符合預期設計，說明3a蛋白在表達產物中以包涵體的形式存在，所表達的蛋白含量分別占菌體蛋白的29 . 2和22 . 1 。
  5. The engineered strain ghd - yz26 / e. coli bl21 ( de3 ) was incubated and induced at 30 for 8 hrs. the results show that the target fusion protein is soluble and active, which is about 15 % compared with the total proteins in cells, and the enzymatic activity reaches 5 u / ml that is about 8 folds compared with the activity of strain yz - 26

    Colibl21 （ de3 ）中30誘導培養8hrs ，可獲得可溶性表達，融合蛋白的表達量約占總菌蛋白的15 ，其酶活力為5u ml ，約是原始菌株的d -海因酶表觀活性的8倍。
  6. 2 ) basis of upon studies, we have also designed and sythesized the mutation ii of the cmiv that been greatly changed in the 3 " of the gene comparing with nature cmiv : the ht gf3 ( the third loop region of htgf2 specifically binding to egfr receptor ) was fused to 3 " of gene of cmiv through a flexible linker. the gene of the mutation ii of cmiv was sequenced and clonged to the vector of ptxfus to fuse to the 3 " of gene of thioredoxin

    二、在以上研究的基礎上，對cmiv抗菌肽的c端進行較大的改造，即將與腫瘤細胞過度表達的表皮生長因子受體（ egfr ）具有高親和性的因子多肽tgf _ 3通過疏水柔性接頭連接在抗菌肽cmiv的c端，設計完整的基因，並在大腸桿菌中利用ptxfus表達載體與硫氧還蛋白進行可溶性融合蛋白表達。
  7. Lymphotoxin ( lt ) is a kind of pleiotropic lymphocyte - secreted cytokine which mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. in order to increase the antitumor activity of lymphotoxin and reduce its side effects, the recombinant plasmid pet36b - lt 27 was constructed to express soluble fusion protein cbd - lt 27. the active form of lt 27 could be collected directly with several simple steps by three kind of components on the expressed fusion protein

    本研究通過構建表達n端缺失27個氨基酸的淋巴毒素融合蛋白的重組質粒，在大腸桿菌中實現融合蛋白的可溶及分泌表達，同時利用表達載體上的幾種特殊序列經簡單的分離純化步驟直接獲得大量的有生物活性的淋巴毒素缺失體lt 27 ，為尋找一種高抗腫瘤活性、低臨床毒副作用的生物抗癌藥物進行了有效的探索。
  8. The soluble fusion proteins cbd - lt 27 in cytoplasm and periplasm of e. coli were purified with cbind 100 resin, the purity of fusion protein was more than 80 % in eluted solution

    採用cbind100resin從破菌上清中快速純化可溶的融合蛋白cbd - l7a27 ，洗脫下來的融合蛋白純度可達80以上。
  9. The expressed fusion protein occupied more than 20 % of total bacterial protein. the fusion protein induced mainly existed in the insoluble inclusion body of the cell, only little parts of fusion protein expressed in the cytoplasm, excreted into periplasm and secreted into the cultural medium as soluble protein. through ultrasonic treatment at low temperature, soluble expressed fusion protein could be obtained from the supernatant of lysed cell

    表達產物在細胞內外的精細定位研究表明，融合蛋白cbd - lt 27在經誘導的大腸桿菌中表達量占總菌體蛋白的20以上，融合蛋白主要以不溶性包涵體的形式存在於細胞中，少量以可溶產物的形式存在於細胞質、分泌于細胞周質間腔及培養基中。
  10. All above showed that peacl preserved the polymerization activities of actin, and gfp fusion gave facilities for the study of microfilaments proper ties in vitro. monomer his - tagged peac1 - gfp could notably inhibit dnase i activities

    上述結果表明，通過gfp的融合而在大腸桿菌中可溶性表達的peac1 ，不僅很好保持了肌動蛋白的聚合活性，而且gfp的熒光特性也方便了微絲體外特性的研究。
  11. Though the expression level of bl21 ( de3 ) / pt221 - hyuh was lower than that of m15 / pqe60 - hyuh, the target protein of bl21 ( de3 ) / pt221 - hyuh was principally in soluble form while the objective protein of m15 / pqe60 - hyuh was principally in insoluble form. both of the products in the two strains showed biological activity, but the former is 2 times higher than the latter. the hyuc dna fragment was inserted into ppic3. 5k plasmid to construct the ppic3. 5k - hyuc recombinant plasmid which was then transduced into pichia pastoris gs115 cells after being linearized by bgl ii digestion

    結果表明， sds - page分析在50kd處有一較強的表達帶，融合有分子伴侶的重組菌株bl21 （ de3 ） pt221 - hyuh與非融合表達的重組菌株m15 pqe60 - hyuh相比，乙內酰脲水解酶的表達量低一些，但其表達蛋白主要以可溶性形式存在，而m15 pqe60 - hyuh中表達蛋白則主要以包含體形式存在，且前者的酶活性是後者的2倍多。
  12. We find that the fusion proteins are inclusion bodies when the bacteria lysised by the lysozyme. the product of pet - 32a ( + ) - igf - i was analyzed by western blotting, which confirmed that the hybrid protein was expressed as expected

    菌體採用溶菌酶裂解，融合蛋白以包涵體形式存在；對higf -融合蛋白進行了westernblot分析，確認所表達蛋白為目的蛋白。
  13. One potential drug could interfere with the proteins ' activity in a way that would force the entry vacuole to fuse with lysosomes, triggering an immune attack right after the chlamydiae invade the cell

    其中一種的可能作用，在於干擾這些蛋白質的活性，迫使入侵囊泡與溶小體融合，而在披衣菌侵入細胞后，即迅速引發免疫攻擊。
分享友人
分享到 Line

分享到臉書