試劑盒 的英文怎麼說
中文拼音 [shìjìhé]
試劑盒
英文
test kit-
Methodological evaluation of homemade reagent for afp with time - resolved fluoroimmunoassay
時間分辨熒光免疫分析試劑盒的方法學評價Viral rnas were extracted from virus - infected allantoic fluids using qiaamp mini - extraction kits. after reverse transcription, cdna was amplified using specific primers for each gene segment
用qiagen的rneasyrna試劑盒提取病毒rna后，逆助興才李醫『筍脂碩聖『老『才轉錄合成cdna 。; sabc and tunel kit were from boster co., ltd. 3
Sabc和tunel染色試劑盒購于中國bos幾r公司。Development of the dipstick of gold - immuno chromayography assay for the diagnosis of plague
鼠疫膠體金法快速診斷試劑盒的研製Value of three kinds of tuberculous antibody in diagnosis of tuberculosis
三種結核抗體診斷試劑盒對結核病的診斷價值In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company
實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。In this article, we describe methodologies needed inmanufacturing the immunoassay kit from the preparationof each component to the stabilization of thesystem constructed
在這篇文章中，我們描述了製造免疫檢測試劑盒所需的方法學，從各種成分的準備到構造成的系統的穩定。Pgem - t vector system, jm109 competent cells, reverse transcription kit and in vitro translation kit were purchased from promega company
Pgem一t載體，感受態細菌jm109 ，反轉錄試劑盒和體外翻譯試劑盒均購自promega公司。Study and manufacture radioimmunoassay kit of norgestrel
18甲基炔諾酮放射免疫分析試劑盒的研製Extraction of large - fragment genomic dna in order to gain dna template of pcr amplification ( long pcr amplification and salvage pcr amplification ) which was high purity and large fragment, three methods were used to extract genomic dna of bacillus subtilis, i. e. low melting - point agarose embedding method, sds - proteinase k - phenol chloroform extraction method and bacterial genomic dna extraction kit method. the genomic dna of bacillus subtilis were gained by these methods, and the operated programs of the methods were improved. the results showed that the genomic dna extracted by low melting - point agarose embedding method were obviously biggest than that of another two methods
大片段基因組dna的提取為了獲得用於pcr擴增（長距離pcr擴增和分段pcr擴增）的高純度、大片段（至少為pcr產物長度的4倍）的dna模板，應用三種方法：低熔點瓊脂糖包埋法， sds -蛋白酶k -酚氯仿抽提法和細菌基因組dna提取試劑盒法，分別提取獲得了枯草桿菌基因組dna ，並對3種方法的操作程序進行了不同程度的改進，結果表明：低熔點瓊脂糖包埋法提取的基因組dna片段明顯大於后兩種方法，採用0 . 5瓊脂糖凝膠電泳3h ，仍然跑不出加樣孔。Since there are more things in the bud tissue of the male flower, such as phenolic compounds, polysacchrides, proteins and some other secondary metabolites, three methods used to isolate rna are tested in this assay, which are the enzyme digesting methods of ctab, ctab - dna and sds - dna
克服了常規方法和試劑盒無法提取出富含酚類化合物、多糖和一些尚無法確定的次級代謝產物的白樺花芽組織rna的障礙，提出了3種白樺雄花芽組織rna的提取方法： ctab 、 ctab - dna酶消化法和sds - dna酶消化法。Constructing cdna expressing library of erythrocytic plasmodium falciparum from hainan : the total rna was obtained by using. triplix kit. a modified oligo ( dt ) primer ( cds ffi pcr primer ) was used in the single - stranded ( ss ) dna synthesis reaction. the ss - dna was reversely transcripted from total rna. double - stranded ( ds ) cdna was amplified by long - distance ( ld ) pcrafter the digestion with proteinase k and sfi i, the cdna with no less than 200bp was collected and purified by glass - milk kitthe library was constructed after the ligation of cdna to tiplex2 phage particle packaged with the packaging extract system in vitro. a high titer and high recombinant ratio of cdna library was constructed
構建惡性瘧原蟲海南株紅內期cdna表達文庫提取紅內期惡性瘧原蟲海南株總rna ，直接以總rna為模板使用cdna文庫構建試劑盒，首先反轉錄合成ss一dna ，再擴增合成ds一dna （ cdna ） ，對擴增產物用蛋白酶k消化及左z丁i酶切，抽提蛋白、去除rna后，用玻璃奶試劑盒純化、回收20obp以上的片斷，經與載體連接再用蛋白包裝物包裝后形成未擴增文庫，最後擴增完成惡性瘧原蟲海南株紅內期cdna表達文庫的構建。Methods : 1 ) 12h after irradiation, the cell cycle of nih3t3 cells was determined by flow of cytometry and the ratio of alternations in p16 gene exon - 2 was evaluated through pcr - sscp. 2 ) the content of mda, the activities of the sod and gsh - px in the supernatant of nih3t3 cells and the cells were measured by detecting kits immediately after irradiation. 3 ) the level of matrix metalloproteinase - 2 ( mmp - 2 ) in hela cells was detected by western - blotting and dot - blotting 2h after irradiation
具體方法為： （ 1 ）照射后12h ，收集nih3t3細胞，用流式細胞儀檢測各組細胞的細胞周期， pcr - sscp檢測抑癌基因p16的變化； （ 2 ） nih3t3細胞照射后立即收集細胞和細胞上清，用試劑盒測量mda含量和sod 、 gsh - px的活性並觀察其變化； （ 3 ） western免疫印跡和點雜交法檢測照射2h后的各組hela細胞中基質金屬蛋白酶- 2 （ mmp - 2 ）的表達變化。In the experiment, the first instar housefly larvae " metallothionein mrna expressing reach maximur. after induced by cd ( 2mm ) for 48 hours, then total rna was extracted with guanidinium isothiocyanate, and mrna was isolated by the polyattract mrna isolation systems surplied by promega company
試驗中採用2mmcd ~ 2對家蠅初孵幼蟲誘導48小時，使其體內金屬硫蛋白mrna表達達到高峰，在液氮中將其研磨成粉末后採用異硫氰酸胍法提取總rna ，再用promega公司提供的小量分離mrna試劑盒純化mrna 。It can be futher verified by the test result that the pstvd found in the northeast region of china may be introduced into china by following the introduction of the potato cultivar irish cobbler to various countries and irish cobbler was imported in heilongjiang province prior to the 1960s
在國內，首次對中國分離株系進行cdna基因克隆和序列分析，進一步驗證了中國株系的由來及其結構特點，對于中國在pstvd檢測水平的提高、檢測試劑盒制備、 pstvd的防治及其致病機理、轉基因育種等提供重要依據。After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence
F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。At the same time, 10 serum sample were detected by elisa kit made in abroad and agp. the result of three elisa detection shows : s / p of 8 detected serum higher than 0. 2 is positive ; s / p of 2 detected serum is negative. the result accords with the result of agp detection
最後，再將自製vp1板用自配試劑檢測其活性，同時用自製板和國外試劑、國外elisa試劑盒、瓊脂擴散試驗檢測同樣10份血清作比較，三組elisa檢測結果，其中有8份被檢血清s p （抗體水平）大於0 . 2 ，為陽性； 2份被檢血清為陰性。Comparison of elisa and two rapid immuno assays for the detection of chlamydia trachomatis in urogenital specimens
金標快速免疫試劑盒檢測沙眼衣原體的性能評估Inspection criteria of the quality of clinical chemistry in vitro diagnostic kits. general guideline
臨床化學體外診斷試劑盒質量檢驗.總則In fact, the marketexpansion of home - version diagnostic kits in developedcountries, typically in the united states, far exceedsthe average for overall in vitro diagnostic products
事實上，家庭診斷試劑盒在發達國家的市場擴張，以美國為代表，遠遠地超過了全部體外診斷產品的平均值。分享友人