讀碼框 的英文怎麼說

中文拼音 [dòumǎkuàng]
讀碼框 英文
orfs
  • 讀 : 讀名詞（語句中的停頓） a slight pause in reading
  • 碼 : Ⅰ名詞（表示數目的符號或用具） a sign or object indicating number; code Ⅱ量詞1 （指一件事或一類的...
  • 框 : 框名詞（框架; 框子） frame; case
  1. In our experiment, the specific fragment was amplified from transgenic bobwhite genome dna at annealing temperature 61 by using high - fidelity pfu dna polymerase and cloned into clone vector pgem - 7fz ( + ), then sequenced. the cloned sequence was completely identical to the sequence which was issued in genbank

    本實驗採用了高保真pfudna聚合酶，在退火溫度61條件下從轉基因bobwhite品種基因組dna中擴增出特異性片段，將此片段插入克隆載體pgem - 7fz （ + ） ，經測序和序列分析表明，所擴增得到的片段含有bar基因完整的讀碼框，並且序列與genbank中發表的序列完全一致。
  2. It ' s meaningful to study the function of rab proteins in ciliates and further to explicit the mechanism of vesicular trafficking. the rob gene was amplified from macronuclear dna of euplotes octocarinatus. the size of gene was 783 bp long with an orf of 624 bp encoding eorabl protein and containing three in - frame tga codes

    本研究利用pcr技術從游仆蟲（ euplotesoctocarinatus ）大核dna中擴增出rab基因，並對該基因進行序列分析，該基因全長為783bp ，兩端為端粒序列，編碼框為624bp ，編碼207個氨基酸，開放讀框中有3個tga ，在此編碼半胱氨酸。
  3. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  4. Two positive clones were sequenced, and the results showed that its nuclcotidc sequence includes an open reading segment which codes for a 45 - amino acids protein and three endonuclcase sites which arc1 bgii, bamh i and bgi ii, this protein was identified as metallothionein based on its characteristic described above and its similarity ( 85 % ) to the mtn gene of drosophila : the 10 cysteine residues present occur in five pairs of cys - x - cys, x is serine, valine, ilistidine or lysine

    結果顯示：擴增的cdna片段長度為289bp ，其中含有一個編碼45個氨基酸的開放閱讀框，閱讀框所編碼的氨基酸中含有10個半胱氨酸，且在序列中均排列成cys - x - cys ，其中x為ser 、 val 、 his或lys 。這些特徵說明擴增的基因片段為家蠅mt基因序列的一部分。此基因序列片段與果蠅mtn基因序列的同源性達到85 . 0 ，擴增的基因序列中含有三個內切酶位點bg 、 bam和bg ，這一點也和果蠅mtn基因十分相似。
  5. The start reading framae and stop codons, base composition in protein - coding genes and the codon usage of amino acids in scolopendra multilane were compared with the three other myriapods

    本研究在蛋白質編碼基因起始閱讀框和終止密碼子、蛋白質編碼區的堿基組中文摘要成、氨基酸及密碼子的利用等方面把少棘蜈蚣與另三種多足類進行了比較。
  6. In this study, we at first aimed at obtaining the gene encoding the specific ige antibody related proteins by immunoscreening the schistosoma juponi cum adult worm cdna library with the pooled high - titer ige antisera from the individuals living in the schistosome epidemic regions

    J測定。 jnij序結果顯示，該插入片段為1200hp ，第一開讀框長507hp ，編碼169個氨基酸殘基，理論分子量為19 3kdi 。
  7. It will provide us to further study the function of xanthophyll cycle in photoprotection. the major results are as following : two cdna sequences encoding violaxanthin de - epoxidase were cloned from japonica rice ( jrvde ) and indica rice ( irvde ) with the full - length of 1887bp and 1647bp, respectively. the homology of the open reading frame is 98 % identity between two rvde genes, and more than 60 % identities with those of other species

    本論文從水稻和菠菜中克隆了編碼vde酶的基因，並通過轉基因植物進一步研究了葉黃素循環在熱耗散方面的作用，主要獲得了以下結果：首次從兩個水稻亞種（秈稻和粳稻）中克隆了rvde基因（分別命名為irvde和jrvde ）的全長cdna序列，分別長1647bp和1887bp ，兩者開放閱讀框的同源性為98 ，與其它已知vde基因的同源性在60以上。
  8. The results of sequencing showed that jl94 isolate complete gene 6 was 1356bp and had a complete open reading frame which encoded 397 amino acides

    對克隆的vp6基因進行序列測定，測序結果顯示jl94vp6基因全長1356bp ，含有完整的開放閱讀框架，編碼397個氨基酸。
  9. This sequence emergences fourteen times from 1000 ests library indicts that it is a middle affluently gene in cdna library. the cdna of 634 basepairs contains an open reading frame of 339 nucleotides encoding a novel nonspecific lipid transfer protein. the first 23 amino acids constitute the putative signal peptide, characteristic for targeting to the secretory pathway

    測得th - nsltp序列全長為634bp ，含有一個非特異性脂轉移蛋白與植物耐逆性的相關性研究編碼112個氨基酸的閱讀框架， n端的23個氨基酸組成一段信號肽序列，表明它可能和分泌有關。
  10. Badh cdna ( 1901bp ) included a 66 bp 5 " utr, a 329 bp 3 " utr and a 1506 bp orf encoding a 501 - ammo - acid polypeptide which showed 88 % sequence identity to badh from spinach, sugar beet and atriplex hortensis respectively. the deduced amino acid sequence included a decapeptide sequence " vtlelggksp ", which is highly conserved among general aldehyde dehydrogenases ( aldh ), and a cysteine residue

    Badhcdna全長1901bp ， 5端非編碼區66bp ， 3端非編碼區329bp ，含有2個可能的加polya信號： aataa ，開放閱讀框架1506bp ，編碼一個由501個氨基酸構成的多肽，與菠菜、甜菜、山菠菜badh的氨基酸序列同源性均為88 ，其中有醛脫氫酶的保守序列vtlelggksp和半胱氨酸殘基。
  11. The result of the agarose gel electrophoresis showed that the length of the full - length cdnas in the library was pooled mainly between 500 and 2 000 base pairs

    結果表明獲得的ejoi基因的cdna長度為876hp ，開放閱讀框長度為759hp ，編碼252個氨基酸。
  12. The full - length cdna sequence was finally generated by 5 ' race and 3 ' race respectively. at the same time, structure and function of ejol gene were primarily analyzed by bioinformatics method

    結果表明獲得的ejo3基因的cdna長度為1514hp ，開放閱讀框長度為1368hp ，編碼456個氨基酸。
  13. The hcv genomic rna contains a single open reading frame and the non - code region of both laterals related to the replication and translation

    Hcv基因組全長約9 . 6kb ，含有一個單獨開放讀碼框，兩側為與復制和翻譯相關的非編碼區。
  14. One positive clone was obtained from the libraries with casein flat and electrophoresis comparison method. analyzed the sequencing result, the positive clone include the open read frame of mlap gene and upper regulatory sequence

    序列測定分析表明，此重組質粒包含有長度為1377bp低溫堿性蛋白酶基因的完整的開放讀碼框架（ orf ）和上游基因調控序列。
  15. One gene cassette usually contains a open reading frame encoding antibiotic resistance and a specific recombination site called attc ( or 59 - base element )

    基因盒往往由一個編碼抗生素抗性的開放讀碼框（ orf ）和一個整合位點attc組成。
  16. But there is no report on study of stress tolerance of dreb1c, which contains a single open reading frame of 216 amino acids and encodes a putative protein with a predicted molecular mass of 24. 3kd

    Dreb1c包含一個單獨的216個氨基酸的開放讀碼框，推導其所編碼的蛋白質分子量為24 . 3kd ，但對其抗逆性的研究尚未有報道。
  17. In this pathway, irel, an er transmembrane protein kinase / endoribonuclease, is essential for viability during er stress. in contrast to yeast, the mammalian genome contains two homologues of ire1, irelctand ire1b, to sense the unfolded protein level

    該基因的開放讀碼框（ openreadingfiame ， orf ）為1383bp ，編碼蛋白的大小為50kd ，大腸桿菌ibeb基因編碼的50kd前體蛋白經分子內剪切加工形成大小為34kd的成熟表達產物，定位於細胞外膜。
  18. We sequence the inserted gene fragment of the indentified recombinant clone. the result is : angiostatin gene orf ( open reading frame ) links with the orf in expression vector correctly. but the first base of the codon aaa coding for lys414 in plg kringle 4 domain mutates from a to g which leads lys change to glu

    隨后取通過上述鑒定的重組克隆菌，對重組子插入片段測序，結果為： as基因開放讀碼框與表達載體的讀碼框正確匹配相連，但在其kringle4區相當于編碼plg的lys ~ （ 414 ）密碼子aaa的第一位堿基由a突變為g ，導致相應的氨基酸殘基突變為glu 。
  19. A new e. coli promoter - probe vector phn1005 was constructed by using a red - shift enhanced gfpmut3 as reporter gene and showed the following characters : 1, bamhi at the 5 " end of gfpmut3 structure gene could be used to clone promoter - active fragment and the strength of promoter could be quantitatively assayed. 2, a rrnbtlt2 terminator from rrna gene at the 3 " end of gfpmut3 could permit clone strong promoter. 3, another rrnbt1t2 terminator was inserted upstream bamhi to prevent reading through of promoters from puc19

    進一步以紅移且熒光強度提高21倍的gfpmut3為報告基因，構建了大腸桿菌啟動子探針載體phn1005 ，該載體上gfpmut3結構基因5 』端的bamhi位點可用來克隆具有啟動子活性的dna片段並定量分析插入的啟動子強度；其3 』端含rrnat1t2終止子，可允許克隆強啟動子；在bamhi上游同樣插入rrnat1t2終止子以防止載體puc19上的啟動子的轉錄通讀； gfpmut3結構基因上游還插入一段內含子序列使正反六種讀碼框的翻譯均可被終止，可保證gfpmut3以正確的讀碼框翻譯。
  20. Using the index of coincidence to identify open reading frames

    使用偶合指數方法來定出開放讀碼框架。
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