轉質子酶 的英文怎麼說

中文拼音 [zhuǎnzhízi]
轉質子酶 英文
transprotonase
  • 轉 : 轉構詞成分。
  • 質 : Ⅰ名詞1 （性質; 本質） nature; character; essence 2 （質量） quality 3 （物質） matter; substance;...
  • 子 : 子Ⅰ名詞1 （兒子） son 2 （人的通稱） person 3 （古代特指有學問的男人） ancient title of respect f...
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 質子 : [物理學] proton; uron; hydrion; merron質子泵 proton pump; 質子層 proton sphere; 質子轟擊 proton b...
  1. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  2. Study of the transport of small molecules in a microemulsion - based organogel is of great significance to broaden the research area of micellar enzymology and to promote its application in biosynthesis, bio - transformation and biosensor

    微乳凝膠中小分子傳質研究對于拓寬膠束酶學研究內涵、加速酶在生物合成與轉化領域中的應用、研究高性能生物傳感器等具有重要理論意義和潛在應用價值。
  3. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  4. The principle and method for enzymatic synthesis of gallic acid, isolation and selection of the aspergillus niger strains, characteristics of this biotechnology, products quality of gallic acid and the uses in domestic food and pharmaceutical industries are briefly introduced

    摘要概述了黑麴黴單寧酶酶法轉化五倍子單寧酸生產沒食子酸的原理和方法、酶源菌種的分離和選育、工藝技術的特點、產品質量規格及在國內食品、醫藥行業相關部門的應用等。
  5. On the other hand, - 6 fatty acid desaturase injection assay will be performed to check whether a metabolic pathway is established. methords of research three plasmids vector with expression elements are used to establish a eucaryotic expression vector by restriction enzyme cutting and ligation. this vector is used to pronucleus microinjection

    本實驗以pegfp - n1質粒為骨架載體，用酶切連接的方法構建一個順序含有- actin啟動子、 fad2cdna 、 sv40polya加尾信號的真核表達載體，雙切線性化后回收，使用回收的表達載體經原核顯微注射生產轉基因小鼠。
  6. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  7. Meanwhile, in order to improve the e. coli with ability of using sugar. we have recombined the vecter of parg25 a which have ability of using sugar with the gene of sucrase length of 7800bp

    同時，為了促進所構建的大腸桿菌具有蔗糖利用能力，我們構建了含有大小為7 . 8kb的蔗糖水解酶基因sacc重組質粒，篩選得到含有兩種基因的重組子，並轉化大腸桿菌，使大腸桿菌獲得蔗糖利用能力。
  8. For protein identification, proteins spots of interest on the gels stained with colloidal coomassie brilliant blue g - 250 were excised, digested in - gel with trypsin, and analyzed by peptide mass fingerprinting ( pmf ) with matrix assisted laser desorption / ionization - mass spectrometry ( maldi - ms ). erp60, trypsinogen, proelastase, lipaseandso on were identified. this will enable us to present an overview of the proteins expressed in rat pancreas tissues and lays the basis for subsequent comparative proteome analysis studies with pancreas development

    從中隨機選擇一些差異蛋白質點，進行基質輔助激光解吸-電離飛行時間質譜（ maldi - tof - ms ）測定其膠內酶解后的肽質指紋圖譜，用mascot軟體查詢swiss - port數據庫，初步鑒定為一些與生長發育、物質代謝、細胞因子、信號轉導等有關的蛋白質，如erp60 、 trypsinogen 、 proelastase 、 lipase等。
  9. Many glycoproteins of lower and higher eucaryotes are attached to the plasma membrane by means of a glycosylphosphatidylinositol ( gpi ). gpi - anchored proteins are synthesized on membrane - bound ribosomes. upon translocation of the pro - protein across the endoplasmic reticulum membrane, gpi : protein transamidase ( gp1t ) recognize and removes the carboxy terminal gpi signal sequence and attaches a gpi molecule to the newly exposed carboxy terminal amino acid

    Gpi化前體蛋白在依附於膜的核糖體上合成，當其易位穿過內質網（ er ）膜后，被gpi ：蛋白質轉酰胺基酶（ gpit ）識別， gpit在移走其羧基端gpi信號序列的同時將gpi分子連接至新生成的氨基酸位點上。
  10. Along with the development of the cytobiology and the molecular biology, and thoroughly research of the biophysics, the biochemistry, the genetics and immunology, it has cultivated the modem biological technology, such al genetic engineering, cellular engineering, enzyme engineering, fermentation engineering and so on, to change biology characteristic to carry on the material transformation, has formed the front biological examination technology : the dna probe, the pcr technology, the molecular mark, the bioluminescence technology, genechip technology and so on the widespread application of these advanced biotechnologies in dairy industry baa impelled the dairying technical transformation, and has been having vital significance to dairy production, research and dairy product security

    摘要隨著細胞生物學和分子生物學的發展及對生物物理、生物化學、遺傳學和免疫學研究的深入，培育了基因工程、細胞工程、酶工程、發酵工程等改變生物特性進行物質轉化的現代生物技術，形成了dna探針、 pcr技術、分子標記、生物熒光技術、基因晶元技術等前沿性的生物檢測技術，其在乳品工業中的廣泛應用，推動了乳業的技術變革，對乳品生產、研究和乳品安全意義重大。
  11. Thioredoxins, an ubiquitous small proteins with a redox active disulfide bridge in its conserved motif - cp ( g ) pc -, are universally distributed in eucaryote and procaryote and have a molecular mass of approximately 12kda. by its disulfide / dithiol interchange reaction, this protein can transmit the regulatory signals to seleted targets ( enzymes, transcription factors etc ) and plays an important role in many plant physiological processes that includes photosynthesis, dna synthesis, transcription, protein disulfide reduction, protein repair, filamentous phage assembly, cell apoptosis and seeds germinating and so on

    該蛋白質中含有保守的- cp （ g ） pc -氨基酸活性基序，該基序中的兩個半胱氨酸殘基可通過巰基二硫鍵的轉換實現其氧化還原狀態的變化和電子氫的傳遞，對細胞中與氧化還原相關的多種生理過程的調節起重要作用。通過同許多酶類、蛋白類、細胞內活性因子相藕連， trx能對光合作用、 dna復制、基因轉錄、細胞凋亡和生長、噬菌體組裝、蛋白質的還原和修復信號傳導等生理過程產生影響和調節。
  12. Levels of fasting blood glucose and 24h urinary microcontent of albumin 24 h malb were determined dynamically ; the serum glycosyl hemoglobin hba1c was determined after the last medication ; the ultrastructural changes of kidney were observed by transmission electron microscope ; the expressions of collagen, fibronctin, laminin ln, and the ecm metabolism influencing factors, including mmp2, tissue inhibitor of metalloproteinase timp2, transfer growth factor 1 tgf 1 in renal tissue were detected by immunohistological chemistry and image collecting analytical system

    動態檢測各組大鼠空腹血糖fbg 24h尿微量白蛋白24h malb ，末次給藥后測定大鼠血漿糖化血紅蛋白hba1c透射電鏡觀察各組大鼠腎臟超微結構改變，應用免疫組化技術及圖像採集分析系統測定各組大鼠腎臟組織中型膠原c纖維連接蛋白fn層粘連蛋白ln的表達，測定影響ecm代謝的基質金屬蛋白酶2 mmp2基質金屬蛋白酶抑制劑2 timp2及轉化生長因子1 tgf 1的表達。
  13. In those instances nuclei as well as cytoplasms came together and the hybrid cells made perfectly functioning enzymes that expressed the genetic code of two types of animal

    在那些例子中，細胞核和細胞質融合在一起，雜交細胞製造出了表達兩種動物遺傳密碼、完美運轉的酶。
  14. Construction of male sterility expression vector by integration of artificial sense and antisense cdnas of hsp70 into puc18 and puc19 respectively, we can obtain psc and pac. tapertal specific expression promoter ta29 and terminator nos are connected directionally with sense and antisense cdnas of hsp70 extrected and purified from psc and pac., then integrated into puc18 and puc19, by which we can build sense and antisense cdna nos ( respectively named plasmid 650 and plasmid 651 ) of ta29 - hsp70. for the sake of better screening and examination of transformed gene, we cut plasmid 650, plasmid 651 and 3301 ( containing gusgene bar screening marker gene ) with hindiii and ecor i enzymes, then connect purified fragments of 650and 651 with plasmid 3301 to construct the vector 3301 + 650 and 3301 + 651. corroboration of whether sense and antisense cdna - nos is integrated into plasmid3301 can be made by plate screening and enzye - cutting analysis

    分別將從psc 、 pac回收純化的hsp70正、反義cdna與絨氈層特異表達啟動子ta29及nos終止子定向連接，然後插入到puc18 、 puc19中，構建成花藥特異表達的ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos ，分別稱作650和651質粒。為了更好地對轉化子進行篩選和檢測，用hind和ecor分別對650 、 651及3301質粒（含gus報告基因和bar篩選標記基因）進行酶切，將從650和651回收純化的目的片段與3301質粒進行連接，再對重組子進行平板篩選和酶切分析確定ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos插入到3301質粒中，構建成3301 + 650和3301 + 651表達載體。
  15. Selected one of the 14 strains - s93, s93 dna was digested partially with sau3a i and 2 ~ 3kb fragments were collected and inserted into puc 18, then transformed into dh5 a. filtering the clone with hybridization in situ, a 1 kb frament clone has been cloned

    使用sau3ai對基因組dna進行不完全酶切，回收2 3kb片段，與puc18質粒連接轉化大腸桿菌，利用地高辛標記探針，使用菌落原位雜交篩選轉化子；篩選到包含有約1kb外源片段的轉化子。
  16. After digested with ecori and noti, the obtained dna fragment was linked with ppic9k by t4dna ligase and then the recombinant plasmid was transformed into competent dhscc. after positive transformants were sieved out by pcr, digesiting analysis and sequencing were also used to confirm the positive result more

    所得的dna片段經ecor和not雙酶切後用t _ 4dna連接酶與ppic9k載體進行連接，然後導入大腸桿菌dh5 ，用pcr法篩選陽性轉化子，並用雙酶切和序列測定方法鑒定重組質粒。
  17. Major difference of hn proteins lies in no. 18 - no. 75 amino acid residues on n - terminal which includes three active sites of hn protein. the influence on biological action, caused by the difference of hn protein, is needed to study further. hn gene has only four glycosylation sites, which is 1 or 2 less on amount than that of other strains. so, this difference can be take on as a natural mark of ndv b95 strain furthermore, the fragment encoding hn gene was excised from the positive clone pgem - hn with sail and saci enzyme, and purified by agrose gel fraction method. then the fragment was subcloned into the pet - 28c expressing vector digested by sail and saci restriction enzyme

    作者將正確的重組克隆質粒pgem - hn用saii 、 saci雙酶切，電泳回收目的片段，將其亞克隆到經saii 、 saci雙酶切處理的pet - 28c中，轉化大腸桿菌tgi感受態細胞，得到的轉化子經pcr鑒定和酶切分析，篩選出符合閱讀框的重組子，構建成重組表達質粒pet - hn ，並在大腸桿菌bl _ （ 21 ） （ de _ 3 ）宿主菌中成功地表達了含目的蛋白的融合蛋白，融合蛋白的分子量約66kd ，加入iptg誘導8h后，蛋白表達幾乎達到最高水平。
  18. The objective of this research is to transform the cloned phytase gene into pichia pastoris in order to obtain high - yield phytase - producing strain and to optimize the engineered yeast media recipes for the scale - up production of phytase. main results of this research are as follow : 1, xba i - linized recombinant plasmid ppic9k - phya was transformed into pichia pastoris by gene pulser. 98 positive transformants showing measurable phytase activities were screened on md plates and ypd plates containing g418. they all grew quickly on both md plates and mm plates, which proved their phenotypes of methanol utilization were mut +

    主要實驗結查如下：西南農業大學碩士學位論文1 、用乃ai酶切攜帶植酸酶基因表達片段的重組質粒ppicgk夕句） a ，回收dna ，用genepulser電擊轉化畢赤酵母，塗布md平板，又用含不同濃度g418的ypd平板進行抗性篩選，得到98個可檢測到植酸酶活力的陽性轉化子，它們在md 、 mm平板上均表現快速生長，說明其甲醇利用表型是mut干。
  19. Main works and their important results are showed as following. firstly, the cdna encoding bullfrog gh is amplified by use of the total rna, extracted from adult bullfrog ' s pituitary, as the template, pi ( 5 ' - cggatcc atg gct tca ggg tta ggc ) and p2 ( 5 ' - cgaattc tta aaa ggt gca gtt gct ) as the primer pair and one particular cdna product, 660bp, was obtained after the rt - pcr - the product was purified by using dna agarose gel extraction method. after the purified cdna and pmd18 - t vector were ligated at 16 over night, the ligation products were transformed into e. coli strain dh5a and then the transformants were screened by clone pcr method

    首先，以成體牛蛙的腦垂體總rna為模板，進行rt ? pcr擴增得到約660bp的特異性pcr產物帶，切下瓊脂糖凝膠中的特異產物帶進行cdna膠回收，將cdna與pmd18 ? t載體進行t ? a克隆連接並轉化到dh5 e . coli后，進行菌落pcr 、質粒酶切鑒定，篩選出陽性菌株，測序結果經blast分析，與已報道的牛蛙生長激素基因高度同源，證實此陽性克隆為牛蛙生長激素基因轉化子，命名為pbfgh 。
  20. 3. the amplified dna fragment was then ligated into the hindlll and ecori sites of pmg36e. the ligation mixture was transformed into jm109 for the initial cloning. the recombinant plasmid pmg36e / nisa isolated from jm109 transformants were analyzed by restriction enzyme digestion

    將酶切並純化后的pcr擴增產物與大片段連接並轉化e . colijm109 ，在含紅黴素的lb平板上篩選到含有重組質粒的轉化子。
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