轉凝膠 的英文怎麼說
中文拼音 [zhuǎnníngjiāo]
轉凝膠
英文
transgel-
Mas precursor powders are prepared using aluminium sulfate, colloidal silica, and magnesium nitrate as raw materials via sol - gel methods, dsc - tg and xrd show that the mas precursor powders transform to cordierite completely at 1300
以硫酸鋁、硝酸鎂和硅溶膠為原料,採用溶膠-凝膠法,制得mas先驅體粉末, xrd表明,該粉末經1300的熱處理后,完全轉變為堇青石。Study of the transport of small molecules in a microemulsion - based organogel is of great significance to broaden the research area of micellar enzymology and to promote its application in biosynthesis, bio - transformation and biosensor
微乳凝膠中小分子傳質研究對于拓寬膠束酶學研究內涵、加速酶在生物合成與轉化領域中的應用、研究高性能生物傳感器等具有重要理論意義和潛在應用價值。The e. coli strain jm109 was transformed with resultant plasmid pgex - 4t - 1 / 6 - 4. 4. the transformation was induced with iptg, then the total protein from cell extract was analyzed by electrophoresis on a 8 % sds - page in order to validate the gst fusion protein, and the fusion protein is about 90kd
4 .用工ptg誘導含pgex一4t一1 / 6一4的轉化菌,提取初提物中的總蛋白,進行sds一聚丙烯酞胺凝膠電泳,檢測表達的融合蛋白大小越為gokd 。Dab served as chromagen. western blot thirty micrograms of protein extracted from untreated and bfgf, atra - induced mmscs cultures were separated on a 8 % gradient acrylamide gel and eletrophoretically transferred to a nitrocellulose membrane. the blot was probed for nse expression
4westernblot檢測誘導后細胞的nse表達情況從未經處理和經過bfgf , atra誘導的細胞中提取30爬蛋白在8的sds一聚丙烯酸胺凝膠上電泳並轉移到硝酸纖維素膜上, 4 5脫脂奶粉封閉過夜。We did the same steps for three times, so we could get the extraction using the steps mentioned. laemmli sample buffer was added to the extracted protein, which were then boiled for 5 min. the protein samples were separated by 12 % sds - page and transferred to nitrocellulose membrane
樣品蛋白經12 % sds一聚丙烯酞胺凝膠電泳分離后,轉印到硝酸纖維素膜上,與第一抗體4孵育過夜,經tbs洗滌后,再與第二抗體室溫孵育2小時, ttbs充分沖洗后,顯色觀察。Citric acid, taking the place of hno3, solutes the substance which does not solute in solution, and it acts as the ligand of metallic ionic and the hydrolysis catalyst of si ( oc2h5 ) 4, which reduces the pollution caused by no2 which forms at the decompose process of the hot treatment. by changing the means of calcine of the drier gel, choosing the suitable temperature to burn the gel, the high temperature calcine time is shorted, which solves the question of the long period calcine. so the preparation process of the matrix and composite was finished by using more lower temperature than the traditional solid state reaction and more shorter time than the traditional sol - gel process
結果使基質和復合物的制備在比傳統的固相反應法低得多的溫度下和比常規的溶膠凝膠法短得多的時間里完成;五、對基質及復合物的干凝膠、粉體和燒結體進行了ir 、 dta 、 xrd 、 seni及交流阻抗譜表徵,研究結果表明:在溶膠向凝膠的轉化過程中同時存在著正硅酸乙酯自身的聚合作用和檸檬酸鹽絡合物之間的聚合作用:干凝膠向產物粉體的轉化在400600c之間進行;基質li 。Then the gel film can be prepared by dip - coating or spin - coating technique on the surface of glass and followed by drying in the ethanol vapor
使用浸漬提拉法或旋轉塗敷法,並通過放在乙醇蒸汽中乾燥,可在玻璃表面得到均勻的凝膠膜。The results displayed that the spectra were wider than that of one single dye and there were energy transfer between c102 and rh6g. in silica films the energy transfer efficiency was higher than that in ethanol solutions
C102 : r上6gh元混合染料間存在著能量傳遞,將其摻雜到isio 。凝膠中,發現與無水乙醇溶液中相比;能量轉移效率增加,光譜i展寬,原因可能是出。The expression of lexa protein in the iptg - induced jm109 ( pza172 ) was checked by sds - page, and the survival curve of this strain was measured using colony formation assay after treatment with different doses of radiation and different concentrations of mmc
應用電穿孔技術將攜帶有抗輻射菌lexa基因的重組質粒pza172轉入大腸桿菌jm109 ,其啟動子為lacz ,用iptg誘導, sds - page凝膠電泳檢測lexa蛋白的表達。It is found that starch microgel has microporous network structure and environmental responsiveness, moreover, its volume phase transition temperature ( tc ) is around 37, which are researched by the characterization of tem, dls, light refraction and other techniques. so, starch microgel with those advantages can be expected to use as a targeting drug carrier
藉助透射電鏡、動態光散射、光折射等技術的表徵,發現所制備的澱粉微凝膠具有微孔網路結構和環境敏感性,而且相體積轉變溫度( t _ c )約37 ,所制澱粉微凝膠的這些優越性可望作為靶向藥物載體。Right now, the curator of the collector is deciding if this acceptable to carry on and begin unfolding more tiles, or if it should be discussed further by captem
現在,收集器的主管正在決定這是否可以接受並開始翻轉更多的凝膠塊,或是需要captem進一步的討論。The synthesis of nano - tio2 powder by sol - gel method and the phase transformation
凝膠法合成及晶相轉化Phase transition of poly n - isopropylacrylamide hydrogels determined by fluorescence probe technique
異丙基丙烯酰胺水凝膠的相轉變Firstly, the major ampullate glands were got from araneus ventricousus. total rna and mrna of ampullate glands were isolated and purified. double strands cdnas were synthesized in the assistance of amv - rt and e. coli dna polymerase i etc. by reverse transcription and replacement synthesis
首先剝離大腹園蛛主壺腹腺,提取總rna和分離純化mrna ,反轉錄合成cdna ,凝膠層析除去小於400bp片段,並在雙鏈cdna兩端引入ecor的銜接頭,對其進行磷酸化處理。To prepare the wild type mbl in prokaryotic system, a pair of primers was designed and synthesized, and was used to amplify mbl gene from the recombinant vector pgem - mbl that contans wild type mbl cdna. a recombinant prokaryotic expression vector, pet28 - mbl, was constructed by inserted the mbl gene into plasmid pet28 ( b ), and after transfected it into ecoli bl21 ( de3 ) and induced with iptg, recombinant mbl protein was expressed successfully
本實驗另選用了原核表達質粒pet28 ( b ) ,根據已構建好的含有mbl野生型基因的t載體pgem - mbl ,設計一對引物, pcr擴增mbl基因,凝膠回收,雙酶切pcr產物和pet28 ( b )質粒, t4連接酶連接,轉化大腸桿菌dhsa ,氨芋選擇培養挑取克隆鑒定。The process affect factors of synthesizing the new fine material tricobalt tetraoxide particles were discussed, such as temperature, the solution ph, revolution of stirrer, maturing time, washing water times, drying condition, the color change of process, were discussed, its formation mechanism and structures were systematically studied and discussed, tested the fine particles, the xrd test showed that the peaks became wide
經試驗獲得均勻凝膠沉澱法新技術優化后的工藝參數為:反應溫度控制為273 . 15k左右, ph值為9 . 0 - 9 . 5之間,攪拌轉數為300轉/分,陳化時間24hr以上,水洗去除雜質,按溫度分步烘乾。The recombinant plasmid was translated into e. coli dh5 and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 22ku protein which was equal to chicken ifn - y protein in molecular weight was expressed in e. coli dh5
將重組質粒轉化大腸桿菌dh5 ,於37誘導培養8h , sds - page凝膠電泳表明該基因在大腸桿菌中獲得了高水平表達,表達的雞ifn -融合蛋白分子量約為22ku 。Recovered the agarose and identified by agarose gelelectrophoresis. the producys of pcr fragments and pcambial303 plasmid ligation were transformed into e. coli ( dh5a ). the result of pcrof positive recombinant and restriction analysis demonstrated that the plant expressing vector of tps is attained
Pcr產物經回收后,經瓊脂糖凝膠電泳鑒定后,與pcambia1303連接並轉化大腸桿菌dh5a ,陽性重組子經pcr和限制性內切酶酶切圖譜分析,表明已獲得海藻糖- 6 -磷酸合成酶基因的植物表達載體。Starts out as a gel, giving support at the scalp and transforms into a mousse, giving volume with movement to the ends
純凈感受:剛擠出時呈現凝膠狀,給與發根支撐力,而後凝膠會轉換膨脹為慕絲狀,從發根到發尾給予輕盈蓬鬆與動感。I and hindlll respectively. after the digested products were run via agarose gel electrophoresis and transferred into nylon membrane, the southern blot was carried out using the cdna of rubber tree etrl as probe. the result of the southern blot showed that a hybridization band ( - 3. 0kb ) turned up from the ecor i digested product and another band ( - 4. 8kb ) turned up from the hindi ]
從橡膠樹pr107品系的嫩葉提取基因組dna ,用限制性內切酶ecor 、 hinofll分別酶切,瓊脂糖凝膠電泳分離並轉膜后,用克隆的橡膠樹etri基因的cdna片段作探針進行southern雜交分析,結果表明, ecor酶切在約3 okb處有一條雜交帶出現, hi 。分享友人