轉導粒子 的英文怎麼說
中文拼音 [zhuǎndǎolìzi]
轉導粒子
英文
transducing particle-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。The crystalline structure changes of pp induced by pan - milling were characterized by x - ray diffraction and raman spectroscopy. xrd analyses show that when pp was co - milled with uhmwpe, crystal transformation of pp occurred, its crystallinity and crystallite size decreased, whereas, only slight reduction of crystallinity and crystallite size were observed and no crystal transformation was found when pp was co - milled with wtr chips, and amorphization of pp was strongly enhanced by co - milling pp with iron
採用x -射線衍射研究了聚丙烯碾磨粉碎過程微觀結構變化， uhmwpe存在下pp發生晶型轉變、結晶度降低和晶粒尺寸減小；彈性材料wtr抑制pp塑性變形，碾磨導致分子堆積有序區域膨脹，晶面間距增大，結晶度下降；剛性材料金屬鐵與pp產生強烈摩擦，加快晶粒細化，晶格破裂導致非晶化。The pap gene was also transformed into wheat by pollen tube pathway - mediated and 320 grains of transgenic wheat was harvested. the seedlings of the seeds were inoculated with the gav isolate of barley yellow dwarf virus ( bydv ) by schizaphis graminum ( rondani ) and the results revealed that 33 wheat seedlings did not produce the symptom or the symptom was very mild
通過花粉管通道法將pap基因導入小麥，收獲了轉基因小麥種子320粒，將其播種，返青后，直接用麥二叉蚜將大麥黃矮病毒（ bydv ） gav株系接種到幼苗葉片上，觀察發病情況。Abstract : from a universal formula for calculating the attenuation cross section of particles in arbitrary shapes, exact expressions for calcualting the attenuation cross section of polydisperse, small spheroids, whose rotary axes are in specific status, have been derived. attenuation cross sections of both liquid and ice particles in different shapes at different wavelengths in microwave band have been computed and analyzed. the results are informative to microwave remote sensing of precipitation
文摘:從計算任意形狀粒子的衰減截面普遍公式出發，推導出了小旋轉橢球粒子群旋轉軸處于不同狀態時的衰減截面函數表達式，並計算分析各種狀態下衰減截面隨降水粒子相態、形狀和入射波波長的變化特徵，所得結果可用於降水微波遙感。In this thesis, we use the random - matrix - theory to revise the following calculating methods : ( 1 ) the heat capacity and the spin susceptibility of the normal metallic small particles ; ( 2 ) the heat capacity of the conventional metallic small particles at the low temperature using the mean field approximation method ; ( 3 ) the heat capacity of the conventional metallic small particles at the vicinity of the critical temperature using the static path approximation method
本文採用隨機矩陣理論，計算了： （ 1 ）正常態金屬小粒子的電子熱容和順磁磁化率。 （ 2 ）在平均場近似下，計算了超導金屬小粒子低溫區的電子熱容。 （ 3 ）在靜態路徑近似下，計算了超導金屬小粒子轉變溫區的電子熱容。Zhou shengyu （ computer application ） directed by chen xiaomin because of the radiation in space enviroment, the data in sram of the aerospace computer will nomally experience single event upset ( seu ) errors at a scale of small probability. had not been corrected in time, these errors would effect not only the performance of the computer system but also the transmission of the key data.
由於輻射導致的單粒子翻轉效應seu （ singleeventupset ） ，使得航天計算機上的靜態存儲器sram中的數據可能出現小概率錯誤，這種錯誤若不及時進行糾正將會影響計算機系統的運行和關鍵數據。When both genes were co - expressed in e. coli, the activity of ppsa varied from 2. 1 - 9. 1 fold comparing to control, but the activity of tkta was relatively stable ( 3. 9 - 4. 5 fold ). whatever the two genes were expressed respectively or cooperatively, both could promote the production of dahp, the first intermediate of the common aromatic pathway, but co - expression was more effective on forming dahp and screened ppt - and ptp - as more effective. the results demonstrate that co - expression of ppsa and tkta can improve the production of dahp, and what ' s more, when multigenes co - expressed, the recombinant which has coordinated enzymes activity is optimum
莽草酸途徑的最優化和整體調控基因csra的敲除正是上述改變的分子基礎，同時也為三種芳香族氨基酸的基因工程菌的構建打下了基礎; 7 .在國內外首次實現了共同途徑限制性底物關鍵酶ppsa刁無『及arog與分支途徑關鍵酶基因phea的串聯高效表達，所構建的重組質粒ptga ，其ppsa 、 tkta 、 arog 、 cm和pd的酶活分別比對照提高了3 、 2 、 2 ， 5 、 4 、 2 . 3倍，且其酶活比較協調一致; 8 .將ptga導入到篩選的基因敲除和基因替換菌株大腸桿菌31884 c甲b中，搖瓶發酵證實比以往所構建的基因工程菌株具有較高的phe產量和糖轉化率率，分別為0 . 448 %和22 . 4 % 。The absorption spectra indicate that the adsorption of dithiooxamide on the silver nanoparticles results in a red - shift in the spr band, mainly caused by the changes in the microenvironment of the metal nanoparticles and charge density alteration due to the charge transfer between the molecules and metal particles
吸收光譜結果表明銀納米粒子表面吸附二硫代乙二酰胺分子可導致金屬粒子的表面等離子體共振吸收紅移，主要與金屬粒子的微環境改變以及吸附分子與金屬間電荷轉移而導致的金屬粒子內部電子密度改變有關。This dissertation focuses on how to develop a high performance damping mechanism based on multi - energy transitions, which is independent or little dependant with the viscoelasticity of polymer matrix. three sections are mainly included in this dissertation as follows : chlorinated polyethylene ( cpe ) / piezoelectric ceramic ( pzt ) / vapor grown carbon fiber ( vgcf ) composites ; cpe / 2, 2 ' - methylene - bis - ( 4 - methyl - 6 - cyclohexylphenol ) ( zkf ) / vgcf composites ; organic hybrids made from cpe and small molecules cpe / pzt / vgcf composites : the damping composites of multi - energy transitions mechanism were produced by mixing pzt and vgcf into cpe. in this system, the mechanical vibration energy that was transmitted to cpe was found to convert into joule ' s heat through conduction paths between vgcf in the polymer matrix, so the vibration fades away
本論文主要包括三個方面的研究內容： ?氯化聚乙烯（ cpe ） /壓電陶瓷（ pzt ） /氣相生長碳纖維（ vgcf ）復合材料； ? cpe /雙[ （ 2 -羥基- 3 -環己基- 5 -甲基） -苯基]甲烷（ zkf ） / vgcf復合材料； ? cpe基有機小分子混雜型復合材料cpe / pzt / vgcf減振復合材料：利用壓電和導電填料填充高聚物基體，當復合材料受到機械振動時，通過高聚物基體將機械振動能傳遞到壓電陶瓷粒子上，利用壓電陶瓷的壓電效應，將機械能轉換成電能，則電流在導電粒子產生的迴路中流動，由於導電迴路具有一定的電阻，所以電能又轉變成熱能耗散掉，從而起到減振的目的。Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid, and induced with methanol to secrete interesting peptide. the supernate of pichia pastoris culture was analysed by sds - page and western blotting. a reactive band, which the apparent molecular weight is 36kd, can be detected with sheep anti - hcmv polyclonal antibodies
重組質粒轉化巴氏畢赤酵母， g418篩選出多拷貝插入的單克隆，甲醇誘導多拷貝插入的單克隆酵母細胞分泌目的蛋白，培養液上清經sds - page電泳分析，在蛋白質印跡中檢測到培養液上清有一表觀分子量為36kd ，能與羊抗hcmv多克隆抗體發生發應的條帶。This text completely and systematically studies the status and the development of the pyrolysis and the fluidization of biomass, which distill the bio - oil in the domestic and international area, as well as the existing problems. this thesis primarily include following aspects : ( l ), by experimenting and measuring the energy ( heat value ) and the content of c, h, n chemical element of right 20 kinds of common biomass, on the base of the experimental result, and respectively established the biomass energy predict experiment formula with the element of h and c is from change, and passed the ro. os examination, which provides the basis and convenience for flash pyrolysis fluidization device energy to convert the rate to compute with biomass energy utilization calculation ; ( 2 ), proceeded the tg and dtg experimentation equal velocity ( 10 ? / min, 20 ? / min, 40 ? / min, 60 ? / min ) heating and constant temperature heating by studying on eight kinds of biomass samples, according to the experimental data and arrhenius formula, we established the dynamics model of pyrolysis of, then, using the goast - redfern and p function, we also solved the dynamics parameters and analyze out every kind of biomass ' s frequency factor and parameters of activation energy, and established the every kind of dynamics model of pyrolysis of biomass, all of these provide the theories and basis to make sure the reactor ' s flash pyrolysis work temperature scope design and the describing of pyrolysis reactor dynamics ; ( 3 ), in order to study and ascertain the process of heat completely getting to pyrolysis time of varied size biomass particles, we observed and measured the ratio of length and diameter ( l / d ) with the varied biomass through electron microscope, we concluded the l / d ratios usually is from 5. 0 to 6. 0, the average is 5. 3 ; ( 4 ), we studied the process of biomass transiting and the theory of complete pyrolysis time with the theory of complicated heat field, we got the time ( t ) of the varied size biomass particles arriving to complete pyrolysis, and we knew that the complete pyrolysis time and the time which get to the biggest production ratio are identical, all of these studies provide the theory base for design and forecasting the flash pyrolysis reactor solid state resort time ; ( 5 ), according to the above experiment result, synthesize to make use of the engineering the mechanics, engineering the material, machine the design to learn the principle, deduce, establish the theory of rotation cone flash pyrolysis reactor material resort time ( t ) and reasonable rotation velocity ( or frequency ) relation theory ; and ( 6 ), we gave the reactor ' s smallest cone angle certain, reactor cone wall strength design theory, the reactor production ability theory, the power design method and the critical rotation velocity theory
本文較全面、系統地綜述了國內外生物質熱解液化制取生物燃油技術研究發展現狀及存在的問題，主要研究內容有： （ 1 ）實驗、測定了20種常見生物質的能量（發熱量）和c 、 h 、 n元素含量，根據實驗結果分別建立了以h和c為自變量的生物質能量預測經驗公式，並通過r _ （ 0 . 05 ）檢驗；為閃速熱解液化裝置能量轉化率計算和生物質能量利用率計算提供了依據和方便； （ 2 ）選擇了8種生物質試樣作了等加熱速率（ 10 min 、 20 min 、 40 min 、 60 min ）和恆溫加熱的tg和dtg實驗，根據實驗數據和阿倫尼烏斯公式建立了生物質熱解反應動力學微分方程，並採用goast - redfem積分法和p函數對其動力學參數進行了求解，解析出各種生物質的頻率因子和活化能參數，進而建立了各種生物質的熱解動力學模型，為科學確定反應器的閃速熱解工作溫度范圍及熱解反應動力學描述，提供了理論和依據； （ 3 ）為研究和確定不同尺度的生物質顆粒中心達到全熱解的時間，在體視顯微鏡下對不同粒度的生物質顆粒的長徑比進行了實驗觀察和測定，得出生物質的長徑比（ l d ）一般在5 . 0 6 . 0之間，平均為5 . 3的結果； （ 4 ）採用復雜溫度場傳熱學理論對生物質傳熱過程及充分熱解時間理論進行了研究，解析推導出了不同尺寸生物質顆粒中心溫度達到充分熱解溫度的時間（ t ） ，得出了理論推導的充分熱解時間與最大產油率的熱解時間相一致的結果，為閃速熱解反應器固相滯留時間設計和預測提供了理論依據； （ 5 ）根據上述實驗結果，綜合運用工程力學、工程材料、機械設計學原理，推導、建立了轉錐式閃速熱解反應器物料滯留時間（ ）與轉速（或頻率）合理匹配理論； （ 6 ）提出了轉錐式閃速熱解反應器的最小錐角設計、錐壁強度設計、生產能力設計理論和功率計算方法及臨界轉速理論等。The expression of lexa protein in the iptg - induced jm109 ( pza172 ) was checked by sds - page, and the survival curve of this strain was measured using colony formation assay after treatment with different doses of radiation and different concentrations of mmc
應用電穿孔技術將攜帶有抗輻射菌lexa基因的重組質粒pza172轉入大腸桿菌jm109 ，其啟動子為lacz ，用iptg誘導， sds - page凝膠電泳檢測lexa蛋白的表達。The chloroplast shsp gene was screened from the cdna library of tomato flower by pcr strategy and confirmed by sequencing. but difference was found at 3 bases of the sequence from the reported in genbank. then, an integrated vector prok ii of the chloroplast shsp gene and nptii gene ( a kanamycin resistant gene ) with camv35s promoter was constructed and introduced into tomato mediated by agrobacterium tumefaciens lba4404. transgenic tomato were screened by their ability of growing on media containing kanamycin
本實驗採用pcr方法從番茄花cdna文庫中克隆到葉綠體shsp基因，經測序證實與genbank中已發表的序列在編碼區相差2個堿基，其中一個堿基導致1個氨基酸的改變。將葉綠體shsp基因定向克隆于帶有組成性表達啟動子camv35s的植物表達載體prok中，凍融法轉化農桿菌lba4404 ，利用葉圓盤法對番茄進行ti質粒介導的遺傳轉化。Whenever particles interact, they can cause one another to flip
每當粒子交互作用時，可能會導致對方翻轉。Upon contraction, the rotation of the cloud and the collisions between the particles in cloud would flatten the cloud to a disk, called the
這團氣體的收縮，導至旋轉速度增加，再加上氣體粒子之間的摩擦，令氣體團變形成為碟狀，我們稱之為The intracavity photon density and initial population inversion density are assumed to be gaussian distributions instead of plan wave assumption, based on this assumption, the rate equations of diode - pumped intracavity - frequency - doubled passively q - switched laser are modified. through solving these space - dependent rate equations numerically, the results under different assumption are compared. 5
將腔內光子密度和初始反轉粒子數的平面波近似修正為高斯分佈近似，獨立地導出描述二極體泵浦內腔倍頻-被動調q激光器的速率方程，並通過數值求解該方程比較了兩種近似下脈沖特性的不同； 5 .實驗驗證了2中提出的實驗方案，並對實驗結果進行了分析。The results showed that pcr applify a 485 specific molecular band in 672 individuals, the rate of positive reaction is 60 %, southern blot result shows a strong signal in transgenic fish. it is concluded that hu - - ifn gene has been integrated and expression. it is also that foreign gene integrating position and copy number is different individuals, by elis a detecting, 23. 5 % transgenic fish " were detected hu - - ifn gene expression in all 672 transgenic fish, but expression level of hu - - dfn is significant difference in different individuals and growth period
2000年共顯微注射32197粒草魚受精卵，出苗12945尾草魚魚苗，經孵化培育最後獲得1120尾五寸左右草魚，對1120尾草魚提取血液總dna進行pcr和southern雜交檢測， 2000年轉導的草魚的pcr檢測結果，有672尾草魚即600k轉化個體的總dna能擴增出一條485kb的特異分子帶，說明轉化個體整合有huj ifn基因，經southern雜交進一步證實，轉入的抗病相關基因己在轉基因草魚體內染色體上得以整合，但整合位點沒有固定區域，整合的拷貝數存在較大差異。The analytical solutions of the gain, dispersion and population difference in the steady state of an open ladder lasing without inversion ( lwi ) system are at first given when the phase fluctuation of the driving field is considered. then the effects of the driving field phase fluctuation on the gain, dispersion and population difference are analyzed by the numerical results from the analytical solutions. our research shows that the phase fluctuation of the driving field will results in a finite line width ; with the line width increasing, the gain decreases monotonously, however, variation of the dispersion isn ? t monotonous, it has a maximum value when the line width takes some value ; the line width varying can ? t lead to change lasing from without to with population inversion
給出了有驅動場位相漲落的開放的梯型無粒子數反轉激光（ lwi ）系統增益、色散和布居數差的定態解析解，進而用得到的數值計算結果分析了驅動場位相漲落對增益、色散和布居數差的影響.研究結果表明，驅動場位相漲落將導致有限線寬;隨著線寬的增加，系統增益單調地減小，但系統色散的變化並不是單調的，當線寬取某個值時，色散有極大值;線寬的變化不能導致無反轉激光向反轉激光的轉變By driving expressions and using the method in numerical integration, the results of numerical calculations are analyzed, discussed and contrasted with different conditions, and they showed that there are evident differences in the mean attenuation, scattering, and back scattering cross sections of oblate spheroid particles when the variance is so small that it is near to zero namely it is the same as the rotatory axes are parallel with one direction and when h - polarized and v - polarized incident waves happened. these differences are depended on the relative differences in the average orientation of rotatory axes and the polarized directions of incident wave
通過公式推導和數值積分計算並對結果做了討論和分析，對比各種情況表明：扁橢球粒子群在方差很小接近為0時，與粒子群旋轉軸一致取向的情況相同，入射波水平極化和垂直極化時的平均衰減截面、平均散射截面、平均後向散射截面有明顯的不同，這決定於旋轉軸平均取向和入射波極化方向的相對差異。Sconed, the tangential shear velocity and the radial particle diameter distribution are derived according force balance in the annulus, in order to reduce or pervent the particle to remove toward the membrane surface
第二、根據粒子的力平衡理論推導出旋轉流中粒子不向膜面沉積的周向剪切速率及徑向粒徑的分佈規律，並指出徑向速度對粒子沉積的影響。分享友人