血清轉化 的英文怎麼說
中文拼音 [xiěqīngzhuǎnhuà]
血清轉化
英文
seroconversion-
Finally 4 right clones was obtained from 864 transformants by pcr detection. sequencing analysis showed that the hsa gene have been introduced into the right position of the human a - lactalbumin yac. furthermore, works to optimize the conditions of introducing the recombinant yac into goat f ibroblast via cell fusion was explorded
經pcr檢測，從864個轉化子中獲得了4個陽性克隆，測序表明，人血清白蛋白基因已正確的導入到人-乳白蛋白基因yac的特定位點上，並獲得了可進行細胞融合的重組yac載體。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。The result showed that the homology rate of pila gene among the 5 avian pathogenic e. coli strains tested and one human e. coli were from 89. 8 % to 91. 1 %, and the homology rate of amino acid were from 88. 5 % to 91. 8 %. the homology rate of pila gene sequence among 5 avian pathogenic e. coli strains tested and avian pathogenic e. coli reported ( serotype o1, o2, o78 ) were from 87. 8 % to 90. 2 %, and the homology rate of amino acid were from 84. 6 % to 91. 2 %. there had homology in avian pathogenic e. coli. there had some common antigen side in type 1 pili of avian pathogenic e. coli
結果表明：運用msha法檢測1型菌毛的檢出率為80 （ 36 45 ） ， pcr法的檢出率為95 . 5 （ 43 45 ） ， pcr方法用於1型菌毛的檢測比msha更加敏感、快速、特異性強；選擇5株優勢血清型雞源致病性大腸桿菌代表株（ o _ （ 89 ） ， o _ （ 119 ） ， o _ （ 141 ） ， o _ （ 127 ） ）的1型菌毛pila基因的pcr擴增片段經純化后，分別定向克隆到puc18質粒的多克隆位點，構建了含有目的基因片段的克隆質粒，並轉化到dh5株大腸桿菌載體菌中，篩選獲得陽性克隆菌株。Change of serum transferrin in children with positive hemoculture
血培養陽性敗血癥患兒血清轉鐵蛋白變化Aminated and hydroxylated polysulfone membranes were prepared by amination and hydroxylation reaction, respectively. then bovine albumin ( bsa ) - fixed membranes were obtained by crossed - linking albumin into porous membrane with 1, 1 ' - carbonyldiimidazole and bisoxirane reagents, respectively. a mathematical model for facilitated transport in asymmetric membranes with fixed site carriers was derived by assuming an instantaneous, microscopic concentraion fluctuation in the membrane
以氯甲基聚碸為基材，通過相轉化法制備出具有底部貫通孔的非對稱膜，通過胺化和羥基化反應，分別制得胺化聚碸膜和羥基化聚碸膜，再採用羰基二咪唑和雙環氧烷兩種活化試劑對其進行活化，將牛血清白蛋白固載在膜內，獲得固定白蛋白促進傳遞膜。After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company
實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。According to above consideration, experiments was carried out as below : first, targeted gene - human serum albumin ( hsa ) gene was obtained via pcr technology. secondly, the hsa gene was liganded with a plasmidprla22 whose two arms carry dna sequences necessary for crossing with the human a - lactalbumin yac to form an integration vector prla - hsa, then the integration vector plasmid was co - transformated into the right yac - bearing yeast with another plasmid plrh33 which carrys a selective gene
因此，本試驗首先擴增出整合在酵母基因組里的人血清白蛋白（ hsa ）基因作為目的基因，並將人血清白蛋白基因插入到一個含有人-乳白蛋白yac同源序列的重組型質粒載體，以構建整合型載體，再與另一個帶篩選基因的質粒共轉化入含人-乳白蛋白yac的酵母細胞體內。The mind is most active during the day, when it runs wild and is distracted, resulting in an increase in the amount of serotonin required by the nerve cells. during the night or meditation, when the mind is less active, less serotonin and more melatonin are produced, and the situation changes
白天時，人類有意識的活動極為活躍，心靈動蕩散亂，需要更多的血清張力素來供應神經細胞而到了晚上或靜坐時，情形就恰恰相反，有意識的活動變少了，因此有更多的血清張力素轉化為褪黑激素。In the first part of the present work, the expression changes of angiotensinogen ( agt ) and angiotensin - converting enzyme ( ace ) as well as its time course characteristics were investigated by rt - pcr, in situ hybridization and western blotting. we also prepared the polyclonal antiserum against agt for the following work
故本工作應用rt - pcr和原位雜交、 western印跡分析等方法對模擬失重大鼠不同部位動脈血管血管緊張素原（ angiotensinogen ， agt ）及血管緊張素轉化酶（ angiotensin - convertingenzyme ， ace ）基因表達變化的時程特徵進行了觀察，並為后續工作制備了抗agt多克隆抗血清。Melatonin production is inversely proportional to that of serotonin, a chemical that constricts the blood vessels and acts as a neurotransmitter
褪黑激素是由負責神經傳輸功能的血清張力素serotonin轉化而來。Methods : take the hemonlysin, phagocytic activity of peritoneal macrophages and lymphocyte transformation as the target to determine the immunity function
方法：以血清溶血素、巨噬細胞吞噬功能、淋巴細胞轉化功能為指標進行免疫功能的測定。Results : kuqigan granules can impromove the production of hemonlysin, enhanced the phagocytic activity of peritoneal macrophages, increased the transformation of lymphocyte
結果：苦芪甘顆粒具有促進血清溶血素生成、增強腹腔巨噬細胞吞噬功能、提高淋巴細胞轉化率的作用。( 6 ) pdgf may initiate neuronal differentiation in nsc. nsc induced by pdgf, transcription and translation level of different thrs genes were up - regulated compared with the nsc induced by serum. the results made a great foundation for the further research of the important role of thrs during the cns development, and have much clinical significance on therapy of cretinism ^ subcretinism a
Thr各亞型在不同分化細胞中表達不一致： （ 0l ；能誘導nsc向少突膠質細胞分化，並通過thr各亞型的時空表達來發揮其重要生理作用門印叩樸能引發vc向神經幾分化，與10血清誘導分化相比能在轉錄與翻譯水平上提高nsc分化后thr各亞型的表達本研究結果將為深入研究t在腦發育中的作用提供寶貴的實驗依據，並對克汀病、亞克汀病以及ms脫髓鞘病等的臨床治療提供新途徑，具有重要指導意義。Serous cortisol content in each group increased, while cortisol content in group i was higher than that in each other group ( p < 0. 05 ). serous - ep content in each group increased and then decreased gradually, but - ep content in group iv has a significant increased at half an hour following incision ( p < 0. 05 ). conclusions canine immune functions are suppressed by operation with different anesthetic techniques to different degree, but resume at 15th day after operation. there is an interacting and interadjusting mechanism among the cns ( central nerve system ), the endocrine system and the immune system
結果各組在麻醉手術后4h及術后1d白細胞總數、嗜中性分葉狀粒細胞數顯著增加（ p 0 . 05 ） ，淋巴細胞數減少；血清球蛋白含量先降低后升高；組rbc ? c _ 3br花環率先降低再升高后降低，其它組均先降低； t淋巴細胞轉化率均呈現不同程度的降低，在術后7d左右恢復正常；血漿皮質醇濃度先升高后降低，但組皮質醇含量在切皮后30min較其它組上升顯著（ p 0 . 05 ） ；血漿- ep濃度先升高后降低，但組- ep含量在切皮后30min較其它組上升顯著（ p 0 . 05 ） 。Methods : in cultured lung explants without serum, the lipid component synthesis of pulmonary surfactant was evaluated in [ 3h ] - choline incorporation ; mrna content of phosphocholine cytidylyltransferase ( cct ) in lung explants was investigated in rt - pcr ; the changes of the ultrastructure of the at ii cells were observed with electron microscope ; the expression of nmdar1 subtype was observed in immunohistochemistry staining ; nitric oxide synthase ( nos ) activity, nitric oxide ( no ) content, superoxide dismutase ( sod ) level, malondialdehyde ( mda ) content and lactae dehydroase ( ldh ) level were determined by biochemistry methods. results : 1. influence of glutamate on synthesis of the lipid component of pulmonary surfactant ? with l - arginine, glu inhibited [ 3h ] - choline incorporation with good dose - dependence and time - dependence ; ( 2 ) mrna content of cct of the glu treatment groups was decreased ; ( 3 ) glu increases the release of ldh in cultured lung explants ; ( dwith electron microscope histochemistry, glu induced the changes of the ultrastruture of at ii iv cells
方法：採用成年大鼠肺組織無血清培養，運用[ ~ 3h ] -膽堿摻入法測定ps主要脂質磷脂酰膽堿（ pc ）合成量； rt - pcr擴增檢測肺組織中pc合成限速酶磷酸膽堿二胞苷酰基轉移酶（ cct ） mrna含量；透射電子顯微鏡法觀察肺泡型上皮細胞和ps系統超微結構的變化；免疫組織化學染色檢測glu的受體nmdar1亞單位的表達；生化測定肺組織乳酸脫氫酶（ ldh ）釋放量和肺組織勻漿中一氧化氮合酶（ nos ）活性、一氧化氮（ no ）生成量、超氧化物歧化酶（ sod ）水平以及丙二醛（ mda ）含量。The overall number of seroconversions was relatively low in both groups : two of the 363 patients in the tenofovir arm seroconverted versus six of the 368 patients in the placebo arm
在兩組女性中，血清轉化病患總數都分別降低，服用泰諾福韋的363人中有2例，接受安慰劑的368人中有6例。The results found a 65 % decrease in hiv seroconversion in women who received tenofovir ( 0. 86 % per 100 person - years ) compared with women who received placebo ( 2. 48 % per 100 person - years ), although the difference did not reach statistical significance
結果表明，使用泰諾福韋的女性， hiv血清轉化率降低了65 % ，有一年0 . 86 %的人發生了轉化，而使用安慰劑的女性中，一年有2 . 48 %的人發生了血清轉化，盡管這個差別並沒有達到具有統計意義的要求。The highly effective prevention education that was given to study participants in both arms of the study may have resulted in lower rates of seroconversion than had been anticipated ( two in the tenofovir arm and six in the placebo arm ), decreasing the ability of the study to detect a significant difference in risk of hiv infection
對兩組參與者進行的極為有效的安全教育也有可能是血清轉化率低於預期效果的原因，從而降低了該研究探索泰諾福韋在預防hiv感染中和其他療法的差別的能力。Interferon is gens protein, - interferon calls leucocyte interferon again, it is to treat the remedy with chronic second liver, third most effective liver at present, it is reached through restraining virus to duplicate adjust immune function, make serum transaminase returns to normal, improve liver histology to change, prevent the happening of liver cirrhosis and liver cancer, achieve the goal that raises patient life quality and survival rate
干擾素是一族蛋白質， -干擾素又稱白細胞干擾素，是目前治療慢性乙肝、丙肝最為有效的藥物，它通過抑制病毒復制及調節免疫功能，使血清轉氨酶恢復正常，改善肝組織學變，防止肝硬化和肝癌的發生，達到提高病人生活質量和生存率的目的。分享友人