通讀蛋白 的英文怎麼說
中文拼音 [tōngdòudànbái]
通讀蛋白
英文
read-through protein-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。It has been shown that this occurs in bacteria in that antisense rna sets up base pairs with a certain messenger rna ( m - rna ) precisely where the ribosomes would start their reading
這些蛋白質通過核糖體聚合在一起，且他們是通過沿著的閱讀和翻譯信使rna氨基酸的序列而行成，這就決定了各種蛋白質在細胞內的不同功能。Doxycycline gums up the assembly line on which proteins are made, whereas ciprofloxacin stops the dna message about how to make each protein being read in the first place, so there is plenty of scope for interference between the two
脫氧土霉素是通過黏附在蛋白質生產線來阻止細菌蛋白質合成的，而環丙沙星是通過阻抑指導蛋白合成的dna遺傳信息的讀取過程來祈禱抗菌作用的。Initially, a polyclonal antibodies was used to screen the cdna expression library to isolate pf40 gene from millet. sequence analysis revealed that the pf40 gene contained an open reading frame ( orf ) encoding a protein with 75 % - 35 % sequence identity to the zips ( zinc or iron transporter proteins ) gene family. software analysis of the pf40 gene character showed that pf40 protein had a eight trans - membrane region structure, pf40 protein was also rich in leucine ( 18. 4 % ) and the protein was a hydrophobic protein
序列分析表明該基因具有一個開放閱讀框（ openreadingframe ， orf ） : genbank中查詢該基因編碼的蛋白與zips （ zincorirontransporterproteins ，離子通道蛋白）基因家族的蛋白有75 - 35的相似性；軟體的分析結果顯示該基因編碼的蛋白為一個有8個跨膜區的跨膜蛋白； pf40蛋白為疏水蛋白，以上的特徵也許是pf40基因發揮作用的基礎。分享友人