轉染試劑 的英文怎麼說
中文拼音 [zhuǎnrǎnshìjì]
轉染試劑
英文
transfection reagent-
In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company
實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。With the purpose of better understanding some weak links in this subject, we comprehensively and systematically compared the effects of some typical inhibitors and their different combined ways in inhibiting cd bioavailability in purple soil, further studied the laws and affecting factors of cd ' s transfer and transformation in soil - plant system, revealed the mechanisms inhibitors function, and explored the practicable inhibitor prescriptions and their usage. the results are expected to provide reliable techniques for controlling cd pollution of agricultural soils in chongqing and it is also significant to ameliorate soils polluted by other kinds of heavy metals. comprehensive methods including pot experiment, laboratory culture experiment, kinetic and thermodynamic adsorption - desorption experiment were adopted in this study, and the acidified purple soil was chosen to test, considering that cd is highly active in this soil
本研究針對重慶市農業土壤的污染狀況，選取鎘活性較高的酸化紫色土為供試土壤，採用盆栽試驗、培養試驗、化學熱力學與動力學吸附試驗相結合的方法，系統研究和比較多種調控劑及其不同組合方式對鎘生物活性的效應，並揭示鎘在土壤?植物系統中的遷移轉化規律及影響因素，進一步分析調控劑的作用機理，探尋具有實際應用價值的調控劑配方及合理使用的方法，這不僅可以為重慶市農業土壤鎘污染的防治提供可靠的技術保障，而且對于其它重金屬污染的治理也有一定的理論意義。2. construction of chimeric mtb8. 4 / hil - 12 eukaryotic expression plasmid ( 1 ) construction of pci - neo - mtb8. 4 - linker ( pml ) and pci - neo - ms - linker ( pmsl ) mtb8. 4 - linker and ms - linker gene ( without stop codon ) were pcr amplified by using two oligonucleotides designed to generate nhe i and mlu i restriction sites at the 5 " and 3 " ends of the amplified fragments, respectively
3 .重組質粒在真核細胞中的表達: pm 、 pms 、 pmi和pmsl重組質粒用lipofectaminatmzo0o脂質體轉染試劑轉染cos一7細胞，進行瞬時表達， 48小時后，用rl 』 - pcr檢測目的基因在mrna水平的表達;用westemblotting檢測hil一12在蛋白質水平的表達。Although the deelopment of relatiely non - toxic immunosuppressie or tolerance - inducing regimens will be required to justify clinical trials using pig organs, recent adances in our understanding of the biology of xenograft rejection and zoonotic infections, and the generation of alpha1, 3 - galactosyltransferase - deficient pigs hae moed this approach closer to clinical application
盡管用豬的器官去驗證臨床試驗需要相對無毒的免疫抑制劑或致耐的方法，但隨著近來我們對異種移植排斥生物學及動物傳染病感染的深入理解，以及1 ， 3半乳糖轉移酶缺陷豬的產生，異種移植更接近臨床應用。分享友人