轉肽基作用 的英文怎麼說
中文拼音 [zhuǎntàijīzuòyòng]
轉肽基作用
英文
transpeptidylation-
Researches of schistosomiasis vaccines have gone more than 60 years, approximately including from the stages of dead vaccine and live vaccine ( irradiated attenuated cercariae vaccine ) to gene engineered vaccine, etc. many different forms of vaccines have been tested in animal models, including gluthathione s - transferase, paramyosin, irv - 5, triose phosphate isomerase, sm23, fatty acid binding protein ; which were considered promising by who / tdr. but none of them steadily accomplished the pre - set target level of 40 % protection. in order to enhance the protective capacity further, it is essential to develop novel vaccine antigens and / or vaccine adjuvants
血吸蟲病疫苗研究已有60多年的歷史，大致經歷了死疫苗、活疫苗（照射致弱尾蚴疫苗）和基因工程疫苗等研究階段，產生了一些who / tdr推薦認為很有希望的疫苗候選分子，如谷胱甘肽- s -轉移酶（ gst ） 、副肌球蛋白（ sm97 ） 、照射致弱疫苗抗原5 （ irv - 5 ） 、磷酸丙糖異構酶（ tpi ） 、曼氏血吸蟲膜內在蛋白（ sm23 ）和脂肪酸結合蛋白（ fabp ， sm14 ）等，但其對宿主的保護作用均不甚理想，未能穩定地達到40或以上的保護力水平，因此有必要繼續尋找新的疫苗抗原分子和/或疫苗佐劑，進一步提高其保護力。The artificial antibacterial peptide - ceme gene was designed according to the codon with the highest frequency in pichia pastoris, then the ceme gene was integrated into the chromosome of pichia pastoris strain - gsl 15 by electroporation
我們根據人工設計的新型抗菌肽ceme序列設計ceme基因，重組到酵母胞內表達型的整合載體phild2中，通過電轉化作用將ceme基因整合至酵母宿主gsll5染色體上。To get in vivo evidences that apoplast calmodulin con 1d regulate plant growth and development process, a chimeric secretion form of calmodulin binding peptide, which contains a signal peptide, a calmodulin binding domain and a c - myc epitope was constructed. the chimeric gene was introduced into arabidopsis. it was expected that the overexpression of this chimeric protein could be secreted into cell wall and bound to apoplast calmodulin, which could reduce the apoplast calmoduin concentration to make an apoplast camodulin " antisense " plant. by observing the potential phenotype change of apoplast calmodulin " antisense " plant, the in vivo function of apoplast calmodulin on plant growth and developmental process could be speculated
但這些多是採用生理學手段和藥理學方法而得出的體外（ invitro ）實驗結果，為了取得質外體cam在植物生長發育過程中發揮重要作用的invivo實驗證據，根據動物中的一些研究方法，本實驗設計並構建了帶有信號肽、 cam結合肽（ can小肽） 、 epitope （ c - myc ）融合基因的載體，並將融合基因通過真空滲入法轉入擬南芥，預期過表達的融合蛋白將會被分泌到細胞外並與質外體cam相結合，這樣就會抑制質外體cam的功能，從而可以構建質外體cam的「反義」植株，通過觀察質外體cam 「反義植株」的表型改變，就可以推斷質外體cam在植物生長發育過程中的功能。3. calcitonin gene - related peptide attenuates glutamate - induced inhibition of pulmonary surfactant lipid synthesis ( d cgrp could reverse the decrease of [ 3h ] - choline incorporation induced by glu with dose - dependence ; ( 2 ) cgrp could block the decrease of the content of cct a mrna induced by glu in lung tissue ; ( 3 } cgrp could reduce the impairment of the ultrastructure of at ii cells induced by glu ; ( 4 ) cgrp could reverse the increase of mda content and decrease of sod level induced by glu in cultured lung explants, respectively ; ? grp had no significant effect on nos activity and increase of no production induced by glu
降鈣素基因相關肽減輕谷氨酸所致肺表面活性物質脂質合成抑制的保護作用降鈣素基因相關肽grp ）可顯著減輕0所致肺組織h一膽堿摻入pc量的降低，並且呈劑量依賴保護效應； cgrp可逆轉gill所致cctqinrna含量的降低； cgrp可減輕o所致肺11上皮細胞超微結構的損傷； cgrp可逆轉o所致肺組織勻漿中mda含量增多、 sod水平降低的效應，並可逆轉q所致肺組織ldh釋放增多的效應； cg販對gill引起的nos活性和no含量的升高均沒有顯著性影響。It is known from studies using brefeldin a ( bfa ) that disruption of tgn can block the cytotoxicity of ricin, suggesting that the intracellular compartment may be an important part of the uptake pathway. ricin enters the cells by receptor - mediated endocytosis, followed by translocation across the membranes of intracellular organelles. in this study, a trans - golgi retention peptide signal yqrl was fused to the c - terminus of ricin a chain ( rta ) by polymerase chain reaction
本實驗為重點研究反式高爾基體在毒素細胞內轉運過程中的作用，我們設計了一個反式高爾基體的靶向信號肽基因yqrl （ tyr - gln - arg - leu ）連接在rta分子的羧基端，用原核表達蛋白體系，純化並測定rta - yqrl與rta蛋白對hela （人宮頸癌細胞） ， wish （人羊膜上皮細胞） ， skov - 3 （人卵巢癌細胞）三種細胞的毒性作用。分享友人