質粒載體 的英文怎麼說

中文拼音 [zhílìzǎitǐ]
質粒載體 英文
plamidvector
  • 質 : Ⅰ名詞1 （性質; 本質） nature; character; essence 2 （質量） quality 3 （物質） matter; substance;...
  • 粒 : Ⅰ名 （小圓珠形或小碎塊形物） small particles; grain; granule; pellet Ⅱ量詞（用於粒狀物）
  • 載 : 載Ⅰ名詞（年） year : 一年半載 six to twelve months; six months to a year; 三年五載 three to five ...
  • 體 : 體構詞成分。
  • 載體 : [化學] carrier; supporter; isotopic carrier
  1. First, to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp, and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second, based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins, the concentration of the hab toxins could be detected

    目的：構建一個含c - fos啟動子和egfp報告基因的pegfp - c - fos重組質粒載體。體外轉染膀胱癌biu - 87細胞后，利用赤潮毒素作用后細胞表達綠色熒光蛋白的變化來檢測赤潮毒素，初步建立一種以細胞為基礎受體水平的赤潮毒素檢測方法。
  2. In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli

    根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。
  3. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  4. This paper describes a strategy that has developed to transfer the cdna of tobacco mnsod gene into the commercially important breeding line - baoding alfalfa via agrobacterium infection. transgenic alfalfa plants have been generated that overproduce a nicotiana plumbaginifolia l. manganese superoxide dismutase ( mnsod ). the results domenstrated that baoding alfalfa is an important breeding line which easily amenable to genetic transformation

    本研究採用我國農藝性狀優良的豐產苜蓿品種保定苜蓿，通過農桿菌介導的轉基因方法，使用特定的質粒載體pchlsod將煙草mnsod基因的cdna序列導入保定苜蓿中，說明保定苜蓿是一種易於遺傳轉化的優良苜蓿育種品系。
  5. The recombinant expression plasmids prt - p450nor and pet - p450nor were constructed by inserting p450nor gene into the bamh i / hind iii site of the prokaryotic expression vector prset and pet28, then they were transformed into e. coli bl21

    本研究將已克隆的真菌細胞色素p450nor基因插入原核表達質粒載體prset和pet28的bamhi / hind位點，成功構建重組表達質粒prt - p450nor和pet - p450nor ，並轉化到e . colibl21 。
  6. In order to express alkaline protease gene ( ap gene ) in bacillus subtil is, the recombinant expression plasmid was constructed. this plasmid contains a promoter bp53, also from b. pumilus un - 31 - c - 42, ap gene and the shuttle vector psugv4. after introduced into b. subtilis wb600, the transformants displayed the hydrolyzed zone on milk plate

    將來自短小芽抱桿菌un一31一c礴2的基因啟動子（ bp53片段）和脫毛蛋白酶全基因（ ap ）進行融合，然後將重組基因（命名為bpap ）插入到大腸桿菌-枯草桿菌穿梭質粒載體psugv4中，構建成表達質粒psu一bpap 。
  7. By using ssh, a cdna subtractive library of aloe under salt stress had been done

    以經過驅逐方cdna消減的第二次pcr產物與t a質粒載體連接，建成cdna消減文庫。
  8. According to above consideration, experiments was carried out as below : first, targeted gene - human serum albumin ( hsa ) gene was obtained via pcr technology. secondly, the hsa gene was liganded with a plasmidprla22 whose two arms carry dna sequences necessary for crossing with the human a - lactalbumin yac to form an integration vector prla - hsa, then the integration vector plasmid was co - transformated into the right yac - bearing yeast with another plasmid plrh33 which carrys a selective gene

    因此，本試驗首先擴增出整合在酵母基因組里的人血清白蛋白（ hsa ）基因作為目的基因，並將人血清白蛋白基因插入到一個含有人-乳白蛋白yac同源序列的重組型質粒載體，以構建整合型載體，再與另一個帶篩選基因的質粒共轉化入含人-乳白蛋白yac的酵母細胞體內。
  9. A method to construct 12 kb plasmid vector

    重組質粒載體的方法
  10. Result, we succeed in doing t - pa gene from fetal lung and construct a sort of eukaryon expression plasmid vector with pcdna3. 1 ( + )

    結果：成功地從胎兒肺組織中克隆了t - pa基因，並構建了以pcdna3 . 1 （ + ）為載體的真核表達質粒載體。
  11. In order to construct plant artificial chromosome, plasmid vectors have been constructed to integrate necessary elements into both right and left yac arms

    為了構建植物人工染色體，我們構建了可以和含有著絲粒序列的yac載體的左臂和右臂進行同源重組的質粒載體。
  12. Hydrophobicity analysis suggested that the protein was highly hydrophilic, especialy at the first 24 amino - acid, this region could be function as signal peptide

    將此基因重組到表達質粒載體pgex - 4t - 1上，轉化到大腸桿菌bl21 （ de3 ）中，經iptg誘導后，可得到高效表達。
  13. Haake da, champion ci, martinich c, et al. molecular cloning and sequence analysis of the gene encoding ompl1, a transmembrane outer membrane protein of pathogenic leptospira spp [ j ]. j bacteriol, 1993, 175 ( 13 ) : 4225

    晏菊芳，鮑朗，伍衛華，等.中國鉤端螺旋體強毒株017膜蛋白基因的質粒載體構建及表達[ j ] .中華微生物學和免疫學雜志， 1999 ， 99 （ 2 ） : 117
  14. 2. ecori / bamhi digested pcr products were inserted into the corresponding restriction site in the expression plasmid pbv220. construction of non - fusion expression plasmid pbv220 - endostatin

    用ecori和bamhi酶切endostatincdna的pcr產物，將其插入到質粒載體pbv220中相應的限制性酶切位點，構建非融合質粒表達載體pbv220 - endostatin 。
  15. Furthermore, we discussed the influence of immune - sensibilization before induction to the activity of t - cell vaccine, compared the immune - sensibilization difference between hcv - adenovirus vectors and plasmid vectors

    此外，我們還探討了誘導前免疫致敏對t細胞疫苗活性的影響，並比較了hcv腺病毒載體和質粒載體在此免疫致敏功能方面的差異。
  16. Objective, clone tissue - type plasminogen activator ( t - pa ) gene and construct a new kind of recombinant vector containing human tissue - type plasminogon activator ( t - pa ) cnda neither cytotoxiaty nor actovating prot - oncogenes

    目的：克隆組織纖溶酶原激活物（ t - pa ）基因並構建一種無細胞毒性、不激活原癌基因的真核表達的pcdna3 . 1 （ + ） / t - pa質粒載體。
  17. The recombinant plasmids pbmb121l, pbmb9821l and pbmb986l were constructed after transferring bacillus thuringiensis icps gene crylaal, crylaclo and crylca from their original plasmid vectors to different plasmid vector. the relevant recombinant strains were obtained after introducing the 3 recombinant plasmids into bacillus thuringiensis plasmid - free mutant strain 8mb 171 by electroporation. the transformation and expression properties of 8mb 171 were studied

    通過將蘇雲金芽胞桿菌殺蟲晶體蛋白基因cry1aal 、 cry1ac10和cry1ca轉移至不同質粒載體上，分別構建了重組質粒pbmb121l 、 pbmb9821l和pbmb986l ，並分別導入無質粒突變株bmb171 ，篩選得到攜帶相應icps基因的重組菌株。
  18. 4 we observed that t cell vaccine activity could be greatly improved through immune - sensibilization to mice with hcv adenovirus vectors or plasmid vector before t - cell vaccine induction, and adenovirus vectors proved to be superior to plasmid vector

    在誘導t細胞疫苗之前，用v腺炳毒載體或質粒載體討小鼠進廳免疫致敏，可以明顯提高t細胞疫苗的活性；其中腺病毒載體的免疫致敏效果要明顯好於質粒載體。
  19. A cdna subtractive library with high subtractive efficiency of repeated + gz exposures in rat brain was constructed with suppression subtractive hybridization ( ssh ). the cdna subtractive library after amplification included 100 blue clones and 400 white clones, 75 ones of which were selected to prepare for plasmid. identification of the clones with restriction endonuclease cleavage showed most of them had been cloned to the vector

    構建高消減效率的+ gz重復暴露大鼠腦cdna消減文庫，擴增后cdna文庫包含約400個白色克隆和100個藍色克隆，克隆飽滿清晰，隨機挑取75個白色克隆，制備質粒后，以ecor酶切分析，表明大部分克隆入質粒載體。
  20. In this research, the gpv hl isolate was propagated with 13 day ' s duck embryos. a pair of primers gflgr used to arnplify vp3 gene was designed using oligo4. i software according to the whole nucleotide sequence of gpv b isolate published by zadori. the major structural protein vp3 gene was amplified from the dna of gpv hl isoiate by polymerase chain reaction ( pcr ), and then cloned into pmdl8 - t vecter

    根據zadori等發表的gpvb株全基因核苷酸序列，藉助oligo4 . 1軟體設計了1對用以擴增主要結構蛋白vp3基因的引物gf / gr ，通過pcr技術，從病毒基因組dna中擴增出病毒主要結構蛋白vp3完整基因片段，經酶切鑒定后直接與pmd18 - t質粒載體連接。
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