In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv
理化學研究表明,該病毒為rna病毒,對氯仿、乙醚敏感;胰酶試驗中,經37 、 1小時處理的病毒,仍然能夠在貓源細胞fcwf細胞上生長,並且毒力基本保持不變;耐酸性試驗中,病毒分別在ph5 . 0和ph3 . 0經37
作用2小時,毒力僅下降一個滴度;耐熱性試驗中,該病毒在恆定溫度50 ,設定不同時間,從5分鐘到150分鐘,毒力均有不同程度下降,其中, 50
作用30分鐘,病毒平均滴度下降2個單位; 50 , 60分鐘, cpe消失;恆定時間1小時,設定不同溫度( 50 - 60 - 70 - 80 ) ,病毒在細胞上完全喪失增殖能力, cpe消失。生物學試驗,利用實驗室現有條件,選擇不同的細胞系對該病毒進行培養,發現該病毒對貓源細胞fcwf最敏感; mdck細胞次之; f81細胞經多次傳代,亦可出現cpe ;而vero細胞則不敏感。血凝試驗表明,該病毒對
豬、雞、人及豚鼠的紅細胞均無血凝性。
The study on inhibitory effect of mienangling on cysticercus cellulosae in vitro and its toxicological roles
滅囊靈對體外
豬囊尾蚴的抑制
作用和毒理學研究
Scanning electron microscope observation of cysticercus cellulosae treated with dazolum drugs in vitro
苯駢咪唑類藥物對
豬囊尾蚴體外
作用的光鏡和掃描電鏡觀察
The scanning electron microscopic observation on cysticercus cellulosae treated with chinese materia media of compound mienangling and simple prescription in vitro
中藥復方與單方對
豬囊尾蚴體外抑制
作用的掃描電鏡觀察
Study on the enzyme - organ chemisty of the activities of saccharometabolism enzymes in the development of cysticercus cellulosae and under the drugs against cysticercus cellulosae
豬囊尾蚴發育過程中及在抗囊藥物
作用下幾種糖代謝酶的組織化學研究
From the original inputs of dietary de and dcp estimates are made step by step in simulation of the actual physiological events in the pig.
從最初輸入的飼養消化能和消化粗蛋白質測定值一步步模擬
豬中的真正生理
作用。
Expectorants are commonly used as a herd treatment and are administered in the drinking water.
祛痰劑常用
作豬群治療藥,可加到飲水中喂給。
Experiment shows that the antigen extracted by autoclave is best for dot - ppa - elisa. applying the x2 test, optimal concentration of antigen was determined, and the optimum dilution of enzyme hrp - spa was 1 : 10. the high specificity of dot - ppa - elisa was proved by the specific blocking test, and also by the cross - reaction test in which the diaphragm does not react with the antibodies against salmonellosis, pasteurellosis, chlamydiosis, hcv, ppv, brucellosis, erysipelas suis, colibacillosis, prv
試驗中對多種方法提取的診斷抗原進行了比較、篩選;確定了dot - ppa - elisa的最佳工
作條件;測定了st - 171株的免疫
豬血清抗體及c群、 2型人工感染
豬血清抗體效價,並初步確定了該方法的陽性標準。
Study on changes of collagen fibril structure in pigskin tissue after enzyme treatment
方法研究
豬皮膠原纖維在酶
作用下精細結構的變化
Promote the production of tokyo brand livestock products by aiming for shipments of 20, 000 head of tokyo x a brand of pig developed by the tmg and 30, 000 tokyo shamo game fowl - turned chicken with lean meat and so on. 2
將運送兩萬頭東京x東京都開發的
豬肉品牌和三萬頭東京軍雞由斗雞演變而來的瘦肉雞等
作為目標,促進東京品牌畜牧產品的生產。
Objective in order to understand the microbial and paracitical infection status of experimental miniature pigs and prepare spf purification program of those animals, a microbiological and parasitological study was carried out on three chinese miniature gip species : beijing wuzhishan miniature pig, guangxi barns miniature pig and guizhou xiangzhu miniature pig
摘要目的為開展實驗用小型
豬spf凈化工
作,對北京五指山小型
豬、廣西巴馬小型
豬和貴州香
豬進行微生物、寄生蟲檢測,以了解3個種群小型
豬的微生物感染狀況。
Since the important roles of eo protein in the viral infection. immunity and virus - host interaction. the homology of 21 csfv strains was investigated by sequence analysis of eo genes in this study, which will provide some evidence for epidemiological study. in addition, the eo gene of hog cholera lapinized vaccine ( hclv ) strain was expressed in the prokaryotic and eucaryotic systems, and the recombinant proteins were preliminarily analyzed by immunological method
鑒于eo蛋白在病毒感染,誘導機體免疫及與宿主細胞相互關系中的
作用,本研究克隆了2株
豬瘟病毒eo基因並將其與其它毒株eo基因進行了序列分析,揭示了我國
豬瘟病毒流行株之間的遺傳演化關系,為
豬瘟病毒的流行病學研究提供依據。
Xenotransplantation using pigs as the transplant source has the potential to resole the seere shortage of human organ donors
豬作為最具潛力的異種供器官源可以解決人類供器官嚴重短缺的問題。
The recent progress of swine as organ donors in xenotransplantation
豬作為異種器官移植供體的研究進展