融液出 的英文怎麼說
中文拼音 [róngyèchū]
融液出
英文
taspout-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。And original european critical parts, ensure our products safe, reliable, comfortable and quiet. it is installed easily without pit and just needs home appliance power supply. elegant design and refine craftwork make the house more graceful as well as reflect taste of successful life
秉承通力環保節能的設計理念，融合當今最新機電液技術，符合歐洲標準機械產品導則98 37ce ，原裝歐洲關鍵部件，確保了產品安全可靠靜音舒適無底坑220v電源，安裝方便設計雅緻，工藝精美，為家居增添便捷與華麗，折射出成功的生活品位，是豪華私宅高檔別墅中時尚而必備的新概念家用電器。This dessertation systematically researched dislocation and ab microdefects etched by molten koh and by ab etching respectively in gaas substrate, which were provided by merchants from home and overseas, as well as their influence on electrical parameters
本文系統地研究了國內外各生產廠家提供的gaas襯底中用熔融koh腐蝕的位錯、 ab腐蝕液腐蝕出的ab微缺陷的密度和分佈情況以及它們對襯底電參數（電阻率、遷移率、載流子濃度）的影響。Through study of correlative contents of advanced computer cybernetics, artificial intelligence, the domain knowledge and special crop growth mechanism in greenhouse, we present the system of multi - sensor data fusion ( msdf ) based on radial basis function network ( rbf ) to implement on line detection for nutrient - liquid, which may realize multiple components detection on - line, for example no3 -, cl -, ca2 +, ph, ec, nh4 +, k + and so on. the soft sensor ' s mechanism is introduced to overcome the limitations of sensor ' s manufacturing process. to improve the believe - degree of soft sensor ' s result, we analyze soft sensor ' s result by uncertain inferential capacity and combination rule of evidential theory
本論文通過對計算機技術、控制理論、人工智慧技術和設施農業領域知識等相關理論的研究，結合對特定溫室蔬菜生長的研究與機理分析，提出了一種基於rbf神經網路的營養液多傳感器數據融合（ msdf ）系統，實現對營養液組分： no _ 3 ~ - 、 cl ~ - 、 ca ~ （ 2 + ） 、 ph 、 ec 、 nh _ 4 ~ +和k ~ +的在線檢測；對于由於目前傳感器製造工藝的限制而不能在線檢測的離子成分如磷酸根和硫酸根，提出了一種基於徑向基函數網路的軟測量機制，可以有效地實現對營養液中磷酸根和硫酸根成分的實時檢測；為了提高軟測量結果的可信度，利用d - s證據理論的不確定推理能力和合成公式，結合領域知識對軟測量結果進行可信度分析。Calculated the aggregation frequency for the crown - shaped liquid droplets on condersor at > 90 in light of the properties of liquid droplets distribution function, and the detailed balance equation is derived for liquid - vapour phase transition
摘要根據冷凝壁面液滴分佈函數的概念和性質，計算了> 90時冷凝壁面球冠形液滴的融並頻率；導出純氣液相變下冷凝壁面的細致平衡方程。The research on the effect of liquid - liquid transition of polyacrylate on crystallization - melting of pdms indicates that above the melting temperature of pdms, pdms is reinforced by a special mutual action between p - electron in carbonyl of polyacrylate and 3d orbit in si atom of pdms, while below the temperature, the pdms crystal is lessened, which can not completely remove the crystal of pdms. however, it is the crystallization behavior of pdms that results in its most segments relaxation being restrained
研究聚丙烯酸酯液一液轉變對聚二甲基硅氧烷結晶一熔融影響的結果表明：碳基碳原子上p電子與出原於中3d軌道相互作用，在聚h甲基硅氧烷熔融轉變溫度以上，對其有分子增強作用；在聚二甲基硅氧烷熔融轉變溫度以下，霎對其有降低結晶度的作用，但不能消除結晶現象。The liquid infiltration - extrusion technology, as a new forming method which includes the liquid forging and the solid extrusion, has been developed in recent years. the process in essence is that after liquid metal is infiltrated into the fiber perform under pressure, high pressure is directly applied to it so that the melted metal is to be crystallized and solidified under pressure, and then undergoes large plastic deformation at the liquid - solid state or the state just solidified
液態浸滲擠壓是近年來發展起來的一種融液態模鍛和固態擠壓為一體的新成形工藝，其工藝實質是使液態基體金屬在壓力下滲入纖維預制體中，發生壓力下結晶凝固，並在液-固態或剛凝固態經受大的擠壓塑性變形，一次成形出金屬基復合材料的型材類製件。Western blot analysis we treated 200 fertilized eggs in a series of steps including lysing them in l0ul of lysis buffer ( 50mm tris - hcl ph 7. 5, 250mm nacl, 5mm edta, imm dtt, 0. 1 % triton, 50mm sodium orthovanadate, looug / mg pmsf and tpck, 50ug / ml tlck, 1 ug / ml leupeptin pepstatin and aprotinin ), frozing them below - 70, and then thawing them at room temperature for 10 min
2 . westem印跡將處理組及對照組小鼠一細胞g2期受精卵各200個取出，轉移到ep - pendoff管中， 5000甲m離心4分鐘，棄去上清，加入ro川粉碎緩沖液，充分振蕩混勻，在液氮中經過2一3次的凍融循環，迫使卵細胞裂解，加人等量的樣品緩沖液沸水中煮5分鐘，用於westem印跡分析。Furthermore, the egfp - eo gene was also cloned into pci - dhfr, a cho ( dhfr " ) cell expression vector. following the transfection and mtx selection, some cho cells presented green fluoresence, which will establish a good foundation for production of soluble eo glycoprotein and investigating its biological functions
將p2種子液以moi5 - 10接種指數期sf9細胞，三天後呈現出最強的熒光。我們還將egfp - eo融合基因插入哺乳動物細胞表達載體pci - dhfr中，構建重組質粒pci - dhfr - egfpeo 。Theoretically, the firstly, when explained the special phenomenon about financial structure during chinese reform, the financial structure concept of goldsmith had faced all sorts of confusion ; the secondly, the theory of financial structure ’ s evolution rule nearly relies on analytic method by " bs and ms " model, neglecting the approach to the microscopic mechanism inherent laws about the financial structure. in the practice, firstly whether the supplies of fund can continues or not is very important, because it is the safeguard to realization of reform ; secondly, finance in reform can satisfy the new request by completing other functions during the future transformation
理論上，首先在解釋中國轉型時期出現的金融結構特殊現象時，戈式的金融結構概念出現了種種的困惑，其次，有關于金融結構演進規律的理論幾乎都依賴于「直、間接融資比例與構成」分析法，忽略了對金融結構微觀機制的內在規律進行研究;實踐中，首先是資金的供給能否持續，這是對轉型實現的血液供給;其次，金融在轉型期是否只是完成資金的供給作用，在後轉型時期經濟對金融結構發展的新要求是什麼，現有的金融體系能否給予滿足，這是轉型成功的持久動力保障。The approximate analytical solution of melting process within a spherical enclosure is to presented by contacting heat transfer. a numerical model describing the melting process is presented by considering the difference of the solid - liquid density and natural convection
在所建立的模型中考慮了固相與液相的密度差和自然對流的影響，得出了各個時刻液膜厚度的分佈、融化速率和傳熟量等計算結果。To solve the combinatorial optimization problem of outer layout and inner connection integrated schemes in the design of hydraulic manifold blocks ( hmb ), an intelligent virtual design method was proposed with combining modern intelligent optimization methods with virtual design technology
摘要為解決液壓集成塊設計中外部布局和內部布孔集成的組合優化問題，提出了一種融合現代智能優化方法和虛擬設計技術的智能虛擬設計方法。If we adopt the advantages of two forming methods simultaneously, we can present a new forming method that is liquid combination process of die casting and forging
如果兩者融合，揚長避短，實現雙控，就可以提出一種新的成形方法，即液態鑄鍛雙控成形。This study we acquired the coding region of hcv ns5b gene by pcr of hcv full length genome and construct the recombinant plasmid pegep n3 - ns5b ; with the different concentration of g418 in the culture medium, we think the selection concentration of g418 for hepg2 cell is 800 g / ml ; the recombinant plasmid was transfected into hepg2 cell by lipofectamine2000 cells containing stable transformants were selected by the ability of resistance to g418 and isolated with the limited dilution. the stably transfected cell line expressing ns5b - egfp fusion protein was obtained by the detection under fluorescence microscope and rt - pcr
本研究首先從hcv基因組中擴增出nssb基因，構建了nssb基因與報告分子egfp （增強型綠色熒光蛋白）基因的融合基因真核表達質粒pegfpn30 ；通過含有不同g418濃度梯度的培養液培養hepgz細胞，確定了篩選用g4181作濃度為800pg ml ；利用脂質體法將該重組質粒轉染hepgz細胞，經過有限稀釋法和g4壓力選擇，應用熒光顯微鏡和rtpcr檢測，獲得可穩定表達nssbegfp融合蛋白的hepgz細胞克隆。To validate fault diagnosis method using data fusion integrated ann and evidence theory in pump fault diagnosis, this paper analyzes the common fault mechanism of piston pump, brings forward diagnosis domain, gives diagnosis
為了驗證神經網路和證據理論集成的數據融合診斷方法用於液壓泵診斷的有效性，本文分析了柱塞泵常見故障機理，提出了液壓泵的診斷域，建立了診斷的模型，並對診斷網路的結構進行了構造。分享友人