貼壁培養 的英文怎麼說

中文拼音 [tiēbìpéiyǎng]
貼壁培養 英文
adherent culture
  • 貼 : Ⅰ動詞1 （粘貼） stick; paste; glue 2 （緊挨） nestle up to; snuggle up to 3 （貼補） subsidize; h...
  • 壁 : 名詞1 （墻） wall （of a house etc ） : 銅墻鐵壁 bastion of iron2 （作用像圍墻的部分） wall of st...
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  1. These cells were polygon and displayed morphologic characteristics of liver parenchymal cells at 24 hr of culture, such as a large round nucleus with a few nucleoli and many cytoplasmic granules, sometimes binucleate, hepatocyte was in direct contact with adjacent cells

    而肝細胞培養液組在低倍鏡下觀察，培養瓶的背景干猙，死細胞較少，貼壁細胞伸出多個偽足，呈多邊形。相鄰細胞連接成小片狀。
  2. We examined the cells began to adhere 12 hr after the cells inoculated. the pseudopod were determined at 48 hr of culture. these cells adhered displayed typical epithelial cells morphological characteristics : the hepatocytes had a rich cytoplasm and were sometimes binucleate

    用含10新生牛血清的rpmi1640培養液進行培養， 12h后細胞開始貼壁生長， 48h伸出偽足，呈現典型的上皮樣細胞的外形形態，胞漿內有空泡和脂滴，可以見到雙核細胞。
  3. The primary results showed : using m199 as diluents containing 20 % bovine serum, it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example, from 0 to - 6 freezing speed is about - 0. 05 a minute, from - 6 to - 40, freezing speed is about - 0. 5 a minute ), studies on effect of various concentration of dmso demonstrate that about 12. 5 % dmso gave the highest post - thaw percentage of viable cells. the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus. the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula. the cells preserved in liquid nitrogen at - 196 were thawed and cultivated, a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 %. the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells

    研究初步表明:以細胞培養液m199 （含2既的小牛血清，常規量雙抗）為凍存稀釋液對泥鰍胚胎細胞冷凍保存宜採取先慢后快的方式（例如，從0一一6 ，凍存速度為一0 . 05 / min ，再以一0 . 5 / min的速度從一6一一40 ） ; dmso的保護效應濃度為12 . 506左右;小牛血清的濃度對泥鰍胚胎細胞的成活率影響不明顯;囊胚晚期細胞抗凍性比中早期強;通過對不同批次的凍存細胞解凍培養，解凍后成活率為30 %以上細胞培養數天後均有少數細胞貼壁，但只發現兩瓶培養細胞有明顯增殖現象產生許多未分化的小細胞。
  4. For the cryogenic preservation of fish, in this paper we made the primary culture of the kidney of allotetroploid crucian carp and primary studies were carried out on cryopreservation culture offish embryo cells derived from misgurnus auguillicaudatus or grass carp, the results of the experiments are as follows : the primary cell culture of the kidney tissue derived from allotetroploid crucian carp was carried out using tissue adherent culture, the primary observations of the growth conditions and morphology of the primary culture and subculture cells which originally come from the kidney tissue were also made

    本文主要從魚類種質保存的目的出發，一方面以四倍體鯽鯉魚為材料，對四倍體鯽鯉魚腎臟組織進行初步培養，為建立相應細胞庫及下一步培養凍存的魚類胚胎細胞奠定基礎；另一方面，以魚類組織細胞培養技術為基礎，泥鰍胚胎細胞為材料，對魚類胚胎細胞凍存培養方法進行初步研究，並應用該技術方法對草魚早期胚胎細胞進行凍存培養實驗。報告如下：本文用組織貼壁法對四倍體鯽鯉魚腎組織進行原代、傳代培養。
  5. Prolonged the culture term, hepatocytes both of experimental groups and control groups became pyknotic and were detached from the wall or fibroblasts became prominent in the culture pl ate. it was important to note that none of the condition caused an increase in the number of cell in the whole experimental process. conclusion : the primary hepatocytes in medium of additional special nutritive may benefit in comparison with common medium

    結論：在培養液中添加轉鐵蛋白、牛胰島素、煙酚胺、 p琉基乙醇以及促肝細胞生長因子，可以促進原代小鼠肝細胞的貼壁生長，並且可以改善肝細胞在體外的代謝，使之維持良好的生活狀態並且延長體外生存時間。
  6. Electron microscopy and immunocytochemical method were used to evaluate the cultivated cells

    結果：培養的周細胞貼壁生長，胞體肥大，呈不規則狀、有較多突起。
  7. Cell morphology was similar no matter what method was used. percentage of live cells was significantly higher in erythrocyte splitting method that was ( 91 4 ) % than in centrifugation method that was ( 83 5 ) %. proliferative ability of mscs seperated by erythrocyte splitting method at 2 to 6 generation was much higher than by another method. the maximal cell number was 4. 67 vs 4. 10 times that of the initial cell number at cell inoculation

    紅細胞裂解法較密度梯度離心法細胞貼壁較慢， 36小時才可見零星細胞貼壁，兩種方法細胞形態相似。活細胞百分比，紅細胞裂解法為（ gi士4 ） ，密度梯度離心治（ 83士5 ） ，有明顯差異。傳代培養： 2 6代范圍內，紅細胞裂解法較梯度離心法細胞增殖更旺盛（分別是按種時細胞數的4
  8. 3 ) dispased cold digested single cell is the best to obtain pured epithelial cell line ; it is appropriate to obtain pured fibroblast cell line that repeated attachment and the pured method to their different sensibility to trypsin

    對于秦川牛皮膚上皮細胞與成纖維細胞的純化： dispase冷消化單細胞培養的效果最佳，可獲得純化的上皮細胞系，而根據二者對胰蛋白酶的敏感性不同的分離方法、反復貼壁法適宜於獲得純化的成纖維細胞系。
  9. The cultivation on microcarriers ( mcs ) is a perfect method on the large - scale animal cell culture, which is the complete combination of adhesive culture and suspending culture

    微載體培養法是一種將貼壁培養和懸浮培養完全融為一體的較為理想的動物細胞大規模培養方法。
  10. Methods : gradient centrifugation and differential adherence were employed to isolate type pneumocytes from the lungs in the rats, proliferative activities were compared in the cultued cells from the young, adult and aged rats

    方法：應用梯度離心和貼壁培養分離大鼠肺泡型細胞，比較幼年、中年和老年大鼠培養肺泡型細胞的增殖能力。
  11. Via suspension culture and 0. 5 mol l ra induction, we established an in vitro system to differentiate es cells directly into neuron - like cells. in this system, neuron - like cells first appeared within 48h after the embryoid bodies were plated, and were most abundant 4 - 6 days after plating, while decreased 8 - 10 days later. neuron - like cells differentiated from es cells had typical morphologic properties, and specifically reacted with nf antibodies

    通過懸浮培養和0 . 5mol l ra誘導，建立了定向誘導es細胞向神經元樣細胞分化的體系。在該體系中，神經元樣細胞于類胚貼壁后48h之內出現， 46d時達到最多， 810d后減少。由es細胞分化而來的神經元樣細胞具有典型的形態特徵，並且可以與nf抗體發生特異反應。
  12. The number of adherent cells in special medium was more than that in common medium, but the morphologic difference between two groups was not significant at that time. the difference became significant after 24 hr of culture

    肝細胞培養液組貼壁的肝細胞數目明顯多於普通培養液組，但此時兩組培養液中的小鼠肝細胞在形態學上的差異並不顯著。
  13. Methods : isolated rabbit bmsc by adhesion method and cultured it at 37c, 5 % co2 incubator. graphic the growth curve after observed the growth of primary cells and passage cells. cultured bmsc in calcified solution and observe its potential of osteogenic

    方法：貼壁法分離第四軍醫大學碩士學位論文兔骨髓基質幹細胞， 37 ， 5 % co :培養箱培養，觀察原代及傳代細胞的生長發育情況，並繪制生長曲線;在礦化液中連續培養，觀察其骨形成能力。
  14. Effects of mouse embryonic fibroblast feeder layers and combined of mouse embryonic fibroblast and collagen of rat tail on adhesion of cultured cells from schistosoma japonicum

    鼠胚胎成纖維細胞飼養層及與鼠尾膠聯合促日本血吸蟲培養細胞貼壁作用的研究
  15. All study aim is to lay a foundation for clinic appliance. methods : 1 ) hpfl was isolated by enzymatic digestion derived from rat fetal liver on ed13. 5d. furthermore, erythrocyte and other cell were removed from hpfl by erythrocyte - cracking solution and different attachment method

    研究方法： 1 ）取ed13 . 5d的大鼠胎肝，酶消化法離散細胞，用紅細胞裂解液去除紅細胞，差速貼壁法去除其它細胞，接種于不同的基質和培養液中， mtt法比較不同培養液和培養基質對大鼠hpfl的體外生長影響。
  16. A successful cell strain for biopharmaceutical production must conform to the following characteristics : the cells could produce high level of recombinant proteins ; the cell should be adapted to serum - free or protein - free medium ; the cells should be resistant to adverse culture conditions, which means the cells should have some anti - apoptosis property ; if not cultured i n suspension, the cells should also be able to grow in adherence

    一株成功的工程細胞除了要求目的蛋白的表達量高外，還必須適應無血清培養基培養；必須具有對不利環境的抵抗能力，即抗凋亡能力；對于非直接懸浮培養的細胞，還必須具備在無血清培養條件下的貼壁能力。
分享友人
分享到 Line

分享到臉書