連續傳代培養 的英文怎麼說
中文拼音 [liánxùzhuàndàipéiyǎng]
連續傳代培養
英文
continuous passage culture- 連 : Ⅰ動詞1 （連接） link; join; connect 2 （連累） involve （in trouble）; implicate 3 [方言] （縫） ...
- 續 : Ⅰ形容詞（連接不斷） continuous; successive Ⅱ動詞1 （接在原有的后頭） continue; extend; join 2 （...
- 傳 : 傳名詞1 （解釋經文的著作） commentaries on classics 2 （傳記） biography 3 （敘述歷史故事的作品）...
- 代 : Ⅰ動詞1 （代替） take the place of; be in place of 2 （代理） act on behalf of; acting Ⅱ名詞1 （歷...
- 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
- 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
- 連續 : continuation; succession; series; continuity; continuing; running; continuous; successive; contin...
-
According to these problems, we adopt to the method of mending material, optimize to fermentation media and partly ferment condition. finally, we excogitate a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified. with the plasmid pbv220 - ifnr, pbv220 - hgfa, pbv220 - hgfb, pbv220 - hpk5 that expresses serve as the model, adopting the biostat - c15l of b. braun company, utilize the method of mending material to ferment, through optimization fermentation media and optimization partly ferment condition ( ventilate quantity, stir speed, mend material speed ), eventually establishment a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified
以我室構建並穩定表達的重組質粒pbv220 - - ifn 、 pbv220 - hgf 、 pbv220 - hgf 、 pbv220 - hpk5為模型，分別從不同的表達宿主菌中篩選出一種適合大規模生產的菌種bl21 （ de3 ） ，該工程菌株連續傳代100代表達質粒不丟失，表達量穩定；採用b . braun公司的biostat - c15l自控發酵罐，運用分批補料技術分別進行四種工程菌的高密度發酵，通過優化工程菌發酵的培養基配方及優化部分發酵條件（通氣量、攪拌速度、補料速度） ，最終建立一種適于目的基因高效表達的高密度發酵工藝模式。2. isolation and cloning of mouse embryonic germ cells of icr species primordial germ cells ( pgc ) were isolation from 8. 5 - 15. 5 dpc ( days post coitum ) embryos. eg cell lines with the characteristic of murine es cell line were established and continuously cultured to 6th passage
Icr小鼠eg細胞的分離克隆研究本試驗以icr品系小鼠胚胎8 . 5 15 . 5dpcpgc為材料，經傳代培養，獲得能連續而穩定傳至6代的，具有胚胎幹細胞諸多特性的eg細胞系。The vaccine was developed by passaging eiav strain liaoning ( eiav l ) in donkeys in vivo, obtaining a donkey - adapted virulent equine infectious anemia virus ( eiav da ) and then passaging it in donkey leukocyte cultures for more than 120 times in vitro
該毒株是將eiavl株（ eiavl ）通過驢體傳代，使其毒力明顯增強，然後在驢白細胞培養物上連續傳代致弱獲得的。Bovine viral diarrhea virus were propagated by mdbk cells. after five to six continuously and rapidly passing, the cells emerged evident cytopathic effect. then the infected cells and its solution were harvested
Bvdvnadl株屬于致細胞病變型毒株，用mdbk細胞培養增殖bvdv ，經5 - 6次連續傳代至出現明顯的細胞病變。Methods : isolated rabbit bmsc by adhesion method and cultured it at 37c, 5 % co2 incubator. graphic the growth curve after observed the growth of primary cells and passage cells. cultured bmsc in calcified solution and observe its potential of osteogenic
方法：貼壁法分離第四軍醫大學碩士學位論文兔骨髓基質幹細胞， 37 ， 5 % co :培養箱培養，觀察原代及傳代細胞的生長發育情況，並繪制生長曲線;在礦化液中連續培養，觀察其骨形成能力。3 after incubated with 5 - bromo - 2 - deoxyuridine ( brdu ) for 7 days, newborn cells derived from regenerative neurospheres were immunostained. 4 the cells were collected by centrifugation separation and put into poly - 1 - lysine - coated terasaki wells, which were randomly divided into one of three groups of 24 wells cells each
4將傳代培養的神經球離心收集，轉移至24孔培養板，隨機分為3組，每組24孔細胞，置5 % coz 、 370c培養箱，連續培養7d ，觀察神經球的誘導分化。Among all pgc - derived cultures, it was found that one derivation under went 12 passages
具有分化潛能的pgcs能在體外連續傳代培養達12代。Continuous passage of neurospheres was used to isolate, culture nscs from dentate gyrus of hippocampus of newborn rats and to observe the self - renewal of neurospheres, which were immunostained with nestin
採用神經球連續形成法從新生大鼠海馬齒狀回分離、培養神經幹細胞，通過連續傳代培養觀察其自我更新能力。分享友人