At first, 1. 67 u g per well mcab all was coated on three wells of a plate, and then 1. 5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0. 1 % tween - 20 ), the bound phage was eluted with ph 2. 2 tris - gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles. ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective. after 4 rounds of panning, 11 phage clones were selected after competitive - ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged
在本實驗中,利用隨機12肽
庫對抗
豬瘟病毒( classicalswinefeverviruscsfv )糖蛋白me2的單抗a11進行表位篩選,經過四輪篩選以後,隨機挑取11個克隆作競爭- elisa檢測,結果表明,所挑11個克隆中,有9個克隆能對me2蛋白和a11反應產生抑制作用,抑制率最高可達64 ; dna測序以後經過dnastar軟體分析,發現它們的核心序列為anwralsl ,該核心序列與
豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性;夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別;人工合成含核心序列的多肽經間接elisa試驗證實,也能被a11識別。
Gray mouse tells piggy, “ you can put your fruits in the three houses
小灰鼠對小
豬說: 「這三間房子送給你做倉
庫放東西吧! 」
This proof following enterprise already through the authentication, and has set up a file in the dongguan 114 nets industry and commerce enterprise database, inquires the more detailed enterprise material, please dial the dongguan enterprise information desk 96060 ( artificially ) 9686810114 billion ( to be automatic ), this enterprise numbers for 737
茲證明東莞市大朗鎮松柏朗村大良養
豬場已通過認證,並已在東莞114網工商企業數據
庫中備案,查詢更詳細的企業資料,請撥東莞企業查詢臺96060 (人工) 9686810114 (自動) ,該企業編號為737 。
Wanda group has insisted in donating to taiyang village, jiantang township, pulandian, the counterpart in poverty relieving program, and enabled the said village to evolve into a better level from the most poverty - bitten region in pulandian by means of helping the village set up the construction team, the fruit tree base, the pig farm and the refrigerating warehouse etc
萬達集團十余年堅持捐助對口扶貧單位普蘭店市儉湯鄉太陽村,通過幫助建立工程隊、果樹基地、養
豬場、冷
庫等,使該村從普蘭店全市最貧困的地區發展到較好的水平。
In this experiment, to screen the epitope of e2 protein of classical swine fever virus ( csfv ) defined by the monoclonal antibody ( mcab ) all, which had been prepared in previous experiment, the mcab all was raised in mice and followed by purification, the concentration of protein was assayed by using the bca protein assay kit
本室利用e2基因疫苗制備了多株單抗,為e2的抗原表位研究提供了條件,我們以噬菌體隨機12肽
庫分析鑒定了
豬瘟病毒e2蛋白的抗原表位,為深入研究
豬瘟病毒的抗原結構、制備更有效的診斷試劑和疫苗提供更多理論依據。
Comprehensive utilization of animal individual mark, one - dimension code, two - dimension code and rfid electron mark, and combination of network and database with pig breeding and slaughter processing were performed to construct a tracing system suitable to chinese situation for monitoring and controlling the safe quality of pig and pork, then a system for identifying individual pig automatically was completed
摘要綜合應用動物個體標識、二維條碼、 rfid射頻電子標識和一維條碼標簽技術,將網路技術和數據
庫技術與傳統的養
豬業和屠宰加工業結合,構建了一種適合中國國情的肉用
豬和
豬肉安全質量監控的可追溯系統。
It has made the strong basis for further studying mechanism of replication, virulence and determinant, attenuation, pathogenesis, functions of genetic products, specific diagnosis, cell and host tropism, development of dna vaccine and marker vaccine of csfv, and provided an excellent tool for molecular virology. main research contents include : based on published nucleotide sequences of csfv and by the help of computer analysis software, high conservative regions and single restriction enzyme sites of genome were selected. utilizing rt - pcr and nested - pcr techniques, 7 overlapping cdna fragments covering the full genome of csfv c - strain were successfully amplified
中國
豬瘟兔化毒(脾淋毒)基因組cdna文
庫的構建、序列分析:根據已發表的
豬瘟病毒( csfv )核苷酸序列,藉助計算機軟體分析,選擇高保守區段和基因組中的單一限制性酶切位點,利用rt - pcr及nested - pcr和helf - nestedpcr技術,成功地擴增出了覆蓋c -株全基因組的7個cdna重疊片段f1 f7 ,分別克隆到pmd - 18t或pgem - teasy載體進行測序后,拼接出了其核苷酸序列。
Culex mosquitoes, which can transmit japanese encephalitis, breed in large volumes of stagnant water. they can spread the disease after biting infected pigs or wild birds
可以傳播日本腦炎的
庫蚊喜歡在大量積水中滋生,它們叮咬感染日本腦炎的
豬只或野鳥后,會傳播這個疾病。
A pair of primers containing sph i and hind iii restriction sites were designed, according to the poifn - a gene in ddbj / genbank. then poifn - a gene was cloned from porcine genomic dna by pcr
根據ddbj genbank基因
庫中已登錄的
豬干擾素基因序列,設計了含sph和hind酶切位點的一對引物,採取聚合酶鏈式反應( pcr )法,以
豬基因組dna為模板進行了poifn克隆。
Apart from conducting weekly inspection and anti - mosquito operations at the vicinity of pig farms, as well as areas where migratory birds gather and are close to human residences, the department has also launched promotion and education activities to publicise the preventive measures for culex tritaeniorhynchus
而食環署除了每周往
豬場周圍及接近民居的候鳥聚集地進行巡查和滅蚊外,還推行宣傳和教育活動,向市民宣揚防治三帶喙
庫蚊的訊息。
Building the subject database of pig breed
豬養殖專題數據庫的建設