轉導細菌 的英文怎麼說

中文拼音 [zhuǎndǎoxìjūn]
轉導細菌 英文
transduction bacterium
  • 轉 : 轉構詞成分。
  • 導 : 動詞1. （引導） lead; guide 2. （傳導） transmit; conduct 3. （開導） instruct; teach; give guidance to
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 細菌 : germ; bacterium （pl. bacteria）; fungus （pl. fungi）
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  2. In transduction genetic material is transferred from one bacterial cell to another by a phage vector.

    在轉導中遺傳物質從一個細菌細胞傳給另一個細菌細胞是從噬菌體為媒介的。t
  3. A new transgenic system of gerbera mediated by agrobacterium tumefaciens

    小細胞團為受體的根癌農桿菌介導的非洲菊基因轉化體系
  4. Membrane associated energy transduction in bacteria and archaea

    細菌和古細菌中的膜相關能量轉導
  5. After the protein was electrophoresised and purified, the protein activity was detected by elisa, the protein activity of vp1 is higher than vp0 vp3. at last, the activity of vp1 made in our lab was detected with the agentia made in our lab

    將陽性重組子轉化到大腸桿菌er2566細菌內，用ipig進行誘導表達蛋白，蛋白經電泳、純化，然後用elisa方法檢測蛋白活性， vp1蛋白活性相對高於vp0 、 vp3 。
  6. Gfral was high expressed in e. coli after iptg induction. by ni2 + chelation affinity chromatography, the purified recombinant gfral protein were obtained. based on the evolutionary trace method, multiple sequence alignment and dendrogram construction were then carried out by the clustalx program and constructed a phylogenetic tree from a multiple sequence alignment for a protein family

    2 . gdnf的表達及其對pc12基因工程細胞的影響用本教研室已構建好的表達質粒pet一gdnf轉化大腸桿菌bl21 （ de3 ） ，經lmmipto誘導gdnf表達，並在niz氣nta柱上進行純化，稀釋復性后，純度達90 %以上。
  7. Thioredoxins, an ubiquitous small proteins with a redox active disulfide bridge in its conserved motif - cp ( g ) pc -, are universally distributed in eucaryote and procaryote and have a molecular mass of approximately 12kda. by its disulfide / dithiol interchange reaction, this protein can transmit the regulatory signals to seleted targets ( enzymes, transcription factors etc ) and plays an important role in many plant physiological processes that includes photosynthesis, dna synthesis, transcription, protein disulfide reduction, protein repair, filamentous phage assembly, cell apoptosis and seeds germinating and so on

    該蛋白質中含有保守的- cp （ g ） pc -氨基酸活性基序，該基序中的兩個半胱氨酸殘基可通過巰基二硫鍵的轉換實現其氧化還原狀態的變化和電子氫的傳遞，對細胞中與氧化還原相關的多種生理過程的調節起重要作用。通過同許多酶類、蛋白類、細胞內活性因子相藕連， trx能對光合作用、 dna復制、基因轉錄、細胞凋亡和生長、噬菌體組裝、蛋白質的還原和修復信號傳導等生理過程產生影響和調節。
  8. The expression vector pse380 - / iy / was constructed and transformed into e. coli dh5a, expressing hyl gene by adding iptg into the broth. the expression of hyl gene showed a 120kda protein band on sds - page gel and was found to have capability to degrade ha molecules derived from a microorganism dissolved in 0. 1 m acetate buffer solution ( ph4. 0 )

    經轉化大腸桿菌dh5a和iptg誘導表達後用sds - page電泳分析，獲得一條約120kda的表達條帶； iptg誘導表達后提取原生質膜測定透明質酸分解酶活力，表明該hyl片段的產物能夠在體外分解細菌來源的ha 。採用兩種策略滅活hyl基因。
  9. In terms with the principle of fusarium oxysporiun caused plant disease : bundles were blocked and fusarid acid killing cells was formed by hyphae so that caused water metabolism abnormal and plant wilting. in order to find out effective method of anti - fiisarium oxysporuin, long ya lillium was taken as material with plant tissue culture and genetic transformation techniques in this paper

    針對尖孢鐮刀菌的致病機理：菌絲阻塞維管束引起水分代謝失常和菌絲在植物體內產生毒素（鐮刀菌酸）損害膜結構造成代謝失常，從而導致植物萎焉。本實驗以龍牙百合為研究對象，應用細胞工程中的離體培養方法並結合轉基因技術，以期找到抗尖孢鐮刀菌的有效途徑。
  10. The transfermants with highest resistance to g418 ( 4 g / l in ypd ) were screened to study their expression level in 150 ml shaking flask. having been induced with methanol for 36 hours, the target enzyme could be examined in the supernatant by measuring amidolytic activity

    首次將大連蛇島賅蛇毒類凝血酶成熟基回克隆到表達載體ppicgk中，經電激轉化至畢氏酵母菌株gs15中，再經甲醇誘導，在150ml搖瓶畔1獲得細胞外分泌表達產物。
  11. When cooking in the microwave, cover poultry, stir, and rotate either on a turntable or manually for even cooking, as microwave heat can leave cold pockets inside the poultry where harmful micro - organisms such as bacteria and viruses can survive

    當用微波爐烹調時，需要包好禽肉並通過轉盤或者手動進行攪拌和轉動，使其加熱均勻，因為微波烹調可能會使禽肉中存有未被加熱的部分，這會導致有害微生物如細菌和病毒的存活。
  12. The cultured cell suspensions tested by western - blotting showed that transfected cells could express the exogenous gene and secrete human lactoferrin protein, with mw of 34 kd. the highest amount detected with elisa reached 65mg / l medium / 105 cells. the recombinant hlf protein has the effect of inhibiting e. coli proliferation, whose activity is 1. 4 - 1. 8 times higher than the commercially available hlf

    誘導后，培養液上清通過western - blotting分析證明，轉染細胞表達並分泌出人乳鐵蛋白，分子量為34kd ； elisa檢測重組蛋白最高表達量為65mg l培養基10 ~ 5細胞；抗菌實驗表明，所獲得的重組人乳鐵蛋白具有抑制大腸桿菌生長的作用，而且比人乳鐵蛋白標準品作用更強。
  13. Pcr, pcr - southern blot analysis, southern dot blot analysis of lettuce dna confirmed that adw gene had been integrated into the plant genome. the results also showed that the transformation frequency of pb - adw was higher than that of pbg - adw, which suggested that camv35s promoter would be better than pi ii promoter in the case of transgenic lettuce

    （ 3 ）細胞核載體pb - adw 、 pbg - adw均採用農桿菌介導法將adw導入萵苣，細胞核轉化獲得了生長良好的抗性萵苣植株，經pcr 、 pcr - southern 、 southern斑點雜交分析證實， adw基因已整合到萵苣基因組中。
  14. In transduction genetic material is transferred from one bacterial cell to another by a phage vector

    在轉導中遺傳物質從一個細菌細胞傳給另一個細菌細胞是從噬菌體為媒介的。 t
  15. The mrna and protein expression were assayed by rt - pcr and sds - page. the results were found that the specific 740bp dna bases of il - 6 was detected by rt - pcr in the recombinant bacteria and a new protein band was found in sds - page with molecular mass of about 49 kda which is consisted of a 23 kda protein deduced from the il - 6 gene sequence and gst ( 26 kda )

    以iptg誘導融合蛋白表達，經rt - pcr檢測發現740bp的特異性il - 6條帶；通過sds - page分析，在49kd處出現gst ? pil6融合蛋白條帶，其表達量占總細菌蛋白量的30 ，證明豬il - 6基因得到了正確轉錄和表達。
  16. We obtained our transgenic material, the rice suspension cells, by inducing embryonic rice callus. then we constructed the expression vector pca - ced9, and transferred ced - 9 gene into the rice callus and embryogenic suspension cells. the work of using hygromycin selective medium to obtain regenerated plants is still going

    構建了用於水稻中表達的載體pca - ced9 ，通過農桿菌eha105轉化導入水稻愈傷組織和水稻懸浮細胞，經潮黴素（ hym ）篩選，以期望獲得抗性植株，目前該工作仍在進行中。
  17. In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee

    本實驗用pcr擴增hcv結構區基因，克隆到桿狀病毒表達載體pfastbacl中，構建成重組轉座載體pfb1 - cee ，轉化dh10bac大腸桿菌感受態細胞，篩選陽性菌落，抽提大分子質粒dna ，獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ，脂質體介導轉染sf9昆蟲細胞，出現細胞病變后，收集含有重組桿狀病毒顆粒的培養上消，重新感染sf9細胞，收集sf9細胞，進行12 . 5 sds -聚丙烯酰胺凝膠電泳，可見表達的蛋白條帶。
  18. This reduces their efficiency in delivering iron to your baby, meaning that more iron is available to harmful bacteria ( leading to digestive problems ) and less is available to baby

    這降低了向機體轉運鐵的效率，意味著更多的鐵被有害的細菌利用（導致消化系統的問題） ，而機體利用的鐵就更少。
  19. These results are very important for us to further understand the genetic background, biological characteristics, evolutionary rule and the anti - schistosomajaponicum mechanism of microtus fortis at the molecular levels. the specific base changes of the dna fragme nt between the two subspecies are probably correlative with the animal immigration, survival conditions, and species evolution. the cdna library of microtus fortis bone marrow cells was transferred in situ to nylon membrane, which was divided into eight equals ( ga ~ - gh )

    利用已經建立的東方田鼠骨髓細胞質粒cdna文庫，將cdna文庫轉化菌落印跡至尼龍膜，將膜均分成8份（ ga gh ） ，制備基因池，分別培養、提取基因池質粒dna ，通過lipofect - 2000脂質體轉染技術，將基因池質粒dna導入hek293細胞， 48h后收集轉染細胞上清液，即條件培養基。
  20. Transfer of genetic material or characteristics from one bacterial cell to another by a bacteriophage or plasmid

    轉導作用通過噬菌或質料把基因材料或特徵從一種病菌細胞轉到另一種病菌細胞。
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