表生菌根 的英文怎麼說
中文拼音 [biǎoshēngjūngēn]
表生菌根
英文
extramatrical mycorrhiza-
It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity
本研究中,木耳屬2個種的2個菌株在its區域表現出較高的保守性, 4種限制型內切酶的酶切圖譜沒有顯示出多態性;增加內切酶種類及供試菌株數量,有可能獲得具有多態性的限制性內切酶酶切圖譜; 9本實驗中, its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異,擴增片段長度均為650bp左右; 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析,紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法,兩者的關系是相輔相成,互為驗證Genetic diversity and phylogeny of 55 slow - growing rhizobia isolated from peanut ( arachis hypogaea ) in china were determined by analysis of host - plant range, phynotype, 16s rrna rflp, 16s rrna sequence, 16s - 23s igs rflp, rapd, rep - pcr, dna - dna hybridization homology. at the same time, the competitive nodulation capacity of rhizobia, effect of host plants and soil ph on the rhizobia were determined for screening and improvement of high effective rhizobium inoculant
本研究採用宿主范圍試驗、表型性狀測定、 16srrna - rflp 、 16srrna序列分析、 16s - 23srdnaigsrflp分析、 rapd分析、 rep - pcr分析和dna - dna同源性分析等技術系統研究了從我國不同地域分離的55株花生根瘤菌的遺傳多樣性及其在根瘤菌系統發育中的地位和相互關系。The materials as explant in transformation come from birch leaf, stem segment and leaf stalk, and the spider toxin gene was used as foreign gene for this transformation experiment. it showed that the best explant was the big leaf, on which the transformation frequency was 22 %. by gus detection, there were 43 percent of the plants with kanamycin resistance, and 100 percent of positive result, by pcr amplification, was gotten from random sampling
利用雙元載體的根癌農桿菌lba4404菌株( agrobacteriumtumefaciens ) ,含質粒pyhy (目的基因及npt 、 gus基因) ,對白樺試管苗莖段,葉柄,葉片三種外植體進行侵染,結果表明:大葉片生長勢強,為轉基因的最優外植體,轉化率能夠達到22 。The survey shows that there found mycorrhizae in all the 7 tree species beside prunus ansu. through differentiating, we understand that these ectomycorrhizal fungi are 8 different species which are subordinated to 6 families and 7 genera
結果表明:除山杏未發現外生菌根真菌共生外,其它7個樹種上均發現了菌根真菌的共生現象。10 phenons at the similarity level of 80 %. the fingerprints of rep - pcr which conducted by boxair primer showed that the isolates from the root nodules of pueraria spp. had g reat genetic diversity. they were divided into 11 genetic clusters at the similarity level of 81 %
以boxair為引物的rep - pcr分析結果表明葛藤根瘤菌存在不同水平的遺傳多樣性,在81的相似性水平上,供試葛藤根瘤菌分成了11個遺傳群,其結果證明了慢生葛藤根瘤菌的遺傳多樣性。Results of host - plant range test shown that all slow - growing isolates could effectively form nodules on arachis hypogaea and phaseolns vulgaris. they could not nodulate on visia sativa, and part of them could nodulate glycin soja and glycin max
宿主范圍研究表明供試花根瘤菌均能在花生上進行有效的結瘤並固氮;部分菌株能夠在野大豆和栽培大豆矮腳早上結瘤;所有供試菌株均不能在野豌豆上結瘤,但均能能在菜豆上結瘤且單株瘤數較多。Multiplicity of signals and diversity of signaling pathways exist during the establishment of mycorrhizal associations together with the regulation of symbiosis - specific genes expression. this mechanism of signal recognition and transduction related with development process of the symbiont was reviewed at the molecular level
在菌根共生體建立過程中存在信號分子的多重性和信號通路的多樣性以及共牛體特異基因的表達調控,從分子水平上揭示了菌根整個發育過程。The tolerance of suillus granulatus and paxillus involutus to zn, cd, and pb was then compared using liquid culture medium without agitation. compared to suillus granulatus, paxillus involutus was more tolerant to zn and pb, but more sensitive to cd than suillus granulatus. the adsorption of heavy metals in ectomycorrhizal fungi might be related to the base exchange capacity of the fungal mycelium
在此基礎上,研究了兩種外生菌根真菌對不同濃度重金屬cd和zn的耐性能力,發現不同真菌對不同重金屬的耐性不同,表現在卷緣樁菇對zn的耐性高於點柄乳牛肝菌,而兩種真菌對cd的耐性則相反。Mongolica young stand, secondary natural forest, and the mixed forest of larix olgensis and fraxinus mandshurica rupr. countermeasures for controlling soil degradation were also recommended. the study got following results : ( 1 ) from young stand to mid - age of larix olgensis, with the closure of canopy and the decrease of undergrowth vegetation cover, soil acidity, enzyme activity, total inorganic phosphorus, available k, soil moisture and total porosity decreased significantly ; while soil organic matter content, humus component content, the amount of three main types of microbes, acid phosphatase activity, and bulk density in rhizosphere soil and non - rhizosphere soil increased with the increase of the amount of forest floor litter ; from mid - age stand to near mature stand, with the decrease of stand density and crown cover as well as the restoration of undergrowth vegetation after preliminary thinning, soil organic matter content, fulvic acid, exchangeable acidity, exchangeable aluminium, neutral phosphatase activity, total amount of inorganic phosphorus, the amount of actinomycetes, soil moisture, and total porosity in rhizosphere soil and non - rhizosphere soil apparently increased ; from near matu re stand to mature stand, soil activity acidity, organic matter content, humus component content, available phosphorus, total amount of amino acid, available k, soil enzyme activity, soil moisture, and total porosity in rhizosphere soil and non - rhizosphere soil decreased, but total phosphorus, soil acidity ( in particular, exchangeable aluminium ), and bulk density increased
結果表明: ( 1 )從落葉松幼齡林到中齡林,隨著林冠的郁閉和林下植被蓋度的急劇下降,林地土壤酸度、土壤酶活性、無機磷總量、速效鉀、土壤水分含量、總孔隙度呈明顯下降趨勢,而根際和非根際土壤有機質含量、腐殖質各組分含量、三大類微生物數量、酸性磷酸酶活性和土壤容重隨著凋落物的增加呈明顯上升趨勢;從中齡林到近熟林,由於撫育間伐,林分密度及郁閉度下降,林下植被蓋度逐步得到恢復,根際與非根際土壤有機質含量、富里酸、交換性酸、交換性鋁、中性磷酸酶、無機磷總量、放線菌數量、土壤水分含量、總孔隙度呈明顯的上升趨勢;從近熟林到成熟林,隨著林齡的增大,根際與非根際土壤活性酸、有機質含量、腐殖質各組分含量、有效磷含量、氨基酸總量、速效鉀、土壤酶的活性、土壤水分含量和總孔隙度呈下降趨勢,而根際與非根際土壤全磷含量、土壤酸度(特別是交換性鋁)和容重呈增加趨勢。The results showed that quantity of bacterium and four bacterial physiology groups was positively correlated with quality of illumination ; their quantity showed a reduced tendency with the reducing of the illumination condition, but quantity of fungi was negatively correlated, it was increased gradually with the reducing of the illumination condition ; rhizosphere soil of kentucky bluegrass turned into fungi type from bacterium type ; the rhizosphere effect of various bacterial physiological group of kentucky bluegrass is obvious under different quality of illumination
結果表明,草地早熟禾根際細菌及四類細菌生理群數量與光照條件呈正相關,隨著光照條件的減弱,其數量呈降低趨勢;根際放線菌數量隨光照的減弱呈先下降後上升的趨勢;而真菌數量與光照條件呈負相關,隨著光照條件的減弱,根際真菌的數量逐漸增加;草地早熟禾根際土壤由「細菌型」向「真菌型」轉化;不同光照條件下,根際各微生物類群都表現出明顯的根際效應。The aim of this research is to establish a transgenic system for flower plants and investigate the functions of mapkk in transgenic plants in the future
本研究構建了野生型、突變型mapkk基因植物表達載體,並用凍溶法將其導入了根癌農桿菌lba4404 。Were studied together with the reference strains of recognized rhizobium and bradyrhizobiwn species by performing polyphasic taxonomy, including numerical taxonomy, rep - pcr fingerprinting, 16s rdna pcr - rflp. the result show that : the growth rate of rhizobia isolated from the root nodules of pueraria spp. showed great diversity. ccbau41147 ccbau6110 k ccbau61096 and ccbau61095 were fast - growing strains, the single colony size was bigger than 1mm after 2 days incubated oq yma medium at 28 they can produce acid. the other strains were slow - growing strains, their single colony size was less than 1 mm after 7 days incubated on yma medium at 28. they can produce alkali
本研究以從我國四川、河南、安徽和湖南等地分離的32株葛藤根瘤菌為研究對象,以20株已知種的根瘤菌為參比菌株,採用數值分類、 rep - pcr指紋分析、 16srdnapcr - rflp指紋分析等現代根瘤菌分類技術,初步研究了葛藤根瘤菌的生物多樣性和分類地位,結果表明:葛藤根瘤菌在生長速率上表現出多樣性,菌株ccbau41147 、 ccbau61096 、 ccbau61101和ccbau61095生長較快, yma培養基上28培養2 - 3天後,單個菌落直徑大於1mm ,具有產酸能力,是快生型葛藤根瘤菌;其餘待測葛藤根瘤菌生長較慢, yma培養基上28培養7天後,單個菌落直徑小於1mm ,具有產堿能力,是慢生型葛藤根瘤菌。5. the morphology of the two strains also shows that the flagella of ss - ori are around the cells, whilst the strain yz - ii6 has only a single flagellum grown at the polar of cells besides the different of cell size, though they both are bacillus and gram - negative
形態和生理生化特徵也表明, ss - ori菌周生鞭毛,革蘭氏反應呈陰性, yz - 6菌為極生單根鞭毛,革蘭氏反應呈陰性。Biodiversity exists among am fungi and is influenced by numerous factors including soil properties and plant species. if am fungi are to be used in sustainable agricultural systems it is necessary to study native am fungi in the target areas and then select efficient isolates that can be applied as inocula in the field to improve crop growth. the objectives of this study were to investigate the germplasm of am fungi, to understand the distribution pattern of am fungi in different ecological conditions such as area, soil factor and host plant, to select isolates effective in nutrient acquisition by the host plant sweet potato, to test their effectiveness under field conditions, and to monitor amf after their introduction into the field
本研究通過調查我國北方部分地區的am真菌資源,研究了am真菌的種群組成及其在空間、土壤利用方式和宿主植物類型等不同環境條件和空間尺度上的分佈規律;在此基礎上,根據它們對甘薯的生長、吸磷效應篩選出高效菌株,在大田條件下研究了am真菌菌絲的分佈特性、代謝活性及其對甘薯產量和品質的影響;並通過分子探針跟蹤調查了引入am真菌在共生體中的發育和表達,以期為菌根真菌的生產應用提供技術支持。Results of g + c mol % test shown that all slow - growing isolates were belong to the same species. more than 70 % dna - dna homologies were determined among 4 representative strains usda6, and usda110 ( type strains of b. japonicum ). low dna homologies were detected with usda76 ( type strain of b. elkanii )
中l習花生根瘤菌遺傳多樣性和系統發育研究g + cmol %和dna一dna同源性分析結果表明,供試花生根瘤菌代表菌株的tm和g + cmol %均小於種內變異l隔度,表明供試花生根瘤菌均屬j二一個種。In this paper, bioremediation technology of contaminated soil with organic pollutants was summarized, which were technologies of the microorganism remediation, the phytoremediation and the mycorrhiza remediation for contaminated soil, the future developments of these bioremidation technologies were prospected as follows : when the efficient surfactants was used to enhance phytoremediation systems, its optimum doses should be considered ; studying on the important role of the rhizosphere exudates in the phytoremediation, seeking the best matching partners between plant and microorganism and the best mycorrhizal funguses to improve pollution degradation
摘要綜述了有機污染土壤生物修復的三種技術,即微生物修復技術、植物修復技術、菌根生物修復技術及其研究現狀,並展望了這三種生物修復技術今後的研究方向,如利用表面活性劑提高植物修復效率時,應考慮其最佳使用量;加強研究根分泌物在植物修復土壤污染中的作用;進行植物微生物聯合體篩選技術研究;篩選促進污染物降解的優良菌根菌種等。Concentrations of zn and cd were significantly lower in mycorrhizal plants, but p concentrations did not differ substantially between mycorrhizal and non - mycorrhizal treatments. the results indicate that the higher metal tolerance of mycorrhizal plants was due to the direct involvement of the mycorrhizal fungi in plant tolerance to heavy metals rather than a p - mediated nutritional effect
通過盆栽試驗研究了接種外生菌根真菌對油松幼苗耐重金屬能力的影響,結果表明在重金屬zn和cd污染條件下,點柄乳牛肝菌侵染油松后,顯著改善了植株生長狀況,大幅度降低了植株體內zn和cd含量,而菌根與非菌根處理植株磷營養差異不大。A hypothesis was suggested as following : firstly, the host plants could not synthesize enough carbohydrate to meet their own growth requirements and those of the fungus when the plant in experiment conditions of severe p deficiency. as a result, extraradical hyphal growth and am fungal metabolic activity were inhibited and therefore decline
宿主植物磷營養狀況較差時,菌根真菌生長和代謝活性旺盛;宿主植物磷營養狀況較好時,菌根真菌生長和代謝活性受到抑制,表明宿主植物的磷營養狀況對am真菌的生長和代謝活性具有調控作用。Abstract : quorum - sensing is a physiologic accommodation of bacterial in gene expression according to its quorum
摘要:群體感應是細菌根據細胞密度變化進行基因表達調控的一種生理行為。Combining the analysis of the conservative region of 16s rrna gene of prokaryote, such as e. coli, rhizobia and several frankia strains, we designed several sets of primers to amplify the 16s rrna gene of the frankia strains tested. through tentative experiments with these primers, we screened out primers uf / ur and ec27f / frl717r
通過比較已發表的原核生物,如大腸桿菌、根瘤菌和弗蘭克氏菌的16srrna基因全序列的保守區,設計了8對引物並篩選出可適用於擴增13株供試菌株16srrna基因接近全長序列的引物: uf ur和ec27f fr1717r (產物大小約1500bp ) 。分享友人