載體蛋白質 的英文怎麼說

中文拼音 [zǎitǐdànbáizhí]
載體蛋白質 英文
carrier protein
  • 載 : 載Ⅰ名詞（年） year : 一年半載 six to twelve months; six months to a year; 三年五載 three to five ...
  • 體 : 體構詞成分。
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 質 : Ⅰ名詞1 （性質; 本質） nature; character; essence 2 （質量） quality 3 （物質） matter; substance;...
  • 載體 : [化學] carrier; supporter; isotopic carrier
  • 蛋白質 : [生物化學] protein; proteide （舊稱「朊」, 由多種氨基酸結合而成的高分子化合物）
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. First, to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp, and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second, based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins, the concentration of the hab toxins could be detected

    目的：構建一個含c - fos啟動子和egfp報告基因的pegfp - c - fos重組質粒載體。體外轉染膀胱癌biu - 87細胞后，利用赤潮毒素作用后細胞表達綠色熒光蛋白的變化來檢測赤潮毒素，初步建立一種以細胞為基礎受體水平的赤潮毒素檢測方法。
  2. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  3. Effects of silicon surface morphology on protein chelating

    矽片載體表面形貌對蛋白質吸附的影響
  4. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  5. In order to express alkaline protease gene ( ap gene ) in bacillus subtil is, the recombinant expression plasmid was constructed. this plasmid contains a promoter bp53, also from b. pumilus un - 31 - c - 42, ap gene and the shuttle vector psugv4. after introduced into b. subtilis wb600, the transformants displayed the hydrolyzed zone on milk plate

    將來自短小芽抱桿菌un一31一c礴2的基因啟動子（ bp53片段）和脫毛蛋白酶全基因（ ap ）進行融合，然後將重組基因（命名為bpap ）插入到大腸桿菌-枯草桿菌穿梭質粒載體psugv4中，構建成表達質粒psu一bpap 。
  6. Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing

    目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。
  7. Many substances in our body need to be transited from here to there by blood, and some other substances need some proteins functioned as carriers in the plasma lemma to enter cells

    體內不少物質需要通過血液從一處運送到另一處，也有一些物質需要質膜中的某些蛋白質作為載體而進入細胞。
  8. The results demonstrated that the momps were protective antigens and the momp - iscoms of aeromonas hydrophila could induce the host to mount satisfied immunity. a pair of primers were designed according to the published nucleotide sequence of a putative outer membrane protein gene ( omp ) of aeromonas hydrophila. with the specific primers, a target fragment about 1. 1kb was amplified from aeromonas hydrophila l316 via pcr. the target fragment was inserted into the linearized pgem - t easy vector

    根據已發表嗜水氣單胞菌的外膜蛋白基因omp的核苷酸序列設計引物，利用pcr技術，擴增、克隆了嗜水氣單胞菌l316的主要外膜蛋白基因（ momp ） ，經t a克隆，插入到pgem - t系列載體上，測序分析結果表明momp基因最長的開放閱讀框（ orf ）為1035nt ，編碼由344個氨基酸組成，分子量為36kda的主要外膜蛋白質（ momp ） 。
  9. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  10. Degreasing lipoprotein is the carrier of lipid ; after it combines with lipid and forms protein, it was transited to the organs and tissues out of the liver, along with blood

    脫脂脂蛋白是脂類物質的運載體，而與脂類物質結合成蛋白質后，隨血液運送到肝外各器官、組織。
  11. These include cell growth characteristics, expression levels, intracellular and extracellular expression, posttranslational modifications, and biological activity of the protein of interest, as well as regulatory issues in the production of therapeutic proteins. in addition, the selection of a particular expression system requires a cost breakdown in terms of process, design, and other economic considerations. section i : construction of pet22b ( + ) / hpk - 5 vector the hpk - 5 gene encoding 82 amino acid residues from c462 to c543 was recombined with the sequence of plasmid pet22b ( + ) for constructed a new expressed vector pet / hpk - 5

    方法在對hpk - 5 （ humanplasminogenkringle5 ， hpk - 5 ）因子的基因序列和蛋白質序列進行分析的基礎上，利用pcr技術分別構建其可溶性原核表達載體和不溶性原核表達載體；用pcr快速檢測法及其基因測序儀測序以鑒定pet22b hpk - 5和pbv220 hpk - 5重組質粒，用不同的感受態大腸桿菌（ e
  12. Facilitated diffusion a passive transport of molecules across a cell membrane along a concentration gradient, mediated by carrier molecules or complexes, usually proteins

    易化擴散（促進擴散） ：分子沿濃度梯度由載體分子或復合物（通常是蛋白質）作為媒介的一種被動跨膜運輸。
  13. The recombinant plasmids pbmb121l, pbmb9821l and pbmb986l were constructed after transferring bacillus thuringiensis icps gene crylaal, crylaclo and crylca from their original plasmid vectors to different plasmid vector. the relevant recombinant strains were obtained after introducing the 3 recombinant plasmids into bacillus thuringiensis plasmid - free mutant strain 8mb 171 by electroporation. the transformation and expression properties of 8mb 171 were studied

    通過將蘇雲金芽胞桿菌殺蟲晶體蛋白基因cry1aal 、 cry1ac10和cry1ca轉移至不同質粒載體上，分別構建了重組質粒pbmb121l 、 pbmb9821l和pbmb986l ，並分別導入無質粒突變株bmb171 ，篩選得到攜帶相應icps基因的重組菌株。
  14. Hegf gene with his - tag at the end, which was derived from pet22 - egf, was in - frame fused to the carboxy - terminal of polyhedrin ( ph ) gene, which included the amino - terminal 116aa coding region. the polyhedrin - egf fusion gene ( named ph - egf ) was then cut out with ecorv and ecori, and was cloned between ecorv and ecori sites of pbacpak. 8 ( the result plasmid was named pbacph - egf and the ph - egf fusing gene was right under the control of ph promoter ). the pbacph - egf structure was verified with restriction enzymes digestion and pcr

    從pet22 - egf質粒中分離出末端帶his - tag的egf基因，對位融合於多角體蛋白n端116個氨基酸基因序列的下游（命名為ph - egf ） ，並在兩段基因間設計了凝血酶xa蛋白酶切位點，經過酶切、測序等鑒定正確后，克隆至pbacpak8中，使ph - egf融合基因置於多角體蛋白（ polyhedrin ， ph ）基因啟動子控制之下，構建成重組轉移載體pbacph - egf 。
  15. Dna is the main genetic materials in most organism. the amino acids are arranged by the genetic triplet code to synthes proteins which are very important in organism

    Dna分子是絕大多數生物的遺傳信息的載體，它的三聯密碼子指導氨基酸按照一定的序列組裝成生命活動極為重要的蛋白質分子。
  16. Subcloning of 32kda proteins gene of schistosoma japonicum in the eukaryocyte expression vector

    蛋白質基因在真核表達載體中的亞克隆
  17. Methods using biocompatible alginate as microsphere material and hemoglobin as drug model, protein - loaded microspheres were prepared by an improved technology based on electrostatic droplet generation via designing components of gelation solution

    方法以生物相容性優良的海藻酸鹽為載體材料，以血紅蛋白為藥物模型，通過設計凝膠浴配方，採用靜電液滴形成工藝制備包封蛋白質藥物的微球載體。
  18. A substance that reacts with a specific antibody but cannot induce the formation of antibodies unless bound to a carrier protein or other molecule

    半抗原，不全抗原一種對某種特定的抗體起反應但除非被限制于蛋白質載體或其他分子之中，否則不能導致抗體的構成的物質
  19. Hsp70, a kind of molecular chaperone, has the main functions of taking part in protein folding, protein degredation and reparation of dna damadge and has important effects on the constructure and function of mitochondria. it has already been proved that there is a close correlation between hsp70 and the development of plants and animals. this paper deals with integrating sense and antisense cdnas of hsp70 into tobacco dna by constructing an expression vector of sense and antisense cdnas of hsp70 and gene - transforming methods - genegun bombarding and agrobacterium mediation. provided expression of hsp70 gene is inhibited by sense and antisense cdnas of hsp70 we can get male sterile plants so as to prove that antisense cdna of hsp70 leads to male sterility 1

    Hsp70是一種分子伴侶，主要功能是參與細胞有關蛋白新生肽的折疊、亞基組裝、細胞內運輸、蛋白質降解及dna損傷的修復，對線粒體結構和功能發揮重要作用，已有研究證明hsp70與動植物的發育有密切的關系，本研究將hsp70正、反義cdna構建成表達載體，並運用基因槍和農桿菌介導法將hsp70正、反義cdna導入煙草，試通過hsp70反義cdna抑制hsp70基因的表達從而創造雄性不育株，以證明hsp70反義cdna能創造雄性不育系。
  20. We constructed 3 kinds of shuttle plasmid encoding hcv structural proteins by means of molecular cloning technique ; based on it, 3 kinds of adenovirus vectors of hcv structural proteins were packed, screened and identified. we also selected and synthesized 5 kinds of ctl epitope peptides of hcv structural gene which induced 5 kinds of corresponding hcv specific ctl, which were evaluated by experiments in vivo and in vitro

    本研究利用分子克隆技術，構建了三種hcv結構蛋白腺病毒載體穿梭質粒，並以此為基礎包裝、篩選和鑒定了三種hcv結構蛋白腺病毒表達載體，選擇併合成了hcv結構基因區5條ctl表位多肽，並以此誘導了5種hcv特異性ctl ，並對其進行了體內外的實驗測定。
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