豬瘟病毒 的英文怎麼說

中文拼音 [zhūwēnbìngdú]
豬瘟病毒 英文
classical swine fever virus
  • 豬 : 名詞[動物學] （哺乳動物） hog; pig; swine
  • 瘟 : Ⅰ名詞（中醫指人或動物的急性傳染病） acute communicable diseasesⅡ形容詞（戲曲沉悶乏味） （of tradi...
  • 病 : Ⅰ名詞1 （疾病; 失去健康的狀態） illness; sickness; disease; malum; nosema; malady; morbus; vitium...
  • 毒 : Ⅰ名詞1 （對生物體有害的性質或物質; 毒物） poison; toxin 2 （毒品） drug; narcotics 3 （姓氏） a s...
  • 豬瘟 : [獸醫學] swine fever; african swine fever; hog cholera
  • 病毒 : [醫學] virus; inframicrobe （濾過性）
  1. Hc ( hog cholera ), the virulent infectious disease caused by hcv ( hog cholera virus ), had been controlled effectively by hclv strain vaccine in the country

    豬瘟是由豬瘟病毒引起的豬的一種病毒性傳染病。豬瘟兔化弱毒疫苗有效地控制了豬瘟在我國的急性發生和大流行。
  2. Since the important roles of eo protein in the viral infection. immunity and virus - host interaction. the homology of 21 csfv strains was investigated by sequence analysis of eo genes in this study, which will provide some evidence for epidemiological study. in addition, the eo gene of hog cholera lapinized vaccine ( hclv ) strain was expressed in the prokaryotic and eucaryotic systems, and the recombinant proteins were preliminarily analyzed by immunological method

    鑒于eo蛋白在病毒感染，誘導機體免疫及與宿主細胞相互關系中的作用，本研究克隆了2株豬瘟病毒eo基因並將其與其它毒株eo基因進行了序列分析，揭示了我國豬瘟病毒流行株之間的遺傳演化關系，為豬瘟病毒的流行病學研究提供依據。
  3. The raccoon was shown by schwarte(1959)to be capable of carrying latent hcv.

    斯瓦爾特（1959）曾揭示浣熊能夠攜帶潛伏的豬瘟病毒。
  4. The rpoifn - ct antiviral activity was assayed by inhibition of the cytopathic effect ( cpe ) of vesicular stomatitis virus ( vsv ) on mardin darby bovine kidney cells ( mdbk ) or other cell lines

    並進一步在pk - 15細胞上進行抗豬瘟病毒（ hcv ）石門系（ sm ）強毒的試驗。
  5. At first, 1. 67 u g per well mcab all was coated on three wells of a plate, and then 1. 5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0. 1 % tween - 20 ), the bound phage was eluted with ph 2. 2 tris - gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles. ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective. after 4 rounds of panning, 11 phage clones were selected after competitive - ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged

    在本實驗中，利用隨機12肽庫對抗豬瘟病毒（ classicalswinefeverviruscsfv ）糖蛋白me2的單抗a11進行表位篩選，經過四輪篩選以後，隨機挑取11個克隆作競爭- elisa檢測，結果表明，所挑11個克隆中，有9個克隆能對me2蛋白和a11反應產生抑制作用，抑制率最高可達64 ； dna測序以後經過dnastar軟體分析，發現它們的核心序列為anwralsl ，該核心序列與豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別；人工合成含核心序列的多肽經間接elisa試驗證實，也能被a11識別。
  6. The glycoprotein eo of classical swine fever virus ( csfv ), besides being an envelope protein, possesses knase activity, which is pertinent to viral persistent infection in the host

    豬瘟病毒（ classicalswinefevervirus ， csfv ） eo糖蛋白既是包膜蛋白，又是一種核酸酶，其活性對病毒在宿主體內的持續感染有直接關系。
  7. The raccoon was shown by schwarte ( 1959 ) to be capable of carrying latent hcv

    斯瓦爾特（ 1959 ）曾揭示浣熊能夠攜帶潛伏的豬瘟病毒。
  8. Gx epidemic strains perhaps have a close relation to the csfv occurred in germany and italy

    但可以推測廣西近期流行豬瘟病毒與德國和義大利發生的豬瘟有密切的關系，有必要進行深入的調查研究。
  9. In order to improve the expression level of e2 gene in p. pastoris, 24 low - usage codons for p. pastoris were substitued by directed mutagnesis based on pcr

    這表明對e2基因上低利用密碼子的改造是成功的，豬瘟病毒ez基因遺傳變異及體外表達研究為進一步大規模生產打下了基礎。
  10. In order to develop recombinant poifn - a preparations preventing porcine viral infectious disease, and to further expound the research of cytokines in the field of molecular biology, mature poifn - a was cloned and expressed in e. coli expression system in the study

    為了在我國研製生物工程重組豬干擾素類制劑，防制豬病毒性傳染病和進一步開展豬干擾素分子生物學研究，本文進行了豬干擾素基因（ poifn ）的克隆、表達及其重組蛋白抗豬瘟病毒的研究。
  11. It revealed a negative reaction with positive sera of prv hcv, prrsv, jev and sa215. it revealed a positive reaction with prv standand positive serum. the results showed that the established ge - elisa has the advantages shch as high sensibility strong specificity and good repeatability and can be used to differentiation of prv infection from vaccination

    與豬細小病毒、豬瘟病毒、豬乙型腦炎病毒、 prrsv的標準陽性血清呈陰性反應，與偽狂犬病病毒標準陽性血清呈陽性反應，與三基因缺失疫苗株sa215免疫豬所采血清呈陰性反應。
  12. In this experiment, to screen the epitope of e2 protein of classical swine fever virus ( csfv ) defined by the monoclonal antibody ( mcab ) all, which had been prepared in previous experiment, the mcab all was raised in mice and followed by purification, the concentration of protein was assayed by using the bca protein assay kit

    本室利用e2基因疫苗制備了多株單抗，為e2的抗原表位研究提供了條件，我們以噬菌體隨機12肽庫分析鑒定了豬瘟病毒e2蛋白的抗原表位，為深入研究豬瘟病毒的抗原結構、制備更有效的診斷試劑和疫苗提供更多理論依據。
  13. The e2 genes above of the prevalent strain ( guangxi yulin strain ) were cloned respectively into secreted expression vector ppic9k of eukaryotic expression system p. pastoris and transformed into p. pastoris by electroporation after linearization, 25 high - copied transformants were obtained by g418 screening. it was proved that the e2 genes were integrated stably into chromosome of p. pastoris by dot blot and dna sequencing

    豬瘟病毒e2基因的真核表達：分別將csfv兩個代表株的e2基因克隆入畢赤酵母（ p . pastoris ）分泌型表達載體ppic9k中，酶切線型化后電穿孔導入p . pastotis進行整合，經g418篩選得到25個高拷貝轉化子，經dna斑點試驗和dna測序證明外源基因e2穩定地整合到p . pastoris染色體中。
  14. The expression conditions of e2 gene in p. pastoris were optimized, the results indicated that the peak obtained after 72 hours ; pattern of inhibition / induction could improve expression level ; the best ph value were between 7. 5 and 8. 0 and the optimized methanol - induced concentration was 2 % - 3 % the e2 genes of the prevalent strain ( guangxi yulin strain ) and c strain derived from rabbit spleen tissue were amplified and cloned into e. coli the expression vector pproex - htb respectively, the recombinant plasmids pproex - gxyl and pproex - c were obtained and then were transformed into the dh5a e. coli competent bacteria respectively, the recombinant bacteria could express the major antigen region of e2 gene, the expression yields amount to 35 % and 38 % repectively

    豬瘟病毒ez基因的原核表達： pcr擴增出當前豬瘟流行野毒株，中國豬瘟兔化弱毒（ c株）兔脾組織毒ez基因的主要抗原區，將其克隆到原核大腸桿菌表達載體pproex htb中誘導表達，經sds page檢測表明，重組質粒能表達ez基因主要區蛋白， westernblot檢測表明，誘導表達蛋白與豬瘟陽性血清發生特異性反應，表達量為35和38 ，可用於基因工程診斷抗原。
  15. The study is mainly about finding the reason that caused atypical hc through analysis and homology comparison on e2 gene sequences and amino acid sequences of e2 glycoprotein among strains hclv, fc, hcv - jn, hcv - yc, shimen et. al. at last, the best program against hc was established in order to control and erase hc

    本研究主要是對豬瘟兔化弱毒疫苗、豬瘟病毒野毒株抗原基因（ e2 ）進行序列分析和同源性比較，分析發生非典型性豬瘟的原因，並在此基礎上制定最佳豬瘟免疫程序。
  16. It was estimated that the yield of the e2 fusion protein in culture supernatant of recombinant p. pastoris could be reached 0. 34g / l. the optimal expression conditions were explored for the ph, aeration rate, final concentration of methanol and kinds of growth media. we conclude that the higher quantity of e2 protein can be gained at ph4. 0 - 6. 0, 1 % methanol as the final concentration, mm growth media and high aeration rate

    通過對表達條件如ph值、通氣量、誘導甲醇終濃度、培養基的選擇等條件的探索，可確定豬瘟病毒hl - ly株e2基因表達的理想條件為：最適ph值在4 . 0 6 . 0之間，最適培養基為mm培養基，最佳誘導的甲醇終濃度為1 ，加大通氣量時表達量較好。
  17. It has made the strong basis for further studying mechanism of replication, virulence and determinant, attenuation, pathogenesis, functions of genetic products, specific diagnosis, cell and host tropism, development of dna vaccine and marker vaccine of csfv, and provided an excellent tool for molecular virology. main research contents include : based on published nucleotide sequences of csfv and by the help of computer analysis software, high conservative regions and single restriction enzyme sites of genome were selected. utilizing rt - pcr and nested - pcr techniques, 7 overlapping cdna fragments covering the full genome of csfv c - strain were successfully amplified

    中國豬瘟兔化毒（脾淋毒）基因組cdna文庫的構建、序列分析：根據已發表的豬瘟病毒（ csfv ）核苷酸序列，藉助計算機軟體分析，選擇高保守區段和基因組中的單一限制性酶切位點，利用rt - pcr及nested - pcr和helf - nestedpcr技術，成功地擴增出了覆蓋c -株全基因組的7個cdna重疊片段f1 f7 ，分別克隆到pmd - 18t或pgem - teasy載體進行測序后，拼接出了其核苷酸序列。
  18. Classical swine fever ( csf ) is one of major contagious diseases in pig breeding industry. in order to gain massive diagnostic antigen csfv, and large scale of csfv specific major protective antigen protein ( e2 envelope glycoprotein ) in eucaryotic cells, which are prepared into subunit vaccine for effective prevention against csf

    豬瘟是危害養豬業的主要傳染病，為了得到大量的豬瘟病毒特異性診斷抗原，並試圖在真核細胞中大量表達豬瘟病毒特有的免疫保護性抗原，即e2囊膜糖蛋白，為進一步制備豬瘟亞單位疫苗有效預防本病奠定基礎。
  19. The signal peptide sequence which was used for secretedly expressing divergent gene in mammary gland cells was ligated to the e2 gene, the e2 gene with signal sequence was obtained by pcr. the gene resisting kanamycin was cut down from pgfp - cl vector and inserted into p22 vector, a p22 vector with gene resisting kanamycin was constructed, it was tried to construct an expression vector for transforming go

    豬瘟病毒ez基因的乳腺特異表達載體構建：將在乳腺細胞中特異分泌的信號肽序列連接到ez基因， pcr得到了目的片段，再將pgfpci載體上的kana基因切下與在乳腺細胞中特異表達的p22載體連接，使其帶有篩選標記，然後將帶有信號肽的ez插入到p22中，試圖構建山羊乳腺上皮細胞的特異性表達載體。
  20. In this study, the whole e2 genes of two strains of classical swine fever virus ( csfv ) isolated from guangxi ( gx ) were amplified by reverse transcriptase polymerse chain reaction ( rt - pcr ) method and seqenced. the e2 gene fragments of csfv were 1090 base pair in length and encoded 364 amino acid residues

    研究採用反轉錄?聚合酶鏈式反應（ rt ? pcr ）技術對兩株分別從柳州（ gxlz ）和南寧（ gxnn ）分離的廣西流行豬瘟病毒（ classicalswinefevervirus ， csfv ）進行e _ 2全基因的擴增、克隆和測序。擴增片段長度為1090bp ，編碼364個氨基酸殘基。
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