誘發酶 的英文怎麼說
中文拼音 [yòufā]
誘發酶
英文
inducible enzyme （= induced enzyme）-
A study on apoptosis and activity of sapk jnk in adrenocortical cells induced by cadmium chloride
氯化鎘誘發腎上腺皮質細胞凋亡與應激活化蛋白激酶活性的研究Abstract : several - aromaticamino ketones, which were designed according to some hypothetical models of the cyclooxygenase and 5 - lipoxygenase active sites, were synthesized by an amino exchange reaction. the structures of the eight new compounds were confirmed by ir, 1h - nmr and elemental analysis. the results of the pharmacological tests showed some of the investigated compounds had significant anti - inflammatory activity on croton oil - induced ear edema of mice
文摘:根據環氧化酶、 5 -脂氧化酶活性中心結構模型設計了一組-芳胺酮類化合物，並用胺交換反應合成了這些化合物.經紅外光譜、核磁共振氫譜及元素分析證實了8個未見文獻報道的化合物的結構.藥理實驗結果顯示.部分受試化合物在巴豆油誘發小鼠足趾腫脹模型中表現出一定的抗炎活性In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。Niger with phytase activity about 2250 u / ml which selected by protoplast - uv mutation was used as original, prepared it into fungu - suspend - liquid, through uv mutation, daub on the filter - substract. pre - primary - selection was according to the lucency - circle, primary - selection was one fungus inoculate one flask to ferment, secondary - selection was using several high phytase activity fungus through one fungus inoculate 2 - 3 flasks to ferment. then one or two high phytase activity fungus of the secondary selection were used in the next mutation cycle
首先用粗略制備的原生質體經紫外誘變篩選到一株酶活為2250u ml的實驗出發菌；制備成菌懸液，紫外燈下照射誘變，紅光下塗抹篩選平板，恆溫培養2 3d ；按透明圈大小進行預初篩，挑選徑圈比大的菌落接斜面，恆溫培養3d ；按一株一瓶的方式進行初篩；從中選取酶活較大的3 5株，按一株2 3瓶的方式進行復篩；挑取酶活大且穩定的1 2株進入下一代誘變篩選。Based on the extensive studies of subtilisin - like protease ( prl ) of metarhizium anisopliae, extracellullar serine protease is suggested to be a key enzyme involved in the fimgal penetration to invertebrates. the investigation of serine protease in the nematode infected by owvtl may help to understand the mechanism of nematophagous fimgi as biological control agents. a 3l kda serine protease was isolated and purified from the liquid culture of h rhossiliensis owvtl challenged with nematode panagrellus redivivus
本研究利用線蟲誘導下owvt - 1菌株液體發酵，通過粗分級分離、離子交換層析和凝膠過濾層析分離提純了一個分子量為31kda的絲氨酸蛋白酶，生物學測定表明其對大豆胞囊線蟲二齡幼蟲具有致死作用，同時測定了該酶理化特性，酶活力在75附近酶活力最高，隨著ph的增加酶的穩定性升高，與膽堿酯酶具有相似的ph曲線，對特異性底物aape （ suc - ala - ala - pro - glu - pna ）具有作用， ssi和ci - 2抑制該酶的活性。Conclusion a systematic method for preparation of enzyme - mannanse is established, a high productive strain was got after seducing and selecting from nature, confirmed as brachybacterium spa6 research were conducted on medium and culture method of the strain in order to get the suitable cultural condition of fermentation, the experiment result shows the optimium condition is ph7. 0, temperature 36c ; carbon content 2. 5 %, ventilation in abundence, agitation speed 200r / min
結論1 、以從自然界中篩選出的菌株為出發株，經誘變、篩選，得一高產葡甘聚糖酶菌株，初步鑒定為短桿菌屬brachybacteriumspa6 2 、經誘變、三角瓶培養，該菌株的最適培養條件：培養基ph值7 . 0 ，碳源2 . 5 ，振蕩培養， 200r min ，培養溫度36 ，培養48h 。Through screening a lot of mutants with the method of transparent zones and culture filtrate, the best four were obtained with high - yield of stable phb depolymerase, named as 02, 04, 09 and 14
以青黴（ penicillium . sp ） ds9701為出發菌株，通過紫外線誘變分生孢子，採用透明圈初篩和搖瓶培養復篩的方法，獲得4個能穩定遺傳的phb解聚酶高產菌株。No, a first gas information molecule discovered in human being, is a typical endothelial - derived relaxant and mediates endothelium - dependent relaxation of blood vessels. in the pathogesis of endotoxin shock vec is one of the major target cells of lps and lps - induced proinflammatory cytokine such as tumor necrosis factor and interlukin 1 and activated. in vec inducible nitric oxide synthase ( inos ) is induced and lead to an increase in production of no, the while endothelial nitric oxide synthase ( enos ) is inhibited and elicit decrease in no formation, both of which are demonstrated to induce the
在內毒素休克過程中vec是lps及其誘導機體產生的多種促炎細胞因子如tnf 、 il - 1作用的主要靶細胞， vec誘導型一氧化氮合酶（ induciblenitricoxidesynthase ， inos ）激活、 no大量誘生而內皮型一氧化氮合酶（ endothelialnitricoxidesynthase ， enos ）活性被抑制、 no生成障礙，是血管反應性異常變化、血管調節機制紊亂的重要發病環節。Studies suggest that it probably interacts directly with the viral polymerase, and we want to confirm that by mutagenesis and by enzymatic activity assays, " said tao
有研究表明，核蛋白可能會和病毒的聚合酶直接發生相互作用，我們將通過人工誘變技術及酶活性測定等技術來核實這一說法。 」In the present study, in order to investigate the effects of endogenous estrogen on the daergic terminals in amy and the daergic neurons in midbrain, fast cyclic voltammetry ( fcv ) was used to examine da release evoked by electrical stimulation from amy of female rats in different phases of estrus cycle, ovx rats and male rats. and tyrosine hydroxylase ( th ) immunohistochemi stry was employed to measure the numbers of daergic neurons of ventral tegmental area ( vta ) and substantia nigra ( snc ) in the rats
因此，為進一步探討大鼠內源性雌激素水平的變化對amyda能神經系統及中腦da能神經元的影響，本工作應用快速周期伏安法（ fcv ）在體監測了處于動情周期各期雌鼠、 ovx鼠和雄鼠經電刺激誘發的amyda釋放，並應用酪氨酸羥化酶（ th ）免疫組化方法測定了以上各組大鼠腹側背蓋區（ vta ） 、黑質（ snc ）的th陽性神經元數目。Specific pichia clony pcr product showed that foreign phytase gene was integrated into the host cell. the experimental results from flask fermentation and phytase activity assay indicated that phytase gene was effectively expressed by the recombinant pichia
挑選轉化子經過bmgy搖瓶培養、 bmmy誘導發酵后，用釩鋁酸按法測定了表達產物的酶活性，結果表明重組菌株可有效表達具有生物學活性的植酸酶。Detection of the effects of collagenase on spinal nerve conduction velocity of rats by using evoked potential
應用誘發電位測定膠原酶對大鼠脊神經傳導速度的影響As a proteasome inhibitor, bortezomib has shown inhibition on cell proliferation, inducing cell apoptosis, inhibition of cell adhension and inhibition of tumor angiogenesis for multiple myeloma cells
摘要硼替佐米作為一種蛋白酶抑制劑，對多發性骨髓瘤細胞表現出抑制細胞生長、誘導腫瘤細胞凋亡、抑制細胞粘附、抑制腫瘤血管生成等作用，同時對血液系統其他惡性腫瘤具有顯著的作用。In order to enhance the trehalose production, y7 was used for mutation with ems. through primary screening and compound screening, strain l61 with high - yielding trehalose and deep neutral trehalase activity was screened
實驗對出發菌株y7進行了ems誘變處理，通過初篩、復篩，從中選育出一株能高產海藻糖同時具有較低海藻糖分解酶活性的菌株l61 。In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein
經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。Study on screening a high - productive strain of dextranase and its basic conditions of flask fermentation
葡聚糖酶高產菌株的誘變篩選及其搖瓶發酵條件的初步研究Phopholipase c - 1 ( plc - 1 ) is widely known to play an important role in regulating cell proliferation and differentiation, development of the organisms, cell transformation and oxidative stress. till now, the mechanism how phopholipase c - 1 acts can not be thoroughly illustrated, nor has the interaction between plc - 1 pathway and other signal pathways been systematically reported. this research chose 2 - de + ms as the basic method from all kinds of proteomics strategies and compared the total protein expression map of mef genetically deficient in plc - 1 ( plc - 1 - / - ) to that of wild type mef ( plc - l + / + ) aimed to find some protein spots differentially expressed, thus we can discuss the impact of knockout of plc - 1 on signal transduction initiated by growth factors such as egf comprehensively. in this way, we can study the biological function of plc - 1 and mechanism of plc - 1 pathway indirectly, which will contribute a lot to further analysis
鑒于plc - 1發揮上述作用的機制尚未完全闡明， plc - 1通路與其他信號通路間的交聯和代償尚無系統報道，又因為以往的研究方法不夠全面，本研究以野生型小鼠胚胎成纖維細胞（ plc - 1 ~ （ + / + ） ）和缺失磷脂酶c - 1的小鼠胚胎成纖維細胞（ plc - 1 ~ （ - / - ） ）為研究模型，在眾多蛋白組學策略中選擇了雙向電泳+質譜（ 2 - de + ms ）作為研究手段，通過對比表皮生長因子（ egf ）刺激24小時後上述兩種細胞的總蛋白質表達差異，全面地探討敲除plc - 1對生長因子誘導的信號傳遞的影響，從而間接研究plc - 1生物學作用、信號傳遞機制及其代償情況，為后續的深入研究打下基礎。Role of telomerase in chrysotile induced malignant transformation of normal human embryonic lung fibroblasts
溫石棉誘發人胚肺細胞惡性轉化中端粒酶的作用Effects of ubiquitin proteasome inhibitor induced endoplasmic reticulum stress and apoptosis on dopaminergic neuron
泛素蛋白酶體抑制劑誘發多巴胺能神經元內質網應激反應及其凋亡的作用- agkistrodotoxin ( - agtx ) is a presynaptic neurotoxin. with the methods of enzyme - linked fluorescence measurement and amino acid assay the experiments examined the action of - agkistrodotoxin on kcl depolarization evoked glutamate ( glu ), aspartate ( asp ), glycine ( gly ) and ? - aminobutyric acid ( gaba ) release from the isolated cerecortical synaptosomes, and its effect on the depolarization of synaptic membranes was also investigated
本文以分離的大鼠大腦皮層突觸體為材料，採用酶聯熒光測定和氨基酸分析的方法，檢測了- agtx對kcl去極化誘發的鈣離子依賴性谷氨酸（ glu ） ，天冬氨酸（ asp ） ，甘氨酸（ gly ）和-氨基丁酸（ g分享友人