質粒免疫 的英文怎麼說
中文拼音 [zhílìmiǎnyì]
質粒免疫
英文
immunity in plasmids-
6. oocytes were fixed for immunofluorescence. examination of cgs and microtubules were performed by fitc labeled lens culinaris agglutinin ( lca ) and and - fi - tubulin under confocal scanning laser microscopy ( cslm ) respectively
利用直接免疫熒光染色和共聚焦顯微鏡（ confocalscan muglasermicroscopy ， cslm ） ，研究各組體夕成熟卵的皮質顆粒和微管The distribution of the brine shrimp hgcs varies greatly from the species studied till now. one hour after hatching, neither the dorsal - anterior area nor the other dorsal area remained positive immunoreactivity signal. and 2 hours after hatching, there was no typical hgcs in the body of the brine shrimp and the remained hatching enzymes may participate in digesting the left vitellin in the nauplius
鹵蟲hgc最初出現至孵化前1h時均為全身性分佈，從孵出到孵出后2h ，頭鹵蟲孵化酶的生物化學性質及孵化腺細胞的免疫組織化學研究部的孵化酶顆粒已經減少，而變為非全身性分佈，到孵出后sh ，孵化酶顆粒已基本消失殆盡。Protective immunity to schistosoma japonicum elicited by co - immunization of cathepsin b dna vaccine with eukaryotic plasmid encoding il
4真核表達質粒聯合免疫誘導小鼠保護性的研究Ip3 - ip3 receptor ( ip3r ) interaction mediates the release of ca2 + from the endoplasmic reticulum in response to many different extracellular stimulus. for higher plants, however, though it is now generally accepted that ip3 participates in signal transduction in many important cellular processes, only limited evidence is available for the presence and properties of the ip3r - like protein so far. here, using the immunological methods with an antibody raised against a mammalian inositol 1, 4, 5 - triphophate receptor ( type 1 ), we found that, 1 ) the antibody across - reacted the proteins with about 200kd in microsomes from oryza sativa and about 200kd from arabidopsis thaliana respectively
本實驗用sds - page電泳和免疫印跡的方法，用哺乳動物大鼠三磷酸肌醇受體的多肽做抗體對類三磷酸肌醇受體蛋白鑒定，結果表明：抗體與水稻和擬南芥微粒體蛋白分子量大約為200kd的蛋白交叉反應，同時還發現在水稻微粒體蛋白62kd和擬南芥微粒體蛋白45kd處有交叉反應的蛋白條帶存在，表明在植物中有類三磷酸肌醇受體蛋白的存在；用免疫膠體金方法，發現類三磷酸肌醇受體蛋白主要分佈於液泡膜和細胞質膜上。By sds - page and immuno - blotting, we found that a monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain antibody could react with cytoplasmic dynein intermediate chain - like protein at 67 kda in lily pollen. under confocal laser scanning microscopy after immunoflurescence labeling, we found that the dynein intermediate chain - like protein appeared punctated and was co - localization partly with microtubules in cytoplasm of lily pollen tube
免疫熒光標記及激光共聚焦掃描顯微鏡觀察發現，類細胞質力蛋白中間鏈在百合花粉管中存在於顆粒狀細胞器上；免疫熒光雙標及激光共聚焦掃描顯微鏡觀察發現，百合花粉管中類細胞質力蛋白中間鏈和微管存在部分共分佈。2, the electron dense area ( eda ) consisting of the astrocytic process on one side and the neuron ( dendrite ) on the other side was observed in immune - electron - microscopic staining studies, and the eda was characterized with double layers thickening and dark staining cytomembranes with a narrow cleft between them
（ 2 ）免疫電鏡觀察， son內星形膠質細胞與神經元接觸部位可以觀察到膜增厚的結構? ?電子緻密區（ edas ） ，在神經元一側可見cx32陽性金顆粒，而在星形膠質細胞一側可見cx43陽性物質分佈。After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company
實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。Goat anti - human ige antibody were used as second antibody to make sure that the positive clones were ige related. through three cycles of screening, the inserted cdna fragments of the positive clones were amplified by pcr and sequenced. the results showed that the inserted cdna fragment from one clone was 1200 bp in length, with a orf of 507 bp which encoded 169 amino acids
Sj43b pgex 6p 1重組質粒的誘導表達、表達產物的鄉寸和免疫學性質鑒定分析為獲得可溶性的rsj43b月6gsta蟲合蛋白，對不同iptg誘導劑濃度、誘導表達溫度和誘導表達時間等因素對融合蛋白可溶性表達的影響進行了觀察。Salmonella typhimuriwn, one of the invasive bacterial species, can be attenuated without loss of invasiveness and thus used for delivery of eukaryotic expression vectors into host cells in vivo. the recombinant plasmid containing the target gene is released inside the host cells and gain entry into the nucleus, resulting in expression of encoded antigens and subsequent induction of humoral and cellular immune responses
沙門氏菌（ salmonellatyphimurium ）是一種較為常見的侵襲性胞內菌，通過基因工程方法減毒后對宿主致病性顯著降低，但仍保留良好的侵襲力，可直接將真核表達質粒攜帶進入動物細胞內表達相應的蛋白而誘導特異性的免疫應答反應。Much difference of the t cell subpopulations was observed among individuals after immunization. the percentages of cd4 +, cds + or cd4 + / cd8 + t cells of the animals immunized with dna vaccines increased at different extents compared to the pires1neo - immunized or mock - immunized animals
與空白對照組和空質粒p舊esineo免疫組比較，各重組質粒免疫組動物的cd4 、 c08及cd4 ic08 t細胞有不同程度的升高。It can be concluded that the elevated ability of penaeus immune system is realized via increasing po production instead of increasing of unit enzyme activity of po. since the biggest number of hemocytes, semigranular cells and po production have been obtained after - 1, 3 - glucan and lps stimulation, it implies that the - 1, 3 - glucan and lps would be the most efficient immunostimulant on shrimp, penaeus chinensis, among the immunostimulants used
透射電鏡下的觀察結果表明，中國對蝦血細胞的超微結構在免疫刺激前後也發生了變化，三種血細胞的糙面內質網、核糖體和線粒體的數量均有一定程度的增加，其中以小顆粒細胞和大顆粒細胞的超微結構變化幅度最大。These suggested that so2 may affect body immunity to a certain degree. ( 3 ) effects on mouse thymus of so2 challenge : he staining showed no obvious structure changes of thymus in all treatment groups compaired with the control group ; the ultrastructure of thymus can be seen injured in so2 treated groups from tem observation
（ 3 ）二氧化硫染毒對小鼠胸腺組織學結構影響較小：用透射電鏡觀察發現胸腺組織中有部分淋巴細胞變形且異染色質增多，胸腺上皮細胞中可見次級溶酶體增多，線粒體變形，這說明二氧化硫可能對中樞免疫器官也有一定的不良影響。Objectives to improve the effect of a single mtb8. 4 dna vaccine, we constructed a chimeric mtb8. 4 / hil - 12 eukaryotic plasmid by linkage of mycobacterium tuberculosis mtb8. 4 gene to human il12 gene with a simple linker ( gly4 - ser ) 3. we analyzed the immunogenicity of chimeric dna vaccine and investigated the immune responses elicited when mtb8. 4 / hil12 was presented as endogenous ag
目的：以il - 12作為分子佐劑，與結核桿菌新抗原mtb8 . 4基因連接形成嵌合分子，將其克隆到真核表達質粒中，構建成嵌合dna疫苗，研究其在小鼠體內誘導細胞免疫應答的效果及對c57bl 6n小鼠的免疫保護作用，為尋求安全、有效、廉價的結核病新疫苗打下基礎。As well as in eukaryocyte ( hepg2 and cos - 7 ), then detect their antigenity as a basis study and explore of the choice of immunogen for preventive and therapeutic vaccines of hepatitis b. methods : the gene fragments coding 152aa ( si ) and 124aa ( s2 ) of the carboxyl terminus of hbsag were amplified by pcr from plasmid pecob6 with a pair of primers containing different endonuclease sites and were cloned into multiple cloning sites of plasmid pbks ( + )
為乙型肝炎的預防和治療性疫苗免疫原的選擇進行初步的研究和探討。方法：本研究利用聚合酶鏈反應（ pcr ） ，通過設計帶有不同酶切位點的一對引物，從質粒pecob6特異性擴增hbsag蛋白羧基末端152個氨基酸（ s1 ）和124個氨基酸（ s2 ）的基因片段，分別將二者克隆到質粒pbks （ + ）的多克隆位點，篩選重組克隆。Global or filament actin was visualized in cytoplasm and nucleus of tobacco pollen mother cells, under immunoelectron microscope
免疫電鏡觀察發現，花粉母細胞中的細胞質及核中存在線狀和粒狀的肌動蛋白微絲。Haake da, champion ci, martinich c, et al. molecular cloning and sequence analysis of the gene encoding ompl1, a transmembrane outer membrane protein of pathogenic leptospira spp [ j ]. j bacteriol, 1993, 175 ( 13 ) : 4225
晏菊芳，鮑朗，伍衛華，等.中國鉤端螺旋體強毒株017膜蛋白基因的質粒載體構建及表達[ j ] .中華微生物學和免疫學雜志， 1999 ， 99 （ 2 ） : 117Furthermore, we discussed the influence of immune - sensibilization before induction to the activity of t - cell vaccine, compared the immune - sensibilization difference between hcv - adenovirus vectors and plasmid vectors
此外，我們還探討了誘導前免疫致敏對t細胞疫苗活性的影響，並比較了hcv腺病毒載體和質粒載體在此免疫致敏功能方面的差異。In this study, by combining rt - pcr and race, a full - length cdna encoding the lagurus lagurus zp3 ( lzp3 ) was isolated from the lagurus ' s ovary and cloned into a prokaryotic expression vector and a eukaryotic expression vector for further immunocontraceptive investigations
本論文研究工作採用rt - pcr與race相結合的方法，首次克隆了草原兔尾鼠zp3全長cdna並進行了序列分析，構建了真核表達質粒，為草原兔尾鼠免疫不育的研究奠定了基礎。Expressions of fas fasl in colorectal carcinoma and adjacent mucosal tissues
1基因真核表達重組質粒的構建及免疫效果分享友人