親和層析法 的英文怎麼說

中文拼音 [qīnhécéngxīfǎ]
親和層析法 英文
affinity chromatography
  • 和 : 和動詞（在粉狀物中加液體攪拌或揉弄使有黏性） mix （powder） with water, etc. : 和點兒灰泥 prepare some plaster
  • 層 : i 量詞1 （用於重疊、積累的東西 如樓層、階層、地層） storey; tier; stratum 2 （用於可以分項分步的...
  • 析 : Ⅰ動詞1. （分開; 散開） divide; separate 2. （分析） analyse; dissect; resolve Ⅱ名詞（姓氏） a surname
  • 法 : Ⅰ名詞1 （由國家制定或認可的行為規則的總稱） law 2 （方法; 方式） way; method; mode; means 3 （標...
  • 親和 : affine親和嫁接 congenial graft; 親和數 amicable number; friendly number
  1. After the protein refolding of denaturant inclusion body following dialysis, we got the pure recombinant gst - eo protein by gst affinity columns. using the purified protein as coating antigen, an indirect elisa were developed for detecting the anti - eo antibody in the csfv serum by exploring the concentration of coating an tigen and dilution degree of serum

    使用分步透析法對變性的包涵體進行復性，將復性蛋白過gst親和層析柱得到純化的gst - eo融合蛋白。以gst - eo融合蛋白為診斷抗原，初步建立了用間接elisa檢測豬瘟血清eo抗體的方法。
  2. Determination aflatoxins content in food - cleanup by immunoaffinity chromatogaphy and determination by high - performance liquid chromatigraphy and fluorometer

    食品中黃麴黴毒素的測定免疫親和層析凈化高效液相色譜法和熒光光度法
  3. Determination of aflatoxin m1 content in milk and milk powder - cleanup by immunoaffinity chromatogaphy and determination by high - performance liquid chromatigraphy and fluorometer

    乳和乳粉中黃麴黴毒素m1的測定免疫親和層析凈化高效液相色譜法和熒光光度法
  4. Purified fusion protein gst - hnadc3 was used as an immunogen to inoculate rabbits and the antibody against the gst - hnadc3 fusion protein was raised, and was purified by gst sepharose 4b affinity chromatography to remove the antibody against gst

    Ptg誘導表達，超聲破碎細胞后，採用親和層析方法純化融合蛋白gst十nadc3 ，並以此為抗原免疫紐西蘭株白兔制備融合蛋白抗體。應用親和層析的方法對gst十nadc3融合蛋白抗體進行純化，以去除抗gst抗體。
  5. Conentional methods such as fractionation of complex clinical samples by ionic exchange chromatography and new methods such as enriching low abundant proteins by affinity capture with a combinatorial library of ligands14 proide much needed tools for processing complex biological and clinical samples for proteomics research

    傳統（如對復雜臨床樣品通過離子交換層析進行分離）和現代的方法（如通過親和捕獲與配體庫的組合富集低豐度蛋白）為處理用於蛋白質組學研究的復雜的生物學與臨床樣品提供了極需的工具。
  6. In order to detect the effect of human sperm mannose - ligand receptor on the fertilization ability, in the study reported here mannose - ligand receptors ( mrs ) were purified from human sperm by modified mannose - agarose gel affinity chromatography coloumn and determined protien concentration by lowry, preincubated zona - free hamster oocytes with four purified mannose - ligand receptor ( pmr ) concentrations before sperm penetration assay ( spa ) to test the pmrs cell biology nature of inhibition to fertilization

    本研究用改良后的親和層析法分離純化mr ， lowry法測定其蛋白質濃度，在精子穿透試驗（ spermpenetrationassay ， spa ）模型中定量研究其對精卵融合能力的影響並檢測其細胞生物學活性；以已知濃度的pmr （ purifiedmannose - ligandreceptor ）干預精子半透明帶試驗，觀察用pmr預處理半透明帶對精子與透明帶結合的影響。
  7. The dna encoding the desired protein was generated as a bamh i - sal i fragment using pcr. the template was pbv220 - hgf - a strand that had been constructed in our lab

    其中gst是一種純化標簽，可以在後續的蛋白分離純化中使用gst親和層析的辦法純化目的蛋白，這將使純化工作變得簡單而有效。
  8. Study on purification of rhepo by the immunoaffinity chromatography

    免疫親和層析法純化重組人促紅細胞生成素的實驗研究
  9. The recombinant protein exhibited specific binding to antiviral antibodies of h5 subtype and has no cross - reaction with h7 and h9 subtype

    分別用親和層析以及電洗脫兩種方法對gst - ha1融合蛋白進行了純化，都獲得了理想的純化結果。
  10. Purification, identification of human telomerase and its protein subunits

    親和層析法分離鑒定人類端粒酶復合體及其蛋白質組分分析
  11. The ascites fluid antibody purified by 33 % ammonium sulfate is further purified by chromatography. the p24 affinity chromato - graphic column done to purify the bispecific antibody is in the brcn activated sepharose 4b. after washing the unspecific proteins, change the washing buffer and the anti - p24 / anti - human rbc type a bispecific antibody and the anti - p24 monospecific antibody are gained respectively

    方法是先用硫酸綏鹽析，再過親和層析柱，即33飽和硫酸按沉澱后，透祈過夜，上p24抗原做配體的濱化氰活化的sepharose4b柱，流洗掉雜蛋白和抗人紅細胞單特異性抗體后，再洗脫並收集雙特異性抗體。
  12. In order to investigate the role of mannose receptor ( mr ) of human sperm, the zona free hamster eggs were pre - incubated with purified mr ( pmr ) isolated from motile human sperm by mannose - agarose gel affinity chromatography. the ultrastuctural alteration and cortical granule exocytosis of the eggs were then observed by transmissian electron microscope and tritc - lca immunofluorescence microscope, respectively. the mice were immunized with pmr and the antiserum was raised. after capacitation and induction of the acrosome reaction, the human spermatozoa and oocytes were incubated with the antiserum. then the sperm penetration assay was undertaken

    為了進一步探討人精於mr在精卵融合中的作用，本文採用改良后的甘露糖-瓊脂糖凝膠親和層析法分離純化人精子mr ，並將提純的人精子甘露糖受體（ purifiedmannosereceptor ， pmr ）作用於去透明帶的金黃地鼠卵母細胞，運用透射電子顯微鏡技術和羅丹明偶聯的兵豆凝集素（ tritc - lca ）免疫熒光標記技術觀察pmr對卵子的影響。
  13. Methods : the phosphorylated proteins from human kidney tissues were enriched with phosphate metal affinity chromatography ( pmac ) resin

    方法：利用金屬磷酸鹽親和層析樹脂富集人腎組織磷酸化蛋白。
  14. This paper is composed four parts. first part, purpose and significance of the study, research situation and method will be described. second part, descriptive study the behavior code of conduct of the peasants in city. find out that behxior code of conduct has threecharacters. first, change takes place in approachs of cognition which is mainly composed with communication media rather than consang and rative

    第二部分對進城農民行為規范再社會化的狀況進行了描述性分析，發現進城農民行為規范再社會化表現出三個方面的特徵，一是在認知的途徑方面，進城農民已改變過去依靠血緣、親緣為主的途徑，逐步轉向傳媒化的途徑為主；二是在日常生活規范、工作規章制度、法律規范三今方面，進城農民在認知、遵守和認同三個層次表現出一定的差異性。
  15. Although hippocampal neurons and astrocytes also showed positive binding, cerebellar granule neurons are the more promising binding source because of their high yield, good binding and biological relevance. in the second part, prp was constructed into a recombinant protein with human igg fc fragment ( prpfc ) and used directly to interact with its bindin

    再次，在受體的鑒別和分離中，改進傳統的親和層析方法，以多功能交聯劑連接配體和受體，並分別以streptavidin及proteina偶聯的磁珠為結合反應載體，省lo以酉i
  16. Methods : the mouse pem gene ( mpem ) cdna coding sequence was cloned into prokaryotic gst fusion protein expression plasmid pgex - 4t - 3. the recombinant plasmid was transformed to e. coli bl21 and the gst / mpem fusion protein was induced to express with iptg. the fusion protein was purified by affinity chromatography

    方法： pcr擴增小鼠pemcdna編碼序列，將它克隆到含有gst的原核表達質粒載體pgex - 4t - 3上，轉化大腸桿菌bl21 ，誘導表達gst mpem融合蛋白，通過親和層析，獲得初步純化的產物，以羥胺切割驗證其一級結構。
  17. The large - scale expression product is concentrated and desalted by ultra - filter, and the target protein is separated by affinity chromatography with chitin, and then cut by cnbr. the purified product appears evident antibacterial activity with method of agar diffusion

    採用優化的表達條件進行大量發酵，對上清液超濾濃縮脫鹽后，採用幾丁質基質進行親和層析分離目的蛋白，溴化氰切割，瓊脂孔穴法測抗菌活性。
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