轉導載體 的英文怎麼說

中文拼音 [zhuǎndǎozǎitǐ]
轉導載體 英文
transduction vector
  • 轉 : 轉構詞成分。
  • 導 : 動詞1. （引導） lead; guide 2. （傳導） transmit; conduct 3. （開導） instruct; teach; give guidance to
  • 載 : 載Ⅰ名詞（年） year : 一年半載 six to twelve months; six months to a year; 三年五載 three to five ...
  • 體 : 體構詞成分。
  • 載體 : [化學] carrier; supporter; isotopic carrier
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  2. Finally 4 right clones was obtained from 864 transformants by pcr detection. sequencing analysis showed that the hsa gene have been introduced into the right position of the human a - lactalbumin yac. furthermore, works to optimize the conditions of introducing the recombinant yac into goat f ibroblast via cell fusion was explorded

    經pcr檢測，從864個轉化子中獲得了4個陽性克隆，測序表明，人血清白蛋白基因已正確的導入到人-乳白蛋白基因yac的特定位點上，並獲得了可進行細胞融合的重組yac載體。
  3. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  4. This paper describes a strategy that has developed to transfer the cdna of tobacco mnsod gene into the commercially important breeding line - baoding alfalfa via agrobacterium infection. transgenic alfalfa plants have been generated that overproduce a nicotiana plumbaginifolia l. manganese superoxide dismutase ( mnsod ). the results domenstrated that baoding alfalfa is an important breeding line which easily amenable to genetic transformation

    本研究採用我國農藝性狀優良的豐產苜蓿品種保定苜蓿，通過農桿菌介導的轉基因方法，使用特定的質粒載體pchlsod將煙草mnsod基因的cdna序列導入保定苜蓿中，說明保定苜蓿是一種易於遺傳轉化的優良苜蓿育種品系。
  5. Marek " s disease virus ( mdv ) phosphoprotein 24 ( pp24 ) gene was amplified from md11 strain by polymerase chain reaction ( pcr ). then we cloned it into the downstream of gst gene according to the right open reading frame ( orf ) in pgex - 6p - l vector

    本研究將型mdvmd11株的pp24基因的完整orf克隆入原核表達載體pgex - 6p - 1中，重組質粒pgex - pp24轉化bl21宿主菌后，經iptg誘導表達。
  6. As a popular vegetable, tomato is rich in vatamin a, b, p and other nutrients ; among them, lyxopene can prevent prostate gland tumor. so introducing stilbene synthase gene into tomato to get new health - protection tomato will have important social and economic effect. our research is made up of four parts ; the cloning and sequencing of stilbene synthase ; tomato genetic transforming mediated by agrobacterium - lba4404 ; quantification of resveratrol in transgenic tomato by hplc analysis

    本研究包括四個方面的內容：芪合酶基因（ stilbenesynthasegene ）的克隆與全序列分析；芪合酶基因（ stilbenesynthasegene ）植物表達載體的構建；農桿菌lba4404介導的番茄遺傳轉化： hplc技術分析轉基因植株中表達白藜蘆醇的含量。
  7. We clone a 1. 3kb promoter sequence of the homologous gene in arabidopsis by pcr. this promoter is shown to direct the specific expression of the reporter gene, b - glucuronidase ( gus ), in trichomes of arabidopsis. promoter deletion analysis reveal that the region from - 300 - - 1 bp is sufficient to direct trichome - specific expression

    對其進行缺失突變，構建5個缺失表達載體轉基因擬南芥，葉片gus定量測定分析表明- 300bp ? - 1bp序列就可以指導gus基因在表皮毛細胞中特異表達，說明這段序列可能含有指導此啟動子在擬南芥表皮毛細胞進行特異表達的核心序列。
  8. The regeneration system of soybean cytoledon node and agrobacteriunr mediated transformation method is the first selection at present. in the second part of this experiment, the expression vector prok2 containing npt ii and ssnhx1 ( na + / h + antiporter ) gene from suaeda salsa was introduced into soybean cytoledon nodes by gene transformation mediated by agrobacterium tumefaciens, and kanamycin resistant transgenic p lants were obtained by screening in selective condition

    本實驗第二部分通過農桿菌介導法將含npt -和鹽地堿蓬na ~ + h ~ +反向轉運蛋白基因（ ssnhx1 ）的表達載體prok2導入大豆子葉節中，經過含km的篩選培養基連續篩選，獲得了ssnhx1轉基因植株，篩選劑卡那黴素的適宜濃度是50mg . l ~ （ - 1 ） 。
  9. This gene, artificially put under the control of camv 35s, was introduced into tobacco with the aid of agrobacterium, and the transgenic plants obtained were identified by gus staining, pcr and southern blot analysis

    將arge與組成型啟動子camv35s相連，構建植物表達載體，通過農桿菌介導轉化煙草。經過gus染色、 pcr及southern鑒定獲得了轉基因植株。
  10. The resulting plasmid, named prok - sod2, was mobilized to agrobacterium tumefaciens strain gv3101 used for plant transformation. the yeast sod2 gene was introduced into arabidopsis thaliana ( ecotype landsberg erecta ) by agrobaterium tumefaciens - mediated transformation with floral - dipping method under the control of camv 35s promoter. transformants were selected for their ability to grow on medium containing kanamycin ( 30mg / l ), several homozygous lines that were all tolerant to kanamycin were selected and used for further molecular and physiological determination

    本實驗將sod2基因構建到植物表達載體prok中，導入農桿菌后，進行植物遺傳轉化，實現其在擬南芥中過量表達，在含30mg l的卡那黴素的培養基上篩選獲得純合轉基因株系，自交一代獲得足夠的純和轉基因種子后，對其進行了分子生物學的驗證及生理指標的檢驗。
  11. The plasmid plncxhlf which is combining with 2366bp human lactoferritin ( hlf ) cdna complete sequence and 800bp regulating sequence upsteam by the - lactoglobulin gene promoter is prepared by normal steps. then it is used in the following experiments. the recombinant plasmids plncxhlf were introduced into mouse mammary tumor cell line ma - 782 by liposome entrapment

    本研究將含有人乳鐵蛋白（ hlf ）基因2366bpcdna序列完整序列和800bp的奶山羊-乳球蛋白基因5 』端調控序列的乳腺特異表達重組載體plncxhlf按常規方法提取、純化、 pcr 、酶切鑒定后，用於細胞表達和精子介導轉基因動物的研究。
  12. Ssmapkk transformations were screened on media with kanamycin ( 30mg / l ). nineteen individual kanamycin resistant plants were obtained. t2 plants were checked for integration of foreign gene by counting ratio of the number of tolerant plants to the number of non - tolerant plants on selection medium with kanamycin ( 30mg / l )

    將ssvp和ssmapkk的全長cdna分別克隆入植物表達載體pcambia1300和prok中，導入根瘤農桿菌gv3101后，由花浸泡法進行擬南芥遺傳轉化，轉化ssvp鹽地堿蓬ssop和ssmapkk基因的克隆與功能鑒定的擬南芥在含潮黴素（ 25mg ）的ms培養基上篩選，獲得t ；代轉基因植株。
  13. Both the mature genes of gloshedobin and gussurobin were cloned into the vector pet - 32a ( + ), strain bl21 ( de3 ), to study their expression in prokaryotic cell. the gene was expressed under t7 promoter with a fusion protein partner of thx. tag and a 6x his. tag at its n - terminal. having been induced by iptg for 4 hours, the recombinant enzyme was examined in the cytoplasm by sds - page analysis

    將大連蛇島蝮蛇和長白山白眉蝮蛇毒類凝血酶基因分別克隆到大腸桿菌表達載體pet - 32 （ a ） +中，在t7啟動子下表達出融合蛋白，融合部分為硫氧還蛋白，位於類凝血酶基因上游，並在其n端帶6xhistag標簽以利於表達產物的分離純化，經熱激轉化至宿主菌bl21 （ de3 ）中， iptg誘導斗小時后收獲菌體。
  14. The transfermants with highest resistance to g418 ( 4 g / l in ypd ) were screened to study their expression level in 150 ml shaking flask. having been induced with methanol for 36 hours, the target enzyme could be examined in the supernatant by measuring amidolytic activity

    首次將大連蛇島賅蛇毒類凝血酶成熟基回克隆到表達載體ppicgk中，經電激轉化至畢氏酵母菌株gs15中，再經甲醇誘導，在150ml搖瓶畔1獲得細胞外分泌表達產物。
  15. A binary plant expression vector with osg6b " driving report gene gus was constructed and transferred into tobacco via agrobacterium tumefaciens mediation

    將克隆的啟動子與報告基因gus相連，構建植物表達載體，通過農桿菌介導轉化煙草。
  16. Pcr, pcr - southern blot analysis, southern dot blot analysis of lettuce dna confirmed that adw gene had been integrated into the plant genome. the results also showed that the transformation frequency of pb - adw was higher than that of pbg - adw, which suggested that camv35s promoter would be better than pi ii promoter in the case of transgenic lettuce

    （ 3 ）細胞核載體pb - adw 、 pbg - adw均採用農桿菌介導法將adw導入萵苣，細胞核轉化獲得了生長良好的抗性萵苣植株，經pcr 、 pcr - southern 、 southern斑點雜交分析證實， adw基因已整合到萵苣基因組中。
  17. The e2 genes above of the prevalent strain ( guangxi yulin strain ) were cloned respectively into secreted expression vector ppic9k of eukaryotic expression system p. pastoris and transformed into p. pastoris by electroporation after linearization, 25 high - copied transformants were obtained by g418 screening. it was proved that the e2 genes were integrated stably into chromosome of p. pastoris by dot blot and dna sequencing

    豬瘟病毒e2基因的真核表達：分別將csfv兩個代表株的e2基因克隆入畢赤酵母（ p . pastoris ）分泌型表達載體ppic9k中，酶切線型化后電穿孔導入p . pastotis進行整合，經g418篩選得到25個高拷貝轉化子，經dna斑點試驗和dna測序證明外源基因e2穩定地整合到p . pastoris染色體中。
  18. The chloroplast shsp gene was screened from the cdna library of tomato flower by pcr strategy and confirmed by sequencing. but difference was found at 3 bases of the sequence from the reported in genbank. then, an integrated vector prok ii of the chloroplast shsp gene and nptii gene ( a kanamycin resistant gene ) with camv35s promoter was constructed and introduced into tomato mediated by agrobacterium tumefaciens lba4404. transgenic tomato were screened by their ability of growing on media containing kanamycin

    本實驗採用pcr方法從番茄花cdna文庫中克隆到葉綠體shsp基因，經測序證實與genbank中已發表的序列在編碼區相差2個堿基，其中一個堿基導致1個氨基酸的改變。將葉綠體shsp基因定向克隆于帶有組成性表達啟動子camv35s的植物表達載體prok中，凍融法轉化農桿菌lba4404 ，利用葉圓盤法對番茄進行ti質粒介導的遺傳轉化。
  19. In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee

    本實驗用pcr擴增hcv結構區基因，克隆到桿狀病毒表達載體pfastbacl中，構建成重組轉座載體pfb1 - cee ，轉化dh10bac大腸桿菌感受態細胞，篩選陽性菌落，抽提大分子質粒dna ，獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ，脂質體介導轉染sf9昆蟲細胞，出現細胞病變后，收集含有重組桿狀病毒顆粒的培養上消，重新感染sf9細胞，收集sf9細胞，進行12 . 5 sds -聚丙烯酰胺凝膠電泳，可見表達的蛋白條帶。
  20. The recombinant pcr technic was used to introduce a linking peptide klgggg to the site between scfv single chain form of the monoclonal antibody sz - 51 specific for the glycoprotein gmp140 on activated platelet membrane and uk32 low molecular weight form of pro - urokinase, to make the scfv - linker - uk32 chimeric gene. this gene was cloned into the transfer vector pbacpak9, and cotransfected with bacpak6 bsu36i digest into sf 9 cells. the fusion protein was secreted into the medium. in the fifth day after the cotransfection, the supernatant of the medium showed 107 iu ml fibrinolytic activity, higher than 25 iu ml fibrinolytic activity of scfv - uk32. elisa showed that the supernatant had the binding activity to activated platelet. wastern blotting also indicated that the supernatant could bind to the monoclonal antibody of urokinase b chain

    為了提高重組導向溶栓分子scfv - uk32的溶纖活性，通過重組pcr方法在編碼scfv與uk32的堿基之間引入編碼klgggg連接肽的堿基序列，並克隆到轉移載體pbacpak9上，通過與線性病毒dna bacpak6 bsu36i digest共轉染到昆蟲細胞sf 9內，進行表達。表達產物分泌到上清中，共轉染后第5d天用纖維平板法測得sf 9細胞上清溶纖活性達到107 iu ml ，比未引入連接肽的scfv - uk32的表達活性25 iu ml高。
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