表面酵母 的英文怎麼說
中文拼音 [biǎomiànjiàomǔ]
表面酵母
英文
top yeast- 表 : Ⅰ名詞1 (外面;外表) outside; surface; external 2 (中表親戚) the relationship between the child...
- 面 : Ⅰ名詞1 (頭的前部; 臉) face 2 (物體的表面) surface; top 3 (外露的一層或正面) outside; the ri...
- 酵 : 動詞(發酵) ferment; leaven
- 母 : Ⅰ名詞1 (母親) mother 2 (泛指女性長輩) one s elderly female relatives 3 (配套的兩件東西里的凹...
- 表面 : surface; superficies; boundary; face; rind; sheet; skin; outside; appearance
- 酵母 : yeast酵母浸液[提取液] yeast extract; 酵母聚糖 zymosan; 酵母片 aluzyme; 酵母細胞[植物] yeast plant; yeast cell
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Uses : used in the manufacture of persulfate ; used in the manufacture of aniline dyes ; used in the treatment of oxidized metal surface ; used as the initiator for the polymerization of monomers ; used in decoloring and bleaching of oil, cleaning and deodorization of deteriorating barm, accelerating ripening agent ; used as additive for under - well fracturing in petroleum mining
用途:用於製造過硫酸鹽的製造;用於苯胺染料的製造,甕染料的氧化發色劑;用於氧化金屬表面處理;用於有機合成工業中,高分子物聚合的引發劑;油的脫色和漂白,變敗酵母的洗滌,脫臭,小麥的催熟劑;石油開採用于井下壓裂的添加劑。Display of the envelope protein of d2 - 43 on yeast cells surface
蛋白在酵母細胞表面的展示Pas stain reveals the budding cells and pseudohyphae of candida on the surface of the tongue
Pas染色顯示舌表面上假絲酵母的芽殖細胞和假菌絲。A pas stain reveals the budding cells and pseudohyphae of candida on the surface of the tongue
Pas染色顯示舌表面上假絲酵母的芽殖細胞和假菌絲。The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides
進一步對xynba進行了脫糖基化處理得到xynbb ,其分子量恢復到23kd ,證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現:三種酶的最適ph差異不大, xynb和xynba均為5 . 2 , xynbb為5 . 0 ; xynb和xynba的最適溫度均為60 , xynbb降為50 :在耐熱性上, xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ; xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ,明顯低於原酶的比活2814 . 45iu mg ; xynb和xynba的km值相當,分別為21 . 56 ( g kg )和20 . 87 ( g kg ) ,而xynbb的km值較大為27 . 10 ( g kg ) ; xynba和xynbb的vmax相差不大,分別為4568 mol mg ? min和5329 mol mg ? min ,明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性,對胃蛋白酶和胰蛋白酶有很好的抗性,且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現:以樺木木聚糖為底物時,酶解產物主要為木三糖和木四糖,含量分別為68 . 43和16 . 50 ,另外還含有11 . 79的木二糖;以玉米芯木聚糖為底物時,酶解產物主要為木二糖和木三糖,含量分別為81 . 78和11 . 55 。Then cdnas were labeled with cy5 or cy3 respectively by incorporating cy5 - dctp or cy3 - dctp into the reaction during reverse transcription of rna
採用cartesian基因晶元列印儀,將純化的cdna片段列印在corning玻片表面,製作了酵母細胞基因表達譜晶元。[ size = 5 ] [ color = darkred ] 38. [ / color ] [ / size ] [ / b ] the hot sun had caused the dough ( 面團 ) to double in size and the fermenting yeast ( 酵母 ) made the surface shake and sigh as though it were breathing and it looked like some unknown being from outer space
炙熱的太陽導致面團面積加倍,酵母使面團的表面擺晃嘆息,似乎它在呼吸,它看上去像某種來自外部空間的無名生物。Abstract : plant responses to salt stress via a complex mechanism, including sensing and transducing the stress signal, activating the transcription factors and the corresponding metabolizing genes. since the whole mechanism is still unclear, this review emphasize the biochemical events during the plant adaptation to salt stress referring to an index of importance : the homeostasis in cytoplasm, the biosynthesis of osmolytes and the transport of water. most of these biochemical events were elucidated by study of halophyte and salt - sensitive mutations, also many important genes involved were cloned and used to generate stress - tolerance phenotypes in transgenic plants. on the other hand, about the molecular mechanism in signal transduction, the research of arabidopsis mutations and yeast functional complementation provided helpful traces but not full pathway
摘要植物對鹽脅迫的耐受反應是個復雜的過程,在分子水平上它包括對外界鹽信號的感應和傳遞,特異轉錄因子的激活和下游控制生理生化應答的效應基因的表達.在生化應答中,本文著重討論負責維持和重建離子平衡的膜轉運蛋白、滲調劑的生物合成和功能及水分控制.這些生理生化應答最終使得液泡中離子濃度升高和滲調劑在胞質中積累.近年來,通過對各種鹽生植物或鹽敏感突變株的研究,闡明了許多鹽應答的離子轉運途徑、水通道和物種特異的滲調劑代謝途徑,克隆了其相關基因並能在轉基因淡水植物中產生耐鹽表型;另一方面,在擬南芥突變體及利用酵母鹽敏感突變株功能互補篩選得到一些編碼信號傳遞蛋白的基因,這些都有助於闡明植物鹽脅迫應答的分子機制。Yeast surface display technology and its applications
酵母表面展示技術及應用This paper reviews the recent advances in yeast expression systems used in recombinant protein drugs produced by gene engineering, such as the selection of yeast vector system and yeast expression strain, construction of multi - copy strain, the high yeast cell density culture, the recent situation of yeast system applied in expression gene, defect of yeast expression system and countermeasure, etc
摘要本文主要從酵母載體系統的選擇、酵母表達宿主的選擇、多拷貝菌株的構建、高密度發酵培養酵母細胞、應用酵母系統表達外源基因的研究現狀、酵母表達系統的缺陷及對策等幾個方面綜述了近年來酵母表達體系在基因工程重組蛋白藥物開發方面的研究進展。Expression of hgm - csf in bombyx mori cells and larvae of silkworms has been successful, but it ' s quantity of expression is much less than bmnpv
近年來,在gm - csf基因工程研究方面得到了迅速發展,如在哺乳動物細胞、酵母、大腸桿菌、昆蟲細胞中都得到了成功表達。分享友人