質粒復制 的英文怎麼說

中文拼音 [zhílìfùzhì]
質粒復制 英文
plasmid replication
  • 質 : Ⅰ名詞1 （性質; 本質） nature; character; essence 2 （質量） quality 3 （物質） matter; substance;...
  • 粒 : Ⅰ名 （小圓珠形或小碎塊形物） small particles; grain; granule; pellet Ⅱ量詞（用於粒狀物）
  • 復 : Ⅰ形容詞1 （重復） repeated; double; duplicate 2 （繁復） complex; compound Ⅱ動詞1 （轉過去或轉過...
  • 制 : Ⅰ動詞1 （製造） make; manufacture 2 （擬訂; 規定） draw up; establish 3 （用強力約束; 限定; 管束...
  1. Features : contain lemon grass, jojoba essential oil and rich mineral deep sea salt particles, which can clean out the aged cutin on the skin surface, promote the blood circulation and metabolism, destroy and resist bug, dispel inflammation. its fresh lemon grass fragrances can dispel the beriberi and foot bad smells, clear out the bad smells, relief the tired foot, get rid out of the swollen feeling, increase the funny interests by washing foot, let the foot to recover fresh and energetic completely

    特點：蘊含的檸檬草、荷荷巴等香薰精油及含豐富礦物質的深海鹽幼細粒子，溫和清除積聚于肌膚表面的老化角質，促進血液循環及新陳代謝，殺滅並抑制病菌，消除足部炎癥，清新自然的檸檬草芬芳，有效祛除腳氣、腳臭，清除腳部異味，同時舒緩疲倦雙足，消除腫脹的感覺，增添浴足情趣，讓浸泡后的雙足徹底恢復清爽與活力！
  2. Glucose biosensors were then constructed by these nanocomposites, and their electrochemical properties had been explored. secondly, the self - assembled nanocomposite was formed by mwnts and biopolymer, and was used to study the electrochemical properties of nadh. and finally, the modified electrode which was formed by immobilizing small molecular onto electrode surface, was used to detect dsdna in the solution

    本論文首先，將多壁碳納米管（ mwnts ）與納米顆粒相結合，制備的納米復合材料用於構建葡萄糖生物傳感器，並研究了它的電化學性質;然後，將mwnts與生物聚合物自組裝制備的納米復合材料用於研究nadh的電化學性質;最後，本文還將小分子自組裝固定在電極表面，用於測定溶液中的dsdna 。
  3. A small cryptical plasmid pefr was isolated from enterococcus faecium strain df101. the complete sequence analysis of the plasmid show that it consists of 3176 bps, which contains four putative orfs. orf1 encodes a putative protein and is highly similar to repa which functions in replication

    從屎腸球菌df101菌株中分離到隱秘的小質粒pefr ，全序列分析顯示質粒pefr由3176bp組成，編碼四個推定的orf ， orf1編碼的一個推定的蛋白和復制有關的repa有很高的相似性。
  4. Firstly, the surface characteristic of polystyrene particle is changed from water detesting to water intimity by using special techniques and admixtures so mat the compound quality with inorganic materials is insured. secondly, the contradiction between the weight and strength is solved through optimizing the particle size and using composite fiber and the best heat conductivity is achieved under the condition that the necessary strength is met. in the research process, the author solved the problem of fiber dispersing in insulating materials so that the contraction of the material is controlled

    課題研究中，首先採用特殊的改性工藝及外加劑實現對聚苯乙烯顆粒表面的成功改性，使其表面由憎水轉化為完全親水，確保與無機材料的復合質量；其次，通過採用優化骨料級配及使用復合纖維等措施解決了保溫材料的輕質與強度的矛盾，使保溫材料在滿足必要的強度的前提下，導熱系數降至最小；並且，課題研究中成功解決了纖維在保溫材料中均勻分散的問題，達到了抑制保溫材料收縮的目的；最後，通過採用復合外加劑、合適的膠凝材料及合理的配比等措施確保該保溫材料具有良好的和易性，滿足施工的要求。
  5. Since the plasmid is capable of independent replication in host cells of many dicotyledonous plants, it has been used as a cloning vector in gnetic engineering

    在許多雙子葉植物中質粒在寄主細胞中是獨立的復制單元，所以可以在基因工程中用作克隆載體。
  6. In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv

    本研究採用脂質體轉染方法，將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞，經6輪蝕斑克隆、篩選、表達， pcr鑒定和dot - elisa檢測，證明該重組病毒已構建成功，並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。
  7. In our laboratory, a unique mutation detection system using a shuttle vector plasmid has been established to demonstrate that a low concentration of mnng ( 0. 2 m ) can induce nontargeted mutation in mammalian cells : the mammalian cells were exposed to 0. 2m mnng for 2. 5h, then a shuttle plasmid pz189 carrying supf trna gene was transfected into cells after 24h culture. we found a 5 - fold higher mutation frequency of the plasmid replicated in pretreated cells than the spontaneous mutation frequency of the plasmid replicated in control cells. this kind of mutation did not occur immediately after mnng exposure

    我們實驗室曾用一特殊的突變檢測系統，直接證明dna損傷劑可在哺乳動物細胞誘發非定標性突變：首先用低濃度（ 0 . 2 m ）的短壽烷化劑mnng （半壽期為1 . 1hr ）處理細胞2 . 5h后，繼續培養24h ，將重組有用作突變檢測的靶基因supftrna基因的穿梭質粒pz189轉入細胞復制，發現在未受致癌物直接攻擊的穿梭質粒中有較自發突變率高5倍以上的靶基因突變。
  8. Complete sequence analysis of the two plasmids reveal that the small plasmid plp2000 with 2061 nucleotide encodes a putative 37 kda replicase. and in the downstream region of the replicase gene, it existed multi - tandem repeat of 17 bps

    Southernblot顯示plp2000和plp9000兩種質粒屬于滾環復制類型質粒，和滾環復制相關的鉆序列可能形成的二級結構分別為sso和ssou類型。
  9. Construction of a resolution vector with a small plasmid origional replion a small plasmid original replion about 2062bp come from b. thuringiensis subsp. kurstaki strain ybt - 1520 and the res site of tn4430 were used to construct a resolution vector

    利用小質粒復制區構建解離載體利用tn4430的解離區和來源於庫斯塔克亞種大小為2062bp的小質粒復制區ori2062構建解離載體pbmb1125 。
  10. Kurstaki strain hd73, were inserted into two copy sets of res sites. the res sites have same direction. when - the recombinant plasmid was introduced into crystal negative b. thuringiensis host bmb171, antibiotic resistance genes and other non - 5, thuringiensis dna can be selectively eliminated after the selection by antibiotic resistance marker

    將crylac10基因或壯觀黴素基因和蘇雲金芽胞桿菌的質粒復制起始區oril030連接在一起，置於兩個同向的解離區之間，再將基因操作中所必需的大腸桿菌質粒復制起始區和抗生素標記基因等與之相連構成解離載體。
  11. In this paper, the so preparing self - compacting concrete with low and middle strength using super fine mountain sand artifical sand and phosphorus slag is studied systemically. through the study the influence of the shape, the proportion of the dust and the grade of sand to the workability and the mechanical property of self - compacting concrete, it is found that super fine mountain sand artifical sand are not suit to prepare low and middle strength self - compacting concrete, but when they are combined and the additive and assistant band material are used, c20 - c40 high performance self - compacting concrete is prepared ; the corresponding additive is developed to solve the delamination caused by the low proportion of banding material ; the evaluating system for the workability of low and middle strength self - compacting concrete is built ; the controlling method for production, construction and curing was set up. and the result of the research is applied into several projects

    通過分析特細山砂和機制砂的顆粒形態、粉末含量、顆粒級配等特性對自密實混凝土的工作性能和力學性能的影響，發現特細山砂、機制砂不宜單獨用於配製中低強度等級自密實混凝土，宜將特細山砂和機制砂進行合理復配，並選擇合適的外加劑和礦物摻合料，優化配合比設計，可生產c20 c40中低強度等級自密實高性能混凝土；研製開發出了具有高效減水、保塑、抗離析功能的外加劑，有效解決了中低強度等級自密實混凝土由於膠凝材料用量少而出現的離析、泌水問題；應用正交設計方法，對因素和水平進行合理選擇，確定了生產中低等級自密實混凝土的最優配合比；建立了中低強度等級自密實混凝土的工作性評價體系；提出了生產、施工及養護的質量控制技術方法。
  12. A bt - e. coli shuttle vector pht315 was deleted its replication region of bt, then constructed a novel vector named pht315 - 1 which composed a multiple cloning site, erythromycin and ampicillin - resistance marker and could only replicated in e. coli. used pht315 - 1, a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324. sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501, 333, 183aas. orfl had 98 % identities with replicating related protein ori43 of bt strain hd263. the others were no homology to any published bt replicating related protein. after continuous cultured for 70h at 30 c without antibiotic selecting press. the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 %. and growth curve also showed that the novel replicon was stable and could replicate normally

    進一步序列分析表明該復制區至少有3個較大的orf ，分別編碼501 ， 333 ， 183個氨基酸。其中orf1蛋白序列與hd263復制蛋白ori43的同源性為98 ，而另外兩個orf和genbank己公布的bt復制相關蛋白無同源性。 30連續培養72h ，復制區質粒在bt無晶體突變株hd73cry ~ -中穩定性達98以上， 30h生長曲線也表明該復制區能夠在bt中穩定復制和遺傳，對受體菌株無明顯不良影響。
  13. Dnd phenotype could be detected when the entire dnd cluster was integrated in the chromosome or carried by the low copy - number plasmid in zx1. however, when dnd cluster was carried by a plasmid with a copy - number around or higher than c. 10 copies in zx1, dnd phenotype could not be observed

    當完整的dnd基因簇整合在zx1染色體上或由1 2個拷貝的自主復制的質粒攜帶進入zx1 ，都能在zx1中檢測到dnd表型，但是由10個或超過10個拷貝的質粒攜帶進入zx1 ，就檢測不到dnd表型。
  14. To create transgenic mushrooms, researchers attached a gene that confers resistance to hygromycin, an antibiotic, to circular pieces of bacterial dna called plasmids, which hae the ability to multiply within a bacterium known as agrobacterium

    為了創造轉基因蘑菇，研究人員需要把一種抵抗潮黴素（一種抗生素）的基因片段插入細菌dna的圓形片段（也叫質粒）中去，它將隨著土壤桿菌復制而大量復制。
  15. Construction of two vectors containing different plasmid original replicon this work constructed four resolution shuttle vectors, pbmb1205, pbmb1205r, pbmb1206 and pbmb1206r. there are multiple clone sites between two copies of res sites

    解離載體的構建和性能利用來源於不同質粒上的質粒復制起始區ori44和ori1030構建了4個解離載體。
  16. The structure icon is part of the molecular model of lysozyme, modified from molecular biology of the gene 1976 plate 3 ; the purification icon is from a manual of pharmacia ; the analysis icon is part of a drawing by juang

    上面作為三大主題的縮圖圖片，蛋白質構造是lysozyme的分子模型，自watson所著molecular biology of the gene 1976 plate 3所復制;中間的膠體粒子電顯照片取自pharmacia說明書;右邊蛋白質轉印圖，是自繪的幻燈片。
  17. There is typical - 10 region, - 35 region and rbs sequence on the upstream of orf1 and on the upstream of the - 35 region there are four tandem repeats of 22 bps sequence, and similar sequence was found on the upstream of the replicase genes of pmbbl, pvs40, plu510

    在orf1上游具有典型的- 10區、 - 35區和rbs堿基序列， - 35區上游附近四段22bp正向串聯重復序列，相類似的序列也見于質粒pmbb1 、 pvs40 、 plq510復制蛋白酶基因上游。
  18. The btl - btll promoter was found to drive gfp gene expression strong green fluorescence in not only b. thuringiensis but also e. coli strain. however, cry3a promoter could not drive gfp gene expression in e. coli, and the expression i

    通過亞克隆和dna缺失實驗，確定orf ]包含在與復制功能有關的2 . 2kb區段內，因此推斷ol : fj基因編碼的是該質粒的復制蛋白。
  19. A cdna subtractive library with high subtractive efficiency of repeated + gz exposures in rat brain was constructed with suppression subtractive hybridization ( ssh ). the cdna subtractive library after amplification included 100 blue clones and 400 white clones, 75 ones of which were selected to prepare for plasmid. identification of the clones with restriction endonuclease cleavage showed most of them had been cloned to the vector

    構建高消減效率的+ gz重復暴露大鼠腦cdna消減文庫，擴增后cdna文庫包含約400個白色克隆和100個藍色克隆，克隆飽滿清晰，隨機挑取75個白色克隆，制備質粒后，以ecor酶切分析，表明大部分克隆入質粒載體。
  20. The restriction map of the plasmid pbl29 was tentatively constructed according as the molecular weights of fragments of plasmid pbl29 digested with different enzymes. analysing the sequence of the plasmid pbl29 tested, we found that the mol ecular weight of the plasmid pbl29 is 371 lbp, that it s many unique restriction endonucleases, km resistance genes and abundant a / t base pairs of replicating site are on the plasmid pbl29

    同時根據限制性酶切各片段的分子量作出了質粒pbl29的內切酶圖譜。並對質粒pbl29進行測序和分析，證明了質粒pbl29大小為3711bp ，具有大量的單酶切位點、編碼卡那黴素抗性基因以及富含a t序列的復制起點。
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